Hepatoprotective effect of Ocimum gratissimum against carbon tetrachloride induced hepatic damage model in rats (original) (raw)
Related papers
Journal of Pharmaceutical Negative Results
Modern medicine does not have reliable liver protection drugs that prevent and treat liver injury caused by drugs. The leaves of sacred / holy basil (Ocimum sanctum) belong to the family of lamiaceae, which is traditionally used for its liver protection. We aimed to determine if Ocimum sanctum has hepatoprotective properties and, if so, whether or not they work synergistically with silymarin. Albino rats (150–200 g) were divided into five groups. Groups A and B are normal control groups and experimental control groups, respectively. Groups C, D and E received the alcoholic extract of Ocimum sanctum leaves (OSE) 200 mg / kg BW / day,, silymarin 100 mg/kg BW/day and OSE 100 mg/kg BW/day + silymarin 50 mg/kg BW/day p.o., respectively, for 10 days. Hepatotoxicity was induced in Groups B, C, D and E on the eighth day with paracetamol 2 g/kg BW/day. The effect of hepatoprotection was evaluated through a study of serum proteins, albumin-globulin ratios, alkaline phosphatase, transaminase, ...
Ocimum gratissimum as a Remedy to Chemical Induced Liver Injury
Journal of Complementary and Alternative Medical Research
Objective: Drug induced liver injury is one of the sequelae of adverse drug reactions. We studied the efficacy of aqueous extract of Ocimum gratissimum at ameliorating carbon tetrachloride induced liver injury. Methodology: The study utilized five groups with six animals each. Liver injury was induced in groups L I (Liver injury), LDE (Low dose extract), MDE (Medium dose extract) and HDE (High dose extract) while group CN served as control. Groups LDE, MDE and HDE respectively had 100 mg/kg, 200 mg/kg and 400 mg/kg of the O.gratissimum aqueous extract daily for twenty one days.Blood samples were thereafter collected for biochemical analyses, the animals sacrificed and harvested livers processed for histopathological evaluation. Results: The mean liver weight of LI was markedly elevated while its total protein level was significantly the least. Extract of O.gratissimum was able to counter the elevated levels of aspartate transaminase and amino transaminase occasioned by the induced l...
AIM: The plant Ocimum canum Sims (Family: Lamiaceae) is rich in antioxidants, particularly in flavonoids. It has been highly useful in treating various types of diseases and in lowering blood glucose, especially in managing diabetes mellitus. The aim of our work is to evaluate the effect of Ocimum canum on a model of hepatic ischemia-reperfusion in Rat. METHOD: Wistar albino rats were subjected to 45 min of hepatic ischemia, followed by a 60 min reperfusion period. Ocimum canum (OC) hydro-alcoholic leaf extract were administered in doses of 100, 200 and 400 mg/kg/day, orally for 15 days before I/R injury respectively and repeated before the reperfusion period. Liver samples were taken for histological examination or determination of hepatic malondialdehyde (MDA), super oxide dismutase (SOD), catalase and glutathione (GSH) activity. Serum aspartate aminotransferase (AST), alanine aminotransferase (ALT,) alkaline phosphatase (ALP) and total bilirubin levels were determined to assess l...
