Potential role of probiotic bacteria as antioxidants agent (original) (raw)
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ANTIOXIDANT ACTIVITY AND BIOLOGICAL EVALUATIONS OF PROBIOTIC BACTERIA STRAINS
The antioxidant activity of four probiotics strains belongs to the genera Lactobacillus (two strains), Bifidobacterium as well as Probionebacterium were investigated through the DPPH • and ABTS •+ scavenging ability of the cell free extract of bacteria of the probiotic strains comparing with the standard antioxidant ascorbic acid and BHT. The results of the DPPH scavenging potential of cell free extract showed that the maximum antioxidant activity with Probionebacterium freudenreichii ( 97.75 % ) and it was significant increased compared with vit.c and BHT followed by Lactobacillus retueria (96.74 % ) activity. All cell free extracts showed highly scavenging potential against ABTS radical. The present study evaluated the survival of free probiotic bacteria under acidic conditions (pH 2 and pH3) during 3h, L.GG showed intolerance to pH2, but showed more acid tolerance at pH3. In contrast, the other strains showed more acid tolerance at pH values where incubation of strains for 3h at pH2 resulted in a decrease of about one log cycle, while at pH3 the decrease ranged from 0.2 to 0.7 log cycles. A similar decrease in cell viability was observed in the case of 1% and 2% bile salt for L.reuteri and B.breve, the decrease ranged from 0.4 to 0.6 log cycles, while in case of L.GG and P. Jensenii the decrease in cell viability was about 1.5 log cycle. However, free cells were survived at 2 % bile salt. The biological evaluations of biscuit incorporated with four encapsulated probiotic bacteria strains and biscuit without encapsulated probiotic bacteria were studied in albino rats. The results showed that non significant change in (, Total cholesterol and HDL-C) but there were significant change in ( serum triglycerides and LDL-C ). The safety of experimental diet was studied through serum liver and kidney functions where non significant change in liver function (serum alanine aminotransferase, aspartate aminotransferas and alkaline phosphatase). Also non significant change in kidney function (Creatinine & Urea).
Antioxidant properties of potentially probiotic bacteria: in vitro and in vivo activities
Applied Microbiology and Biotechnology, 2013
Thirty-four strains of lactic acid bacteria (7 Bifidobacterium, 11 Lactobacillus, 6 Lactococcus, and 10 Streptococcus thermophilus) were assayed in vitro for antioxidant activity against ascorbic and linolenic acid oxidation (TAA AA and TAA LA ), trolox equivalents (TEAC), intracellular glutathione (TGSH), and superoxide dismutase (SOD). Wide dispersion of each of TAA AA , TAA LA , TEAC, TGSH, and SOD occurred within bacterial groups, indicating that antioxidative properties are strain specific. The strains Bifidobacterium animalis subsp. lactis DSMZ 23032, Lactobacillus acidophilus DSMZ 23033, and Lactobacillus brevis DSMZ 23034 exhibited among the highest TAA AA , TAA LA , TEAC, TGSH values within the lactobacilli and bifidobacteria. These strains were used to prepare a potentially antioxidative probiotic formulation, which was administered to rats at the dose of 10 7 , 10 8 , and 10 9 cfu/d for 18 d. The probiotic strains colonized the colon of the rats during the trial and promoted intestinal saccharolytic metabolism. The analysis of plasma antioxidant activity, reactive oxygen molecules level, and glutathione concentration, revealed that, when administered at doses of at least 10 8 cfu/d, the antioxidant mixture effectively reduced doxorubicin-induced oxidative stress. Probiotic strains which are capable to limit excessive amounts of reactive radicals in vivo may contribute to prevent and control several diseases associated with oxidative stress.
In vitro study of antioxidant and antibacterial activities of Lactobacillus probiotic spp
Folia Microbiologica, 2017
This study investigated the influence of aeration and minimal medium conditions on antioxidant and antibacterial activities of 21 probiotic Lactobacillus strains isolated from dairy products. The probiotic potential of the isolates was evaluated by pH and bile tolerance. Random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) was used to confirm the phenotypic identification of isolates. Antioxidant producer isolates were screened by resistance to reactive oxygen species (ROS). The antioxidant and antibacterial activities of extracellular materials after 48 h fermentation with antioxidative strains were determined using 2,2diphenyl-1-picrylhydrazyl (DPPH) and broth microdilution assays, respectively. The results indicate that the antioxidant capacity of supernatants was increased by using of both minimal medium and agitation. The antibacterial activity was increased in minimal medium, but there has nearly no change in the antibacterial properties by using both agitation and minimal medium. The maximum antibacterial activity was observed during mid-exponential phase until the beginning of the early-stationary phase, but the maximum antioxidant activity was detected at the stationary growth phase. There is a significant relationship between antioxidant and antibacterial activities of the cell-free probiotic extracts, and their production rates are closely related to the fermentation type. The bioactive materials from probiotics could be extracted in a large amount at an appropriate time under a suitable condition.
