Effect of Bifidobacterium longum ingestion on experimental salmonellosis in mice (original) (raw)
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Archives of …, 2010
The aim of the present study was to compare the effect of intragastric administration with two strains of Bifidobacterium animalis subsp. lactis (Bifido A and Bifido B), in gnotobiotic and conventional mice, challenged with Salmonella Typhimurium. In vitro antagonism test showed that the two strains were able to produce antagonistic substances against various pathogenic microorganisms. In an ex vivo antagonism test the production of antagonistic substances was observed only against three out ten pathogens tested. Both Bifidobacterium strains were able to colonize and to maintain high population levels in the digestive tract of gnotobiotic mice. In addition, the two strains had low and limited translocation ability and did not cause any histological lesion in any of the organs analyzed. Both strains were able to reduce the fecal number of Salmonella in gnotobiotic mice challenged with the pathogen, but only Bifido B was able to confer a protection as demonstrated by a lower mortality. Higher levels of sIgA and IL-10 were observed only in Bifido B mono-associated mice when compared to germ free group. We could conclude that, among the parameters analyzed, the strain Bifido B exhibited the more desirable characteristics to be used as a probiotic.
IMMUNOMODULATORY ACTIVITY OF PROBIOTICS AGAINST SALMONELLA TYPHIMURIUM INFECTED MICE
INTERNATIONAL SCIENTIFIC CONGRESS "MEDICAL AND BIOLOGICAL SCIENCES: ACHIEVEMENTS AND PERSPECTIVES" :25-26 April 2018.40-47, 2018
In this study Wistar albino rats were chosen to evaluate the effectiveness of probiotic therapy. They were induced with infection using known bacterial pathogen Salmonella typhimurium. They were treated separately with common probiotics such as Lactobacillus acidophilus, Streptococcus faecalis, Bacillus megaterium and mixed culture of probiotics. The results of immunomodulatory activities have been found. The reason behind that may be the ways and means how the organisms accommodate and accustom to the intestinal environment and the production of various metabolites attributed to be a protective agents. Being immunomodulatory agent and nontoxic in nature have made these agents more useful as therapeutic agents or as supplements.
Indonesian Journal of …, 2010
Probiotic is defined as live microorganism added to food that give benefit to host by increasing the microbial balance of intestinal (Ziemer et al., 1998). Another definition stated that probiotic is live microorganism which is consumed by oral and give health effect (Losada et al., 2002; Champagne et al., 2005). Some lactic acid bacteria (LAB) have been studied as potent probiotic. Current researchs of probiotic focus on the capability of the products to increase immune system and immune response mechanism which is thought to play an important role to protect the body from any
Brazilian Archives of Biology and Technology, 2012
The probiotic influence on the immune system, especially under pathogenic challenge conditions, still remains controversial. To address this, a systematic review of current studies concerning the efficacy of probiotics on the immune response of rats subjected to experimental challenges was conducted. The survey was conducted using PubMed, ISI Web of Science and Scielo databases. Only studies which tested probiotics in vivo in rats were included. The experimental design, methodological quality, and results of the articles were analyzed. In total 21 articles were selected for this study. The most commonly used microorganisms in the experiments were those of the genus Lactobacillus, which was reported in 12 articles. The second most often used genus was Bifidobacterium (B. animalis and B.longum). In general, the probiotics use against experimental pathogenic challenges was successful: 86% of the selected articles reported a beneficial effect on the immune response associated with the use of probiotics.
Archives of Microbiology, 2010
The aim of the present study was to compare the effect of intragastric administration with two strains of Bifidobacterium animalis subsp. lactis (Bifido A and Bifido B), in gnotobiotic and conventional mice, challenged with Salmonella Typhimurium. In vitro antagonism test showed that the two strains were able to produce antagonistic substances against various pathogenic microorganisms. In an ex vivo antagonism test the production of antagonistic substances was observed only against three out ten pathogens tested. Both Bifidobacterium strains were able to colonize and to maintain high population levels in the digestive tract of gnotobiotic mice. In addition, the two strains had low and limited translocation ability and did not cause any histological lesion in any of the organs analyzed. Both strains were able to reduce the fecal number of Salmonella in gnotobiotic mice challenged with the pathogen, but only Bifido B was able to confer a protection as demonstrated by a lower mortality. Higher levels of sIgA and IL-10 were observed only in Bifido B mono-associated mice when compared to germ free group. We could conclude that, among the parameters analyzed, the strain Bifido B exhibited the more desirable characteristics to be used as a probiotic.
Beneficial microbes, 2014
The mouse has been largely used for the study of the protective capacity of probiotics against intestinal infections caused by Salmonella. In this work we aimed at comparing the mortality and translocation assay for the study of the protective capacity of the human breast milk-derived strain Bifidobacterium animalis subsp. lactis INL1 on a model of gut infection by Salmonella enterica subsp. enterica serovar Typhimurium. Different doses of S. Typhimurium FUNED and B. animalis subsp. lactis INL 1 were administered to Balb/c mice in a mortality or a translocation assay. The survival of the control group in the mortality assay resulted to be variable along experiments, and then we preferred to use a translocation assay where the preventive administration of 109 cfu of bifidobacteria/mouse for 10 consecutive days significantly reduced the number of infected animals and the levels of translocation to liver and spleen, with enhanced secretory immunoglobulin A and interleukin 10 production...