Pharmacognosy Research, 2011
skills and practices based on the theories, beliefs and experiences indigenous to different cultures that are used to maintain health as well as to prevent, diagnose, improve or treat physical and mental illnesses. Herbal treatments are the most popular form of traditional medicine. Herbal medicines include herbs, herbal materials, herbal preparations and finished herbal products that contain parts of plants or other plant materials as active ingredients. [2] However, no scientific data regarding the identity and effectiveness of these herbal products were available, except in the treatise of Ayurveda and Unani medicine. [3] The World Health Organization (WHO) has laid emphasis on promoting the use of traditional medicine for health care. [2] Hence, we see a focus on research on traditional and herbal medicine, especially in developing countries, with individual as well as collaborative efforts by national research organizations. [4] Background: There is a lack of reliable hepatoprotective drugs in modern medicine to prevent and treat drug-induced liver damage. Leaves of Sacred/Holy Basil, i.e. Green Tulsi (Ocimum sanctum), belonging to family Lamiaceae are used traditionally for their hepatoprotective effect. We wanted to evaluate the hepatoprotective activity of Ocimum sanctum and observe whether synergistic hepatoprotection exists with silymarin. Materials and Methods: Albino rats (150-200 g) were divided into five groups. Groups A and B were normal and experimental controls, respectively. Groups C, D and E received the alcoholic extract of Ocimum Sanctum leaves (OSE) 200 mg/kg BW/day, silymarin 100 mg/kg BW/day and OSE 100 mg/kg BW/day + silymarin 50 mg/kg BW/day p.o., respectively, for 10 days. Hepatotoxicity was induced in Groups B, C, D and E on the eighth day with paracetamol 2 g/kg BW/day. The hepatoprotective effect was evaluated by performing an assay of the serum proteins, albumin globulin ratio, alkaline phosphatase, transaminases and liver histopathology. The assay results were presented as mean and standard error of mean (SEM) for each group. The study group was compared with the control group by one-way ANOVA, followed by Bonferoni's test. A P-value of <0.01 was considered significant. Results: In groups C, D and E, liver enzymes and albumin globulin ratio were significantly (P < 0.01) closer to normal than in group B. Reduction in sinusoidal congestion, cloudy swelling and fatty changes and regenerative areas of the liver were observed on histopathological examination in groups C, D and E, whereas group B showed only hepatic necrosis. Conclusion: The Ocimum sanctum alcoholic leaf extract shows significant hepatoprotective activity and synergism with silymarin.
Effects of few Indian medicinal herbs on carbon tetrachloride induced hepatic injury in animals
2009
The Phyllantus nirruri (PN), Andrographis paniculata (AP) and Picrroriza kurroa (PK) have been widely used in number of hepatoprotective formulations based on their traditional claims. However, no published data showing the comparative study of these herbs is available. The aim of the present study was to evaluate the hepatoprotective effect of standardised herbal extracts of PN 142.5 mg/kg, p.o), AP (300 mg/kg, p.o) and PK (200 mg/kg, p.o) on carbon tetrachloride (CCI 4) induced acute and chronic hepatic damage in rats. In chronic hepatitis, CCI 4 (0.2 ml/kg, p.o) was administered twice weekly during eight weeks of extract treatment, whereas, in acute hepatitis, CCI 4 (0.5 ml/kg, p.o) was given on 10 th day of administration of extract. Liver injury was analyzed by estimating the biochemical marker enzymes levels and antioxidant activities. Histological studies were also carried out. Statistically significant reversal of the elevated serum levels and depleted tissue levels of biochemical marker enzymes were found as the biochemical indices for hepatoprotection. The histological scores and liver weight was significantly reduced as well as SOD and catalase activities were significantly increased in treated groups compared to CCI 4 control. Further, PK and PN were found to be most and least effective respectively in chronic CCI 4 induced liver damage, whereas, AP exhibited least protection than PN and PK in acute CCI 4 hepatic injury. These results show that the standardised extracts PN, AP and PK possess hepatoprotective potential and prevented hepatic damage induced by CCI 4 .
2012
This study was conduced to investigate the effect of some Egyptian herbs on impaired liver function of rats injected with Carbon Tetrachloride (CCl4). Seventy mature albino rats, weighting 150-160g per each, were used and divided into 12 equal groups, one was kept as a control-ve group, while the other groups were injected subcutaneous (s/c) by CCl4 in paraffin oil 50% v/v (2 ml/kg B.W.), twice a week for tow weeks. The tested herbs were given as a percent of 5% and10% from the Basel diet. Serum liver function (GOT, GPT, ALK) total protein, albumin, globulin, total bilirubin, indirect bilirubin, direct bilirubin, and histopathological changes of liver were examined. The results indicated that rats treated with CCl4 recorded significantly increasing in the activity levels of all biomarker liver enzymes (SGOT, SGPT and SALP) and decreasing in serum total protein, albumin, globulin and bilirubin with significant values when compared with the control group after the experiment period, 2...
