Investigation of Hepatoprotective and Antioxidant Activity of Celosia argentea against Tissue Injury Caused by Rifampicin Administration (original) (raw)
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In vitro and in vivo antioxidant activities of the aqueous extract of Celosia argentea leaves
Indian Journal of Pharmacology, 2011
Strychnos henningsii Gilg is used traditionally for the treatment of various ailments in southern Africa traditional medicine. The antioxidant and free radical scavenging activity of aqueous extract of this plant was investigated both in -vivo and -vitro using spectroscopic method against 1,1-diphenyl-2picrylhydrazyl (DPPH), superoxide anions, hydrogen peroxide (H 2 O 2 ), nitric oxide (NO), 2,2'-azinobis [3-ethylbenzothiazoline-6-sulfonic acid] diammonium salt (ABTS) and the ferric reducing agent. Total phenols, flavonoid, flavonol and proanthocyanidin were also determined to assess their effects on the antioxidant activity of this plant. Free radical scavenging activity of the plant extract against H 2 O 2 , ABTS and NO was concentration dependent with IC 50 value of 0.023, 0.089 and 0.49 mg/ml respectively. However, S. henningsii exhibited lower inhibitory activity against DPPH with IC 50 value of 0.739 mg/ml. The reducing power of the extract was found to be concentration dependent. The administration of the aqueous extract at 250, 500 and 1000 mg/kg body weight to male Wistar rats significantly increased the percentage inhibition of reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT). Whereas, lipid peroxidation level in hepatotoxic rats decreased significantly at the dose of 500 and 1000 mg/kg body weight at the end of 7 days. The extract yielded high phenol content (48 mg/g tannic acid equivalent) followed by proanthocyanidin (8.7 mg/g catechin equivalent) flavonol (5.5 mg/g quercetin equivalent) and flavonoids (4.8 mg/g quercetin equivalent) respectively. A positive linear correlation was observed between these polyphenols and the free radical scavenging activities.
The present study was carried out to compare the secondary metabolites, in vitro and in vivo antioxidant activities as well as the safety of ethyl acetate and methanolic extracts of Celosia argentea leaves in cadmium-induced oxidative stress in rats. The secondary metabolite screening was done by standard methods while the in vitro antioxidant activity of the extract was evaluated using ammonium thiocyanate, reducing power and diphenyl picryl hydrazyl (DPPH) radical scavenging models. In the in-vivo antioxidant and toxicological studies, thirty rats (Rattus novergicus) weighing 137.05 ± 5.84g were completely randomized into six groups (A-F) of five animals each. Animals in group A received orally 0.5ml of distilled water for 7 days while those in groups B, C, D, E and F received same volume corresponding to 8 mg/kg body weight (bw) of cadmium, in addition to simultaneous administration of distilled water, 100 mg/kg b.w of ascorbic acid, 100, 200 and 400 mg/kg b.w. of the extract respectively. Biochemical indices of in vivo antioxidant activities and toxicity were evaluated in the animals after the treatment period. The ethyl acetate extract of C. argentea contained saponins (1.67%), tannins (0.65%), cardenolide and dienolides (1.20%) and phenolics (0.42%) whereas the methanolic extract contained saponins (3.20%), tannins (0.65%), cardenolide and dienolides (0.006%) and phenolics (5.72%). Reducing sugar, steroids, and glycosides were only detected in the ethylacetate extract. The ethyl acetate extract and ascorbic acid, at 50 mg/ml, inhibited linoleic acid oxidation by 51.00 and 24.2% respectively whereas the methanolic extract produced 51.01% inhibition. Ethylacetate extract at 10, 50 and 100 mg/ml produced reducing power of 0.116, 0.092 and 0.127 nm whereas the methanolic extract produced 0.131, 0.185 and 0.183nm when compared with ascorbic acid that gave 0.092, 0.089 and 0.107 nm. The 100 μg/ml of both the ethyl acetate and methanolic extracts scavenged 82% and 30% respectively of the DPPH radical as against 65% in ascorbic acid. Both the extracts attenuated the cadmium chloride treatment related reduction in the activities of superoxide dismutase, catalase, alkaline phosphatase, gamma glutamyl transferase, alanine and aspartate transaminase as well as the levels of uric acid, albumin, total and conjugated bilirubin, total protein and the Cd elevated levels of malondialdehyde in the serum and tissues of the animals in a manner similar to that of the ascorbic acid treated animals and the non-Cd treated animals administered distilled water; with the ethyl acetate producing a better result. The totality of the results conferred antioxidant activity on the ethyl acetate extract and methanolic extract by the phenolic components of the extracts via induction of the antioxidant enzymes and scavenging of free radical. The extracts also reversed cadmium induced changes in the biomarkers of liver damage.
