Uses of single dose dependent and relative potency assays for the evaluation of inactivated fowl cholera vaccine (original) (raw)
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Journal of World's Poultry Research
Fowl cholera is a septicemic respiratory complex caused by Pasteurella multocida, widely distributed in poultry and other avian species and of major economic importance. A total of 37 different inactivated Pasteurella multocida vaccines from different sources either locally prepared or imported from different sources were comparatively tested for relative potency following both single dose and booster dose vaccination assays. The study objective was to minimize the time factor exhausted in the evaluation processes of the inactivated fowl cholera vaccines. So it is planned to compare between single and booster dose vaccinations and their related potency. Correlation between protection associated with the single dose and booster dose vaccination were evaluated and average requirement for protection was 43.7% in single dose vaccination assay compared to 76.2 % associated with booster dose vaccination assay. In the same concern, the correlation between both assays for the seroconversion was estimated using ELISA and the minimum requirement was 1.8× cut off value in the single dose vaccination assay compared to 2.25× cut off value in the booster dose vaccination assay. In conclusion, single dose vaccination assay could be valuable in the evaluation of inactivated fowl cholera vaccines through determination of protection indices and/or estimation of humoral immune response if the above mentioned data is considered.
Efficacy of Inactivated Fowl Cholera Vaccine in Chickens
2018
Fowl cholera is highly prevalent bacterial disease in poultry population of India. Several areas of India have been reported with outbreaks of fowl cholera. Fowl cholera severely affects the health of flocks, causes morbidity and mortality. Drop in egg production with worsened egg quality also results due to poor health of flock. The disease is also prevalent in village chicken and vaccination is the effective tool to control the prevalence of the disease. In present research, 30 specific pathogen free birds were divided into vaccinated (Group 1; 20 birds) and control (Group 2; 10 birds) groups. Birds from group 1 were vaccinated with inactivated fowl cholera vaccine and 14 days later given booster dose. After 21 days of booster dose, both groups were challenged with virulent strain of fowl cholera disease. At 7 days interval blood was collected and serology was performed for presence of antibodies against the disease with ELISA technique. The vaccine was found effective with respec...
In Process Quality Control Factors Affecting Potency of Fowl Cholera Vaccine
International Journal of Biology
Fowl cholera (FC) is one of the respiratory syndromes of commercial layer, breeder and broiler farms in Pakistan. The disease is mainly controlled by vaccination. In the present study, effect of “in process quality control” factors such as amount of immunogen, chemical nature of adjuvant, fractions of bacterial culture and its storage on potency of the vaccine was investigated. Immunogen amount (1011CFU/ml) induced serum anti-Pasteurella multocida ELISA (anti-PM-ELISA) antibody titer in the vaccinated birds on 32 days post vaccination that was significantly higher (P<0.05) than that of 109 and 1010CFU/ml. Montanide based whole culture vaccine induced better antibody response as compare to aluminum hydroxide gel. Washed culture of P. multocida (bacterial sediment) induced significantly higher anti-PM-ELISA antibody titer as compared to the vaccine prepared from purified LPS or whole culture vaccine (p<0.05). Storage of oil based FC vaccine at refrigerated temperatur...
Development of washed cell fowl cholera vaccine in Bangladesh
2004
An experiment was conducted to develop washed cell fowl cholera (WCFC) vaccine with virulent avian Pasteurella multocida (PM 38) serotype 1 (X-73). A total of 20 Fayoumi birds of either sex of 10 weeks aged were divided into two groups as group A (immunized with washed cell fowl cholera vaccine) and group B (unvaccinated control). Primary vaccination was given through IM route in each birds of group A and booster dose was given through SC route after 15 days of primary vaccination. The presence of antibody against P. multocida was determined by slide agglutination test (SAT) and growth inhibition test (GIT). The degree of antibody levels of prevaccination and post vaccination sera were determined by passive haemagglutination assay (PHA). Sera mean PHA titres at 15, 21, 28 and 42 days post-vaccination in group A were 30.4±4.43, 46.4±6.06, 67.2±11.14 and 134.4±22.28 respectively. The present results revealed that WCFC vaccine worked satisfactory in terms of protection rate against Avian Pasteurellosis. It was also demonstrated that experimental WCFC vaccine conferred 80% protection against challenge infection when all chickens of control group failed to survive against challenge infection.
