The speciation and antimicrobial susceptibility pattern of coagulase negative Staphylococci in a tertiary care hospital in Mumbai (original) (raw)
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International Journal of Current Microbiology and Applied Sciences, 2018
Coagulase-Negative Staphylococcus (CoNS) comprise an ever-expanding group of bacteria whose medical importance has emerged in the past decades. They have become one of the most frequent nosocomial pathogens isolated from blood cultures, often in association with intravascular devices and as a cause of deep-seated prosthetic implant infections (Parameswaran et al., 2011). These organisms isolated from clinical specimens with increasing frequency. So this study planned to isolate and antibiogram of Coagulase Negative Staphylococcus (CoNS) bacteria in various clinical specimens at tertiary care teaching hospital at Jaipur. The present study was carried out on total 60 Coagulase Negative Staphylococcus (CoNS) were isolated from 400 different clinical samples like Urine, Blood, Pus, Sputum, High Vaginal Swab, Semen, Nasal Swab and ET Secretion etc. from all age's group. Conventional bacteriological methods were used for identification Coagulase Negative Staphylococcus (CoNS) and susceptibility testing was performed with the help of the Modified Kirby-Bauer disc diffusion method as per CLSI guidelines 2014. This study comprises of 60 isolates of Coagulase Negative Staphylococcus (CoNS) from total of 400 clinical specimens collected from in patients admitted to various departments during a period of 6 months. Staphylococcus epidermidis was the most common isolate (38.33%) while Staphylococcus saprophyticus (35%) was the second most common isolated followed by Staphylococcus haemolyticus (15%), Staphylococcus lugdanensis (5%), Staphylococcus schleiferi and Staphylococcus xylosis have same account (3.33%). Among all clinical samples Urine yield maximum isolates of CoNS i.e. 26 (43.33%) followed by Blood 10 (16.67%), Pus 9 (15%), Sputum 6 (10%), High Vaginal Swab 5 (8.33%) etc. Most sensitive drug against CoNS were Vancomycin (100%) followed by Linezolid (86.67%), Amikacin (71.67%). The most common species identified was Staphylococcus epidermidis. Resistance to Penicillin and Amoxyclav was high and none of the isolates showed resistance to Vancomycin. Early detection of MRCoNS and reduction in indiscriminate use of antibiotics like Vancomycin are the only suitable approaches to reduce the emergence of these drug resistant strains.
2017
Coagulase negative staphylococci (CoNS) were once considered as skin commensals and dismissed as culture contaminants, are now being recognized as significant nosocomial pathogens. CoNS are also associated with resistance to several commonly used antimicrobial agents. It is important to identify CoNS up to the species level, as the epidemiology, the pathogenecity and drug resistance varies from species to species. Study was carried for period of 12 months. CoNS isolates from various clinical samples, collected from different sites were subjected to biochemical characterization. Antimicrobial susceptibility test were done by using Kirby Bauer disc diffusion method. Out of eighty six isolates Staphylococcus epidermidis 29(33.7%) was most common isolate, followed by S.hemolyticus 23(26.7%), S.saprophyticus 11(12.8%), and S.hominis 9 (10.5%). Other CoNS isolates were S.lugdunensis 6(7.0%) S. schleiferi 4(4.7%), S.warneri 2(2.4%), S.cohnii 1(1.2%). Antibiotic susceptibility testing showe...
Clinical and antimicrobial profile of Coagulase Negative staphylococci in a tertiary care hospital
International Journal of Research in Medical Sciences
Background: Coagulase negative staphylococci (CoNS) are gaining importance because of their role as pathogens in certain clinical conditions and their marked resistance to antibiotics. Their species distribution and slime production has important correlation with the antimicrobial susceptibility pattern. Aim of this study was to determine clinically significant CoNS, their species distribution, slime production and antimicrobial susceptibility pattern in a tertiary care hospital.Methods: Identification, speciation and antimicrobial sensitivity testing were performed using standard microbiological techniques. Slime production was also tested by microtiter plate. Antimicrobial susceptibility testing was performed by modified Kirby Bauer method as per the CLSI guidelines.Results: A total 204 (49.88%) CoNS were found to be clinically significant. Percentage of clinical significance was high in urine isolates (88.88%) followed by pus (47.78%) and blood (45.56%). The most common CoNS infe...
IP Innovative Publication Pvt. Ltd., 2017
Coagulase negative staphylococci (CONS) species identification is difficult for most clinical laboratories. The schemes proposed by Kloos and Schleifer is the reference method used for the identification of staphylococcal species and subspecies. It requires the utilization of a large number of biochemical tests. Antibiotic susceptibility testing was done by Kirby-Bauer disk diffusion method. Out of the total 51 CONS isolates subjected for species identification the most common species identified was S. epidedermidis (58.82%). S. cohinii and S. haemolyticus being the second and third most frequently encountered species. Common source of most species was pus followed by blood and urine. S. epidermidis and S. cohnii showed resistant to multiple antibiotics. The most effective antibiotic for all the species of CONS were linezolid, teicoplanin and vancomycin. So CONS isolated from clinical samples should always be considered as potentially significant pathogen. The species identification with antibiogram will be of benefit for both epidemiological and patient care purpose.
