Sensors for bioreactor monitoring and control - a perspective (original) (raw)
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Analytica Chimica Acta, 1995
The development of a highly flexible sequential-injection system for the on-line control of fermentation processes is described. The developed system shows a significantly improved flexibility and adaptability to the needs of a particular fermentation process to be monitored as compared with conventional flow-injection systems. The number of necessary hardware components has also been drastically decreased, and the minimal injection volume decreased to 3-5 ~1 allowing improved dilution of an injected sample plug and improved mixing of coinjected reagents. A dilution factor can be adjusted to between 1 and 100 due to controlled dispersion of the injected sample plug within the tube system by choosing an appropriate injection volume. Due to the symmetrical design of the injection manifold and the versatile control software, complex injection profiles can be obtained that are necessary to coinject a sample together with a reagent or a sample together with one or two standard solutions for immediate recalibration. The system is fully automated, and the specially developed software package comprises automatic calibration and recalibration with up to 5 standard solutions, real-time calculation of the actual concentration values based on the most recent calibration data, documentation of the fermentation course, and injection of samples from up to 4 sampling sites with variable time basis. 0003-2670/95/$09.50 0 1995 Elsevier Science B.V. All rights reserved SSDI 0003-2670(95)00300-Z 114 W. Schuhmann et al./Analytica Chimica Acta 315 (1995) 113-122
A versatile data acquisition system for physiological modelling of laboratory fermentation processes
Biotechnology Techniques, 1994
A versatile data acquisition system for physiological modelling of laboratory fermentation processes is given. This system meets the stringent qualifications of modem modelling techniques in terms of signal to noise ratios and sampling rates using readily available equipment. The data are portable to any data processing system for modelling and analysing the culture characteristics. Two completely equipped fermentation systems are sampled with a sampling period of six seconds. The data acquisition system is used to acquire data of a set of continuous fermenters showing sustained oscillations. Oscillation data was used to show the performance of the data acquisition system. With the high sampling rate signal to noise ratios of 30 dB or higher are achieved. It was possible to determine periods of oscillatory behaviour and delay times. The system has shown to be versatile in a laboratory set up. Addition or subtraction of sensors or complete fermentation systems can be done without major alterations to the system.
Automatic fermentation control based on a real-time in situ SIRE® biosensor regulated glucose feed
Biosensors and Bioelectronics, 2006
Monitoring and regulation of fermentations is of a paramount industrial and academic importance in order to keep conditions optimal during the entire process. Established techniques employed today include HPLC and spectrophotometry, which both have the disadvantage that broth samples have to be drawn from the fermentor and that they often require sample pre-treatment. The objectives of this study was to design and evaluate a software controlled automatic real-time SIRE ® biosensor connected to a glucose feed solution pump for in situ based monitoring and regulation of the glucose concentration during a yeast fermentation process.
A modular computer-controlled fermentation pilot-plant
Process Biochemistry, 1991
A computer-controlled pilot-plant for the fermentation of both microorganisms and animal cells was designed and set up jbr the scaleup of processes and the production of sample material for clinical trials. The modular design of the fermentation units allows ji)r the adaption to the specljic requirements of production organisms. Extensive documentation of batches, as required by regulatory authorities are achieved by the direct digital control concept with an industriul process control computer system. The pilot-plant hardware is presented and the development of the process control software described. Corresponding author : Dr. 0. Doblhoff-Dier. Telephone : (043-l) 3692924-402; Fax : (043-I) 3692924-400. l Fermentation of both microorganisms and animal or insect cells. 201 Process Biochemistry 0032-9592/91 /S3.50 Q 1991 Elsevier Science Publishers Ltd, England.
Biosensing devices for the semi-automated control of dehydrogenase substrates in fermentations
Biosensors, 1989
Flow injection analysis based on immobilized dehydrogenases is adapted to process control. The assay systems developed for the control of special fermentation problems include, prior to enzymatic analysis, continuous sample purification, e.g. by dialysis and electrodialysis, and dilution steps. Examples are given for the determination of glucose in turbid and coloured media, the monitoring of an L-leucine production from the corresponding a-keto acid, and for the parallel glucose and ethanol determination during a baker's yeast fermentation.
Biosensors as Analytical Tools in Food Fermentation Industry
Advances in Experimental Medicine and Biology, 2010
T he food industries need rapid and affordable methods to assure: the quality of products and process control. Bioscnsors, combining a biological recognition element and a sensitive cransducer, are versatile analytical tools that offer advantages all classical analytical methods due to their inherent specificity, selectivity and simplicity. This paper reviews the recent trends in the development and applications ofbioscnsors used in food fermentation industry. focusing on ampcromctric enzymatic and microbial sensors.
Monitoring batch fermentations with an electronic tongue
Journal of Biotechnology, 2003
An electronic tongue comprising 21 potentiometric chemical sensors with pattern recognition tools was used for the rapid off-line monitoring of batch Escherichia coli fermentations. The electronic tongue was capable of monitoring the changes in the media composition as the fermentation progressed, and could correlate this with an increase in biomass. The electronic tongue was also able to monitor the increase in organic acids, especially acetic acid, throughout the fermentation. This technique clearly shows promise as a rapid tool for fermentation monitoring. #
Enzyme and Microbial Technology, 1997
A jlow-injection analysis system was combined with a mass producible, disposable biosensor and was used to monitor glucose concentrations during several microbial fermentations. The biosensor was manufactured using thick film, screen printing technology. Unlike previous devices of this type, the biosensor was designed to make multiple measurements over extended periods rather than to operate as a "one-shot" sensor. One yeast, one lactic acid, and three E. coli bioreactor cultures were tested using either defined or complex media. Resultsffom the sensor were compared with a standard spectrophotometric test kit. In samples containing glucose concentrations within the range of the biosensor and the test kit, good correlations were obtained between the two methods. In addition to glucose, microbial growth and pH were recorded.