Bacteriological Profile and their Antibiotic Susceptibility Pattern in Diabetic Foot Ulcers in a Tertiary Care Hospital, Puducherry, India (original) (raw)
Related papers
2020
Background Diabetic foot ulcer infection cause great morbidity and mortality among diabetic patients and is a major cause of lower extremity amputation worldwide. This study aimed to determine the profile of aerobic bacteria and their antibiotic sensitivity patterns in diabetic foot infections (DFI) among different Wagner's grades. Methods This study was conducted during December 2017-March 2018 in a Diabetic Center, Sudan. A total of 152 diabetic patients with different grades of foot ulcers were randomly enrolled in the study. The patients were grouped using Wagner's classification. Tissue biopsies and deep swabs were collected from the ulcers for aerobic cultures. The cultured isolates were identified using phenotypic and biochemical properties and their sensitivity to commonly used antibiotics, Colistin, Aikacin, Ciprofloxacin, Augmentin, Ceftazideme, Gentamicin, Clindamycin, Ceftriaxone Meropenum. Cotrimoxazole, Erythomycin, Oxacillin and Vancomycin. Fusidic acid, Imepenem, and Piperacillin was tested using the Kirby Bauer disk diffusion method. Results The mean age of the patients was 54.31 (SD ± 12.1) years, male to female ratio of 8: 1. The mean duration of diabetes was 14 (SD ± 8) years. The ulcers varied in duration from 1 day to 10 years. of 152 samples 181 aerobic bacteria were isolated. Cultures yielded 1-3 isolate per culture. The maximum number was isolated from grade 3 group followed by long standing ulcer LSU group 50.8% and 28% respectively. Polymicrobial infection was higher in LSU (30.4%). The isolates were mostly Gram-negative bacteria. The most frequent were proteus spp. (35.3%), S. aureus MRSA 14.4% and Coliform 12.2% respectively. The most common isolates in grade 3 were P. Mirablis, Staphylococcus and Coliform and in long standing ulcers were P. Mirablis, S. aureus MRSA and Coagulase negative staphylococcus respectively. Conclusion Gram-negative bacteria were more prevalent and the most frequent pathogens were Proteus spp. The
International Journal of Research in Medical Sciences, 2014
Diabetes mellitus is a worldwide disorder and affects many people. Type 2 diabetes is the most common form of diabetes in developing countries like India, Africa and China. At present 31.7 million people are diabetic in India. Hence, it has been labelled as "The diabetic capital of the world". 1 Diabetes is considered to be a serious disorder not just for its predominance but for its complications. One such important complication is the ABSTRACT Background: Diabetic foot infections are important cause of morbidity and mortality among persons with diabetes mellitus. The reported prevalence rates in India range from 0.9-8.3%. Diabetes foot lesions are the leading cause of non-traumatic amputations worldwide. A study has been conducted to isolate and find the antibiotic susceptibility pattern of the bacteria from diabetic foot infections from the patients of Kancheepuram district, Tamil Nadu, India. Methods: Sixty patients previously diagnosed or newly diagnosed as diabetic, presented with lower extremity infection attending Tagore medical college and hospital and its peripheral centres were selected for the study. Various specimens (pus, wound exudates, or tissues biopsy) for microbiological studies were obtained from the infected region. The specimens were cultured on blood agar and MacConkey agar for aerobic / facultative anaerobic organisms and on Neomycin Blood Agar for anaerobic organism. The plates were then incubated at 37°C. For anaerobic culture the plates were incubated in the McIntosh anaerobic jar. Isolates obtained are identified by standard laboratory techniques. Results: The result showed that Pseudomonas aeruginosa (48.3%) is the predominant bacterium followed by Staphylococcus aureus (38%) and other bacteria. The anaerobic bacteria are also isolated from the diabetic foot ulcers. The Peptostreptococcus species (26.7%) are the predominant bacteria followed by other bacteria. Further the results showed that 22 patients (37%) showed the multi-bacterial infection and remaining 38 patients (63%) showed mono bacterial infection. The drugs like amikacin, cefepine, ciprofloxacin, cotrimoxazole and roxythromycin are sensitive to many gram positive bacterial isolates. Conclusion: The present study has given the data of various bacteria encountered in the diabetic foot ulcer in the district of Kancheepuram, Tamil Nadu, India and its antibiotic sensitivity pattern. The results clearly reveal that there is no definite aetiology in diabetic foot infections. Many patients presented the infection with the involvement of many bacteria. Further it is evident that many bacteria are multi drug resistant and thus complicating the management of diabetic foot infections.
