The effect of low level laser (GaAlAs) therapy on the post-surgical healing of full thickness wounds in rabbits (original) (raw)
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The Effect of Low Level Laser Therapy on Surgical Wound Healing
2010
Surgical wounds are superficial or deep wounds on skin or mucous membranes due to a surgical intervention using a scalpel to cut through skin or mucosa and the underlying tissue. The wound treatment costs in terms of finance and time are relatively high; the complete wound healing lasts 3 weeks. To reduce this period of healing of surgical wound and avoid complications, therapeutic methods and drug are used in postoperative period. One of these methods is Low Level Laser Therapy. This method has been used for treatment of wounds for over two decades in many medical facilities of the world. However, despite such wide clinical usage, there is still controversy regarding the efficacy of Low Level Laser Therapy in the treatment of wounds. Many laser systems, different laser parameters and irradiation conditions, and a great variety of treatment protocols lead to these conclusions. The goal of this paper is to present our evaluation of Low Level Laser Therapy role in surgical aseptic wou...
Journal of Applied Oral Science, 2004
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Photomedicine and Laser Surgery, 2010
Objective: The aim of the present investigation is to evaluate the effects of a 780-nm low-level laser on open skin wound healing. Background Data: Optimal parameters of low-level laser therapy (LLLT) for wound healing are discussed. Methods: One full-thickness skin wound was surgically induced in the dorsum skin of 30 rats. The rats were divided into two groups. Rats in the experimental group were daily treated with a gallium aluminum arsenide (GaAlAs) laser (2 J=cm 2 , l ¼ 780 nm, pulse frequency of 2336 Hz). Rats in the sham-exposed group received LLLT with switched off equipment. After 4, 7, and 15 days, wounds were checked by histological and biomechanical methods. Data were analyzed by the Mann-Whitney U-test. Results: Fibroblasts, endothelium of blood vessels, blood vessel sections, and maximum stress were significantly increased, whereas macrophages were significantly decreased, compared with those of the sham-exposed group. Conclusion: Pulsed LLLT with a 780-nm GaAlAs laser significantly accelerates the process of healing of surgically induced, full-thickness skin wounds in rat.
Laboratory methods for evaluating the effect of low level laser therapy (LLLT) in wound healing
African Journal of Biomedical Research, 2006
The basic tenet of laser therapy is that laser radiation has a wavelength dependent capability to alter cellular behaviour in the absence of significant heating. Low intensity radiation can inhibit as well as stimulate cellular activity. Laser therapy typically involves the delivery of 1-4J/cm 2 to treatments sites with lasers having output powers between 10mW and 90mW. There are two major areas of laser therapy research: the laboratory and the clinic. The laboratory presents the least ambiguous results. Here, although unsupported results do appear, the vast majority of published work finds clear evidence that laser irradiation alters cellular processes in a nonthermal, wavelength-dependent manner. Low energy laser irradiation alters t he cellular function by effecting protein synthesis, cell growth and differentiation, cell motility, membrane potential and binding affinities, neurotransmitter release, ATP synthesis and prostaglandin synthesis. Laboratory findings provide scientific rati onale of laser therapy and the effect of laser therapy on cellular processes. This review outlines some of the current methods employed in the laboratory to measure the effect of low level laser therapy (LLLT) on cellular and molecular processes in the cell. This review briefly explains the different structural, cellular and molecular parameters and highlights some of the basic principles and protocols including specialized equipment requirements.
A histological study of the effect of the low level laser therapy on wound healing
In this study we show an approach for wound healing process based on using of low level laser therapy, in order to enhance the healing rate of wounds. The low power laser (LPL) has been introduced also to solve problems associated with scar formation after completing the healing process. In this study the wound healing process rate was enhanced by using the illumination of 650 nm, 5 mW diode laser in CW and 2.5 mW maximum average power pulse modes with 1 MHz frequency and 50% duty cycle. The low power laser light biostimulates the wounded area which was made on the back of many mice. The healing process was histologically studied during fourteen days to find out the differences between the laser illuminated groups of wounds and other group of wounds (control). From the results, we found out that the pulse diode laser is more efficient than continuous diode laser in enhancing the wound healing process within shorter time.
ow-intensity laser therapy aims at pain suppression, edema reduction and acceleration of wound healing. The main goal of this study was to clinically evaluate the effects of Aluminum Gallium Arsenate laser -670nm in wound healing after gingivoplasty in 11 patients. Surgery was performed in anterior superior and/or inferior regions. The right side of the patient (test group) received a laser energy density of 4J/cm², in a 48-hour interval, during one week, totalizing four sessions. The irradiation was punctual in a contact mode in three points. The left side did not receive irradiation (control group). Clinical evaluation was performed by five specialists in periodontology through photography of the treated areas at post-surgical periods of 7, 15, 21,30,60 days. The observers pointed the best healed side. The Sign test was used for statistical analysis with a confidence level of 5% (P<0.05). The examiners found a better pattern of healing sometimes in the test and sometimes in the control group until 21 days after surgery. After this period there was no apparent difference between them. There was no statistical difference between the sides (p>0.05). These results have shown that low-intensity laser therapy did not accelerate oral mucosa healing after gingivoplasty. UNITERMS: Laser; Gingivoplasty; Wound healing. terapia com laser em baixa intensidade visa a biomodulação dos tecidos para se obter supressão da dor, redução do edema, e aceleração da cicatrização. O objetivo deste estudo foi avaliar, clinicamente, os efeitos do laser diodo de arseneto de gálio e alumínio (GaAlAs) -670nm-na cicatrização de gengivoplastias em 11 pacientes. As cirurgias foram realizadas nas regiões anteriores superior e/ou inferior. O lado direito (teste) foi irradiado, na forma pontual, com 4J/cm² por ponto, em três locais diferentes. A aplicação do laser foi feita a cada 48h, durante uma semana, totalizando quatro sessões. O lado esquerdo (controle) não foi irradiado. A avaliação clínica foi feita por cinco periodontistas, através de fotografias dos períodos pósoperatórios de 7,15,21, 30 e 60 dias. Os examinadores apontaram o lado mais bem cicatrizado ou se ambos estavam iguais. Para análise estatística dos dados clínicos, foi utilizado o teste dos sinais com um nível de significância de 5% (p<0,05). Os resultados mostraram que havia preferência ora pelo lado teste, ora pelo lado controle até o período de 21 dias e nos períodos subseqüentes ambos os lados foram considerados iguais. A diferença entre os lados estudados não foi estatisticamente significante, sugerindo que o laser não acelerou a cicatrização da mucosa oral.