Lactic Acid Bacteria (LAB) with probiotic potentials isolated from fermented food of Indian origin were investigated for antioxidant activities. The antioxidant potential of the Cell Free Extracts (CFEs) of the nine probiotic isolates (GS3, GS4, GS7, GS9, GS14, GS16, GS17, GS20 and GS21) were investigated through different assays namely the Reducing power, DPPH free radical scavenging and β-Carotene bleaching assay. The Cell Free Extracts of isolates demonstrated reducing activity in the range of 60 to 92.66 µg/µl ascorbic acid equivalence; with the maximum activity being observed with the CFE of Pediococcus GS4 isolate (92.66µg/µl). DPPH free radical scavenging activity of the CFE of isolates was observed in the range of 48.67-91.26%. The β-Carotene bleaching assay was performed on CFE of isolates showing maximum activity in the above two assays. 150 µl of CFE of GS4 exhibited the maximum zone of colour retention of 22.00±1.000 mm and Butylated hydroxy toluene (1mg/100 μl) as positive control exhibited an average zone of colour retention of 27.00±0.577mm. 150 µl CFE of GS17 exhibited lower antioxidant activity in comparison to CFE of GS4 with the average zone of colour retention being 20.30±0.577mm. This extends the functional use of Probiotics as nutraceutical with antioxidant potential and may find therapeutic application for degenerative diseases related to ageing.
Antioxidative potential of lactobacilli isolated from the gut of Indian people
Molecular Biology Reports, 2012
Oxidative stress is one of the major causes of degenerative conditions occurring at cellular level with serious health implications. This study was aimed at investigating the antioxidative potentials of probiotic lactobacilli of Indian gut origin and their ability to augment antioxidant defense enzyme systems in the host cells under oxidative stress conditions. A total of 39 Lactobacillus cultures were assessed for their resistance against reactive oxygen species. Most of the cultures were moderately to strongly resistant towards 0.4 mM H 2 O 2. The Lactobacillus isolate CH4 was the most H 2 O 2 resistant culture with only 0.06 log cycle reduction. Majority of the cultures demonstrated high resistance towards hydroxyl ions and Lp21 was the most resistant with log count reduction of 0.20 fold only. Almost all the cultures were also quite resistant to superoxide anions. Lp21 also showed the highest superoxide dismutase content (0.8971 U). Amongst the 39 cultures, Lactobacillus spp. S3 showed the highest total antioxidative activity of 77.85 ± 0.13 % followed by Lp55 (56.1 ± 1.2 %) in terms of per cent inhibition of linolenic acid oxidation. Lp9 upregulated the expression of superoxide dismutase 2 gene in HT-29 cells both at 0.1 mM (1.997 folds) and 1.0 mM H 2 O 2 (2.058 folds) concentrations. In case of glutathione peroxidase-1, Lp9, Lp91 and Lp55 showed significant (P \ 0.001) up-regulation in the gene expression to the level of 5.451, 8.706 and 10.083 folds, respectively when HT-29 was challenged with 0.1 mM H 2 O 2. The expression of catalase gene was also significantly up-regulated by all the cultures at 0.1 mM H 2 O 2 conditions. It can be concluded that the antioxidative efficacy of the putative probiotic lactobacilli varied considerably between species and strains and the potential strains can be explored as prospective antioxidants to manage oxidative stress induced diseases.
Anais da Academia Brasileira de Ciências
In recent years, it is very important to fi nd more safe and natural antioxidants than biological sources to replace synthetic antioxidants. The antioxidant properties of 22 lactic acid bacteria were investigated in vitro in the present study. The conducted in vitro antioxidant assays included scavenging the 2,2-diphenyl-1-picrylhydrazil (DPPH) free radical, metal (Fe +2) ion chelation, hydroxyl radical and superoxide radical scavenging properties, and anti-lipid peroxidation. Analysis of DPPH free radical scavenging property of microorganisms demonstrated that Lactobacillus plantarum IH14L (90.34 ± 0.40%) strain exhibited the highest activity. The highest Fe +2 chelation activity was observed with Lactobacillus curvatus GH5L (75.98 ± 5.35%), while the lowest chelation activity was observed with L. plantarum IH14L (19.80 ± 0,05). The highest and lowest hydroxyl radical scavenging strains were L. plantarum IH16L (82.25 ± 1.60%) and L. plantarum IH26L (35.60 ± 4.50) strains, respectively. Comparison of superoxide radical scavenging activities of the microorganisms demonstrated that these activities ranged between 21.63 ± 1.32% and 7.22 ± 0.04%. A positive correlation was identifi ed between the hydroxyl radical scavenging activity of the microorganisms and the anti-lipid peroxidation property. It was found that they had the potential for use in nutritional and probiotic applications as protective and natural antioxidants.