Speciation and antibiogram of Coagulase Negative Staphylococci (CoNS) in a Tertiary Care Hospital
IP Innovative Publication Pvt. Ltd., 2018
Introduction and Objectives: CoNS which were considered as contaminants with minimal significance, have emerged as important agents of human disease. The recognition of increasing antimicrobial resistance among CoNS isolates constitutes a potentially worrisome emerging characteristic. Hence, this study was done to speciate and determine the resistant pattern of CoNS. Materials and Methods: 122 clinically significant CoNS isolates were obtained from various clinical samples between August 2016 to July 2017. These samples were processed conventionally. Results: Of the 122 CoNS species isolated majority were from pus. Identification of CoNS showed S.epidermidis 70 (57.4%), as the most frequently isolated species. Antimicrobial susceptibility pattern of the isolates showed maximum resistance to Penicillin. Methicillin resistance was seen among 19 (15.6 %) isolates. But all the isolates showed 100% sensitivity to glycopeptides and Linezolid. Conclusion: Meticulous identification, speciation and resistant pattern of the isolate is of paramount importance in clinical management of patients. Keywords: Coagulase negative Staphylococci(CoNS), Contaminant, Antimicrobial susceptibility pattern, Methicillin resistance
JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH, 2022
Introduction: Coagulase Negative Staphylococci (CoNS) are common opportunistic pathogens. They are increasingly being recognised as nosocomial pathogens and are associated with multiple antimicrobial resistance mechanisms particularly methicillin resistance. Therefore, rapid and reliable identification upto the species level is necessary to predict the potential pathogenicity or antibiotic susceptibility of each clinical isolate. Aim: Isolation and speciation of CoNS from various clinical samples, and to determine their antibiotic susceptibility pattern. Materials and Methods: This study was a hospital-based crosssectional study carried out in the Department of Microbiology, Jawaharlal Nehru Institute of Medical Sciences (JNIMS), Imphal, Manipur, India, during the period from September 2017- August 2019. CoNS isolates were identified using conventional microbiological procedures and speciation was done following the scheme of Kloos and Schleifer. Antibiotic susceptibility was determ...
Panacea Journal of Medical Sciences, 2023
Abstract Background: Coagulase negative staphylococci have been recognized as one of the important cause of hospital acquired infections in recent past. It has become difficult to manage these infections due to emergence of multidrug resistance to them. The aim of this study was to determine antibiotic sensitivity trend of all Coagulase negative Staphylococci isolates using modified Kirby- Bauer disk diffusion technique. Materials and Methods: To assess the antibiotic sensitivity for coagulase negative staphylococci, Kirby-Bauer disc diffusion method on Muller-Hinton agar was used. The study findings were interpreted on the basis of CLSI standards. All coagulase negative staphylococci isolates were given a predetermined panel of antibiotics. Methicillin-resistant coagulase negative staphylococci were identified using Cefoxitin disk. Results: The sensitivity to Linezolid, Vancomycin and Rifampicin was found in all 500 isolates of coagulase negative staphylococci in our study. However, they were resistant to Penicillin G (65%), Cefoxitin (56%), Ciprofloxacin (57%), Levofloxacin (32%), Gentamicin (21%), Erythromycin (67%), Clindamycin (60%), Cotrimoxazole (51%) Tetracycline (9%). Conclusion: As CoNS have emerged as an important agent of hospital acquired infections, hence there is need of identification, speciation and resistance pattern of isolates for better management of patients. Keywords: Coagulase Negative Staphylococci, Methicillinresistant coagulase negative Staphylococci, Antibiotic susceptibility
Nepal Journal of Obstetrics and Gynaecology, 2014
Aims: This study was undertaken to determine the prevalence and pathogenicity of coagulase-negative staphylococci in clinical samples and to study the antibiotic-sensitivity pattern of the coagulase-negative isolates. Methods: A prospective study was conducted over a period of two years on patients admitted in the Sher-i-Kashmir Institute of Medical Sciences. Blood, urine, pus, catheter tips, cerebrospinal fluid and peritoneal fluid samples of patients who fulfilled the criteria for being labeled as nosocomial were cultured. Results: One hundred and six strains of coagulase negative staphylococci were isolated from the samples and among them 90 isolates were identified as Staphylococcus epidermidis (84.90%). Most of the coagulase-negative staphylococci isolates were resistant to penicillin, cephalosporins and fluoroquinolones. Methicillin resistance was found in a significant number of coagulase-negative isolates. All the isolates were found to be sensitive to vancomycin. Conclusion...
Study Of Coagulate Negative Staphylococci (Cons) & Their Antibiotic Resistance Profile
Objective:-Prevalence of CoNS is increasing day by day all over the world including India. They are generally associated with device associated infections and are often resistant to a number of antibiotics. It is a matter of concern for clinicians as such infections are difficult to treat. The aim of this study is to find out implications of CoNS in hospital admitted patients and to determine their resistance profile, so that necessary steps could be taken to prevent spread of such infections and formulate an effective antibiotic policy. Material and Method :-Various clinical samples submitted to microbiology lab for culture and sensitivity were processed as per standard laboratory methods for isolation and identification of CoNS. Antibiotic sensitivity profile was determined for these isolates by Kirby-Bauer disc diffusion method as per CLINICAL & LABORATORY STANDARD INSTITUTE (CLSI) guidelines 1 .Result:-One hundred (100) isolates of CoNS were obtained during processing of various clinical samples such as blood, sputum, urine, pus and swabs from wound, vegina & throat.. Maximum number of CoNS was isolated from blood samples followed by respiratory tract, urine, pus and swabs from wound, vagina and throat. They were subjected to antibiotic sensitivity test by disc diffusion method. Sensitivity test was also done against cefoxitin and Novobiocin. 72% of CoNS were found to be Cefoxitin resistant while 28% were Cefoxitin sensitive .Infection was more common in males than females and preponderance was more in middle age group.