FREE RADICAL SCAVENGING ACTIVITY, PHENOLIC CONTENTS AND PHYTOCHEMICAL ANALYSIS OF SEEDS OF Trigonella foenum graecum (original) (raw)
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Annals of phytomedicine, 2023
The leguminous family of plants includes fenugreek (Trigonella foenum-graecum L.), one of the oldest and most promising therapeutic herbs. Fenugreek is a member of the Fabaceae family. The goal of the current study was to evaluate the proximate composition, mineral analysis, phytochemical analysis and antioxidant potency of fenugreek seeds belonging to variety Hisar Mukta (HM) 425. The moisture content (%), crude protein (%), crude fat (%), ash (%), crude fiber (%), total carbohydrates (%) and mineral content (ppm) in fenugreek seeds were evaluated. The acetone extract of seeds was examined for a number of phytochemicals, including total phenolics (1.35 mg GAE/g), total flavonoids (0.12 mg CE/g), total sugars (17.34 mg/g), non-reducing sugars (17.18 mg/g) and reducing sugars (0.16 mg/g). The antioxidant activity was evaluated using the DPPH free radical scavenging activity and the phosphomolybdenum assay. These findings support the notion that fenugreek seeds are an abundant source of phytochemicals and minerals with pharmacological and health benefits.
Agricultural Science Digest – A Research Journal, 2023
Background: Plants contain phytochemicals that are crucial to their growth and development. The potential health benefits of dietary phytochemicals including flavonoids, isoflavones and other polyphenols. Polyphenolic substances, the largest class of phytochemicals, act as strong antioxidants due to abundance of hydroxyl groups. Trigonella foenum-graceum also known as Fenugreek is rich source of phytochemicals, therefore possess extraordinary healing and medicinal properties. Methods: The present study is designed to evaluate chief phytochemical constituents and antioxidant capacity of acetone extract of dried Trigonella foenum-graecum leaves (Variety HM 444). The phytochemicals like total phenolic content by Folin-Ciocalteu method, total flavonoids by AlCl 3 colorimetric assay, total sugars by method of Dubois and reducing sugars by method of Nelson as modified by Somogyi are determined in the acetone extract of leaves. The antioxidant capacity is determined by using 2, 2-diphenyl-1picrylhydrazyl (DPPH) free radical scavenging assay and phosphomolybdenum assay. Result: Various phytochemicals such as total phenolic content (3.17 mg GAE/g), total flavonoids (1.34 mg CE/g), total sugars (32.35 mg/g), Reducing sugars (0.53 mg/g) and Non-reducing sugars (31.82 mg/g) are investigated in acetone extract of leaves. The antioxidant activity of leaves extract is evaluated using the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay and reported an IC 50 value of 737.50 μg/mL. The total antioxidant capacity using phosphomolybdenum assay is 28.21 mg AAE/g.
2017
Aim: Fenugreek seeds are popular for their diverse therapeutic benefits. Processing intervention to improve nutritional properties of such agricultural seeds is an age old practice. In the current study, sprouted fenugreek seeds have been analyzed for their potential use as a home based approach for the management of oxidative stress related disorders. Methodology: Germinated seeds were dried at 60°C, powdered and stored at 4°C. The samples extract (10 mg/mL) in distilled water were then analyzed for Total Phenolic Content (TPC) and antioxidant activity by DPPH and FRAP. The results obtained for seven days were compared for significant differences using one-way ANOVA. Results: The process of sprouting showed significant changes in the antioxidant properties of fenugreek seeds with the maximum activity observed on 7th day of sprouting. An increase ranging from 40% to 45% in TPC and antioxidant activity was observed on the seventh day of germination. A correlation analysis among the T...
The main objective of this study was to evaluate antioxidant and anticancer potential of the extract and fractions from Trigonella Foenum graecum (fenugreek) leaves. The methanolic extract of the fenugreek leaves was sequentially fractioned in petroleum ether, chloroform, ethyl acetate, butanol and aqueous fractions. Qualitative screening of these fractions suggested presence of alkaloids, tannins, flavonoides, terpenoides, saponins, steroids and glycosides. The total phenolic content (38.48 mg gallic acid equivalent/g dry weight) and antioxidant activity (log IC 50 1.57±14.3 µg/ml) was found to be highest in butanol fraction of fenugreek leaves. Anti-proliferative assay revealed potent inhibitory effect of chloroform fraction (log IC 50 2.93±5.5 µg/ml) on breast carcinoma cells (MCF-7). The gas chromatography mass spectrometry (GC-MS) analysis of the chloroform fraction showed 20 peaks representing 81.72% of the total fraction. Di-(2-ethylhexyl) phthalate was identified as a major compound in chloroform fraction, suggesting its role in killing cancer cells. It is evident from these results that T. Foenum graecum contains bioactive compounds having anticancer and antioxidant potential and is recommended as a plant of phytopharmaceutical importance.
