A histological study of the effect of the low level laser therapy on wound healing (original) (raw)
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Low level diode laser accelerates wound healing
Lasers in Medical Science, 2012
The effect of wound illumination time by pulsed diode laser on the wound healing process was studied in this paper. For this purpose, the original electronic drive circuit of a 650-nm wavelength CW diode laser was reconstructed to give pulsed output laser of 50 % duty cycle and 1 MHz pulse repetition frequency. Twenty male mice, 3 months old were used to follow up the laser photobiostimulation effect on the wound healing progress. They were subdivided into two groups and then the wounds were made on the bilateral back sides of each mouse. Two sessions of pulsed laser therapy were carried along 15 days. Each mice group wounds were illuminated by this pulsed laser for 12 or 18 min per session during these 12 days. The results of this study were compared with the results of our previous wound healing therapy study by using the same type of laser. The mice wounds in that study received only 5 min of illumination time therapy in the first and second days of healing process. In this study, we found that the wounds, which were illuminated for 12 min/session healed in about 3 days earlier than those which were illuminated for 18 min/session. Both of them were healed earlier in about 10-11 days than the control group did.
Effect of low power laser on incisional wound healing
Iranian endodontic journal, 2006
The effectiveness of low power lasers for incisional wound healing, because of conflicting results of previous research studies, is uncertain. Therefore, this study was carried out to evaluate low power laser effects on incisional wound healing. Incisional wound was produced on thirty-six mature male guinea pigs under general and local anesthesia. In half of the cases, HeNe laser radiations were used for five minutes and the rest were left untreated Animals were divided into six groups of six animals each that were killed after 3, 5 and 14 days. After histopathology processing and H&E staining, specimens were examined for acute and chronic inflammations, epithelial cell migration, epithelial seal and barrier formation, fibroblast migration, fibrosis, clot formation and granulation tissue formation. Mann-Whitney U and the Wilcoxon tests were used for statistical analysis. Statistically significant differences were found between fibroblast migration, acute and chronic inflammation of ...
Journal of Lasers in Medical Sciences
Introduction: Wound healing is a dynamic, interactive process to achieve the restoration of skin integrity and proper function after damage. Applying a low-level laser (LLL) and light emitting diodes (henceforth LEDs) is introduced in previous studies to accelerate the process of wound healing. The aim of this study is to compare the effect of the LLL and LEDs on wound healing in rabbits. Methods: Full thickness same size square excision wounds were created on the dorsum of the rabbits. Twenty rabbits were randomly divided into four groups, according to the treatment received. Group 1: the AlGalInP (aluminium gallium indium phosphide) laser (4 J/cm²); group 2: the red LED (30 J/cm²); group 3: the blue LED (60 J/cm²) and group 4, as the control group, was not irradiated. After 30 days, the wounds were evaluated both morphologically and histopathologically. Statistical significance was defined as a P value of less than 0.05. Results: All interfering methods including the LLL and LEDs ...
The Effect of Low Level Laser Therapy on Surgical Wound Healing
2010
Surgical wounds are superficial or deep wounds on skin or mucous membranes due to a surgical intervention using a scalpel to cut through skin or mucosa and the underlying tissue. The wound treatment costs in terms of finance and time are relatively high; the complete wound healing lasts 3 weeks. To reduce this period of healing of surgical wound and avoid complications, therapeutic methods and drug are used in postoperative period. One of these methods is Low Level Laser Therapy. This method has been used for treatment of wounds for over two decades in many medical facilities of the world. However, despite such wide clinical usage, there is still controversy regarding the efficacy of Low Level Laser Therapy in the treatment of wounds. Many laser systems, different laser parameters and irradiation conditions, and a great variety of treatment protocols lead to these conclusions. The goal of this paper is to present our evaluation of Low Level Laser Therapy role in surgical aseptic wou...
Laboratory methods for evaluating the effect of low level laser therapy (LLLT) in wound healing
African Journal of Biomedical Research, 2006
The basic tenet of laser therapy is that laser radiation has a wavelength dependent capability to alter cellular behaviour in the absence of significant heating. Low intensity radiation can inhibit as well as stimulate cellular activity. Laser therapy typically involves the delivery of 1-4J/cm 2 to treatments sites with lasers having output powers between 10mW and 90mW. There are two major areas of laser therapy research: the laboratory and the clinic. The laboratory presents the least ambiguous results. Here, although unsupported results do appear, the vast majority of published work finds clear evidence that laser irradiation alters cellular processes in a nonthermal, wavelength-dependent manner. Low energy laser irradiation alters t he cellular function by effecting protein synthesis, cell growth and differentiation, cell motility, membrane potential and binding affinities, neurotransmitter release, ATP synthesis and prostaglandin synthesis. Laboratory findings provide scientific rati onale of laser therapy and the effect of laser therapy on cellular processes. This review outlines some of the current methods employed in the laboratory to measure the effect of low level laser therapy (LLLT) on cellular and molecular processes in the cell. This review briefly explains the different structural, cellular and molecular parameters and highlights some of the basic principles and protocols including specialized equipment requirements.