Microbiological Profile of Diabetic Foot Infections in a Tertiary Care Hospital in Navi Mumbai
Scholars Journal of Applied Medical Sciences, 2020
Original Research Article Diabetic foot ulcers are most common serious consequences of diabetes. The proper management of diabetic foot infection (DFI) requires a detailed knowledge about the microbial spectrum and their antibiogram. In this study, 123 cases with DFI were studied. Pus swabs and deep tissue/ bone samples were collected. We observed 74.8% & 16.2% cases were monomicrobial and polymicrobial in nature, respectively. Pseudomonas aeruginosa (29.5%) was the most commonly isolated organism followed by Staphylococcus aureus (16.6%) and Escherichia coli (12.8%). We observed 27.27% strains of Methicillin Resistant Staphylococcus aureus (MRSA) & 100% Extended Spectrum Beta Lactamases (ESBLs) strains of enterobacteriacae. All the gram positive organisms, Staphylococcus aureus, Enterococcus faecalis and Coagulase negative Staphylococcus were sensitive to vancomycin (100%) and Linezolid (100%). Pseudomonas aeruginosa isolates were 100% sensitive to Amikacin and Tobramicin, Piperacillin-Tazobactm and Ciprofloxacin. Klebsiella pneumoniae showed high resistance to Cefepime (87.5%) and Piperacillin-Tazobactm (75%). Escherichea coli isolate showed 100% sensitivity towards Gentamicin. Acinetobacter baumanni showed 100% sensitivity for Imipenem and Meropenem.
Bacterial Profile of Diabetic Foot Ulcer - Study From Western India
International Journal of Health Sciences and Research, 2016
Introduction: Diabetic foot ulceration and infections are a major medical, social, economic problem and a leading cause of morbidity and mortality, especially in the developing countries like India. Fifteen percent of all diabetics develop a foot ulcer at some point in their lives. Aim and objectives: To Study the bacterial profile of diabetic foot ulcer. To isolate and identify aerobic bacteria from diabetic foot ulcers and to determine their antibiotic resistance pattern Material and methods: This was a prospective study, conducted over a period of 1 year. 75 diabetic patients with foot ulcer attending the Surgery OPD were included in the study. Two swabs were taken from each patient into Amie’s transport medium and processed by standard techniques to isolate and identify the bacteria. Antibiotic susceptibility testing was done by Kirby-Bauer’s disc diffusion method for all the isolated bacteria. Results: A total of 110 aerobic organisms were isolated from 75 cases. Organisms isol...
International Journal of Current Microbiology and Applied Sciences, 2016
Diabetes mellitus is a metabolic disorder present with chronic hyperglycaemia and disturbances in carbohydrate, fat and protein metabolism. It is due to defects in insulin secretion, action or both. It manifested as damage or dysfunction in various organs like retina, kidney, Cardio vascular system, Cerebro vascular system, bone, joints & soft tissue like foot ulcer. It also includes physiological disturbances like sexual dysfunction. The major contributory factors for the high prevalence of the complications are; delayed diagnosis of diabetes, inadequate control of glycaemia,
International Journal of Basic & Clinical Pharmacology, 2014
Diabetes is a metabolic disorder of the endocrine system which plagues approximately 17 million people nationwide. Each year over 700,000 new cases are diagnosed; 12,000 to 14,000 of which are children, teenagers and young adults, while this life threatening disease can be controlled. Diabetes is often accompanied by serious complications, and still today there is no cure. 1 Foot ulceration and infection in diabetic patients is one of the major causes of morbidity, hospitalization and foot amputation. 2 This complication accounts for approximately 20% of hospital admissions in diabetic patients. 3 Diabetic foot infections include cellulitis, abscess, necrotizing fasciitis, septic arthritis, tendonitis and osteomyelitis. 4 Infections are often polymicrobial, Multi drug resistant and associated with inadequate glycemic control. There is a need for continuous surveillance of resistant bacteria to provide the basis for empirical therapy and reduce the risk of complications. Aims and objectives To isolate and identify the bacterial pathogens associated with Diabetic foot infections. To find out its antibiotic susceptibility pattern.
