Antidiarrhoeal Activity of Methanol Extract of Leaves of Calotropis procera R. Br (original) (raw)
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Studies on anti-diarrhoeal activity of Calotropis gigantea R.Br. in experimental animals
Journal of pharmacy & pharmaceutical sciences : a publication of the Canadian Society for Pharmaceutical Sciences, Société canadienne des sciences pharmaceutiques, 2004
Calotropis gigantea R. Br. (Asclepiadaceae) a wildly growing plant has been reported to possess number of medicinal properties and other purposes. The purpose of the present study was to evaluate scientifically the anti-diarrheal effects of C. gigantea used traditionally in Indian system of medicine using castor oil-induced diarrhoea model. The anti-diarrheal effect of hydroalcoholic (50:50) extract of aerial part of Calotropis gigantea was studied against castor oil-induced-diarrhea model in rats. The gastrointestinal transit rate was expressed as the percentage of the longest distance traversed by the charcoal divided by the total length of the small intestine. The weight and volume of intestinal content induced by castor oil were studied by enteropooling method. Like atropine (3 mg/kg, i.p.) there were significant reductions in fecal out put and frequency of droppings when the plant extracts of 200 and 400 mg/kg doses were administered intraperitoneally compared with castor oil t...
Anti-diarrhoeal activity of the latex of Calotropis procera
Journal of Ethnopharmacology, 2001
The dry latex (DL) of Calotropis procera (Asclepiadaceae), a potent anti-inflammatory agent has been evaluated for anti-diarrhoeal activity. Like atropine and phenylbutazone (PBZ), a single oral dose of DL (500 mg/kg) produced a significant decrease in frequency of defecation, severity of diarrhoea and afforded protection from diarrhoea in 80% rats treated with castor oil. To understand the mechanism of its anti-diarrhoeal activity, we further evaluated its effect on intestinal transit, castor oil induced intestinal fluid accumulation (enteropooling) and electrolyte concentration in the intestinal fluid. DL produced a decrease in intestinal transit (27-37%) as compared to both normal and castor oil treated animals. Unlike atropine, DL significantly inhibited castor oil induced enteropooling. However, it did not alter the electrolyte concentration in the intestinal fluid as compared to castor oil treated rats.
2005
The leaves of Calotropis procera R.Br was undertaken for evaluation of antidiarrhoeal activity in Albino mice (male) weighing between 40-50gm was used in this study. The selected and authenticated plant extract was prepared by maceration with using a methanol as a solvent for 7 days. From the toxicity study; it was observed that plant extract is non toxic and caused no death up to the dose of 500 mg/kg. It is safe and used for further experiment. The methanol extract of Calotropis procera showed significant activity against castor oil induced diarrhoea to compared standard drug Loperamide in albino mice. All the values obtained in the studies were expressed as mean ± SEM. by one way of analysis of variance for determining the significant difference. On the basis of these findings, it can be assumed that Calotropis procera R.Br could be a potential source for novel "lead" discovery for antidiarrhoeal drug development. Further study is necessary to evaluate the active principle responsible for the antidiarrhoeal activity and clear mechanism of action involved.
Different partitionates of leaves of Calotropis gigantea were evaluated for membrane stabilizing activity by reduction of hemolysis in hypotonic solution and heated solution along with cytotoxic and anti-diarrheal activities. The first activity was assessed by using erythrocyte in hypotonic solution and heat in which acetyl salicylic acid, the reference standard, gave 71.9 ± 0.004% and 42.2 ± 0.005% inhibition of hemolysis in both conditions, respectively. Among the partitionates of the leave extracts maximum membrane stabilizing activity was revealed by aqueous soluble fraction (63.77 ± 0.022%) in hypotonic solution and petroleum ether soluble fraction (32.62 ± 0.052%) in heat induced condition. In determining cytotoxic activity using brine shrimp nauplii, crude methanol extract showed the most cytotoxicity (LC50 value of 1.79 ± 0.004 μg/ml) among the partitionates whereas the standard vincristine sulphate gave LC50 value of 0.45 ± 0.008 μg/ml. The anti-diarrheal activity of the crude methanol extract was determined on mice using loperamide as standard. The extract exhibited significant anti-diarrheal activity at both doses (200 mg/kg and 400 mg/kg). Key words: Calotropis gigantea, Membrane stabilizing activity, Cytotoxic activity, Anti-diarrheal activity, Acetyl salicylic acid, Loperamide, Vincristine sulphate.
