Effect of Types Isolated Lactic Acid Bacteria on Hematocrit and Differential Leukocytes Fingerling Common Carp (Cyprinus carpio L.) Infected with Aeromonas hydrophila bacteria (original) (raw)
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Jurnal Iktiologi Indonesia, 2021
This study aims to find out the right lactic acid bacteria (LAB) isolates to improve the common carp body's immune system against the attack of the Aeromonas hydrophila bacterium. Lactic Acid Bacteria (LAB) isolates were obtained from the organs of the common carp intestine. The study used a Completely Randomized Design consisting of four treatments and three replications. The treatment used was immersion of test fish for 24 hours in several isolates of LAB with a density of 108 cfu ml-1, namely A: without LAB isolate (control), B: isolate CcB7, C: isolate CcB8, D: isolate CcB15. Immersion is done three times with a frequency of seven days. Parameters observed included clinical symptoms, survival rate, red and white blood cell counts. The results showed the immersion of LAB isolate CcB15, namely Lactobacillus gasseri was most effective for increasing the body's resistance to Common Carp, which was demonstrated by mild clinical symptoms and faster recovery, high fish survival...
The Israeli Journal of Aquaculture Bamidgeh, 2007
The effects of two diets, SGP 493 (Aller Aqua, Denmark; SGP) and chironomid larvae (Katrinex, Poland; CHI), on juvenile carp survival and hematological values after subcutaneous infection with Aeromonas veronii bt. sobria (strain K144) were studied. All infected fish developed skin ulcers and both groups developed anemia, i.e., a severe reduction in hemoglobin content without a decrease in red blood cell count and a minor decrease in hematocrit. There were higher levels of hematocrit, hemoglobin, and mean cell hemoglobin in the CHI group at the beginning of the infection, together with a slightly higher erythropoietic potential. At the end of the experiment, partial recovery of hemoglobin levels took place. The SGP diet induced considerable leukocytosis in healthy fish, which did not enhance their resistance to the infection. Leukopenia, lymphopenia, and reduced phagocyte activity took place in all infected fish. Survival 15 days after injection was 50% in SGP and 63% in CHI, suggesting that fish fed natural food were slightly less susceptible to Aeromonas-induced anemia even though feeding natural food or SGP did not significantly affect the performance of infected fish.
Advances in Bioscience …, 2010
The effects of injectable killed Aeromonas hydrophila on lymphocyte populations and on serum proteins of juvenile common carp, Cyprinus carpio, were examined. The fishes were injected either intraperitoneally or intramuscularly with killed A. hydrophila bacterin isolated from a diseased fish. 15 days after injection the lymphocyte count was significantly higher in the kidney of intraperitoneally bacteria injected fishes than in the normal saline injected fishes (p < 0.05), but in the blood smears the lymphocyte count difference was not significant (p > 0.05). In the intramuscular bacteria injected fishes in comparison with the normal saline group, the lymphocyte count was significantly higher only in the blood (p < 0.05) but in the kidney the difference was not significant (p > 0.05). In comparison between the serum protein parameters in different groups, in intraperitoneal injection of killed-bacteria, total albumin as well as the ratio of albumin/globulin was greater than the control group (p < 0.05). The other fractions had not showed any significant difference (p > 0.05). In intramuscular injection, the normal saline-injected group, amount of α-globulin was significantly higher than bacteria-injected group (p < 0.05). For the other fractions there was no significant difference between two groups (p > 0.05).
Aquaculture, 2004
Selection of fish disease resistance may be performed directly via survival and challenge data or indirectly via the identification of the underlying resistance markers responsible for the differential survival. In a preliminary study, the potential correlation of a variety of specific and nonspecific immune parameters with aeromoniasis resistance was investigated in 13 different fullsib families of rohu (Labeo rohita), one of the important Indian major carp species known to exhibit differential susceptibilities to aeromoniasis in laboratory conditions. The experimental infection of fish through the intraperitoneal route to Aeromonas hydrophila produced mortality ranging from 0 to 100%. The specific immune response as measured through haemagglutination (HA) titre against sheep red blood cells (SRBC) and nonspecific immunity levels (lysozyme activity and natural haemolysin titre) were not found to be significantly different between resistant and susceptible groups. Serum bactericidal activity was found to be higher in groups of rohu resistant to A. hydrophila infection. Nevertheless, significant (P < 0.05) positive correlation between serum bactericidal activity and aeromoniasis survivality was observed. Based on the observations, bactericidal activity appears to play a role in resistance against A. hydrophila infection.
