The Expression of Tumor Necrosis Factor Alpha and Alkaline Phosphatase Due to Induction of Combination Calcium Hydroxides and Propolis (original) (raw)
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F1000Research, 2020
Background: Caries in the dental pulp result in inflammation and damage to the pulp tissue. During inflammation of the pulp, various inflammatory mediators and growth factors are released, including IL-8, IL-10, TLR-2, VEGF and TGF-β through the NF-kB pathway. In the present study, therapy for pulpal caries was performed through pulp capping by giving a combination of propolis and calcium hydroxide (Ca(OH)2). This treatment was expected to stimulate the formation of reparative dentin as an anti-inflammatory material to prevent pulp tissue damage. Methods: 28 Wistar rats were divided into four groups and treated with Ca(OH)2 with or without the addition of propolis for either 7 or 14 days. Immunohistochemical examination was used to determine the expression of IL-8, IL-10, TLR-2, VEGF, TGF-β in the four treatment groups. Results: The group treated with a combination of propolis and Ca(OH)2 for 7 days showed that the expression of IL-10, IL-8, TLR-2, VEGF, TGF-β increased significantl...
2021
Background: Cellular response of dental pulp to existing lesions, caused by infiltration of inflammatory cells that migrate from blood vessels to the site of injury. Macrophages are the cells that most appear when inflammation occurs. Treatment for maintaining pulp tissue vitality is called pulp capping. Calcium hydroxide as a pulp capping material has the ability to trigger the growth of dentin bridges or remineralization, but calcium hydroxide can induce migration and proliferation of inflammatory cells. Currently the field of dentistry is developing propolis as an alternative pulp capping material. That is because propolis has anti-inflammatory properties. Purpose: To analyze the effect of the combination of calcium hydroxide-propolis on the number of macrophage cells in the teeth of Wistar rats with pulp perforation. Methods: This study used 30 samples of Wistar rats which were preparate until perforation and then divided into 3 groups. The first group continued with filling wit...
Conservative Dentistry Journal
Background: Cellular response of dental pulp to existing lesions, caused by infiltration of inflammatory cells that migrate from blood vessels to the site of injury. Macrophages are the cells that most appear when inflammation occurs. Treatment for maintaining pulp tissue vitality is called pulp capping. Calcium hydroxide as a pulp capping material has the ability to trigger the growth of dentin bridges or remineralization, but calcium hydroxide can induce migration and proliferation of inflammatory cells. Currently the field of dentistry is developing propolis as an alternative pulp capping material. That is because propolis has anti-inflammatory properties. Objective: To analyze the effect of the combination of calcium hydroxide-propolis on the number of macrophage cells in the teeth of wistar rats with pulp perforation. Method: This study used 30 samples of Wistar rats which were preparated until perforation and then divided into 3 groups. The first group continued with filling w...
European Journal of Dentistry
Objective To analyze the expression of nuclear factor kappa B (NF-kB) and collagen type 1 on dental pulp after a treated combination of calcium hydroxide and propolis. Materials and Methods The first maxillary molars of 30 rats were mechanically perforated. Teeth were divided into three groups of 10 for two separate extraction time frames, giving a total of 60 rats. The control groups were treated with Cention, the second treatment groups were treated with calcium hydroxide, and the third treatment groups were treated with a combination of calcium hydroxide and propolis. Final restoration was done with Cention. The teeth were extracted on days 7 and 14, and the expression of NF-kB and collagen type I was analyzed using immunohistochemistry. Results There is lowest NF-kB expression and highest collagen type 1 expression on dental pulp after treated with a combination of calcium hydroxide and propolis on days 7 and 14 (p < 0.05). Conclusion The combination of calcium hydroxide and ...
Conservative Dentistry Journal
Background: Pulp inflammation can be treated with pulp capping by calcium hydroxide as the gold standard. Pulp capping by calcium hydroxide could causing tissue necrosis followed by inflammation. Calcium hydroxide combined with propolis which have anti-inflammatory effects through inhibition of the Nf-kB pathway and pro-inflammatory cytokines. That process will inhibit the increase of vascular permeability and neutrophil chemotaxis. Inflammatory process can accelerate by controlling the amount of neutrophil cells as a acute phase marker, so the healing process occurs more quickly. Therefore it is necessary to study the amount of neutrophil cells in the pulp perforation of Wistar rats after applicated by combination of calcium hydroxide-propolis. Purpose: To analyze the difference amount of neutrophil cells on the Wistar rats pulp perforation after applicated by calcium hydroxide-propolis combination compared with calcium hydroxide-aquadest Method: M1 tooth perforated for all groups ...
