Analysis of Total Flavonoids in Herbal Drugs Expressed as Quercetin by Reversed Phase-UHPLC Method (original) (raw)
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A new and rapid HPLC method for identification and determination of myricetin, quercetin, kaempferol and total flavonoids in ten herbal drugs of Macedonian origin is presented. Preparation of samples (Uvae ursi folim, Pruni spinosae flos, Sambuci flos, Betulae folim, Primulae flos, Herniariae herba, Centaurii herba, Tiliae flos, Robiniae pseudoacaciae flos, Bursae pastoris herba) included hydrolysis of glycosides and extraction of total aglycones with ethyl acetate. HPLC analysis with UV-diode array detection was carried out on RP C18 column, using 5% acetic acid and acetonitrile in agradient elution mode and column temperature of 30 o C. The monitoring of the elution is performed in the whole UV-range and the acquisition of data for quantitative analysis at 367 nm. Screening of the extracts showed presence of quercetin in nine, kaempferol in seven and myricetin in only one sample. The quantitative analysis showed that the content of quercetin ranged from 0.026-0.506 % (m/m), while for kaempferol it was from traces to 1.246 %. Uvaeursi folium and Pruni spinosae flos were rich in content of quercetin (0.482 % and 0.506 %, respectively), while Pruni spinosae flos and Robiniae pseudoaccaciae flos contained the highest amounts of kaempferol (1.246 % and 0.892 %, respectively). Myricetin was identified and determined only in Betulae folium (0.102 %). The content of total flavonoids in the investigated samples expressed in terms of quercetin ranged from 0.040 to 1.680 %. The proposed HPLC method is convenient for use in routine analysis of myricetin, quercetin and kaempferol, as well as for estimation of total flavonoids content in herbal drugs.
Determination of quercetin in dietary supplements by isocratic RP-HPLC method
2021
Quercetin is a natural flavonoid found abundantly in vegetables and fruits. It shows antioxidant, anti-inflammatory, antihepatotoxic, antiallergic, antidiabetic and antiviral activity. Increasingly popular dietary supplements containing quercetin require critical examination of their quality. The aim of this study was to develop a simple and accurate HPLC method for quercetin determination in dietary supplements. Chromatographic separation was achieved by isocratic method, using a Purospher STAR RP-18 reversephase column (150 x 4.6 mm i.d., particle size 5 μm), a mobile phase composed of acetonitrile and water (acidified to pH 3.0), in a ratio of 30:70 (V/V), run at a flow rate of 1.1 mL/min. The column temperature was kept at 30 °C. The DAD detector was set at 257 nm and 375 nm. The injection volume was 20 μL. Isopropanol was used as a solvent. The method was validated by determining system suitability, specificity, linearity, range, limit of detection and quantification, accuracy,...
A Simple HPLC Method for Quantitation of Quercetin in Herbal Extracts
Journal of AOAC INTERNATIONAL
A reversed-phase HPLC method with UV detection was developed for the determination of quercetin. The method produced linear response over a wide concentration range, with an average accuracy of 95% and average intra- and interday variation of 0.75 and 0.3, respectively. The exactness of the method was proven by determining the recovery rates from 50 to 150% of standard concentration, which were found within the acceptable range of 95 to 105%. The method was used for quantitation of quercetin in the extracts of Psidium guajava, Vitis vinifera, and extracts rich in quercetin and other flavonols in the flavonoid family.
Journal of Liquid Chromatography & Related Technologies, 2001
of Maced on ia , Institute of Pharmac ogno sy, Faculty of Pharmacy, Vod nj an ska 17, 1000 Skopje, Repub lic of Maced on ia ABSTRACT A new and ra pid procedure for sc reening of flavonols (rnyrice tin, querce tin and kaernpferol) and fo r determ ination o f quer cetin by RP-I-IP LC w ith UV-di ode array detecti on in 16 medi c inal plants is presen ted . Scree ning of the ex trac ts sh owed that q uerce tin is the most abundan t flavon ol , es pecia lly in Hyp erici herba , Uvae ursifolium and Pruni sp inosaejlos . Kaemp ferol was the most ab unda nt in Rvbi niae pse udoaca ciae flos an d Pruni spin osaeflos, whe reas myricetin was identified onl y in Betulae folium.
2021
Herbal medicines and their preparations have been mostly used since from thousands of years in all developing and developed countries in the primary health care of society and community. Flavonoids are the class of polyphenolic compounds, which are mainly distributed throughout the plant kingdom. Quercetin is a flavonoid which shows major pharmacological activities and effectively used for the management and treatment of various diseases and disorders. Many herbal medicines and their formulations containing Quercetin are available in market and hence quality control of Quercetin in is very important and essential in manufacturing industries We have reviewed various scientific research published on quality control analysis and standardization of Quercetin in its isolated form, extract or any other herbal or polyherbal preparation. We have mainly focused on the spectroscopic and chromatographic methods for qualitative and quantitative analysis of Quercetin and they were comprehensivel...
HPLC-DAD method for simultaneous determination of natural polyphenols
HPLC-DAD method for simultaneous determination of natural polyphenols
A simple and reliable high-performance liquid chromatography with diode-array detection (HPLC-DAD) method was developed for simultaneous determination of 9 natural substances common in plants: three major catechins ((-)-epicatechin gallate, (-)-catechin, (-)-epigallocatechin), four major fl avonoids (rutin, quercetin, myricetin, kaempferol), gallic and vanillic acid. The optimized method was carried out for 40 minutes with detection wavelengths of 278 and 368 nm, gradient elution system on a C18 reversedphase column. The developed system was evaluated for several validation characteristics, as system suitability, specifi city, linearity, limit of detections (LODs) and limit of quantifi cations (LOQs). The newly established HPLC method was proved to be specifi c, sensitive, linear and precise. The received results showed good chromatographic separation and assumed that the described method was validated. The HPLC-DAD method can be a useful tool for the quantitative and qualitative evaluation of the selected polyphenol compounds and can be improved in the future for the examination of the polyphenols of interests in individual herbal infusions.