The impacts of invasive species are second only to habitat destruction as a cause of global biodiversity loss. It imposes a great threat to economic development by reducing native fisheries production. The physiological effects of invasive bacterial pathogen Aeromonas salmonicida on some haematological, biochemical and non-specific immune response parameters were investigated on Indian Major carp, Labeo rohita (Hamilton, 1822) in this present study. Fishes were inoculated (i.p.) with 2 × 10 7 cfu/ml of A. salmonicida to generate asymptomatic carrier state. Significant (P < 0.05) alterations were observed in haematological, biochemical parameters viz. white and red blood cell counts, haemoglobin content, haematocrit, leucocrit, MCV, MCH, MCHC, dWBC % (neutrophil, eosinophil, lymphocytes, monocytes), total protein, glucose, calcium, bilirubin, alkaline and acid phosphatase activity in serum of treated fishes in comparison to the controls after 7 days exposure. Considerable differences (P < 0.05) were also observed in the bactericidal activity level of the serum collected at 7 days post infection indicating adequate functioning of the non-specific fish immune system after experimental bacterial challenge. Lastly re-isolation and detection of A. salmonicida was carried out by standard biochemical culture methods. The findings indicated that these parameters could be analyzed as potential exposure biomarker for clinical monitoring against exotic bacterial pathogen both in aquacultural conditions and natural habitats.
Veterinary Immunology and Immunopathology, 2007
The immune response to mixed whole cell antigens of Aeromonas hydrophila, Edwardsiella tarda and Pseudomonas fluorescens, the common Gram negative bacterial pathogens associated with diseases of Indian major carps were evaluated for their efficacy in triggering antibody responses in rohu, Labeo rohita (Ham.). The rohu yearlings were either immunized with antigens from single bacterial strain, A. hydrophila, E. tarda and P. fluorescens or a combination of all three. An antibody response was detected at 1st week post immunization that rose significantly (p<0.05) at 4th week post immunization in all the immunized groups. The antibody level started declining after 8th week but persisted up to 10th week post immunization in all the immunized groups. Similarly, no significant difference (p>0.05) in the antibody level was found between groups immunized with single and mixed bacterial antigens. Moreover, the use of mixed bacterial antigens did not jeopardize the specific immune response to the vaccine components. Upon challenge with single pathogen, a high relative percent survival was recorded in the group immunized with mixed bacterial antigens and was comparable to those fish immunized with the single bacteria.
2019
This study aims to determine the best density of Bacillus sp. which is given through feed to increase the body resistance of carp that is infected by pathogenic bacteria, Aeromonas hydrophila. The research was conducted at the Aquaculture Laboratory and Laboratory of Microbiology and Biotechnology at FPIK UNPAD. This study used an experimental method with a completely randomized design consisting of 4 treatments and 3 replications, namely treatment A (control), treatment B (Bacillus sp. 10 cfu/mL feed), treatment C (Bacillus sp. 10 cfu/mL feed) and treatment D (Bacillus sp. 10 cfu/mL feed) for two weeks later in Aeromonas hydrophila infection with an intramuscular density of 10 cfu/mL for seven days. Observation parameters were clinical symptoms, fish response to feed, fish response to shock and survival. The results of this study showed that treatment C (Bacillus sp. 10 cfu/mL feed) resulted in 89% survival and reduced clinical symptoms.
Journal of Aquaculture Engineering and Fisheries Research, 2015
The main clinical and pathomorphological changes in Aeromonas hydrophila infection in carp provoked by different field strains of this bacterium were studied. The strongest histopathological damages were seen in the functional epithelium of liver and kidneys, followed by intestine and hearth damages in addition to various haemorrhages in interstitial tissues of visceral organs and some skin haemorrhages on the ventral surface of the body and the anal region. As a whole, pathological damages consisted of degenerative changes as cloudy swelling, granular and/or hyaline droplet degeneration or vacuolation in functional epithelium in the respective internal organs in addition to some target vascular disturbances, which appeared to be good biomarkers for field assessment of that disease. Pathological damages in internal organs and haemorrhages were stronger in experimental fishes exposed to higher levels of the respective pathogens, especially those isolated from dead anaconda or the referent strain, and less pronounced damages were seen in fishes infected with of Aeromonas hydrophila isolated from a silver carp with marked signs of septicaemia.