2019
Rabia Ejaz Yousaf1, Mubasher Rasheed2, Mohammad Moazzam3, Babra Marium Anwari4, Asdaq Hussain5, Sohail Abbas Khan6 1 Department of Operative Dentistry, Azra Naheed Medical and Dental College, Lahore 2 Department of Operative Dentistry, Sharif Medical and Dental College,Lahore 3 Department of Operative Dentistry, Sharif Medical And Dental College,Lahore 4 Incharge Crown and Bridge Department, Punjab Dental Hospital, Lahore 5 Department of Conservative Dentitry and Endodontics, Kathmandu University School of Medical Sciences, Dhulikhel,Kavre,Nepal 6 Principal , Avicenna Medical and Dental College, Lahore
Al-Azhar Journal of Dental Science, 2021
Objective: Evaluate histologically and compare the inflammatory pulp response following direct pulp capping of mechanically exposed dogs' teeth with: Ca(OH)2 base Dycal, UltraBlend Plus and TheraCal LC, at different time intervals. Subjects and methods. A total of 54 permanent teeth of six healthy dogs were used. They were randomly divided into three groups (n=18) according to pulp capping material used. Group I: Dycal, Group II: UltraBlend Plus (UBP) and Group III: TheraCal LC. All cavities were restored with Riva LC Resin Modified Glass Ionomer. Each group was subdivided into three subgroups according to storage time (n=6) at time intervals of 1 week, 1 month and 3 months. Control samples (3 teeth) were added representing normal untouched teeth for evaluating histological features of normal pulp. Paraffin sections were prepared stained with H&E then examined under light microscope for histologic evaluation of the inflammatory cell response according to the scoring system sugge...
Open Journal of Stomatology, 2014
Aims: To evaluate the inflammatory response of the exposed pulp of incisor teeth in rats after direct pulp capping, using platelet rich plasma (PRP), enamel matrix derivate (EMD), mineral trioxide aggregate (MTA) and calcium hydroxide (Ca(OH) 2). Methods: The study was conducted on 36 Wistar albino rats with a total of 144 incisor teeth. The pulps of 96 teeth of the rats were perforated and capped with different agents. Serving as the positive control group, the pulps of 24 teeth were only perforated and capped without capping agents, whereas the pulps of 24 teeth were used as the negative control group without being perforated (without any process). The research was ended with the extracting of the teeth on the 7th day-28th day. The teeth were taken to the routine and histological follows; cross sections were prepared and painted with hematoxylen & eosin. All of the sections were evaluated in terms of inflammatory reaction by histologic analysis taken by light microscope. Statistical analysis was used. The normal distribution of all data was tested with the Mann Whitney U and the differences between the groups were analyze dusing Kruskal Wallis test at 0.05 level. Results: There are no statistically significant differences in terms of inflammation type and necrosis among the treatment groups on 7 days' post capping. However, improved inflammatory cell accumulation, hyperemia and lowest necrosis were observed from the samples treated with PRP (p < 0.05). Conversely, the EMD group indicated that the criteria of inflammation scores and hyper-emia were higher in the 28th day (p < 0.05). Conclusions: Most of cells accumulating in the PRP group and most necrosis were seen in the EMD group. These new PRP materials might serve as pulp capping biomaterials to induce initial healing response in the future.
2021
Objectives Direct pulp capping may result in formation of a dentinal bridge and preservation of pulp vitality. This randomized controlled clinical trial sought to histologically assess and compare pulp tissue following pulp capping with propolis and calcium hydroxide. Methods In A cavity was prepared at the center of the occlusal surface of 10 third molars scheduled for extraction by using a cylindrical bur. The pulp chamber was exposed with a round bur. Samples were randomly divided into two groups (5 teeth in each group). The first group underwent direct pulp capping with propolis and the second group with calcium hydroxide. Auto-polymerizing glass ionomer was then applied to seal the cavity. The teeth were extracted after 45 days, and histologically evaluated. The obtained data were analyzed using the Fisher’s exact test. Results The quality (P=0.048) and quantity (P=0.008) of dentinal bridge were significantly different between the two groups. Propolis resulted in formation of a...