National Journal of Medical Research, 2012
Introduction: A Prospective study “Spectrum of Microbial flora in diabetic foot ulcer and its antibiotic sensitivity pattern” was carried out in a tertiary care hospital, Ahmedabad on 125 patients in which 85 were male patients and 40 were female patients. Material and Methods: Swabs samples were collected from the edge and margins of ulcers and organism were identified by gram staining culture and biochemical reactions. Results: Out of 125 specimens 108 specimens showed growth of organisms. Total 157 aerobic organisms were isolated from culture positive specimens. It represents an average of 1.25 organisms per case. Among these organisms, 130 gram negative and 27 gram positive organisms were isolated. Pseudomonas aeruginosa (30.57%) was predominant organism followed by Klebsiella spp. (22.29%). Staphylococcus aureus were 12.74% in which Methicillin resistant S. aureus (MRSA) was 55%. Conclusion: incidence of growth was 86.4% in which Pseudomonas aeruginosa (30.57%) is most common isolate. Organisms in mixed infections showed multidrug resistance as compared to single isolated strain. Diabetic foot infections are polymicrobial in nature. As the Wagner’s grade increased, the prevalence of isolates also increased.
International Surgery Journal, 2020
Background: Diabetic foot ulcer is one of the major surgical problem leading to hospital admission. Diabetic foot ulcer patients with uncontrolled diabetes may end up in forefoot amputation. Early aggressive debridement, control of blood sugar and empirical antibiotic therapy would reduce the morbidities in patients with diabetic foot ulcer. Further the knowledge of commonly isolated microbes and their antibiotic sensitivity pattern would be helpful to start empirical therapy. The purpose of this study was to determine the microbiological profile of diabetic foot infections (DFIs) and assess the antibiotic susceptibility of the causative agents.Methods: This cross-sectional study was conducted in 115 patients admitted with diabetic foot ulcer over a period of 9 months from October 2015 to June 2016 at the department of general surgery, Sri Manakula Vinayagar Medical College and Hospital, Pondicherry. Tissue scrapping samples were collected and processed as per standard guidelines.Res...
innovative publication, 2017
Introduction: One of the most important complications of diabetes mellitus is foot infection. Bacterial spectrum of diabetic foot infections vary greatly. The increasing association of multi-drug resistant (MDR) bacteria in diabetic foot infections further complicates therapy. Hence, we aimed at determining the prevalence of bacteria in diabetic foot ulcers and their anti-biogram. Materials and Methods: This is a prospective, observational study. A total of 217 infected diabetic wound samples (pus swab/discharge) were collected from patients seen both IP department and OP departments of Vinayaka Mission's Medical College & Hospital. Antimicrobial resistance pattern was performed, as per standard microbiological procedures including methicillin resistant S. aureus and extended spectrum of beta lactamase (ESBL). Results: Overall 207 bacteria were isolated. Among them, 122 (58.94%) were Gram negative bacilli and others were Gram positive cocci, 85(41.06). The most common isolate was Pseudomonas (23.67%) followed by Staphylococcus aureus (22.70%), Coagulase negative Staphylococci (15.94%), Klebsiella species (14.97%), Escherichia coli (9.18%). Among 122 Gram negative bacilli, 57 were identified as ESBL producing strains. A total of 47 isolates of Staphylococcus aureus were recorded, 22 were identified as MRSA strains. Majority of Gram negative isolates were susceptible to piperaciillin/tazobactum followed by amikacin. All isolates remained susceptible to cefeperazone/sulbactum and imipenam except non fermenting Gram negative bacilli. Conclusion: Regular monitoring of bacterial susceptibility patterns helps in guiding clinician to choose apt antibiotic to treat infected diabetic foot. Treatment should be initiated only after performing culture and sensitivity testing. Therefore, the rapid propagation of multi drug resistance can be prevented.