Development and validation of HPLC method for determination of flavonoids in herbal preparations
2015
Alogliptin benzoate, a member of dipeptidyl peptidase-4 inhibitors, is a recent drug developed by Takeda Pharmaceutical Company for the treatment of Type 2 diabetes; it potentiates the effect of incretin hormones through the inhibition of their degradation. Alogliptin can be used alone or in combination therapy. A new sensitive and rapid HPLC method was developed for the determination of alogliptin benzoate in bulk and pharmaceutical dosage forms; it was validated according to ICH and FDA guidelines. e HPLC analysis was performed on the Agilent 1200 system equipped with a Hypersil Gold ermo Scientific C18 (250 cm × 4.6 mm) 5 µm column, with a mixture of acetonitrile and ammonium carbonate buffer in the ratio of 55 : 45 v/v as the mobile phase, at the flow rate of 1.0 mL/min. e detection was performed at the wavelength (λ) of 277, and the retention time of alogliptin benzoate was around 4 min. e total run time was 6.0 min. e calibration plot gave linear relationship over the concentration range of 85-306 µg/ml. e LOD and LOQ were 0.03 and 0.09 μg, respectively. e accuracy of the proposed method was determined by recovery studies and was found to be 100.3%. e repeatability testing for both standard and sample solutions showed that the method is precise within the acceptable limits. RSD% of the determination of precision was <2%. e results of robustness and solutions stability studies were within the acceptable limits as well. e proposed method showed excellent linearity, accuracy, precision, specificity, robustness, LOD, LOQ, and system suitability results within the acceptance criteria. In addition, the main features of the developed method are low run time and retention time around 4 min.
Development and Validation of a New RP-HPLC Method for Determination of Quercetin in Green Tea
Журнал аналитической химии, 2013
ABSTRACT 906 1 Green tea (Camellia sinensis) originates from Chii na and has anticancer, antiinflammatory and antioxii dative activity. Green tea has attracted significant att tention for its health benefits in a variety of disorders, ranging from cancer to weight loss [1]. Tea is the most consumed drink in the world after water [2]. A plant extract of tea contains quercetin as a bioo active compound [3, 4]. The estimated amounts of quercetin are in the range of 2–2.5 g/kg of the plant material. Otherwise, a quercetin (3,3&amp;amp;#39;,4&amp;amp;#39;,5,77penn tahydroxyflavone) belongs to a group of polyphee nols and is the most abundant dietary flavonoid [5 ⎯ 8]. The structural elements of quercetin are three rings and five hydroxyl groups, which are the building blocks for other flavonoids. Several chromatographic techniques have been employed to separate, characterize, and quantify indii vidual polyphenols in teas [9]. The different methods for monitoring the quercetin concentration in the green tea extract were described in the literature. Wang et al. [10] optimized the conditions for hydrolyzing and determining flavonols in tea leaves and tea infuu sions in an isocratic elution HPLC system. The two high resolution, gradient elution reverseephased HPLC systems for the separation of over 30 flavonols, flavann33ols, and related compounds in green and black teas and their identification by diode array dee tection and electrospray mass spectrometry (MS) uss
Journal of Chromatography B: Biomedical Sciences and Applications, 1995
A method is reported for the measurement of quercetin in human plasma using reversed-phase high-performance liquid chromatography (HPLC). Quercetin and kaempferol (as internal standard) were spiked into plasma samples and extracted using ClS Sep-Pak Light cartridges (efficiency > 85%). Flavonoids were eluted with aqueous acetone (50% v/v, pH 3.5), dried down and redissolved in aqueous acetone (45% v/v, pH 3.5). The increased osmolarity promoted a phase separation and the water-saturated acetone layer, containing the flavonoids, was analysed by HPLC with aqueous acetone mobile phase (45% v/v acetone in 250 mM sodium dihydrogen sulphate. The mixture was adjusted to pH 3.5 with phosphoric acid and used at a flow-rate of 1.0 ml/min) and/~Bondapak C18 column (150 × 3.9 mm I.D., 10/xm particle size). The detection limit (A375 nrn) for quercetin in plasma was 0.1 ~g/ml (300 nM). The method also detects metabolites of quercetin, although these are not yet identified. * Corresponding author. 0378-4347/95/$09.50 ~) 1995 Elsevier Science B.V. All rights SSDI 0378-4347(94)00549-4
Journal of medicinal plant research
Murraya koenigii L. Spreng, a medicinally important herb of Indian origin, has been used for centuries in the Ayurvedic system of traditional Indian medicine and popularly used in Indian cuisine on a daily basis. To evaluate the quality of M. koenigii, L. leaves, a sensitive, simple and precise reversed-phase high performance liquid chromatography (RP-HPLC) method was developed for assessment of four major bioactive flavonoids: rutin, quercetin, myricetin and kaempferol. Separation was achieved on a reversed phase column (ZORBAX, Eclipse plus-C18, 5 μm, 4.6 × 150 mm, Agilent, USA) and methanol:acetonitrile:water:acetic acid (40:20:39:1, v/v/v/v) was employed as the eluent. Sample was eluted at 0.8 ml/min and peaks were simultaneously identified using UV absorbance at 350 nm for kaempferol and 254 nm for rutin, myrcetin, and quercetin. The authenticated four analytes were used to construct linear standard curves by injecting range of 20 to 200 ng. The correlation coefficients of the ...