Comparative efficacy of BAU-fowl cholera and DLS-fowl cholera vaccines in indigenous chicken
Research in Agriculture Livestock and Fisheries
The present study was conducted to determine the immune response induced in indigenous chicken produced against BAU-FC and DLS-FC vaccines with their efficacy study against Pasteurella multocida. A total of forty (40) chickens were selected and divided into Group A (15), Group B (15) and Group C (10). Group A and B were vaccinated with BAU-FCV and DLS-FCV, respectively at the dose rate of 0.5 ml through SC at six weeks of age followed by boostering at 10 weeks of age while Group C was kept as unvaccinated control. Sera samples were collected after primary and booster vaccination and antibody titre was determined by Passive hemagglutination (PHA) test. The mean PHA titres recorded at 4 weeks after primary vaccination was 51.20 ± 7.84 in birds of group A and 38.40 ± 6.40 in birds of Group B. After booster vaccination, mean PHA titer was found 140.80 ± 31.35 at 16 weeks of age in case of BAU-FC vaccinated group and 115.20 ± 12.80 in case of DLS-FC vaccinated group. The mean PHA titer w...
Standardization of Effective Dose of Fowl Cholera Vaccine in Pigeon in Bangladesh
Bangladesh Journal of Veterinary Medicine, 2018
An experiment was conducted to determine the effective dose of formalin killed (FK) fowl cholera (FC) vaccines prepared with virulent avian Pasteurella multocida (PM 38) serotype 1 (X-73) collected from the laboratory of the Department of Microbiology and Hygiene, BAU, Mymensingh. To determine the effective dose of vaccine, 7 weeks old 30 pigeons were immunized and each group consists of 5 birds. The groups are represented by A, B, C, D, E and F. The birds belonging to groups (A-E) were vaccinated with different doses of vaccine, after two weeks of first, second immunization and challenge experiment, blood was collected from all vaccinated birds, and serum was analyzed to determine antibody titer against P. multocida by passive hemagglutination test (PHA). The PHA titer after two weeks of first vaccination were 16±3.92, 17.6±3.92, 25.6±3.92, 32±8.76, 35.2±7.84 of group A,B,C,D and E, respectively at the dose of 0.2ml (0.26×10 8 CFU)/birds, 0.4ml (0.5×10 8 CFU)/birds, 0.8 ml (1.04×10 8 CFU)/birds, 1ml (1.3×10 8 CFU)/birds, respectively. The PHA titer of prevaccination and control birds was <4. The PHA titer after 2 weeks of second vaccination or boostering were 32±8.76,
Comparative efficacy of fowl cholera vaccines in ducks
2015
An experiment was conducted to determine the comparative efficacy of fowl cholera vaccines in ducks prepared at BAU, (FCV-BAU) Mymensingh with that of FC vaccine prepared at Livestock Research Institute, (FCV-LRI), Dhaka, using SC route of inoculation. For this 22 weeks aged ducks of Xinding breed was divided into three groups such as A, B, and C, of which group A was inoculated with FCV-BAU and group B with FCV-LRI, while group C was kept as unvaccinated control. Each bird received initially 1 ml of Fowl cholera vaccine administered SC followed by a booster dose given with the similar dose and route at 15 days interval. The birds were reared separately in duck shed of the department with recommended feed and other managemental requirements as well as maintenance of proper biosecurity. Pre-vaccination sera were collected from all the groups of ducks. Sera of the immunized and control ducks were collected at 15 days of primary vaccination and then 28, 35, 42, 49 and 56 days post vacc...