Antidiarrheal Activity of Three Medicinal Plants in Swiss Albino Mice
International Journal of Biomedicine, 2016
Background: Different parts of Allamanda neriifolia (AN), Crinum latifolium (CL), and Bruguiera cylindrica (BC) are used in folk medicine to treat diarrhea. Therefore, the aim of this study was to investigate and compare possible antidiarrheal activity of the crude extracts from barks, stems, and roots of AL, CL, and BC in Swiss albino mice. Methods: Antidiarrheal activities of extracts were evaluated at three doses (100 mg/kg, 200 mg/kg and 400 mg/kg) and compared with Loperamide in a castor oil-induced diarrhea and charcoal meal test model in the Swiss albino mice. Results: The aqueous extract of CL and BC administered at doses of 100, 200 and 400 mg/kg showed 0%, 24.5%, 62.26% and 5.66%, 37.11%, and 62.26% diarrhea inhibition, respectively. This reduction in diarrheal episodes is significant, and maximum effect was observed at the dose of 400 mg/kg similarly in the alcohol extracts of both CL and BC. AN administered at the dose of 100, 200 and 400 mg/kg showed 55.97%, 74.84% and 74.84% diarrhea inhibition, respectively. The aqueous extracts of AN, CL and BC were able to increase the percentage inhibition of the charcoal meal movement. Conclusion: The antidiarrheal effect of the AN extract, in contrast to CL and BC, against the castor oil-induced diarrhea model prove its efficacy in an extensive range of diarrheal conditions.
Calotropis procera: A Phytochemical and Pharmacological Review
2016
Medicinal plants are used from the ancient time as the major sources of drugs.The fact is that we can obtain many of the presently available drugs, either directly in the extract form or in the modified synthetic form. Naturally, plants have ability to synthesize products benificial for us namly as phytoconstituents that are used to perfom biological functions, which also protect us against predators like virus fungi and other microorganisms. The phyto-constituents obtained from the natural products are one of the most successful strategies for the discovery of new drugs. Various research are emphasised on scientific evaluation of herbal drugs which obtained from plant for the treatment of various ailments. Calotropis procera is small, erect shrub, which is used in several herbal and empirical medicines to cure simple and deadly diseases and disorders. This shrub is known to possess wide range of pharmacological activity. Calotropis procera is also reported widely in various folklor...
In Vivo Study of Pharmacological Activities of Calotropis Procera: An Overview
International Journal of Research in Ayurveda and Pharmacy
Calotropis is one of the widely distributed plants along the world’s geographical area. Many studies have been conducted to prove the pharmacological activities of Calotropis procera (Arka). It is classified as Upavisha in Ayurveda by Bhavprakasha and Rasatarangini. In its therapeutic application mentioned in various classical texts, Calotropis procera is treating almost 58 diseases having its 547 formulations which are indicated. It contains many phytochemical constituents like Cardenolides, Benzoyllineolone, Calactin, Calotropagenin etc. These phytoconstituents have proven anti-inflammatory, anticonvulsant, hepatoprotective, antitumor, and cytotoxic properties. In this review, a thorough literature search has been done through various textbooks, research magazines and internet search engines like PubMed, Google Scholar etc. Different In Vivo studies carried out on Calotropis procera to assess its pharmacological activities are considered. Meticulous analysis of these studies has b...
A review on phytochemical constituents and pharmacological potential ofCalotropis procera
RSC Advances, 2021
Calotropis procera is locally known as Aak or Madar in Hindi, milk weed in English and belongs to the family Apocynaceae and subfamily Asclepiadoideae. Although a wasteland plant, it is of sacred use as its flowers are offered for worshipping Lord Shiva, a Hindu God. Tribes all over the world use the plant in treatment of various diseases like snake bite, body pain, asthma, epilepsy, cancer, sexual disorders, skin diseases and many more. This plant contains various phytoconstituents such as flavonoids, terpenoids, cardenolides, steroids oxypregnanes etc. Though literature searches reveal many reviews about ethnomedicinal uses, chemical composition and pharmacological activities, no recent papers are available that provide an overview of the therapeutic potential and toxicity of Calotropis procera. Hence, the insight of this review is to provide a systemic summary of phytochemistry, pharmacology, toxicology and therapeutic potential of Calotropis procera and to highlight the gaps in the knowledge so as to offer inspiration for future research.