Oral biofilm and caries-infiltrant interactions on enamel (original) (raw)
Related papers
In situ effects of restorative materials on dental biofilm and enamel demineralisation
Journal of Dentistry, 2009
Enamel margins immediately adjacent to restorations are susceptible to secondary caries development, due to the possible presence of marginal gaps, porosities and imperfect adaptation of restorative materials. Consequently, the diag-nosis of secondary caries is the main reason given by dentists for replacement of all types of restorations in permanent and primary teeth, 1,2 as a major part of the dental treatment provided to patients in a general dental practice. 2 Limited durability of dental restorations makes the restorations larger, increasing complexity of the required therapy. Hence, j o u r n a l o f d e n t i s t r y 3 7 ( 2 0 0 9 ) 4 4 -5 1 Resin Glass ionomer Caries prevention Secondary caries a b s t r a c t Objectives: Since secondary caries is one of the main reasons for replacing restorations, this study assessed the effects of different restorative materials on the microbiological composition of dental biofilm and on enamel demineralisation around the restoration.
Oral biofilm formation and retention on commonly used dental materials: an update
Tropical Dental Journal, 2018
Bacterial plaque is a biofilm composed of microbes, organic and inorganic components attached to the soft and hard tissues of oral cavity. Dental plaque is the primary cause of periodontal diseases including gingivitis and periodontitis, dental caries, peri-implantitis, and stomatitis. From an ecological viewpoint, the oral cavity undergoes a continuous introduction and removal of both microorganisms and nutrients. In order to survive in oral cavity, bacteria need to adhere either to the soft or hard tissues in order to resist disruption forces. Past 60 years of literature was searched with key words of plaque, plaque formation, biofilm, material properties and plaque formation, composites, amalgam, glass ionomer, ceramics and dental implants. Dental materials properties were reviewed in relation to biofilm adherence. A very briefly, mechanical and chemical methods of plaque control were reviewed. Recent developments of bioactive restorative materials were reviewed for their surface characteristics and its influence on biofilm formation. It is hoped that dental professionals would find this narrative review helpful for their routine clinical practice to render, ecological friendly restorative dental treatments and material choices to improve the quality of life of their relatively oral hygiene compliant patients' population. Résumé Formation et rétention du biofilm oral sur des matériaux
Biofilm formation on dental materials
Acta stomatologica Naissi
Introduction:Dental materials play a role of morphological and functional substituent of the oral environment and they are expected to perform their function without causing adverse effects. A biofilm is formed on the surface of dental materials, as well as on other oral tissues. Considering that dental materials due to their structure, in most cases, allow accumulation of food residues and infectious content, a comparative review of possible consequences and the way of their prevention is of great importance. The aim of this manuscript was the analysis of biofilm formation on different dental materials surfaces based on published investigations and literature data. Knowing the structure of dental materials and their behavior in oral environment is a base for proper setting of indication for their use. The simplest way to control biofilm formation on materials is good oral hygiene and maintaining dentures. Conclusion:The formation of biofilm in dental materials lead to development of same deseases of oral cavity.The simplest way to control the development of biofilm is to maintain a ligh lovel of oral higiene.
Effect of three methods for cleaning dentures on biofilms formed in vitro on acrylic resin
Journal of …, 2009
The aim of this study was to evaluate the effect of three denture hygiene methods against different microbial biofilms formed on acrylic resin specimens. Materials and methods: The set (sterile stainless steel basket and specimens) was contaminated (37 • C for 48 hours) by a microbial inoculum with 10 6 colony-forming units (CFU)/ml (standard strains: Staphylococcus aureus, Streptococcus mutans, Escherichia coli, Candida albicans, Pseudomonas aeruginosa, and Enterococcus faecalis; field strains: S. mutans, C. albicans, C. glabrata, and C. tropicalis). After inoculation, specimens were cleansed by the following methods: (1) chemical: immersion in an alkaline peroxide solution (Bonyplus tablets) for 5 minutes; (2) mechanical: brushing with a dentifrice for removable prostheses (Dentu Creme) for 20 seconds; and (3) a combination of chemical and mechanical methods. Specimens were applied onto a Petri plate with appropriate culture medium for 10 minutes. Afterward, the specimens were removed and the plates incubated at 37 • C for 48 hours. Results: Chemical, mechanical, and combination methods showed no significant difference in the reduction of CFU for S. aureus, S. mutans (ATCC and field strain), and P. aeruginosa. Mechanical and combination methods were similar and more effective than the chemical method for E. faecalis, C. albicans (ATCC and field strain), and C. glabrata. The combination method was better than the chemical method for E. coli and C. tropicalis, and the mechanical method showed intermediate results.
