The Effect of Calcium Hydroxide-Propolis on the Number of Macrofag Cells on the Wistar Rat Dental with Pulpa Performance (original) (raw)
Related papers
2021
Background: Cellular response of dental pulp to existing lesions, caused by infiltration of inflammatory cells that migrate from blood vessels to the site of injury. Macrophages are the cells that most appear when inflammation occurs. Treatment for maintaining pulp tissue vitality is called pulp capping. Calcium hydroxide as a pulp capping material has the ability to trigger the growth of dentin bridges or remineralization, but calcium hydroxide can induce migration and proliferation of inflammatory cells. Currently the field of dentistry is developing propolis as an alternative pulp capping material. That is because propolis has anti-inflammatory properties. Purpose: To analyze the effect of the combination of calcium hydroxide-propolis on the number of macrophage cells in the teeth of Wistar rats with pulp perforation. Methods: This study used 30 samples of Wistar rats which were preparate until perforation and then divided into 3 groups. The first group continued with filling wit...
F1000Research, 2020
Background: Caries in the dental pulp result in inflammation and damage to the pulp tissue. During inflammation of the pulp, various inflammatory mediators and growth factors are released, including IL-8, IL-10, TLR-2, VEGF and TGF-β through the NF-kB pathway. In the present study, therapy for pulpal caries was performed through pulp capping by giving a combination of propolis and calcium hydroxide (Ca(OH)2). This treatment was expected to stimulate the formation of reparative dentin as an anti-inflammatory material to prevent pulp tissue damage. Methods: 28 Wistar rats were divided into four groups and treated with Ca(OH)2 with or without the addition of propolis for either 7 or 14 days. Immunohistochemical examination was used to determine the expression of IL-8, IL-10, TLR-2, VEGF, TGF-β in the four treatment groups. Results: The group treated with a combination of propolis and Ca(OH)2 for 7 days showed that the expression of IL-10, IL-8, TLR-2, VEGF, TGF-β increased significantl...
Conservative Dentistry Journal
Background: Pulp inflammation can be treated with pulp capping by calcium hydroxide as the gold standard. Pulp capping by calcium hydroxide could causing tissue necrosis followed by inflammation. Calcium hydroxide combined with propolis which have anti-inflammatory effects through inhibition of the Nf-kB pathway and pro-inflammatory cytokines. That process will inhibit the increase of vascular permeability and neutrophil chemotaxis. Inflammatory process can accelerate by controlling the amount of neutrophil cells as a acute phase marker, so the healing process occurs more quickly. Therefore it is necessary to study the amount of neutrophil cells in the pulp perforation of Wistar rats after applicated by combination of calcium hydroxide-propolis. Purpose: To analyze the difference amount of neutrophil cells on the Wistar rats pulp perforation after applicated by calcium hydroxide-propolis combination compared with calcium hydroxide-aquadest Method: M1 tooth perforated for all groups ...
Conservative Dentistry Journal
Background: The vitality of the pulp must be protected because the pulp contains fibers, cells, and various structures. Pulp vitality can be maintained by pulp capping to stimulate the formation of reparative dentin. The material commonly used for pulp capping is calcium hydroxide. Calcium hydroxide has the disadvantage of causing inflammation and necrosis of the pulp surface. To compensate that, propolis is added because it has the ability as an anti-inflammatory. Purpose: To analyze the increase in the number of fibroblast cells and angiogenesis in the pulp after the application of combination of Calcium hydroxide-propolis compared to the application of Calcium hydroxide.. Methods: This study used 30 samples of wistar rats which were preparated until perforation and then divided into 3 groups. The first group continued with filling with cention, the second group continued with application of calcium hydroxide and filled with cention, and the third group continued with the applica...
2021
Exposed pulp will produce pro-inflammatory cytokines, which are the main target in inhibiting inflammation of the pulp. Early reparative dentin formation begins with the synthesis of extracellular matrix dentin such as type I collagen, which is believed to be the key of successful pulp capping treatment. Propolis is an alternative pulp capping material that is known to reduce inflammation in the exposed pulp. Calcium hydroxide (CH) is the gold standard for pulp capping material but many studies have shown some limitations on calcium hydroxide. This study was conducted to determine the expression of TNF-α and ALP after the application of a combination of calcium hydroxide and propolis extract as pulp capping material. The maxillary first molars of sixty rats (Wistar) were mechanically perforated. Teeth were divided into 3 groups containing 20 each. Group I as a control, group II treated with calcium hydroxide, group III treated with calcium hydroxide and propolis. The final restorati...
