Antibodies to Neospora caninum in the blood of European bison (Bison bonasus bonasus L.) living in Poland (original) (raw)

A review of Neospora caninum in water buffalo ( Bubalus bubalis )

Veterinary Parasitology, 2015

Neospora caninum is an obligate intracellular parasite causing abortion and reproductive failure in ruminants. Here, the seroprevalence of Neospora DNA and anti-Neospora antibodies and the correlation between the DNA and the antibody using polymerase chain reaction (PCR) and a new developed whole cell-based enzyme-linked immunosorbent assay (ELISA) in water buffalo (Bubalus bubalis) were investigated. To determine the level of anti-Neospora antibody, 83 serum samples were collected from buffaloes in the northwest of Iran. Plates were coated with 2 × 10 6 whole Neospora tachyzoites and the anti-Neospora antibody level was determined by calculating the ratio of sample/positive control (S/P) optical densities (ODs) in the ELISA. All samples with the ration of 0.50 or above were accounted as positive. To confirm the presence of Neospora DNA, the serum samples were directly subjected to PCR and nested PCR for detection of Neospora NC5 gene without the DNA isolation process. A total number of 83 buffalo serum samples were examined for the presence of anti-N. caninum immunoglobulin G and Neospora DNA. All samples with the S/P ratio of 0.50 or above (16 samples, 19.27%) were also positive for Neospora DNA. All samples with OD less than 0.50 (34 samples, 40.96%) were negative for Neospora DNA. However, 33 samples with the S/P ratio of bellow 0.50 (39.75%) showed a significant level of antibody. A 100% correlation was observed between high levels of the anti-Neospora antibody and Neospora DNA in the serum of water buffalo, and the whole N. caninum tachyzoites have the potency to be used as antigens for detection of the parasite in ELISA.

Neospora caninum in buffaloes Nasir 1

Seroprevalence of Neospora caninum in dairy buffaloes (Bubalus bubalis) was assessed in the Lahore District of Punjab Province, Pakistan. The study revealed an overall prevalence of 54.7% for N. caninum antibodies determined through a competitive enzyme-linked immunosorbent assay performed on randomly collected serum samples. The highest prevalence was observed in buffaloes .3-5 yr of age (64.1%), followed by 57.9% for 5 to 6 yr olds, and 55.8% in 1-yr-old neonates, with high probability of infection under intensive dairy farming conditions. The pattern of prevalence was closely associated with the season as reflected by the highest prevalence (70.5%) in summer (May-August) and lowest (39.6%) in winter (November-January). Aborting buffaloes illustrated significantly higher (78.9%) exposure compared with non-aborting dams (59.8%). Prevalence in animals with canine contact was significantly higher (60.3%) than without contact (48.1%). This is the first reported prevalence of N. caninum in Pakistan.

Evidence of Neospora caninum infection in buffaloes (Bubalus bubalis) from Northwestern Romania

Parasitology Research

Neospora caninum is a heteroxenous parasitic Apicomplexan protozoan, able to infect a variety of domestic and wild animals, mainly associated with reproductive disorders and abortions in susceptible species. Considering the importance of Romania in the European buffalo industry and the severe economic losses caused by N. caninum infection in livestock, the aim of the present study was to assess the occurrence of neosporosis in buffaloes raised in household and farming systems in northwestern Romania. Overall, 197 serum samples were tested for the presence of N. caninum-specific antibodies by ELISA. Additionally, from 74 slaughtered buffaloes, diaphragm digest, heart, and lymph node samples were tested for the presence of N. caninum DNA. The overall seroprevalence was 68.5%. The seroprevalence was significantly higher in adult animals compared to calves and young ones and in the household system compared to farm. By nPCR, six samples were found to be positive for N. caninum DNA, of which three were serologically negative. The present study reveals a high prevalence of N. caninum-specific antibodies in buffaloes in northwestern Romania and to our best knowledge, it is the first one performed in Romania.

Assessment of Neospora caninum seroprevalence in buffalo in Tabriz city, north-west of Iran

Buffalo Bulletin, 2017

Neospora caninum is an intracellular parasite which causes abortion in buffalo worldwide. The aim of this study was to determine the seroprevalence of Neospora caninum in buffalo in the province of east-Azerbaijan in northwest Iran. Blood samples were collected from 236 buffalo in the province of east-Azerbaijan for determining the seroprevalence of Neospora caninum. A total of 236 serum samples were tested for anti-neospora antibodies. Serum samples were analyzed for antibodies against N. caninum antigen using a commercial N. caninum ISCOM ELISA kit. Antibodies to N. caninum were found in 42 of the 236 (17.7%) sera based on ELISA test results. This study is the first report of Neospora infection in this area. With regard to seropositivity, no significant difference was observed regarding origin, sex and age (P > 0.05).

Serological frequency of Neospora caninum in livestock production units in central Veracruz, Mexico

Revista MVZ Córdoba, 2021

Objective. To determine the frequency of Neospora caninum in two Livestock buffalo/bovine Production Units (LPU) in the central zone of the state of Veracruz, México. Materials and methods. The study was a cross-sectional study with non-probability convenience sampling. Two Livestock Production Units (LPUs), both with buffalo and bovine populations, located in the central zone of the state of Veracruz, Mexico, were included. The total populations of buffaloes, bovines, and canines were included. Blood samples were taken between February and March 2019 and processed with a commercial ELISA test (IDEXX ®) and an Indirect Immunofluorescence Test (VMRD, Pullman, WA, USA) to determine Neospora caninum antibodies. In each LPU, a general and individual survey was applied. Data were analyzed with the STATA software version 14.0. Results. 68 out of 138 samples presented antibodies against N. caninum, with a frequency of 45.6% (95% CI, 37.1-54.3). The buffalo population had higher N. caninum antibody concentrations, 26/31 from the LPU of Cotaxtla with 83.8% (95% CI, 66.2-94.5) and 12/22 from the LPU of Medellín with 54.5% (95% CI, 32.2-75.6). Regarding the canines, 4/6 were positive (66.6%). Regarding the different animal species, 38/53 buffaloes presented the highest frequency with 71.6% (95% CI, 57.6-83.2). Conclusions. The presence of N. caninum antibodies in the bovine and buffalo populations of the studied LPUs was verified. There was a higher frequency of antibodies in water buffaloes when they cohabited with cattle and canines.

