Determination of Bacteria Resistance Threshold to Lead, Zinc and Cadmium in Three Industrial Wastewater Samples (original) (raw)
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Lead and cadmium resistant bacteria isolated from industrial effluent
Bacteria play a major role in the biogeochemical cycling of toxic heavy metals. Heavy metal resistant bacteria can be used as bioremediation agents. The aim of this present investigation was to determine the resistance property of bacteria isolated from industrial waste dumped soil samples, to heavy metals (Pb2+ and Cd2+) and some antibiotics. Of 29 isolates, only two bacterial strains were found to be highly resistant to lead and cadmium. Further, these two bacterial strains were checked for resistance against heavy metals by culturing them in basal medium in which varying concentrations of heavy metals were incorporated. HML8 and HMC1 isolates showed resistance against Pb2+ and Cd2+ at all concentrations tested. The morphological and biochemical tests confirmed that the isolates belonged to Bacillus sp. Both the isolates showed similar pattern of growth at different temperature ranging from 25⁰C to 42⁰C. HMC1 showed better growth in the presence of high NaCl concentration but the same was unable to grow at high pH. On the other hand HML8 showed good growth in the basal medium at high pH. High salt concentration affected the growth of HML8 in the basal medium. Both the isolates also exhibited high tolerance to antibiotics such as Amoxicillin and Penicillin G. The results of the present study clearly suggest that both the strains can be studied and explored further for biotechnological applications especially for lead and cadmium detoxification.
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Thirty heavy metal resistant bacteria were isolated from sewage of industrial effluents, garages and petrol pumps of Barak Valley region of Assam, India, against cadmium and lead. Samples were streaked on selective media; the predominant and distinct colonies were identified as Pseudomonas sp., Klebsellia sp., Staphylococcus sp., Proteus sp. and Bacillus sp. on the basis of their biochemical and morphological characters. Minimum inhibitory concentration (MIC) and antibiotic resistance pattern of the potent isolates was also studied. Among all, six isolates exhibited high resistance to heavy metals. Bacillus sp. was found to have high resistance pattern against Cadmium (1800 µg/ml) and Lead (1200 µg/ml). It was observed that the isolates having high MIC values for a set of metals exhibited high resistance pattern towards a group of antibiotics.
آب و فاضلاب, 2017
Municipal and industrial effluents continually release into the environment heavy metals of a variety of physical and chemical forms and at various concentrations. Biological treatment processes have attracted a growing attention for the removal of heavy metals from these effluents. For the purposes of the present study, bacteria that are relatively resistant to heavy metals, such as cadmium and lead, were isolated from municipal waste and purified. They were then subjected to biochemical tests for identification and their minimum inhibitory concentrations were determined. Bacterial minimum inhibitory concentrations were initially measured in flasks containing 25, 50, 75, 100, 150, 300, 500, and 700 ppm of lead and cadmium before superior bacteria at populations of 108 CFU/ml were evaluated in terms of their ability to remove lead and cadmium at concentrations of 50, 100, 150, and 300 ppm from enriched municipal wastewater. Base on the results, Bacillus laterosporous and Yersinia ps...
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Cadmium and lead tolerant bacteria isolated from industrial waste water
Dhaka University Journal of Biological Sciences, 2017
An in vitro study was conducted to isolate, identify and characterize heavy metal resistant bacteria from industrial waste water and to determine their tolerance capacity to cadmium and lead. Different morphological, physiological and biochemical tests were carried out to identify the bacterial isolates in the waste water. A total of eight bacterial isolates viz., Staphyllococcus intermedius, Pseudomonas aeruginosa, Bacillus cereus, Bacillus subtilis, Escherichia coli, Acinetobacter baumanii, Pseudomonas flavescens and Acinetobacter lwofii were identified from metal polluted tannery and steel industrial areas. Bacterial response to cadmium tolerance was determined by treating them with CdCl2 solution at a rate of 1, 2, 2.5 and 5 µg/ml. In case of lead tolerant bacteria, PbCl2 solution was applied at a rate of 0.05, 0.125, 0.2 and 0.5 µg/ml. Pseudomonas aeruginosa isolated from waste water of steel industries and Bacillus cereus from tannery area was found to be the most tolerant species to the different doses of cadmium and lead. The study indicated that Pseudomonas aeruginosa and Bacillus cereus could be good candidates for the treatment and elimination of heavy metals from industrial waste water. The present study may be helpful to the bioremediation of heavy metals in the contaminated environment.
Heavy metal pollutants are commonly found in soils, sediments and waste water where they remain persistent, and become toxic to many species above certain threshold concentrations. Bacteria resistant to heavy metals can be used for detoxification and prevent further deterioration of contaminated sites. The incidence of bacteria resistant to heavy metals in soils collected from six contaminated sites in Lagos and Ota, Nigeria was assessed in this study. Heavy metal resistant bacteria were isolated from the soil samples following enrichment by growth on media amended with high concentrations of lead, cadmium and chromium, and identified based on cultural, morphological and biochemical characteristics. A total of 11 heavy metal resistant bacteria isolated from the soil samples were tentatively identified as Aeromonas sp (3), Arthrobacter sp. (1), Corynebacterium sp (4), Pseudomonas sp (1) and Streptococcus sp (2). The maximum tolerated concentrations of metals (in basal medium suppleme...