Clinical evaluation of low level laser therapy in skin wound healing in maxillofacial surgery
Background: Wound healing is a complicated, interactive, integrative process involving cellular and chemotactic activity, the release of chemical mediators and associated vascular response which includes number of phases: inflammatory phase, proliferative phase and remodeling phase. Low level laser therapy can be more effective in the three overlapping phases of wound healing. Biostimulation appears to have an effect on the cellular level, by increasing cellular function and stimulating various cells. Objective: To evaluate the efficacy of low level diode laser on wound healing of human skin clinically. Patients and Methods: This study was performed on 20 patients (10 male, 10 female) age range between 5-75 years with oral and maxillofacial lesions underwent maxillofacial surgery with low level laser therapy. After the surgical intervention, the wounds were divided into two parts, one part was irradiated by 1.25 W/cm 2
Lasers in Medical Science, 2013
The aim of the present study was to compare the effectiveness of four different laser wavelengths (660, 810, 980, and 1,064 nm) used for low-level laser therapy (LLLT) on the healing of mucositis in an animal model of wound healing by investigating the expression of platelet-derived growth factor (PDGF), transforming growth factor beta (TGF-β), and blood-derived fibroblast growth factor (bFGF). Thirty-five male Wistar albino rats with a weight of 250-300 g body mass and 5 months old were used in the study. All animals were intraperitoneally injected with 100 mg/kg of 5-fluorouracil (5-FU) on the first day and 65 mg/kg of 5-FU on the third day. The tip of an 18-gauge needle was used in order to develop a superficial scratching on the left cheek pouch mucosa by dragging twice in a linear movement on third and fifth days. After ulcerative mucositis were clinically detected on the animals' left cheek pouch mucosa, the laser therapy was started. Four different laser wavelengths (660 nm, HELBO, Bredent; 810 nm, Fotona XD, Fotona; 980 nm, ARC Fox; and 1,064 nm, Fidelis Plus 3, Fotona) used for LLLT at ED 8 J/cm 2 daily from the first to the fourth days. Oval excisional biopsy was taken from the site of the wound, and the expression of PDGF, TGF-β, and bFGF was evaluated. The obtained data were analyzed by one2-way ANOVA, and then Tukey HSD tests were used for pairwise comparisons among groups (α=0.05). The one-way ANOVA test indicated that expression values of the growth factors, PDGF and bFGF, were significantly affected by irradiation of different wavelengths of lasers (p<0.001). However, expression value of the TGF-β was not affected by irradiation of different wavelengths of lasers (p>0.05). The highest PDGF expression was detected in neodymium-doped yttrium aluminum garnet (Nd:YAG) laser group (p<0.05), and there were no statistically significant differences among the other groups (p>0.05). The highest bFGF expression was detected in 980-nm diode and Nd:YAG laser groups (p<0.05), and there were no statistically significant differences among the other groups (p>0.05). These findings suggest that low-level Nd:YAG and 980-nm diode laser therapy accelerate the wound healing process by changing the expression of PDGF and bFGF genes responsible for the stimulation of the cell proliferation and fibroblast growth.
Effect of Low-Level Laser Therapy on Wounds in Intra-Oral Surgery
Oral Health Case Reports, 2016
During the last two decades, much has been written in both the scientific literature and the popular press about lasers and their use in dentistry. Both soft-and hard-tissue applications have been discussed, including frenectomy, gingival contouring, caries removal and bleaching. The aim of this study is evaluate and compare the effects of laser therapy on the surgical wound repair process and painful symptoms in patients undergoing bilateral teeth extractions at the Department of Dentistry, State University of Paraiba. Three sessions of laser therapy (GaAlAs) were performed for each patient. The first session was immediately after the surgical procedure, the second occurred 48-72 h after the first session, and the third and final at 7 days after surgery, coinciding with the removal of the sutures. Of the patients examined, 65.5% at the first assessment (after the second laser therapy session) and 75% at the second assessment (after the third session) exhibited a better degree of repair on the side treated with laser (STL) compared to the side not treated with laser (SNTL). There was no difference in edema between the STL and SNTL. Regarding pink tissue colour, there was an increase in its frequency, from 25%-62.5% of cases, on the STL between the first and second assessments. Bleeding also showed an increased frequency on the STL and decreased on the SNTL in the second assessment. Regarding painful symptoms, the results revealed that, on the STL, they decreased, from 62.5%-0% between the first and second assessments, and on the SNTL, from 87.5%-12.5%. Hence, low-power laser use appeared to be effective for the repair of intraoral surgical wounds, accelerating the degree of repair, improving tissue colour and reducing pain symptoms.