In Process Quality Control Factors Affecting Potency of Fowl Cholera Vaccine (original) (raw)
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Journal of World's Poultry Research
Fowl cholera is a septicemic respiratory complex caused by Pasteurella multocida, widely distributed in poultry and other avian species and of major economic importance. A total of 37 different inactivated Pasteurella multocida vaccines from different sources either locally prepared or imported from different sources were comparatively tested for relative potency following both single dose and booster dose vaccination assays. The study objective was to minimize the time factor exhausted in the evaluation processes of the inactivated fowl cholera vaccines. So it is planned to compare between single and booster dose vaccinations and their related potency. Correlation between protection associated with the single dose and booster dose vaccination were evaluated and average requirement for protection was 43.7% in single dose vaccination assay compared to 76.2 % associated with booster dose vaccination assay. In the same concern, the correlation between both assays for the seroconversion was estimated using ELISA and the minimum requirement was 1.8× cut off value in the single dose vaccination assay compared to 2.25× cut off value in the booster dose vaccination assay. In conclusion, single dose vaccination assay could be valuable in the evaluation of inactivated fowl cholera vaccines through determination of protection indices and/or estimation of humoral immune response if the above mentioned data is considered.
Development of washed cell fowl cholera vaccine in Bangladesh
2004
An experiment was conducted to develop washed cell fowl cholera (WCFC) vaccine with virulent avian Pasteurella multocida (PM 38) serotype 1 (X-73). A total of 20 Fayoumi birds of either sex of 10 weeks aged were divided into two groups as group A (immunized with washed cell fowl cholera vaccine) and group B (unvaccinated control). Primary vaccination was given through IM route in each birds of group A and booster dose was given through SC route after 15 days of primary vaccination. The presence of antibody against P. multocida was determined by slide agglutination test (SAT) and growth inhibition test (GIT). The degree of antibody levels of prevaccination and post vaccination sera were determined by passive haemagglutination assay (PHA). Sera mean PHA titres at 15, 21, 28 and 42 days post-vaccination in group A were 30.4±4.43, 46.4±6.06, 67.2±11.14 and 134.4±22.28 respectively. The present results revealed that WCFC vaccine worked satisfactory in terms of protection rate against Avian Pasteurellosis. It was also demonstrated that experimental WCFC vaccine conferred 80% protection against challenge infection when all chickens of control group failed to survive against challenge infection.
Journal of Bacteriology & Mycology: Open Access
A total of 37 different inactivated P. multocida vaccines from different sources either locally prepared or imported from different sources were comparatively tested for relative potency following both single dose and booster dose vaccination assays. The study objective was to minimize the time factor exhausted in the evaluation processes of the inactivated fowl cholera vaccines. So it is planned to compare between single and booster dose vaccinations and their related potency. Correlation between protection associated with the single dose and booster dose vaccination were evaluated and average requirement for protection was 43.7% in single dose vaccination assay compared to 76.2 % associated with booster dose vaccination assay. In the same concern, the correlation between both assays for the seroconversion was estimated using ELISA and the minimum requirement was 1.8X cut off value in the single dose vaccination assay compared to 2.25X cut off value in the booster dose vaccination assay. In conclusion, single dose vaccination assay could be valuable in the evaluation of inactivated fowl cholera vaccines through determination of protection indices and/or estimation of humoral immune response if the above mentioned data is considered.
Efficacy of Inactivated Fowl Cholera Vaccine in Chickens
2018
Fowl cholera is highly prevalent bacterial disease in poultry population of India. Several areas of India have been reported with outbreaks of fowl cholera. Fowl cholera severely affects the health of flocks, causes morbidity and mortality. Drop in egg production with worsened egg quality also results due to poor health of flock. The disease is also prevalent in village chicken and vaccination is the effective tool to control the prevalence of the disease. In present research, 30 specific pathogen free birds were divided into vaccinated (Group 1; 20 birds) and control (Group 2; 10 birds) groups. Birds from group 1 were vaccinated with inactivated fowl cholera vaccine and 14 days later given booster dose. After 21 days of booster dose, both groups were challenged with virulent strain of fowl cholera disease. At 7 days interval blood was collected and serology was performed for presence of antibodies against the disease with ELISA technique. The vaccine was found effective with respec...
