Minimum inhibitory concentration of nano-silver bactericides for beneficial microbes and its effect on Ralstonia solanacearum and seed germination of Japanese Cucumber (Cucumis sativus) (original) (raw)
Related papers
Open Journal of Animal Sciences, 2013
Silver nanoparticles have mainly been studied for their antimicrobial potential against bacteria, but have also proven to be active against several types of viruses, even against pebrine as well. Methods: Petri dishes and transmission electronic microscope were applied to observe silver nanoparticles against bacteria strains and nuclear polyhedral viruses (NPV) and cytoplasmic polyhedral viruses (CPV). For biological test, 10 8 CFU/mL NPV with strong pathogenicity, and the NPV samples treated with 1:100 ratio of "Sumerian Silver" for 8 hrs were sprayed on the mulberry leaves, respectively, for silkworm rearing. Conclusion: Silver nanoparticle solution showed a strong bactericidal effect against both G + and G − bacterial pathogenic strains from Bombyx mori and mulberry. Under transmission electronic microscope (TEM), Bacillus sp. Samples showed light particles in the cells when treated with silver nanoparticles, in contrast, untreated samples showed homogeneity contents. Further, NPV particles showed no significant differences between treated and untreated samples, but CPV showed strong effects that almost all CPV were collapsed. For biological test, "Sumerian Silver" treated NPV showed no diseased silkworm but nearly all silkworms were dead with no treatment. It seems like that silver nanoparticles were proved to be more effective against CPV than that of NPV. The significant differences between two antivirus or virucidal mechanism should be greatly aroused the scientific interest.
Applied Microbiology and Biotechnology
The main measure worldwide adopted to manage plant bacterial diseases is based on the application of copper compounds, which are often partially efficacious for the frequent appearance of copper-resistant bacterial strains and have raised concerns for their toxicity to the environment and humans. Therefore, there is an increasing need to develop new environmentally friendly, efficient, and reliable strategies for controlling plant bacterial diseases, and among them, the use of nanoparticles seems promising. The present study aimed to evaluate the feasibility of protecting plants against attacks of gram-negative and gram-positive phytopathogenic bacteria by using electrochemically synthesized silver ultra nanoclusters (ARGIRIUM‑SUNCs®) with an average size of 1.79 nm and characterized by rare oxidative states (Ag2+/3+). ARGIRIUM‑SUNCs strongly inhibited the in vitro growth (effective concentration, EC50, less than 1 ppm) and biofilm formation of Pseudomonas syringae pv. tomato and of...
Silver Nanoparticle: Synthesized and Antimicrobial Activity on Target Plant Pathogens
Current Journal of Applied Science and Technology, 2020
The growth rate of agricultural production is reducing worldwide every year due to mainly biotic and abiotic stresses including plant diseases. Various organic and inorganic methods are being used to protect plants from disease causing pathogens. Among them, use of pesticides is the most prevalent one incurring millions of dollars on pesticides globally for control of plant diseases. In recent years, environmental hazards and ill effects caused by indiscriminate use of pesticides have been widely discussed. Therefore, agriculture scientists are finding an alternative antimicrobial compounds such as nanoparticles for the management of diseases with least adverse effect on nature and ecosystem. Herein we reviewed the synthesis, antimicrobial efficacy and compatibility of silver nanoparticles which could help to develop the novel technology for crop protection.
Bioengineering
Aloe vera, Mentha arvensis (mint), Coriandrum sativum (coriander), and Cymbopogon citratus (lemongrass) leaf extracts were used to synthesize stable silver nanoparticles (Ag-NPs) by green chemistry. UV–vis spectrophotometry, X-ray diffraction (XRD), thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and energy dispersive X-ray (EDX) spectroscopy techniques were used to characterize these biosynthesized nanoparticles. The data indicated that the silver nanoparticles were successfully synthesized, and the narrower particle size distribution was at 10–22 nm by maintaining a specific pH. As a short-term post-sowing treatment, Ag-NP solutions of different sizes (10 and 50 ppm) were introduced to mung bean seedlings, and the overall increase in plant growth was found to be more pronounced at 50 ppm concentration. The antibacterial activity of Ag-NPs was also investigated by disc diffusion test, minimum inhibitory concentration (MIC), and minimum bactericidal concentrati...