Scientific Reports, 2020
Salmonella infections have become a major health concern in recent decades. This pathogen has evolved to become resistant to antibiotics, which has caused problems in its treatment. As such, finding a novel preventive method is important in the treatment and management of this infection. In recent years, uses of probiotics, especially spore-former genera such as Bacillus spp. has become increasingly popular. In this study spores of two probiotic bacteria, Bacillus subtilis and Bacillus coagulans were fed to rats for three weeks through their daily water intake after which Salmonella Typhimurium was gavaged to the rats. On days 1, 3, 5 and 7 after gavaging, the number of Salmonella was counted in liver, spleen, mesenteric lymph nodes, feces and content of ileum and cecum. Hematological and biochemical parameters, inflammatory mediators, total antioxidant capacity and malondialdehyde were also measured. The results showed that B. subtilis and B. coagulans caused delation in infiltration of Salmonella into the lymph nodes, spleen and liver, reduction of the inflammatory mediators, and decreases in oxidative stress, hematological and biochemical changes. The overall count of Salmonella in the above mentioned parameters has also decreased and a faster return to normal base were also witnessed. The results showed that the use of B. subtilis and B. coagulans can potentially help boost the body's immune system, to combat the effects of exposure to the Salmonella pathogen. Salmonella infections are considered to be a serious and harmful infection affecting humans and animals and a great concern for human health 1. Direct contact with infected animals or their products makes humans infected. Among Salmonella spp., Salmonella enterica serovar Enteritidis (S. Enteritidis) and Salmonella enterica serovar Typhimurium (S. Typhimurium) are the main serovars that cause human salmonellosis 2. Salmonellosis causes a lot of costs, which includes treatment costs, productivity reduction, the value of premature death and also economic losses because of the livestock and poultry death 3. Preventative measures such as usage of probiotics in animals can control and reduce Salmonella infection, and its costs 4,5. Probiotics are live microorganisms, which have health benefits if administered in adequate amounts 6,7. Most of the reported research on probiotics have focused on the various strains of Lactobacillus. In recent years, uses of spore-forming bacteria and especially Bacillus spp., as probiotics have increased 8. The bacteria's ability to form spore results in higher resistance during the production and storage processes, as well as gastric (pH, digestive enzymes) and intestinal environmental conditions 9. Through direct contact with epithelial cells, also immune cells, activation of gut microflora and modification of its composition, innate and adaptive immunity are influenced by probiotics 10. Modulation of pro-inflammatory (like IFN-γ and TNF-α) and anti-inflammatory cytokines (IL-10) is one of the principal mechanisms that probiotic influence the immune system 11. Many studies have investigated the effect of spore-forming probiotics such as Bacillus spp. and non spore-forming probiotics such as Lactobacilli on Salmonella infection and most found that probiotics reduce the
Comparative effects of six probiotic strains on immune function in vitro
The British journal of nutrition, 2012
There is considerable interest in the strain specificity of immune modulation by probiotics. The present study compared the immunomodulatory properties of six probiotic strains of different species and two genera in a human peripheral blood mononuclear cell (PBMC) model in vitro. Live cells of lactobacilli (Lactobacillus casei Shirota, L. rhamnosus GG, L. plantarum NCIMB 8826 and L. reuteri NCIMB 11951) and bifidobacteria (Bifidobacterium longum SP 07/3 and B. bifidum MF 20/5) were individually incubated with PBMC from seven healthy subjects for 24 h. Probiotic strains increased the proportion of CD69+ on lymphocytes, T cells, T cell subsets and natural killer (NK) cells, and increased the proportion of CD25+, mainly on lymphocytes and NK cells. The effects on activation marker expression did not appear to be strain specific. NK cell activity was significantly increased by all six strains, without any significant difference between strains. Probiotic strains increased production of ...
International Journal of Food Microbiology, 2010
The prevention of pathogen infections is one of the most extensively studied effects of probiotics. L. casei CRL 431 is a probiotic bacterium and its effects on the gut immune cells have been extensively studied. The aim of the present study was to determine, using a mouse model, the preventive and therapeutic effect of L. casei CRL 431 to achieve protection against Salmonella enteritidis serovar Typhimurium infection. In both previous and continuous (previous and post-infection) probiotic administration, the mechanisms induced by this lactic acid bacteria on the first line of intestinal defense (non-specific barrier and the innate immune cells associated to the gut), as a way to understand some of the mechanisms involved in the protection against Salmonella enteritidis serovar Typhimurium, were analyzed. The results obtained demonstrated that 7 days L. casei CRL 431 administration before infection decreased the severity of the infection with Salmonella enteritidis serovar Typhimurium, demonstrating that the continuous administration (even after infection) had the best effect. This continuous administration diminished the counts of the pathogen in the intestine as well as its spread outside this organ. Several mechanisms and cells are involved in this protective effect against Salmonella enteritidis serovar Typhimurium. L. casei CRL 431 acted on cells of the innate and adaptive immune response. The probiotic administration decreased the neutrophil infiltration with the consequent diminution of intestinal inflammation; activated the macrophage phagocytic activity in different sites such as Peyer's patches, spleen and peritoneum; and increased the number of IgA + cells in the lamina propria of the small intestine which was correlated with increased release of s-IgA specific against the pathogen in the intestinal fluids. The mechanism of the inhibition of cellular apoptosis was not involved.