Study of Probiotic and Antioxidant activity of Lactobacillus spp
Research Journal of Pharmaceutical, Biological and Chemical Sciences
Curd is a source of probiotic Lactobacilli. In the present study, ten homemade curd samples were collected from different regions of Vellore district, Tamil Nadu, India. Among the samples, four different strains of Lactobacillus were isolated and identified based on their colony morphology and biochemical characteristics. It was observed that isolated Lactobacillus spp. were resistant to inhibitory substances like NaCl (1-9%), bile-salt (0.05-0.3%), and showed good growth in the acidic condition, while maximum growth was observed at pH around 6. The isolates were examined for their antibacterial activity against four different test pathogens, and found that growth of all pathogens are inhibited to some extent but maximum zone of inhibition was observed against E.coli (33mm) and no zone of inhibition against Pseudomonas aeruginosa after 24 hour incubation. The isolated Lactobacillus spp. showed good survival abilities in acidic (pH 4) and alkaline (pH 8) conditions. Isolated Lactobacilli were able to produce organic acid in skim milk which was determined by titrimetric method. In the antibiotic Sensitivity test, LB06, LBS3, LB02 and LBS1 were found to be sensitive against Ciprofloxacin and Erythromycin. LB06 was resistant against Ampicillin and Bacitracin while LB02 was resistant against Ampicillin. In the DPPH scavenging assay, LB06 and LBS3 showed highest inhibition of 56.84% and 55.86% in 1000 μl/ml compared to the positive control (BHT) 69.29%. The IC50 value was found to be 750μl/ml.
Probiotic Properties and Antioxidant Efficiency of Lactobacillus Plantarum 15 Isolated from Milk
Journal of microbiology, biotechnology and food sciences, 2019
Many researchers are focusing on the investigation of new molecules and substances to help the body fighting oxidative stress; among them are probiotic bacteria. In this study we investigated the probiotic properties and the antioxidative activity of Lactobacillus plantarum 15 isolated from milk. Therefore, to evaluate probiotic potential of the selected strain, the antibacterial activity and resistance to acidic pH and bile salts conditions were evaluated. Moreover, several methods were used to analyze the antioxidant potential of this strain. The evaluation of probiotic properties showed that Lb. plantarum 15 resists to acidity and to bile salts (survival rate was 86.40% and 80.58%, respectively). The isolate showed a broad inhibitory activity against several bacterial strains. The antioxidant potential assessed by the DPPH method showed that Lb. plantarum 15 cells as well as the cell-free supernatant have important scavenger effect (82.65% and 72.21%, respectively). Furthermore, ...
Cytotoxicity assay and antioxidant activities of the lactic acid bacterial strains
African Journal of Microbiology Research, 2012
The present study was designed to evaluate the safety degree of lactic acid bacterial (LAB) usage on mammalian cells and anti-oxidative ability of their intact cells, and both extra-and intra-cellular extracts. The non-toxic doses of eleven different LAB strains were detected by cytotoxicity assay test on peripheral blood mononuclear cells (PBMC) using neutral red stain. The antioxidant properties of LAB strains were evaluated biochemically using OH scavenging assay, superoxide scavenging assay, hydrogen peroxide scavenging assay, DPPH radical scavenging assay, reducing ability assay, inhibition of lipid peroxidation assay, and inhibition of human blood erythrocyte haemolysis assay. In addition, in-vitro antioxidant evaluation was carried out by using Flow Cytometric assay, on which the ROS produced by the stress factor Helicobacter pylori LPS on PBMC. The cytotoxicity results clarified that the safe doses of LAB extracellular extracts reached up to 20% while, the intracellular extracts safe doses reached up to 14%. Out of eleven tested LAB strains, Lactobacillus plantarum, Lactobacillus bulgaricus DSMZ 20080 and Lactobacillus acidophilus showed maximum free radical scavenging abilities at two levels: at intact cell level and at extract levels. In addition, in-vitro assay showed that ROS scavenging ability reached up to 100% in case of using mixture of both extracts of these strains.