Journal of Applied Research in Water and Wastewater, 2023
Industrial activities present a significant threat to the environment and natural ecosystems like water and soil due to the release of toxic metals. This article primarily concentrates on the identification and isolation of bacteria, with the goal of effectively eliminating pollutants from industrial wastewater. In order to achieve this goal, the study was conducted to assess the ability of bacterial strains to tolerate copper (Cu) and zinc (Zn), as well as their antibiotic resistance and ability to tolerate elevated metal concentrations. The resistance of the isolates to various metals and antibiotics were assessed using the minimum inhibitory concentration (MIC) values and disc diffusion (DD) method, respectively. The technique of colony PCR was employed to determine the identity of the bacteria that were separated. Resistance to multiple antibiotics was assessed, including Penicillin, Sulfamethoxazole, Tetracycline, Erythromycin, Amoxicillin, Cefoxitin, Streptomycin, Chloramphenicol, Vancomycin, Gentamycin, Cephalothin, Rifampicin, and Novobiocin. In the current investigation, a total of 5 bacteria with a positive gram stain and 7 bacteria with a negative gram stain were identified. The study found that the effluent from the wastewater treatment plant in Razi industrial town showed resilience to copper ions, especially at a concentration of 7mM. The effluent wastewater from the refinery unit exhibited the greatest level of tolerance towards zinc, with a concentration as high as 6mM. The rise in copper and zinc levels in industrial wastewater treatment plants causes microorganisms to develop resistance to these heavy metals. The study of Gram-positive resistant bacteria conducted in this research focused on the examination of their susceptibility to zinc and copper. Notably, Staphylococcus hominis displayed resistance to a majority of the antibiotics evaluated. However, Kocuria rosea demonstrated sensitivity to all antibiotics. Agrobacterium fabrum exhibited susceptibility to all antibiotics as opposed to other Gram-negative bacteria resistant to zinc and copper. The findings of this study indicated that some strains displayed a degree of resistance to both antibiotics and heavy metals. The presence of heavy metals in bacteria isolated from a wastewater treatment plant exhibited the capability to restrict antibiotic resistance.
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Heavy metals released from various industrial effluents have damaging effects on ecosystem and may become a health hazard to man and animals. Some heavy metals at low concentrations are essential micronutrients for various life forms, but in higher concentrations, they tend to cause metabolic disorders and growth inhibition. Microorganisms have evolved several mechanisms to tolerate the presence of heavy metals by efflux, complexation or reduction of metal ions to non toxic forms. Microorganisms isolated from industrial effluent discharges were found to tolerate high levels of Cadmium (3000 ppm), Lead (600 ppm), Arsenic (1500 ppm) and Mercury (500 ppm). These isolates were seen to have high level of tolerance to various xenobiotic compounds like pesticides and showed multi-drug resistance. The residual heavy metals (Cadmium, Lead, Arsenic and Mercury) after bioaccumulation were analyzed using ICP-AES technique and the isolates showed 70-80% bioaccumulation of heavy metals. The metal...
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The incidence of chemical stressors in industrial waste effluents has culminated in the re-engineering the genetic and metabolic characteristic of resident microbiota. Microbial adaptability enables them to tolerate these stressors however, propelling the phenomena of acquisition of heavy metal resistance which may also incite resistance to antibiotics. Waste water from industrial establishments may travel from site into surrounding communities via canals and waterways thus, disseminating these stressors as well as resistance in the environment. This study seeks to investigate the physicochemical and heavy metal composition of industrial effluent and its tolerance in resilient bacteria from the study area. Physiochemical analyses revealed pH level which ranged between (5.8-10.87), BOD (6.612-16.01 mg/l), TDS (937.226-2173.49 mg/l), Sulphates (658.72- 1342.28 mg/l), Nitrates (11.46-70.16 mg/l), Phosphate (3.03-8.43 mg/l) exceeded the NESRA limits; Cu (0.024-4.521 mg/l) Cd (0.002-6.41...
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Water is the most basic element for the life on earth. Reliable access to cleanly and affordable water is considered main challenge for the total world. Along with waste water progress, environmental pollutants like toxic heavy metals are widely spreading throughout the world. The presence of heavy metal in aqueous environment poses a dangerous environmental risk.The utilization of heavy metal resistance bacteria might avail a cost-efficient tool for bioremediations of contaminated water bodies. During our screening program for heavy metal resistant bacteria isolated from agriculture wastewater sample collected from mainsheet El Awqaf near kafr el-Dewar), EL-Bahaira Egypt was able to grow on (LB) medium supplemented with Cadmium (50 to 500 mg/l),six isolates were chosen (4(DB-3), 5(DB-3), 6CB-3), 7(CB-7), J (CB-5), and M (CB-3) based on the metal tolerance concentration (MIC) values these isolates were gram negative rod shaped bacteria.