Antioxidant, phytotoxic and cytotoxic activity of methanolic extract of Trigonella foenum-graecum
Journal of Coastal Life Medicine, 2016
The journal implements double-blind peer review practiced by specially invited international editorial board members. Objective: To analyze the methanol extract of Trigonella foenum-graecum (T. foenumgraecum) for antioxidant, phytotoxic and cytotoxic activity. Methods: The powder of T. foenum-graecum was extracted in diluted methanol with the help of random shaking method. All extracts of the plant were measured for cytotoxic activity (beside brine shrimp and antioxidant activity vs. 1, 1-diphenyl-2-picrylhydrazyl free radical). Results: Various concentrations of methanolic extract of T. foenum-graecum were observed as 36.16% to 54.12% with rising concentrations of 50 to 1 000 μg/mL. Significantly phytotoxic activity (100 and 1 000 μg/mL) reduced the growth of roots (radicals) and shoots (hypocotyls) of rice when compared to control after 3 and 7 days' treatment. At a concentration of 10 μg/ mL, the survival rate of cytotoxic activity of brine shrimp was maximum and at a concentration of 250 μg/mL, the death rate of brine shrimp was maximum. Conclusions: T. foenum-graecum has potential activity against free radical mediated sickness and thus it is possible to treat cancer.
Food Chemistry, 2007
An extract of fenugreek (Trigonella foenum graecum) seeds was isolated and evaluated for antioxidant activity using various in vitro assay systems. The seed extract exhibited scavenging of hydroxyl radicals (Å OH) and inhibition of hydrogen peroxide-induced lipid peroxidation in rat liver mitochondria. The Å OH scavenging activity of the extract was evaluated by pulse radiolysis and the deoxyribose system. The antimutagenic activity of the extract was recorded by following the inhibition of c-radiation induced strand break formation in plasmid pBR322 DNA. The extract at high concentrations acted as a scavenger of 2,2 0-diphenyl-1-picryl hydrazyl hydrate (DPPH) and 2,2 0-azinobis 3-ethylbenzothiazoline-6-sulfonate (ABTS ÅÀ) radicals. The total phenolic content in the extract was determined spectrophotometrically according to the Folin-Ciocalteau procedure and expressed as mg or mM gallic acid equivalents. The results indicate that the extract of fenugreek seeds contains antioxidants and protects cellular structures from oxidative damage. These findings provide evidence for the in vivo beneficial effects of the seeds reported in the literature.
Annals of Phytomedicine: An International Journal, 2021
Fenugreek (Trigonella foenum-graecum L.), popularly known as methi, is a Leguminosae plant with many pharmacological and pharmaceutical benefits. Traditionally, it is used in Ayurveda, Chinese and Arabic systems of medicine. Fenugreek leaves, seeds, and oil has the potential to cure a variety of malady or illness such as gastric ulcer, gout, epilepsy, piles, chronic cough, bronchitis, and arthritis. Some research findings suggest that fenugreek seeds possess antioxidant, anti-inflammatory, antipyretic, antitumor, antimicrobial, and antidiabetic activity. Fenugreek seeds contain various essential phytoconstituents which are responsible for the pharmacological effects. The goal of this study is to explain the preliminary phytochemical standard of fenugreek seeds with powder microscopy and LCMS analysis.
2014
The seeds and aerial parts of Trigonella monspeliaca were analyzed by ICP-MS to determine mineral composition. K, P, Ca and Mg were main minerals in the seeds. Ca, K and P were the major elements in the aerial parts. Total phenol and flavonoid content of the aqueous methanol extracts of the plant was measured by Folin-Ciocalteu and the AlCl3 assay, respectively. Antioxidant potential of the extracts was evaluated by free radical scavenging activity using DPPH assay. The present study showed that the seed extract contained significantly higher amount of total phenolics (150.80±0.33 mg GAE/g) while aerial parts had high amount of total flavonoids. In DPPH radical scavenging assay, inhibition % of the extracts was found to be 51.6-78.0.
2021
Summary Introduction: Natural products represent a gold mine for scientists looking for compounds for the treatment of health problems and diseases with their different biological and pharmacological activities. However, recent research is focused on finding natural sources of antioxidants. Objective: The objective of current research was to determine the phytochemical profile of Algerian fenu-greek (Trigonella foenum-graecum L.), and Syrian cumin (Cuminum cyminum L.) seeds in order to characterize their phenolic compounds and to determine their antioxidant activities. Methods: Total phenolic, flavonoids, condensed and hydrolysable tannins contents were quantified using Folin-Ciocalteu, aluminium chloride, vanillin and ferric chloride methods, respectively. Phenolic compounds were identified by HPLC method and antioxidant activity was measured using DPPH assay. Results: The higher amounts of total phenolic compounds, flavonoids, condensed and hydrolysable tannins were given by fenug...