Effect of stigmatic exudate and pistil extracts of Cajanus cajan (L.) millsp. on in vitro pollen growth of Cyamopsis tetragonoloba taub (original) (raw)
Related papers
1982
Self fertile pollen of Cyamopsis tetragonoloba showed an increase in the percentage of germination and tube length in vitro when the basal medium consisting of sucrose, calcium, and boron was supplemented with either its own stigmatic exudate or that of Vigna catjung. Although Vigna stigmatic exudate promoted the pollen growth of Cyamopsis in vitro, cross pollination of the stigma of the former with the pollen of the latter results in inhibition. Qualitative similarity in biochemical composition, although quantity of the exudate components varies, may be the reason for the observed promotive effect of Vigna stigmatic exudate in v itro. The failure of germination of Cyamopsis pollen on the stigma of Vigna may be controlled by high concentrations of the stigmatic exudate components of Vigna, besides other possible factors.
Pakistan Journal of Botany
Pollen germination capacity and viability of two species viz., Caesalpinia gellisii (Hook.) Dietr. and Caesalpinia pulcherrima (L.) Swartz. of the family Fabaceae were examined up to 48 weeks. Viability was determined on the basis of germination ability of pollen on sucrose medium with boric acid solution. Fresh pollen were stored at different storage conditions including refrigerator (4°C), freezer (-20°C,-30°C), freeze drier (-60°C), vacuum chamber and organic solvents. Pollen stored at low temperature-60°C showed better germination as compared to pollen stored at 4°C and fresh. C. pulcherrima showed much better germination percentage at-60°C (61.00%) whereas, C. gellisii, showed 30.80% germination after 48 weeks of storage. Pollen stored over silica gel in vacuum of both the species showed good germination as compared to organic solvents. Freezer (-20ºC &-30ºC) and freeze dryer (-60ºC) maintained pollen germination capacity and viability for a longer period as compared to fresh, refrigerated, pollen treated in vacuum over silica gel and organic solvents. Conclusively both low temperature and RH humidity are the most influential factors in controlling germination and viability of pollen grains.
Comunicata Scientiae
The aim of this work was to verify the in vitro germination of pollen grains of Angelonia integerrima L., Campomanesia aurea O. Berg and Sesbania punicea (Cav.) Benth in different culture medium and temperatures. For this purpose, flower buds from which pollen was collected and sprayed on plates containing the three evaluated culture medium: M1 - agar and sucrose; M2 - agar, sucrose and H3BO3; M3 - agar, sucrose, H3BO3, Ca(NO3), MgSO4 and KNO3; and two incubation temperatures (20 °C and 30 °C). Data was subjected to analysis of variance after its transformation to square root and means were compared by Fisher’s test (LSD). For the three species, the temperature of 30 ºC provided the highest percentage of pollen grain germination. For A. integerrima, M1 and M3 promoted the highest germination percentages (40.7 % and 56.5 %, respectively). On the other hand, for C. aurea, M2 provided the highest germination average (43.7 %). At last for S. punicea, M3 was the one that provided the hig...
In vitro POLLEN VIABILITY, GERMINATION AND POLLEN TUBE GROWTH IN SOME
2016
Abstract. In this study in vitro pollen viability and germination in intact pollens and pol-len tube growth in germinated pollens of five common pomegranate cultivars grown in Croatia and Bosnia and Herzegovina was investigated first time. Pollen viability varied from 36.73 % (cv. Konjski Zub) to 51.80 % (cv. Barski) in fluorescent diacetat (FDA) test. The average pollen germination percentages were found as the lowest 6.83 % in cv. Kon-jski Zub and the highest 42.51 % in cv Glavas. Among the germination media in general 0.2 agar + 10 % sucrose+5 ppm H3BO3 gave better results to obtain higher pollen germina-tion for all cultivars. In germinated pollens of these five cultivars, significant differences were observed on pollen tube length. Average pollen tube lengths in germinated pollens of five cultivars were measured as minimum 302 μm in cv. Konjski Zub and as maximum 344 μm in cv. Ciparski. The results showed that there were obvious differences in pollen germinability among cultiva...
