Radical Scavenging Activity and Quercetin Content of Muntingia calabura L. Leaves Extracted by Various Ethanol Concentration (original) (raw)
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Kontribusia (Research Dissemination for Community Development)
Astract Muntingia calabura plant is a plant in Indonesia that has a variety of functions. It is used as a herbal ingredient to treat certain diseases and also as an antibacterial as well as natural antioxidants. Because inside the leaves there are various bioactive compound that can be used for the sake of herbal making. The purpose of this study is to do early screening of Muntingia calabura leaf extract using ethanol and aquos solvent. The method used in extraction is maceration exaction. The phytochemical analysis performed are total phenols, total flavonoids and antioxidants (IC50). The results showed a total phenol in the ethanol extract was 361.22 mg of GAE/g total flavonoids was 42.46 mg QE/g and antioxidant activity (IC50) was 131.22 μg/mL. At the aquos extract, total phenol was 267.61 mg of GAE/g, total flavonoids was 16.22 mg QE/g and antioxidant activity (IC50) was 129.31 μg/mL. By seeing the total phenol and antioxidants in both the extract (ethanol and aquos), the Munti...
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Malus domestica (Apple) is one of the most widely cultivated cash crops of Nepal. Jumla and Mustang are two major pocket areas for the production of apple. Flavonoids including quercetin and rutin are potent antioxidants present in apples. This study was designed to quantify and compare the presence of quercetin and rutin in different plant parts (peel, leaf, and bark) among various cultivars of Malus domestica from two pocket zones of Nepal. A new HPLC-UV method was developed and validated for the quantification of quercetin and rutin. Polyphenols, flavonoids, and carbohydrate contents were determined by colorimetric methods. 2,2′-Diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay was carried out to measure in vitro antioxidative activity. Acid hydrolysis of each extract was carried out by the standard method to measure aglycone quercetin content after hydrolysis of its glycosides. The total rutin content ranged from 3.69 ± 1.34 to 374.50 ± 2.35 mg/100g dry extract weig...
Open Access Macedonian Journal of Medical Sciences, 2022
BACKGROUND: Seri (Muntingia calabura L.) leaves are a plant that is often found and have not been used in various treatments even though it is reported to have various groups of bioactive compounds such as phenolic, flavonoids, tannins, saponins, steroids, and triterpenoids. AIM: This study aimed to determine the total phenolic content, antioxidant activity and identify the content of potential bioactive compounds contained in the ethyl acetate fraction from M. calabura leaves. METHODS: M. calabura L. leaves fraction was carried out by maceration method using ethanol followed by partition starting with n-hexane, chloroform, and finally ethyl acetate as solvent. The ethyl acetate fraction was continued for phytochemical screening for the content of bioactive compounds using standard reagents, determination of total phenol content by colorimetric method, determination of antioxidant activity using the DPPH method, and analysis of bioactive compounds using gas chromatography–mass spect...
Phytochemical Screening of Muntingia Calabura Fruit for Antioxidant and Cytotoxic Activities
Journal of Advanced Research in Applied Sciences and Engineering Technology
Muntingia calabura (M. calabura) is an indigenous fruit species that is widely distributed in Malaysia and traditionally believed for constituting medicinal properties, yet little-known and underutilized. Hence, this study aimed to perform an extraction of M. calabura fruit to identify phytochemicals potential by carrying out several analysis such as Total Phenolic Content, Fourier Transform Infrared Spectrum (FTIR), High Performance Liquid Chromatography (HPLC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. Besides, the antioxidant and cytotoxicity properties of M. calabura were also identified from Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC) and brine shrimp lethality tests. The finding suggested that further study on the modification of M. calabura extraction should be carried out to explore maximal potential of M. calabura as natural medicine and commercial use .
Quantitative and Qualitative Phytochemicals Analysis of Muntingia calabura
Journal of Biology Agriculture and Healthcare, 2014
Muntingia calabura is widely cultivated and has become one of the common roadside trees in Indonesia. The present study investigates the qualitative and quantitative analysis of the major bioactive constituents of plant Muntingia calabura in some solvents (water, methanol, ethanol, chloroform, ether, and citrid acid). Phytochemical screening method was used to identify qualitative analysis of bioactive component. While the quantitative analysis was analysed by UHLPC. The results showed the highest percentage extract concentration was resulted by methanol followed by water, ethanol, chloroform, ether and citric acid 13.15%, 11.93%, 9.57%, 9.04%, 4.52% and 3.69% respectively. The bioactive component ie., saponins, flavonoids and tannin were found in all those solvent. The higher concentration of flavonoid, saponin and tannin were resulted from polar solvent ie., water, methanol and ethanol. While non polar solvent has lower in concentration. Epigallocatechin Gallate (EGCG) and Genistein content of extract Muntingia calabura from methanol solvent were 135.15 µg/g and 136.29 µg/g respectively.
world journal of pharmaceutical sciences, 2017
Mussaenda glabrata (Hook.f.) Hutch. ex Gamble (Rubiaceae) is traditionally used as diuretic, antiasthmatic, antimicrobial and anti-inflammatory. In this prospective study to evaluate the chromatogram detection of chloroform and ethanol extract of Mussaenda glabrata leaf with standard flavonoid markers such as Rutin, Quercetin and Gallic acid by HPTLC techniques. HPTLC Chromatogram was developed in both the extracts by using Toluene-Ethyl acetate-Formic acid-Methanol (3: 6: 1.6: 0.4) as mobile phase. The identity of the bands of compounds 3-7 in both the extracts of Mussaenda glabrata were estimated by their UV absorption spectra with the standard marker rutin, quercetin, and gallic acid at 254 nm. By comparing with the Rf value of standard antioxidants markers. Quercetin was present in both extracts but ethanol extract contains nearly (0.56 %) when comparing with chloroform extracts, ethanol extract contain comparatively more rutin (0.09%) and gallic acid (0.32). Thus the present study provided a scientific validation for the traditional claims of Mussaenda glabrata revealed the presence flavonoids quercetin, rutin and gallic acid in extracts of Mussaenda glabrata.