Efficacy of Montanide Isa-70-vg as Adjuvant to Fowl Cholera Vaccine
Journal of Veterinary Advances, 2015
Water in oil emulsion fowl cholera vaccine was prepared using Montanide ISA-70-VG oil as alternative adjuvant to the used white mineral oil with span-80. The two vaccines were comparatively evaluated in SPF chickens. Each vaccine was inoculated in a group of 40 chickens while the third group of 40 chickens was kept as non-vaccinated control. It was noticed that the Montanide ISA-70-VG formulated vaccine induced mild transient local inflammation in comparison with those induced by the mineral oil formulated vaccine. Challenge test and application of indirect haemagglutination test on serum samples obtained from all chicken groups revealed that Montanide ISA-70-VG formulated vaccine induced earlier and higher immune response than that induced by the mineral oil formulated vaccine. So it could be recommended to use Montanide ISA-70-VG as adjuvant to fowl cholera vaccine aiming to provide early and high immune response able to protect chickens against fowl cholera infection.
Comparative efficacy of BAU-fowl cholera and DLS-fowl cholera vaccines in indigenous chicken
Research in Agriculture Livestock and Fisheries
The present study was conducted to determine the immune response induced in indigenous chicken produced against BAU-FC and DLS-FC vaccines with their efficacy study against Pasteurella multocida. A total of forty (40) chickens were selected and divided into Group A (15), Group B (15) and Group C (10). Group A and B were vaccinated with BAU-FCV and DLS-FCV, respectively at the dose rate of 0.5 ml through SC at six weeks of age followed by boostering at 10 weeks of age while Group C was kept as unvaccinated control. Sera samples were collected after primary and booster vaccination and antibody titre was determined by Passive hemagglutination (PHA) test. The mean PHA titres recorded at 4 weeks after primary vaccination was 51.20 ± 7.84 in birds of group A and 38.40 ± 6.40 in birds of Group B. After booster vaccination, mean PHA titer was found 140.80 ± 31.35 at 16 weeks of age in case of BAU-FC vaccinated group and 115.20 ± 12.80 in case of DLS-FC vaccinated group. The mean PHA titer w...
2019
Fowl cholera caused by Pasteurella multocida is among the serious infectious diseases of poultry in Ethiopia. This study was conducted to develop a vaccine from local strains of P. multocida and evaluate its performance. Inactivated vaccine was prepared following the OIE standards in three adjuvant formulations (oil, alum and gel). The performance of the different formulations was evaluated at different dose rates (0.5 and 1 mL) and routes (subcutaneous, SC and interamuscular, IM) in vaccination-challenge experiment in a total of 160 (six weeks old) chicken. The vaccinated groups showed significantly higher (P<0.05) mean antibody titer at day 21 (1365.49±376.97) and day 35 (1707±193.95) post-vaccination compared to the mean value at day 0 (200.01±4.91) and that of the unvaccinated group (196.72±10.51.147). The highest antibody titer obtained was for group vaccinated with 0.5 mL of alum-adjuvanted vaccine given IM (2472.96±603.47). The differences in antibody titer among vaccinate...