635NM Diode Laser Biostimulation on Cutaneous Wounds
Biostimulation is still a controversial subject in wound healing studies. The effect of laser depends of not only laser parameters applied but also the physiological state of the target tissue. The aim of this project is to investigate the biostimulation effects of 635nm laser irradiation on the healing processes of cutaneous wounds by means of morphological and histological examinations. 3-4 months old male Wistar Albino rats weighing 330 to 350 gr were used throughout this study. Low-level laser therapy was applied through local irradiation of red light on open skin excision wounds of 5mm in diameter prepared via punch biopsy. Each animal had three identical wounds on their right dorsal part, at which two of them were irradiated with continuous diode laser of 635nm in wavelength, 30mW of power output and two different energy densities of 1 J/cm 2 and 3 J/cm 2. The third wound was kept as control group and had no irradiation. In order to find out the biostimulation consequences during each step of wound healing, which are inflammation, proliferation and remodeling, wound tissues removed at days 3, 7, 10 and 14 following the laser irradiation are morphologically examined and than prepared for histological examination. Fragments of skin including the margin and neighboring healthy tissue were embedded in paraffin and 6 to 9 um thick sections cut are stained with hematoxylin and eosin. Histological examinations show that 635nm laser irradiation accelerated the healing process of cutaneous wounds while considering the changes of tissue morphology, inflammatory reaction, proliferation of newly formed fibroblasts and formation and deposition of collagen fibers. The data obtained gives rise to examine the effects of two distinct power densities of low-level laser irradiation and compare both with the non-treatment groups at different stages of healing process.
Laser Therapy for Wound Healing: A Review of Current Techniques and Mechanisms of Action
Biosciences Biotechnology Research Asia
Along with conventional medications, different techniques have been used for wound healing such as ultrasound, electric field, magnetic field, pressure relieving beds, cushions, etc. These methods are usually utilized for prevention and healing of pressure wounds. One of these methods with great potential is LASER (Light Amplification by Stimulated Emission of Radiation) therapy. Different parameters can affect the efficiency of laser therapy. Several studies have attempted to develop this technique in different medical applications. This paper aims to provide a review of the LASER techniques for wound healing, sketch their background, determine the biological effects that support the use of LASER sources in the treatment of wounds and as well as the optimal light parameters such as wavelength and dose for wound healing Keywords : Laser, Wound Healing, Mechanism of Action
Assessment to the Effects of Low Power Diode Laser on Wound Healing in Diabetic Rats
Objective: Evaluation of the effect of low level laser therapy (LLLT) using diode laser 808 nm on wound healing in diabetic rats as experimental animal model. Back ground data: Delayed wound healing is reported by several medical care units as changing cases. One of the causes for chronic wounds and delayed wound healing is diabetes which sometime associated with suppuration, gangrene and may be ended by amputation. This is encountered in different medical specialities. Methods: 40 male albino rats, each weighed 200-220 gm. Diabetes was chemically induced using streptozotocin, 40 mg/kg, dissolved in citrate buffer solution (pH 4.3) and administered as tail vein injection in all experimental rats groups expect control group . Seven days after streptozotocin injection, blood glucose levels were measured by using a glucometer and test strips. Results: Gross examination showed faster wound closure in the laser exposed groups with minimal scar tissue formation in comparison with non-lase...
Biophotonic Effects of Low-Level Laser Therapy at Different Wavelengths for Potential Wound Healing
Photonics
Our objective was to assess the effect of low-level laser therapy (LLLT) administered using a diode laser on the growth processes of human fibroblast cells involved in wound healing. Initially, studies were conducted using a diode laser at wavelengths of 633, 520, and 450 nm with an irradiance of 3 mW/cm2. The distance between the light source and culture plate was 3 cm. The mechanism(s) of action of the diode laser illumination on human fibroblast cells were studied by examining different wavelengths to determine the relevant light parameters for optimal treatment. In addition, the percentages of fibroblast-mediated procollagen and matrix metallopeptidase (MMP)-1, -2, and -9 production were compared. In the clinical study, the changes in basic fibroblast growth factor (bFGF), vascular endothelial growth factor (VEGF), and fibroblast collagen production were assessed in 60 patients with complicated wounds who received LLLT (633 nm). No statistically significant difference was observ...