Free Radical Scavenging and Cytotoxic Potential of Celosia argentea
Pharmacognosy Journal, 2015
Introduction: Oxidative stress due to reactive oxygen species often leads to pathogenesis of chronic diseases such as cancer. Research states that a diet rich in polyphenols renders many health benefits by scavenging such harmful reactive species. Celosia argentea (Amaranthaceae), a common weed in India has been reported as a potential source of cheap, natural antioxidants due to its phenolic abundance. In this research work efforts were made to identify and screen the phenolic rich fractions of Celosia argentea for their antioxidant and anticancer potential. Materials and Methods: Various solvent fractions with increasing polarity were subjected to total phenolic content, followed by antioxidant assays-DPPH, ABTS and anti proliferative assays-Brine shrimp Bioassay, Antimitotic and MTT assays. Results: IC 50 value of methanolic fraction for DPPH assay was statistically significant (26.25; ***P<0.001) when compared with ascorbic acid (12.50; ***P<0.001). Also TEAC values for methanolic fraction and BHT (standard) for ABTS assay were similar (2.1; ***P<0.001) Methanolicfraction at 400 µg/ml exhibited strong cytotoxicity (9.0 ± 0.81; ***P<0.001) against brine shrimps comparable to Methotrexate at 50 µg/ml(10; ***P<0.001) and significantly reduced mitotic index from 96.8 to 38.0 (***P<0.001) which was further confirmed by MTT assay where IC 50 value of methanolic fraction for SiHa and MCF-7 cells was found to be 28 µg/ml with no cytotoxicity to normal cells proving its anticancer potential. Conclusion: This research proves antioxidant and anticancer potential of phenolic rich fraction of Celosia argentea and suggests it to be useful in cancer management as antifroliferative, chemo preventive and in cancer chemotherapy induced immune suppression and oxidative stress.
Pharmaceutical and Biomedical Research, 2019
Several human diseases including cancer, rheumatoid arthritis, and neurodegenerative and hepatic diseases are related to the destructive effect of reactive oxygen species (ROS). Antioxidants may provide a possible solution to this problem. This study was carried out to investigate the effect of hydroalcoholic extract of Celtis australis on CCl4 induced hepatotoxicity in mice. The antioxidant activity of C. australis was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. For evaluation of hepatoprotective activity of the extract, the animals were pretreated with 200 and 400 mg/kg of C. australis extract intraperitoneally for 7 days and then received CCl4 (0.5 ml/kg in olive oil). Liver injury was determined by serum biochemical parameters such as Alanine aminotransferase (ALT), Aspartate aminotransferase (AST), Alkaline phosphatase (ALP), glutathione contents of liver tissue, and histopathological studies. A significant reduction in the serum biochemical para...
Fronteiras: Journal of Social, Technological and Environmental Science
The objective of this work was to evaluate the chemoprotective effects of the embaúba Cecropia distachya Huber (two methanolic fractions: F1 and F2) on oxidative stress induced by cyclophosphamide (75 mg kg-1) in mice, as well as phytochemical analyzes by LC-MS/MS. In the experimental model, the fractions increased the hepatic and cardiac catalase (CAT), reduced glutathione (GSH) of the kidney and the heart. F1 increased platelet levels (PLT), hemogram (RBC), hematocrit (HCT) and hemoglobin (Hb) and F1 reduced hepatic lipid peroxidation (TBARS) and aspartate aminotransferase (AST) activity increased. F1 was attributed to possible hypoglycemic activity and possible immunosuppressive effects and F2 presented antimutagenic activity. In some cases, the fractions were also hepatotoxic. These results demonstrated that the fractions stimulate the antioxidant defenses, being hypoglycemic (F1) and antimutagenic (F2), and, the harmful effects attributed to the fractions may be the association...
Comparative Clinical Pathology, 2018
Celosia argentea commonly known as cockscomb plant is widely used in folkloric medicine in the treatment and management of diabetes mellitus. The effect of methanolic extract of Celosia argentea var. cristata L. (CAVCL) leaves on blood glucose level, superoxide dismutase (SOD), catalase (CAT), alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) activities, and malondialdehyde (MDA) level were evaluated in diabetic rats. Five groups of male albino rats consisting of 5 animals each were used for the present study. They were grouped as normal control, diabetic control, diabetic administered with 250 and 750 mg/kg b.w C. argentea, and 5 mg/kg b.w glibenclamide. Diabetes was induced with alloxan monohydrate intraperitoneally at 120 mg/kg b.w. The control and diabetic groups were given distilled water and rat chow for 21 days. Blood glucose level of each group was estimated every week, and at the end of the experiment, SOD, CAT, MDA and serum ALP, and AST and ALT activities were assayed in the liver and serum respectively of the experimental animals. The results showed a significant increase (p < 0.05) in serum AST, ALP, and ALT activities and reduction in SOD and CAT activities compared with normal control groups. The extract at both doses significantly lowered the high levels of the serum enzymes and increased the level of CAT and SOD. These results indicate an anti-hyperglycemia and antioxidative protective effect of C. argentea leaves.