2019
Rabia Ejaz Yousaf1, Mubasher Rasheed2, Mohammad Moazzam3, Babra Marium Anwari4, Asdaq Hussain5, Sohail Abbas Khan6 1 Department of Operative Dentistry, Azra Naheed Medical and Dental College, Lahore 2 Department of Operative Dentistry, Sharif Medical and Dental College,Lahore 3 Department of Operative Dentistry, Sharif Medical And Dental College,Lahore 4 Incharge Crown and Bridge Department, Punjab Dental Hospital, Lahore 5 Department of Conservative Dentitry and Endodontics, Kathmandu University School of Medical Sciences, Dhulikhel,Kavre,Nepal 6 Principal , Avicenna Medical and Dental College, Lahore
The thickness of odontoblast-like cell layer after induced by propolis extract and calcium hydroxide
Dental Journal (Majalah Kedokteran Gigi), 2016
Background: Propolis is a substance made from resin collected by bees (Apis mellifera) from variety of plants, mixed with its saliva and various enzymes to build a nest. Propolis has potential antimicrobial and antiinflammatory agents with some advantages over calcium hydroxide (Ca(OH)2). Ca(OH)2 has been considered as the “gold standard” of direct pulp-capping materials, but there are still some weakness of its application. First, it can induce pulp inflammation which last up to 3 months. Second, the tissue response to Ca(OH)2 is not always predictable. Third, the tunnel defect can probably formed in dentinal bridge with possible bacterial invasion in that gap. Purpose: This study was aimed to determine and compare the thickness of odontoblast-like cells layer after induced by propolis extract and Ca(OH)2 in rat’s pulp tissue. Method: Class 1 preparation was done in maxillary first molar tooth of wistar mice until the pulp opened. The Ca(OH)2 and propolis extract was applied to ind...
European Journal of Dentistry
Objective To analyze the expression of nuclear factor kappa B (NF-kB) and collagen type 1 on dental pulp after a treated combination of calcium hydroxide and propolis. Materials and Methods The first maxillary molars of 30 rats were mechanically perforated. Teeth were divided into three groups of 10 for two separate extraction time frames, giving a total of 60 rats. The control groups were treated with Cention, the second treatment groups were treated with calcium hydroxide, and the third treatment groups were treated with a combination of calcium hydroxide and propolis. Final restoration was done with Cention. The teeth were extracted on days 7 and 14, and the expression of NF-kB and collagen type I was analyzed using immunohistochemistry. Results There is lowest NF-kB expression and highest collagen type 1 expression on dental pulp after treated with a combination of calcium hydroxide and propolis on days 7 and 14 (p < 0.05). Conclusion The combination of calcium hydroxide and ...
Herbals have been used for centuries to prevent and control disease. Herbal extracts are effective because they interact with specific chemical receptors within the body and are in a pharmacodynamic sense than drugs themselves so usage of herbal extract averte many patients from many side effects that generally come with traditional medicines. Propolis gained a popularity in the field of Dentistry because of its antimicrobial, anti-inflammatory, healing, anesthetic and cariostatic properties. The aim of the current study was to evaluate the histological response of a healthy rabbit pulp to direct pulp capping with propolis compared to calcium hydroxide. Twelve male rabbits were selected, their dental pulps were intentionally exposed by using low speed round bur on labial surfaces of permanent central incisors. Split mouth technique was used for applying the capping material to control and experimental groups. Each group was subdivided into three subgroups, four rabbits for each, where rabbits were sacrificed after 1, 2 and 4 weeks from capping time respectively. Teeth were dissected after animal scarification and prepared for histopathologic and histochemical evaluation using Hematoxylin_ Eosin (HE) and Trichrome stains. The results showed that 25%, 50% and 25% of dental pulps capped with calcium hydroxide showed mild, moderate and severe inflammatory response respectively, while in propolis group 75% and 25% showed slight to moderate inflammatory response respectively. As regard hard tissue formation in response to capping materials, in calcium hydroxide group half of the cases showed moderate deposition and other half showed marked hard tissue deposited at fourth week of follow up period. While in propolis group there was a marked deposition in 75% of cases and moderate deposition in 25% in the other. Conclusion propolis proved to have less intense inflammatory response and better quality dentin bridge formation.
THE INFLAMMATORY REACTION OF PULP TISSUE WITH CALCIUM HYDROXIDE, MTA AND PORTLAND CEMENT (In Vivo)
ODONTO : Dental Journal
Background : Perforation in normal pulp could be happened anytime. In sterile condition, direct pulp capping was the right therapy to maintain the vitality and pulp function. Direct pulp capping agent must contact with the pulp tissue. Generally, body had an immunologic respond with foreign body that contact with tissue. The respond could be inflammatory reaction. The used direct pulp capping agents were calcium hydroxide, and Mineral Trioxide Aggregate (MTA) which known as better agent but relatively expensive and difficult to obtain. So that, the alternative of MTA, Portland cement which was the same essential of MTA, was being developed. The aim of this study, to analized inflammatory reaction of the pulp tissue with direct pulpcapping agents calcium hydroxide, MTA, and Portland cement.Methods: Free caries M. Nemestrina’s posterior teeth were prepared to formpin point perforations on buccal surface of the crowns. The teeth were appliedwith one of the three capping agents. Teeth w...