Comparative immunogenicity of fowl cholera vaccine in Jinding ducks
Bangladesh Veterinarian, 2014
This study compared the immunogenicity of alum-precipitated formalin-killed fowl cholera vaccines (BAU-FCV and LRI-FCV) in Jinding ducks. The ducks were divided into three groups (A = 14, B = 14, C = 12). Group A was inoculated with BAU- FCV 0.5 mL and group B with LRI- FCV 1.0 mL intramuscularly (im) at the age of six weeks and group C served as unvaccinated control. Booster vaccination was administered similarly at 11 weeks of age in groups A and B. Challenge infection was given to all birds two weeks after booster vaccination. Passive Haemagglutination Assay (PHA) antibody titres in group A were 59.4 ± 4.6 21 days after primary vaccination, 137.1 ± 21.8 15 days after booster vaccination, 100.6 ± 12.9 21 days after booster vaccination, and 256.0 ± 48.4 15 days after challenge. In group B, titres were 50.3 ± 6.5, 118.9 ± 9.1, 91.4 ± 12.9, 237.7 ± 51.7, respectively, whereas titres in group C remained at ?4.0 ± 0.0. The antibody titres were insignificant when compared between pre-va...
2019
Fowl cholera caused by Pasteurella multocida is among the serious infectious diseases of poultry in Ethiopia. This study was conducted to develop a vaccine from local strains of P. multocida and evaluate its performance. Inactivated vaccine was prepared following the OIE standards in three adjuvant formulations (oil, alum and gel). The performance of the different formulations was evaluated at different dose rates (0.5 and 1 mL) and routes (subcutaneous, SC and interamuscular, IM) in vaccination-challenge experiment in a total of 160 (six weeks old) chicken. The vaccinated groups showed significantly higher (P<0.05) mean antibody titer at day 21 (1365.49±376.97) and day 35 (1707±193.95) post-vaccination compared to the mean value at day 0 (200.01±4.91) and that of the unvaccinated group (196.72±10.51.147). The highest antibody titer obtained was for group vaccinated with 0.5 mL of alum-adjuvanted vaccine given IM (2472.96±603.47). The differences in antibody titer among vaccinate...
Journal of Advanced Veterinary and Animal Research, 2017
Objective: The objective of this study was to evaluate some hematological parameters in commercial layers inoculated with two virulent Pasteurella multocida serotypes. Materials and Methods: A total of 84 twenty-week-old black Harco layers were randomly assigned to seven groups (A, B, C, D, E, F and G) with 12 birds per group. 1mLof live attenuated fowl cholera (FC) vaccine was administered subcutaneously at 24 weeks of age to groups A and B, emulsified inactivated (killed) FC vaccine was administered dosed at 0.5 mL per bird subcutaneously at 24 weeks of age to groups C and D, groups E and F were not vaccinated, while group G served as control. Groups A, C and E were inoculated with P. multocida serotype A:1 and groups B, D and F were inoculated with P. multocida serotype A:3. Using McFarland Standard, each bird received a dose of 0.5 mL (0.1 mL intranasally and 0.4 mL intramuscularly) containing 4.5 x 10 8 cfu/bird. Results: For PCV (P≤0.2692 and P≤0.7643) and HB (P≤0.2806 and P≤0.7266) on day 2 and 10 post inoculation, there was no significant difference between the vaccinated, non-vaccinated groups and control group G. However, there was a highly significant difference P≤0.05 in the mean concentrations of ALP between the control group G (67.67±1.453 u/l) vaccinated groups A (80.33±4.98 u/l), B (81.33±2.60 u/l), C (75±6.35 u/l), and D (84±5.132 u/l) and unvaccinated groups E (104±1.528 u/l), and F (78 ±3.512 u/l) post inoculation. Conclusion The PCV significantly decrease P≤0.05 in layers vaccinated and inoculated with P. multocida but increase in unvaccinated layers inoculated P. multocida. The mean serum ALP concentration significantly increase P≤0.05 in unvaccinated layers inoculated with P. multocida when compared to layers vaccinated and inoculated with P. multocida.