Journal of Advanced Veterinary and Animal Research, 2016
Objectives: The objectives of this study were to isolate and identify Pasteurella multocida from fowl cholera (FC) suspected chicken, and to prepare and efficacy determination of formalin killed fowl cholera vaccine using the isolated P. multocida strain. Materials and methods: A total of five suspected dead chickens were collected from Brothers Poultry Farm located at Gazipur district, Bangladesh. The samples were processed and the P. multocida was isolated through conventional bacteriological techniques, were finally confirmed by polymerase chain reaction using P. multocida specific primers targeting cap gene. The P. multocida isolate was used to develop a formalin killed fowl cholera vaccine. The efficacy of the newly prepared vaccine was determined in Starcross-579 chickens (n=30) aging 15 weeks either by injecting 1 mL (group-A; n=10) or 0.5 mL (group-B; n=10) vaccine containing approximately 3.2x10 8 CFU/mL P. multocida organism; 10 birds were kept as unvaccinated control. The sera from the vaccinated and control birds were collected and were subjected for antibody titre determination by enzyme-linked immunosorbent assay (ELISA). Finally the vaccinated birds were challenged using virulent strains of P. multocida to confer the protection against FC. Results: P. multocida could be isolated from both the samples. The formalin killed vaccine prepared from the isolated bacteria was subjected for the determination of antibody titre in chicken, and found that the antibody titres in the birds of group A and group B were 4.513 and 4.07 respectively after primary vaccination, and 4.893 and 4.37 respectively after booster vaccination. Most of the vaccinated birds were found to be survived after challenging with virulent strain of P. multocida. Conclusion: It is concluded that the causal agent of FC (P. multocida) was successfully isolated from FC affected dead chickens. The prepared formalin killed fowl cholera vaccine induces protective immune response and conferred protection against challenge infection caused by the virulent strain of P. multocida.
archives of razi institute, 2021
Cholera toxin (CT) is one of the most well-known immunostimulants. Mammalian studies have shown that CT can generate immune responses against antigen. However, it has not exhibited a definite effect on poultry yet. In this study, focusing on a cost-effective method, we investigated the effect of different concentrations of cholera toxin obtained from Vibrio cholerae biotype El Tor and serotype Inaba on the immunogenicity of infectious bronchitis vaccine. After culturing and concentrating CT, different concentrations of cholera toxin (0.1, 1, 2 and 5 micrograms) were combined with avian infectious bronchitis (IB) vaccine strain H120 produced by Razi Vaccine and Serum Research Institute (RVSRI) and, at 7 days of age, inoculated via eye drop administration in 24 specific-pathogen-free chickens (7 groups of 6 chicks, which 4 experimental groups and 2 groups for negative controls (PBS and toxin) and one positive control group). Blood samples were taken weekly from the wing veins of the c...
Microbes and Health, 2013
The research work was performed for the isolation and identification of Pasteurella multocida from field cases, preparation of oil adjuvanted vaccine from isolated strain and determination of its efficacy. Samples were collected from suspected dead birds of three poultry farms of Bangladesh (Code name: M and R). The P. multocida isolates were Gram negative, non-motile, non-spore forming rod occurring singly or pairs and occasionally as chains or filaments. Biochemically P. multocida ferment basic sugar and consistently produced acid except from maltose and lactose. After isolation formalin killed oil adjuvanted Fowl cholera vaccine was prepared in Laboratory of the Department of Microbiology and Hygiene, BAU and this experimental vaccine (3.2x10 8 CFU/ml) was administered in nine weeks old White Leg Horn chickens at the different dose rate through intramuscular (IM) route in each selected group A (1ml alum precipitated vaccine), B (0.5ml alum precipitated vaccine), C (1ml oil adjuvanted vaccine) and D (0.5ml oil adjuvanted vaccine). Pre-vaccinated sera were collected from all groups of birds. The mean of Passive Hemagglutination (PHA) titers of post-vaccination were 51±17.8, 76.8±17, 89.6±17, and 115±17.81 in group A, B, C and D respectively which consist of 5 birds in each. The vaccine produced better immune response when boostering with the similar dose and route at 15 days after primary vaccination. The mean PHA titers were higher at group D than other groups after boostering. Challenge infection was conducted on all the vaccinated and control group (n=5) of birds after 15 days of vaccination which protect 93.75% of birds and the PHA titers from different groups analyzed to determine the protective capacity of vaccinated chickens against challenge exposure. It was demonstrated that experimental oil adjuvanted fowl cholera vaccine with 0.5ml dose produce higher immune response against challenge infection and found to be safe.