ijpbs.net
The Poly herbal ethanolic extract (PHEE) was evaluated for its anti hepatotoxic potential against carbon tetrachloride (CCL 4 ) induced hepatic damage in male albino rats. Ethanolic extract from the leaves of Melia azadirachta, seeds of Piper longum and whole plant of Eclipta alba at a dose level of 50 mg/kg body weight was administered orally daily once for 14 days. The substantially reduced levels of enzymatic anti-oxidants such as superoxide dismutase(SOD), catalase(CAT), glutathione peroxidase (GPx), glutathione-stransferase(GST) and glutathione reductase (GR), due to carbon tetra chloride effect were restored to normal. Histopathological examination of liver sections of PHEE treated rats shows the restoration of normal tissues. Vitamin-E at a dose of 100 mg/kg taken as a standard reference also exhibited significant antioxidant activity against CCl 4 induced Hepatotoxicity. The results of this study strongly justified that PHEE has a potent hepatoprotective activity against carbon tetrachloride
Benha Veterinary Medical Journal, 2019
The aim of this study was to evaluate pulmonary function test, acute phase proteins, pro-inflammatory cytokines, electrolyte balance and electrocardiographic alterations in BRD affected calves compared to pen matched healthy control calves. A total of 30 calves (20 BRD affected calves and 10 apparently healthy calves) were used in this study. The BRD affected calves showed significant (P<0.05) reduction in blood pH, partial pressure of oxygen, oxygen saturation, calcium, phosphorus, magnesium, sodium and chloride, and significant (P<0.05) increase in partial pressure of carbon dioxide, total carbon dioxide and potassium. Acute phase proteins and Proinflammatory cytokines assessment revealed a significant (P<0.05) increase of serum amyloid A, haptoglobin, fibrinogen and interleukin-6 in diseased calves compared to healthy control calves. Electrocardiographic examination of BRD affected calves revealed a significant increase in heart rate with a significant (P<0.05) alteration in ECG wave trace parameters (P wave, QRS complex, T wave, PR interval, QT interval). In conclusion, measurement of pulmonary function test, APPs, cytokines and ECG could be used as valuable and early diagnostic tools for BRD diagnosis in feedlot cattle calves.
Beneficial Effects ofOcimum gratissimumAqueous Extract on Rats with CCl4-Induced Acute Liver Injury
Evidence-Based Complementary and Alternative Medicine, 2012
Ocimum gratissimum(OG) is known as a food spice and traditional herb, which has been recommended for the treatment of various diseases. To investigate the hepatoprotective effect of OG aqueous extract (OGAE), male Wistar rats challenged by carbon tetrachloride (CCl4) were used as the animal model of chronic hepatic injury. Significantly increased serum catalase and DPPH levels were detected in CCl4-administrated rats that were treated with OGAE or silymarin as compared to those rats that were treated with saline or CCl4. In contrast, significantly decreased stress proteins including HSP70 and iNOS were observed in livers of CCl4-administrated rats that were treated with OGAE or sylimarin as compared to those rats that were treated with saline or CCl4. Moreover, significant decreases of MMP-9/MMP-2 ratio, uPA, phosphorylated ERK (p-ERK) and NF-κB (p-P65) were detected in livers of CCl4-administrated rats that were treated with OGAE or sylimarin as compared to those rats that were tre...
Evaluation of the anti-hepatotoxic activity of methanoldichloromethane (MDM) extract of Oroxylum indicum (OI) whole plant on carbon tetrachloride (CCl 4 )-induced hepatotoxicity in rat liver explant cultures (in-vitro) and chronic liver damage in rats (in-vivo). In toxicant (CCl 4 ) treated explants culture set (in-vitro), the marker enzymes GOT, GPT and ALP level increased significantly (p<0.001) when compared to control set. Treatment of CCl 4 -intoxicated liver explant culture sets with MDM extract of OI whole plant at a concentration of 3.3 mg/ml attenuated the marker enzymes GOT, GPT and ALP levels in culture supernatants significantly (p<0.001) as compared to CCl 4 treated explant culture sets. A significant hepatocellular damage as evident from significant elevation in serum activities of GOT, GPT, ALP, bilirubin concentration (total and direct) with decrease in albumin and total protein were found in CCl 4 : liquid paraffin (1:1) treated animals (in-vivo) when compared to normal values. The MDM extract of OI whole plant (200 mg/kg/day, p.o.) exhibited a significant reduction (p<0.001) in CCl 4 : liquid paraffin (1:1) induced increased serum levels of GOT, GPT, ALP and bilirubin concentration whereas the albumin and total protein content increased thus reversing hepatotoxicity causing significant liver recovery. Mild swelling of hepatic cells with minimal hepato-cellular injury is shown in T.S of liver tissue from OI extract treated group in contrasts to toxic group. The study suggested the hepatoprotective activity of MDM extract of O. indicum whole plant in-vitro and in-vivo against CCl 4 -induced hepatotoxicity.