Food and Chemical Toxicology, 2011
Ceratonia siliqua is a typical Mediterranean plant, mainly used in food and Tunisian traditional folk medicine. Among the tested extracts, the ethyl acetate fraction (EACs) exhibited the highest total phenolic and flavonoids content. The antioxidant activity in vitro systems showed a more significant potent free radical scavenging activity of this extract than other analysis fractions. The HPLC finger print of EACs active extract showed the presence of six phenolic compounds. The in vivo results showed that oral administration of CCl 4 enhanced levels of hepatic and renal markers (ALT, AST, ALP, LDH, c-GT, urea and creatinine) in the serum of experimental animals. It also increased the oxidative stress markers resulting in increased levels of the lipid peroxidation with a concomitant decrease in the levels of enzymatic antioxidants (SOD, CAT, GPx) in both liver and kidney. The pre-treatment of experimental rats with 250 mg/kg (BW) of the EACs, by intraperitoneal injection for 8 days, prevented CCl 4 induced disorders in the levels of hepatic and kidney markers. The biochemical changes were in accordance with histopathological observations suggesting a marked hepatoprotective and nephroprotective effect of the EACs extract.
Phytochemical analysis and antimicrobial and antioxidant activities of Celosia argentea leaves
Scientia Plena
This study performed the phytochemical screening and bioactivity of Celosia argentea leaves. The phytochemical profile of hexane (Hex), ethyl acetate (AcOEt) and methanolic (MeOH) extracts of C. argentea leaves was observed by Thin Layer Chromatography and spectrophotometric analysis. In the susceptibility test, the disk-diffusion technique was performed. Minimum Inhibitory Concentration (MIC) was assessed by microdilution. Minimum Microbic Concentration (MMC) was determined in the extracts which presented MIC. Antioxidant activities were measured using 2,2-diphenyl-1-picril-hidrazil (DPPH), determination of reducing power and total antioxidant capacity (TAC). The results showed a higher content of phenolic compounds (252.02 ± 0.02 mg GAE/g) in the extract AcOEt, and of tannins (103.72 ± 0.004 mg/EAT/g) in MeOH; this was effective against Micrococcus luteus (inhibition of 24.7 ± 0.6 mm). MIC and CMM of the AcOEt for M. luteus were 0.06 and 0.25 mg/mL, respectively. The MeOH extract ...
Anti-inflammatory and cytotoxic evaluation of extracts from the flowering stage of Celosia argentea
BMC Complementary Medicine and Therapies
Background This study was aimed at investigating the possible anti-inflammatory and cytotoxic effects of extracts from the flowering stage of C. argentea. This growth stage was chosen because of its high polyphenolic content and high antioxidant capacity. Methods Anti-inflammatory potential of the aqueous, acetone and methanol extracts of C. argentea was evaluated through the inhibition of nitric oxide production (LPS-induced) on stimulated macrophages (RAW 264.7), while MTT assay was used to assess cell viability with Silymarin as standard. Cytotoxicity of the plant extracts was evaluated on murine preadipocyte cell line (3 T3-L1) using the image-based method of two DNA-binding dyes; Hoechst 33342 and propidium iodide (PI) with melphalan as standard. Results Acetone extract exhibited moderate, dose-dependent anti-inflammatory activity with no significant toxicity to activated macrophages, however the aqueous and methanol extracts were unable to inhibit nitric oxide production at bo...
GSC Biological and Pharmaceutical Sciences, 2019
This study is designed to investigate the inhibitory effect of Celosia trigyna against xanthine oxidase. Total flavonoid, phenol, vitamin C were evaluated using standard methods and inhibitory activity of Celosia trigyna extract on xanthine oxidase (XO) activity in vitro and chelating properties of extract against Iron (II) were also carried out. The results showed that the extract contained high content of flavonoids, phenolic acid and vitamin C that are beneficial to health. The extract inhibited XO in a dose-dependent manner. The extract also chelated Fe2+ in a dose-dependent manner. Celosia trigyna extract could be a promising nutraceutical for preventing and managing hyperuricaemia due to its ability to inhibit XO; this activity is attributed to the combined effect of its phytochemicals and chelating properties.