Egyptian Dental Journal, 2022
Aim: this study aimed to assess surface microhardness of Enamel ProVarnish (Amorphous calcium phosphate (ACP)* (Premier Dental Products, PA, USA) containing fluoride varnish) and Icon resin infiltration (Icon-Infiltrate) ** (Resin Infiltration Icon DMG, Hamburg, Germany) after remineralization of artificial enamel lesion of extracted primary anterior teeth Materials and Methods: eighty primary anterior teeth, which were randomly assignment into two equal groups: Group A and Group B (n=40 per group). Each group was further divided into two subgroups (n=20 per subgroup) according to sealant type: Group I: Enamel ProVarnish (control) and Group II: Icon Resin Infiltrate (intervention). In each group Microhardness test and Streptococcus Mutans adhesion were evaluated. Results: Group II Icon resin infiltrate showed significantly higher microhardness and bacterial adhesion than Group I Enamel ProVarnish (p < 0.05). Conclusion: Icon resin infiltrate showed significantly superior performance than Enamel ProVarnish in surface Microhardness while Enamel ProVarnish presented significant difference than Icon Resin infiltrate in Streptococcus Mutans adhesion.
Analyses of biofilms accumulated on dental restorative materials
American journal of dentistry, 2009
To qualitatively and quantitatively assess the architectural arrangement of microorganisms in biofilm developed on the surface of different restorative materials: ceramic (C), resin composite (RC), conventional (CGIC) and resin-modified glass-ionomer cements (RMGIC). Streptococcus mutans was used to develop a biofilm that adhered to the surfaces of the selected material disks in 30 days. The specimens were stained and analyzed by confocal laser scanning microscopy and COMSTAT. Among biofilm properties, mean thickness, total bio-volume, roughness coefficient and surface-to-volume ratio were investigated, as well as characteristics of the distribution and architecture of viable/nonviable cells in the biofilm. Only the mean biofilm thickness was statistically significantly different among the restorative materials tested. C and RC accumulated the thickest biofilms. Qualitative analysis showed cellular aggregates and fluid-filled channels penetrating to a considerable depth of the biofi...
Biodegradation of Resin-Dentin Interfaces Increases Bacterial Microleakage
Journal of Dental Research, 2010
Bis-GMA-containing resin composites and adhesives undergo biodegradation by human-saliva-derived esterases, yielding Bis-hydroxy-propoxy-phenyl-propane (Bis-HPPP). The hypothesis of this study is that the exposure of dental restorations to saliva-like esterase activities accelerates marginal bacterial microleakage. Resin composites (Scotchbond, Z250, 3M) bonded to human dentin were incubated in either buffer or dual-esterase media (pseudocholinesterase/cholesterol-esterase; PCE+CE), with activity levels simulating those of human saliva, for up to 90 days. Incubation solutions were analyzed for Bis-HPPP by high-performance liquid chromatography. Post-incubation, specimens were suspended in a chemostat-based biofilm fermentor cultivating Streptococcus mutans NG8, a primary species associated with dental caries, for 7 days. Bacterial microleakage was assessed by confocal laser scanning microscopy. Bis-HPPP production and depth and spatial volume of bacterial cell penetration within the...
Dental Materials Journal, 2015
The aim of this study was to evaluate and compare the effects of resin infiltration and sealant type on enamel surface properties and Streptococcus mutans adhesion to artificial enamel lesions. Artificial enamel lesions were produced on the surfaces of 120 enamel specimens, which were divided into two groups: Group A and Group B (n=60 per group). Each group was further divided into four subgroups (n=15 per subgroup) according to sealant type: Group I-Demineralized enamel (control); Group II-Enamel Pro Varnish; Group III-ExciTE F; and Group IV-Icon. In Group A, hardness and surface roughness were evaluated; in Group B, bacterial adhesion was evaluated. Icon application resulted in significantly lower surface roughness and higher hardness than the other subgroups in Group A. In Group B, Enamel Pro Varnish resulted in lowest bacterial adhesion, followed by Icon. This study showed that resin infiltration of enamel lesions could arrest lesion progress.