Detection of antibodies to neospora caninum in moose (alces alces): the first report in europe

Folia Parasitologica, 2014

is a protozoan parasite originally reported as a major cause of bovine abortions worldwide. it is documented that the parasite is widely spread among non-carnivorous cervids. the purpose of this study was to investigate the seroprevalence of N. caninum in moose (Alces alces linnaeus). Blood samples collected in 2010 and 2012 in the northeastern Poland were tested for antibodies to N. caninum by agglutination test (NAt), a commercial competitive screening enzyme-linked immunosorbent assay (cElisA) and enzyme-linked immunoassay (ElisA). sera that gave a positive result were further investigated by western blot (WB) analysis to verify the presence of antibodies. Antibodies to N. caninum were detected in one of seven moose. the antibody titer was confirmed by NAt (1 : 1 280), cElisA (i = 91%) and ElisA (oD = 0.736). the main immunodominant antigens detected by WB were 120, 70, 55, 35 and 16 kDa proteins. this is the first evidence of N. caninum seropositivity in moose living in a natural environment in Europe.

Low seroprevalence of antibodies to Neospora caninum in wild canids in Israel

Veterinary Parasitology, 2006

The role of domestic dogs in the epidemiology of Neospora caninum as well as the relationship between N. caninum infection of farm dogs and cattle were demonstrated, however, evidence is scarce regarding the role of wild canids in domestic animal neosporosis. The present study was undertaken to evaluate the role of wild canids in the epidemiology of bovine neosporosis in Israel by analyzing the prevalence of antibodies to N. caninum in wild canids. Sera samples were collected from 114 free ranging wild golden jackals (Canis aureus), 24 red foxes (Vulpes vulpes) and nine wolves (Canis lupus), which were collected in Israel during the years 1999-2004. Of a total of 147 wild canids tested antibodies to N. caninum were only found in two golden jackals with IFAT titers of 1:50, and in one red fox and one wolf with IFAT titer of 1:400. The low seroprevalence found in this study (2.7%) indicated that wild canids probably do not have an important role in the epidemiology of N. caninum in Israel. However, since the diet of different species of wild canids and even diverse populations of the same canid species vary, it is possible that other results might be obtained from specific wild canids populations, which scavenge in the vicinity of infected bovines.

Seroprevalence of Neospora caninum in non-carnivorous wildlife from Spain

Veterinary Parasitology, 2007

Serum samples from 1034 non-carnivorous wildlife from Spain were tested for antibodies to Neospora caninum by competitive screening enzyme linked immunosorbent assay (ELISA) and confirmed by an indirect fluorescent antibody test (IFAT). High agreement was observed between results in both techniques (kappa value higher than 0.9). Prevalences of N. caninum antibodies positive by both techniques were 11.8% of 237 red deer (Cervus elaphus), 7.7% of 13 barbary sheep (Ammotragus lervia), 6.1% of 33 roe deer (Capreolus capreolus) and 0.3% of 298 wild boar (Sus scrofa). In one of 53 hares (Lepus granatensis), antibodies were found in the ELISA but could not be confirmed by IFAT due to lack of sample. Antibodies to N. caninum were not found in any of 251 wild rabbits (Oryctolagus cuniculus), 79 fallow deer (Dama dama), 27 mouflon (Ovis ammon), 40 chamois (Rupicapra pyrenaica) and three Spanish ibex (Capra pyrenaica). Statistically significant differences were observed between N. caninum seroprevalence in red deer and management of hunting estates (open versus fenced) with higher prevalence in fenced estates, and among sampling sites. Seroprevalence was particularly high in some areas (MO estate in South-Central Spain or some estates of Catalonia, North-East Spain), while no contact with N. caninum was observed in others. Results indicate that in certain areas of Spain, N. caninum is present in wildlife, especially in red deer. These results have important implications in both sylvatic cycles and may influence the prevalence of infection in cattle farms in those areas. To our knowledge, this is the first report of antibodies to N. caninum in wildlife from Spain and the first report of N. caninum antibodies in barbary sheep and wild boar. #

High prevalence of antibodies to Neospora caninum in white-tailed deer (Odocoileus virginianus)

International Journal for Parasitology, 1999

Serum samples of 400 white-tailed deer (Odocoileus virginianus) from 16 preserves in northeastern Illinois were tested for Neospora caninum antibodies in the N. caninum agglutination test using mouse-derived N. caninum tachyzoites and mercaptoethanol. Antibodies were found in 162 deer with titres of 1:40 (47 deer), 1:80 (32 deer), 1:160 (17 deer), 1:200 (eight deer), 1:400 (19 deer), 1:800 (17 deer) and r1:1600 (22 deer). There were no signi®cant dierences in prevalence between age or sex of the deer. The high prevalence of N. caninum infection in deer is consistent with a sylvatic cycle of N. caninum.