Energy processes in isolated hepatocytes at different duration of insulin action (original) (raw)

Study of Lipogenesis and Inflammation Processes in Non-Alcoholic Fatty Liver Disease in the Model of Steatosis with the Use of HEPG2 Cells

Bulletin of the Saint Petersburg State Institute of Technology (Technical University), 2019

Non-alcoholic fatty liver disease (NAFLD) includes a spectrum of pathological changes in the liver, represented by steatosis (excessive accumulation of triglycerides-TG), steatohepatitis (the development of inflammation), fibrosis and cirrhosis. The article describes the making of a model of liver steatosis using HepG2 cells. The purpose of this work is to evaluate the TG and lipid accumulation and the proinflammatory cytokines production in experiments with this model. In order to make the model of steatosis, HepG2 cells were cultured in the presence of palmitic and oleic acids; the TG content was determined with the Randox Triglycerides kit, the lipid content-with flow cytometry using Nile Red dye, the cytokine content-with ELISA. Reliable increase of the TG, lipid and IL-8 levels was observed in the model of steatosis, which is typical for this disease, compared to control. Thus, the model of steatosis obtained with HepG2 cells is an adequate in vitro model of NAFLD.

Adaptive Capacity and Features of Energy Metabolism in Patients with Diabetes Mellitus Type 2

Mìžnarodnij Endokrinologìčnij Žurnal, 2013

The study of the processes of adaptation and characteristics of energy metabolism in patients with diabetes mellitus (DM) type 2 can serve as a basis for determining their role in the pathogenesis of this disease and contribute to the development of new methods of treatment and prevention. The aim of the study was to determine the level of adaptive capacity and state of the energy metabolism in patients with type 2 diabetes. Materials and Methods. The study involved 27 patients with DM type 2 (mean age — 56.9 years) and disease duration from 1 year to 20 years. Group of 15 apparently healthy volunteers, similar to the main group by age and sex, served as control one. Results. It is found that patients with DM type 2 had a reduction in adaptive abilities, which is based on violation of the energy metabolism of the body. In DM decompensation, the reduction of organism adaptation was more significant. Conclusions. Patients with DM type 2 were characterized by reduced level of adenosine...

State of Cell Energy Supply and Its and Correction in Intensive Care for Gastrointestinal Insufficiency Syndrome in Neonates

Актуальні проблеми сучасної медицини: Вісник Української медичної стоматологічної академії, 2018

In order to improve the intensive care for newborns with gastrointestinal insufficiency syndrome by providing the grounds to use amber acid, we carried out a randomized clinical trial involving 110 newborns with gastrointestinal insufficiency syndrome. The main group included newborns who received infusion with sodium succinate (n = 66) as a part of integrated therapy; the control group included the newborns who were not administered the above mentioned medication (n = 44). At the beginning of the study we observed a tendency towards the formation of lactate acidosis in the examined newborns, while no significant correlation was established between the severity of the gastrointestinal insufficiency syndrome and the indicators of cell energy supply. At the end of the study, the cell energy supply indicators varied in both groups by reducing the level of lactic acid, its ratio regarding pyruvic acid and increase in the activity of succinate dehydrogenase in newborns, who received ambe...

Biochemical parameters of liver functional state in rats upon long-term hypoacidity

Experimental and Clinical Physiology and Biochemistry, 2011

Significant deviation of biochemical parameters of liver functional state, such as albumin, urea, uric acid and total bilirubin concentration from control values upon prolonged hypoacidity induced by the 28-day-long administration of gastric parietal cells H+/K+-ATPase blocker omeprazole has been established. The activity of hepatic marker enzymes, such as alkaline phosphatase, cholinesterase, γ-glutamyltranspeptidase, alanine aminotransferase, aspartate aminotransferase and lactate dehydrogenase in blood samples of rats has also changed under current experimental conditions.

The role of leucine in hepatic ketogenesis

Surgery, 1988

Isolated hepatocyte studies demonstrated that leucine can be a precursor of ketone bodies. In this study we examine the relative contribution of leucine to hepatic ketogenesis in vivo. Three groups of conscious dogs with long-term indwelling catheters in the femoral artery, hepatic vein, and portal vein were studied. Group I (n = 3) animals were fasted overnight for 24 hours, and those in groups II and III (n = 4, each) were fasted for 62 to 68 hours (designated 3-day fast). Groups I and III received intravenous saline solution (0.9%) and served as controls. In group II selective acute insulin deficiency (SAID) was induced by a peripheral intravenous somatostatin (SRIF) infusion and intraportal glucagon (0.55 ng/body weight/min). Net hepatic production (NHP) of ketone bodies (kb) and leucine (leu) was measured by the arteriovenous difference technique. Hepatic conversion of leucine to ketone bodies was measured by continuous infusion of L-U-[14C]-leucine and by determination of the ...

Hepatic Microhaemocirculation Dynamics in Experimental Extrahepatic Cholestasis

Russian Journal of Gastroenterology, Hepatology, Coloproctology

Aim. Investigation of hepatic microhaemocirculation effects in experimental extrahepatic cholestasis.Materials and Methods. Experiments were carried out with a mixed group of 48 white mongrel male rats. Extrahepatic cholestasis was modelled in rats with via the ligation of common bile duct.Results. Animals with ligated common bile duct showed abnormalities in peripheral hepatic circulation manifested by a complex of intravascular, vascular and paravascular changes, hepatic angioarchitectonics disturbance, a marked growth of parenchymal dystrophic lesions in the form of post-haemorrhagic foci. The disturbance was pronounced towards a virtual degradation of hepatic parenchyma and the growth of “numb” zones with ceased circulation.Conclusion. The trials revealed microcirculatory changes in the liver associated with rheological disturbance of blood and, subsequently, the toxic impact of failed metabolism.

Liver Volume Restoration and Hepatic Microarchitecture in Small-for-Size Syndrome

Annals of transplantation : quarterly of the Polish Transplantation Society, 2015

BACKGROUND We investigated preoperative parameters that could work as markers of liver regeneration (LR), and tried to create an algorithm for therapeutic decision-making, looking at the clinical setting of post-hepatectomy liver failure (PHLF) after major liver resection for malignancies (LRM) and of the small-for-size syndrome (SFSS) after adult-to-adult living related liver transplantation (LRLT), considering PHLF and SFSS a single clinical entity. MATERIAL AND METHODS The clinical data of 2 series of 10 consecutive patients who experienced liver-specific complications after LRLT or LRM between 2008 and 2013 were analyzed. LR was evaluated by multidetector computed tomography (MDCT) and hepatic parenchymal findings with specific re-examinations of liver biopsies. The analysis was done according to demographics, tumor characteristics, and postoperative complications occurring within 90 days of surgery and codified within the Clavien classification. RESULTS A total of 13 cases of S...