In vitro pollen germination ijgpb 2013
In vitro pollen germination is the most widely used technique for testing the viability of pollen grains in breeding programs . The cultivated pigeonpea is endowed with a wealth of wild species in the genus Cajanus which includes 32 species . However, only 12 wild species produced hybrids with pigeonpea .Presence of strong incongruity barriers prevents the realization of hybrids with pigeonpea [4]. In vitro pollination/ fertilization is one of the method of overcoming crossability barriers for which in vitro pollen germination medium is a prerequisite.
The most important goal of fruit growers is obtaining high quantity and quality yield in fruit industry which depends on sufficient fruit setting. Pollination and fertilization are the basic factors which affect fruit setting volume. Commercial fruit trees in Iran belong to stone and pome fruits of Rosaceae family with gametophytic self-incompatibility, making necessary the selection of favorable pollinizer in breeding and orchard planning programs. Therefore, knowledge about pollen traits of the species and cultivars in this family is one of the main issues for growers and breeders. In this research, pollen germination, tube growth and longevity were studied in some cultivars and selected favorable genotypes of three species including; apple, pear and quince. Pollen traits were investigated after 35 days maintenance in 0°C, using in vitro medium containing 16% sucrose, 4 ppm acid boric and 1% agar. Cultured pollens were incubated in dark condition at 25°C for 24 h and then, pollen ...
Study of pollen germination in pome fruit tree of Rosaceae family in vitro
Pollination and fertilization are the basic factors affecting fruit setting volume and pollination is an essential component in fruit tree cultivation. The most important goal for fruit growers is obtaining high quantities and qualities yield in fruit industry which depends on sufficient fruit setting. Commercial fruit trees in Iran belong to stone and pome fruits of Rosaceae family with gametophytic self-incompatibility make necessary the selection of favorable pollinizer in breeding and orchard planning programs. Therefore, knowledge about pollen traits of the species and cultivars in this family is one of the main issues for growers and breeders. In this research, pollen germination and tube growth were studied in some cultivars and selected favorable genotypes of 6 genus including; apple, pear loquat, medlar, red hawthorn and quince. Pollen traits were investigated using in vitro medium containing 17% sucrose, 10 ppm acid boric and 1.2% agar. Cultured pollens were incubated in d...
The pollen tubes are considered as the most rapidly growing cells in the plant world since they are capable of attaining considerable length in a short duration under optimum conditions. Present study was carried out to determine in vitro pollen viability and pollen germination in some plants of the family Rosaceae. Pollen viability test, using Acetocarmine and IKI (iodine potassium iodide) stains were used. Pollen germination traits of genotypes were studied using an in vitro medium containing 0%, 5%, 10%, and 20% sucrose to evaluate the best sucrose concentrations for germination. The germinated pollen was counted every after one hour 1, 2, and 3 hour. Among Rosaceous taxa Rosa shows maximum pollen viability percentage in both Acetocarmine and IKI stains, while among miscellaneous taxa Hamelia shows maximum pollen viability. The maximum pollen germination percentage was found in 10% sucrose concentration media in all plants and the maximum pollen tube growth was found in Hamelia patens (394µm) in 20 % sucrose concentration.
ijprb.com
Loquat (Eriobotrya japonica Lindl.) is one of the most perspective trees with tasty fruits suitable for commercial production in Iran. However, self-incompatibility of most loquat cultivars makes it necessary to select good pollinizers in breeding and orchard establishment programs. Therefore, studies on pollen viability traits of cultivars and genotypes have been one of the main issues for loquat growers and breeders which are investigated in this study. The experiment was conducted in in vitro conditions for the identification of the pollen germination and longevity in some loquat genotypes for recognition of the best pollinizers. Pollens of twenty (20) genotypes were gathered and after three weeks storage in 0°C, their pollen germination, pollen tube growth rate and pollen longevity were tested in the in vitro medium. The experiment was carried out based on completely randomized design (CRD) and data were analyzed with SAS software. Significant differences were observed among the genotypes in pollen germination, pollen tube growth rate and pollen longevity. Finally, the best genotypes were selected for using in loquat orchard establishment and breeding programs in Iran.