Effects of Dissolved Silver and Silver Nanoparticle on Soil Microorganisms
Journal of Soil and Water Sciences, 2017
Silver nanoparticles (AgNPs) are the most commonly used man-made nanomaterial in consumer products. They are merged into a vast collection of products due to their particular broad-spectrum anti-microbial action. On the other hand, their anti-bacterial properties may pose a significant environmental risk. This study was undertaken to assess whether the risk that AgNPs and dissolved Ag pose to soil microorganisms gained from nano-or dissolved-silver forms. The antimicrobial effect of green manufactured AgNPs (46.2±23.2 nm) and Ag + (as AgNO 3) on soil microbes were studied using disc diffusion assay. Soil microbial growth was assessed by the measurement of inhibitory zone area (mm 2) as a function of different AgNPs and AgNO 3 concentrations, 0.00, 250, 500, 1000 and 2000 mgl-1. The results of antimicrobial effect of AgNPs showed 10 fold of magnitude comparing with Ag + at lower concentrations (250 mgl-1). The theoretical predicted unity of inhibition zone ratio for AgNPs/Ag ions implies that AgNPs equates to about 5 times antimicrobial effects of Ag +. Although the current results confirm that Ag antimicrobial effect is a nano-specific effect, different bacteria isolated from different soils should be used to explore their physico-chemical properties in mitigating AgNPs toxicity effects.
Inspite of very wide application of different types of nanoparticles in different commercial fields including pharmaceutical and food industries, the toxic effects of these nanoparticles on living systems have not been clearly established. Increased applications of nanoparticles by human beings lead to accumulation of more and more nanoparticles in the environment which ultimately affect the ecosystem. The current study focused on phytotoxicity of silver nanoparticles to V.radiata and B.campestris crop plants. Effect on seedling growth by nanoparticles is comparatively more than ions solution during treatment period. The test plants exposed to nanoparticle shows that the average particle size was about 25.3 nm which was determined by X-Ray Diffractions spectrophotometer. In addition, result from Fourier Transform Infrared spectrometer reported no change in chemical composition on the basis of vibrations of functional group of molecules in treated root samples. However, Scanning Electron Microscope images revealed depositions of isolated small and spherical nanoparticles in root cells. The nanoparticles appeared to be either filling the epidermal crypt or adhering onto the root surface of test plants.
Advanced Science Letters, 2014
The rapid production and application of nanoparticles (NPs) have resulted in substantial release of nanosized particles into the environment. Nanoparticles (NPs) is being used in several products including food and agriculture sectors, is gaining importance in recent years. The antimicrobial properties of silver have made it as major ingredient in several agricultural products. Silver nanoparticle (AgNPs) is one of the most widely used NP in biological research due to their antimicrobial properties and do not produce any adverse effects on plants.
The rapid production and application of nanoparticles (NPs) have resulted in substantial release of nanosized particles into the environment. Nanoparticles (NPs) is being used in several products including food and agriculture sectors, is gaining importance in recent years. The antimicrobial properties of silver have made it as major ingredient in several agricultural products. Silver nanoparticle (AgNPs) is one of the most widely used NP in biological research due to their antimicrobial properties and do not produce any adverse effects on plants.
Applied Sciences, 2022
Nanotechnology plays an important role in many fields of science and the economy. A special example of nanostructures is silver nanoparticles (AgNPs) created following the principles of green chemistry, i.e., without the use of toxic reducing compounds. The common tomato (Solanum lycopersicum) is a popular vegetable whose germination and growth process are studied by using, e.g., in vitro cultures. The aim of the experiment was to evaluate the inhibitory effect of the biodegradable gels containing silver nanoparticles on the development of microbial infection and to evaluate their influence on the germination degree of Tomato (Solanum lycopersicum) seeds in in vitro plant cultures. Based on macroscopic and microscopic observations, all experimental samples showed the presence of Gram-positive bacilli as well as mould fungi of the genus Rhizopus, Alternaria and Aspergillus. The study showed that the biocomponents containing silver nanoparticles obtained by using xylose as a reducing ...
Applied Biochemistry and Microbiology
screening of aqueous extracts of herbaceous plants that grow on the territory of the Russian Federation with their subsequent use for the biosynthesis of silver nanoparticles (SNPs) in accordance with the principles of green chemistry has been performed. Extracts from the leaves of three plants (Mentha piperita L., Melilotus officinalis, and Archangelica officinalis) promoted efficient synthesis of SNPs, reducing the silver cation from AgNO 3. SNP formation was tested on a spectrophotometer. Atomic force microscopy showed that plant extracts cause the formation of SNPs of different sizes-from 10 to 80 nm. Scanning electron microscopy revealed SNPs with various shapes and sizes; they were most commonly spherical. The obtained nanoparticles had a bactericidal effect on Escherichia coli K-12 and Pseudomonas aeruginosa PA01 (the latter being more resistant), and they suppressed the formation of E. coli biofilms. The obtained data show that the use of extracts from three herbaceous plants provides a readily available and ecologically safe method for the production of SNPs with antimicrobial activity.