Rapid Heterotrophic Ossification with Cryopreserved Poly (ethylene glycol-) Microencapsulated BMP2-Expressing MSCs (original) (raw)

Fibronectin within Sodium Alginate Microcapsules Improved Osteogenic Differentiation of BMMSCs in Dose Dependent Manner by Targeting SP7, OCN, CDK1, ZBTB16, and Twist1 Expression

Advanced Pharmaceutical Bulletin

Introduction: Insoluble fibronectin as an extracellular matrix (ECM) protein has the potential to promote proliferation, differentiation, and migration of mesenchymal stem cells (MSCs). However, there is limited information about the effects of fibronectin various concentrations on bone marrow-derived MSCs (BMMSCs) function and differentiation. Materials and Methods: In this experimental study, using a gel injection device, BMMSCs were encapsulated in sodium alginate microcapsules containing 1.25% alginate, 1% gelatin, and four different concentrations of fibronectin (0.01, 0.05, 0.1, and 0.2 µg/ml). MTT assay was used to examine the proliferation of BMMSCs in des. Also, BMMSCs apoptosis rates were calculated using Annexin-V/PI staining and FACS analysis within 48 hours of exposure. Alkaline phosphatase (ALP) test was conducted to assess BMMSCs osteogenic differentiation. Finally, mRNA expression levels of the SP7, osteocalcin (OCN), Twist Family BHLH Transcription Factor 1 (Twist1)...