Food Chemistry, 2008
Barks extracts of four different trees (Azadirachta indica, Terminalia arjuna, Acacia nilotica, and Eugenia jambolana Lam.) in three different solvents 80% methanol, 80% ethanol, and 80% acetone (solvent:water, 80:20 v/v) were evaluated for their antioxidant activity, total phenolic (TP), and total flavonoids (TF) contents. Antioxidant activity (AA) was determined by measuring reducing power, inhibition of peroxidation using linoleic acid system and 2,2 0 -diphenyl-1-picrylhydrazyl radical (DPPH) scavenging activity. Significant (P < 0.05) differences were observed in the TP, TF, inhibition of linoleic acid oxidation and DPPH Á scavenging activity of different bark extracts. Nevertheless, minute variation was observed in reducing power. All the bark extracts exhibited wide range of total phenolic, 7.8-16.5 gallic acid equivalents and total flavonoid contents, 1.59-4.93 catechin equivalents. Reducing power at 10 mg/mL extract concentration ranged from 1.34 to 1.87. Different bark extracts inhibited oxidation of linoleic acid by 44-90% while DPPH radical scavenging activity ranged from 49% to 87%. Extraction efficacy of components with antioxidative properties was lowering in the following order: ethanol > methanol > acetone. Good correlation was observed between TP and DPPH scavenging activity among the extracts. A. nilotica bark had the highest amounts of TP, ranging from 9.2 to 16.5 g/100 g, while the highest AA as measurement by inhibition of linoleic acid oxidation is offered by bark from E. jambolana Lam. The same tree showed the highest DPPH scavenging activity and reducing power. The correlation among the results of different antioxidant assays although revealed a strong relationship between some of the assays, however, a number of different methods may be necessary to adequately assess the in vitro antioxidant activity of a specific plant material.
In the present study, we evaluated antioxidant activity and total flavonoid content of selected medicinally important plant species of India. Extracts of fruits of Rauwolfia tetraphylla, seeds of Nyctanthus arbortristis and leaves of Calotropis gigantea were prepared for the study. In-vitro models like DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging activity was carried out to investigate the antioxidant activity of methanolic plant extracts. Total flavonoid content of the plants was estimated using quercetin as standard to find out their contribution
Journal of food and nutrition research
K. erecta S.; plant family Cyperaceae is locally used as food and medicinal flavor by the Hausas of the Northern Nigeria. This work aims to investigate the phytochemicals present in the whole plant extract, hence, determine the antioxidant activity, total flavonoid and total phenolic contents. The phytochemical screening was conducted on the crude extract using standard methods. The non-polar, moderately polar and highly polar (group 'N, M and P') fractions as well as a crystalline isolate (C) were subjected to antioxidant activity using DPPH and FRAP methods. TFC and TPC were determined in N, M and P only. Phytochemical screening result showed the presence of vital secondary phyto-constituents. The test fractions were able to scavenge DPPH radicals in a concentration dependant faction, the results were recorded as percentage inhibition and they all showed significant radical scavenging capacity (IC 50 : 0.311, 0.035, 0.297 and 1.089 mg/ml, respectively for N, M, P and C). N, M, P and C were able to reduce Ferric Chloride in a concentration dependent manner as well and the results were recorded as Ascorbic Acid Equivalent. The TFC and TPC were presented as Quercetin Equivalent and Gallic Acid Equivalent, respectively. The TFC were 37.50 and 43.75 mg, respectively of Quercetin in one gram of sample for N and M at 0.91 mg/ml while P had 59.03 mg/g, QE at 0.55 mg/ml. 50.000 and 46.430 mg/g, GAE, respectively were the total phenolic content of N and M at 0.09 mg/ml, respectively while 80.355 mg/g, GAE was recorded for P.
IDJP (Indonesian Journal of Pharmaceutics), 2019
Antioxidants are substances that can provide endogenous protection and exogenous oxidative stress by capturing free radicals. Many plants are efficacious as antioxidants, namely plants that contain polyphenols, especially flavonoids, so many are formulated as natural antioxidants. Plants such as Muntingia calabura, Syzygium cumini, Ocimum basilicum and Eleutherine bulbosa contain polyphenol compounds, especially flavonoids which are efficacious as natural antioxidants.This research aimed to study antioxidant activity derived from some potential plants using the DPPH method by calculating the IC50 value of each plant extract. This research method starts from the determination process to prove the validity of the plants used, the extraction process using the maceration method with 70% ethanol solvent, then the antioxidant activity of extracts from each plant was carried out using the DPPH method. This research starts from the determination process to ensure the correctness of the plants used, then the extraction process is carried out using the maceration method with 70% ethanol solvent. After that the antioxidant activity was determined from the four plants using the DPPH method to see the strongest IC50 value among the four plants. IC50 is the concentration of the sample to inhibit 50% of free radicals. The results of IC50 values fromethanol extract of Muntingia calabura leaves, Syzygium cumini leaves, Ocimum basilicum leaves and Eleutherine bulbosa bulbs, were 18.72; 63,84; 141.59 and 173.15 ppm. Ethanol extract of Muntingia Calabura has a smaller IC50 value of 18.72 ppm which has a very strong and most powerful antioxidant from the ethanol extract of Syzygium cumini, Ocimum basilicum and Eleutherine bulbosa.