Estimation of Phytochemicals and Antioxidant potency of Trigonella foenum-graecum Leaves (Variety HM444) (original) (raw)
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Journal of pharmaceutical Sciences and Research, 2019
In the recent era, medicinal weeds are receiving massive attention for their therapeutic efficacy on different oxidative stress-related diseases. In the present study, five common medicinal herbaceous weeds of West Bengal, and India namely; Heliotropium indicum, Tridax procumbens, Cleome rutidosperma, Commelina benghalensis, and Euphorbia hirta have been chosen from five different families e.g., Boraginaceae, Asteraceae, Cleomaceae, Commelinaceae, and Euphorbiaceae, respectively to decipher their phytochemical, and anti-oxidant properties. The research investigations have concluded that Euphorbia hirta possesses a significant amount of phytochemicals, and it exhibits the highest anti-oxidant activities in comparison with the other four medicinal weeds. Euphorbia hirta leaves acetone extract showed the highest content of extractive value (11.88±0.21%), polyphenols (191.28±2.42 mg Gallic acid equivalent/g dry tissue), flavonoids (60.80±1.22 mg Quercetin equivalent/g dry tissue), tannins (17.84±0.23 mg Tannic acid equivalent/g dry tissue), and polysaccharides (206.73±1.37 mg Dextrose equivalent/g dry tissue), that supports their anti-oxidant property, i.e., inhibitory concentration (IC%) value for ABTS (58.57±0.29%), DPPH (59.17±0.45%) and H 2 O 2 (60.17±0.26%) radical scavenging assays. In Euphorbia hirta leaves acetone extract highest amount of phytochemicals were detected by qualitative assays. The bioactive compounds and anti-oxidant activities are well correlated among each other.
2014
The search for sources of potent antioxidants of na tural origin derived from plants is very important i the wake of decreasing resistance of human beings to various di seases. In the present study, antioxidant activity, phenolic contents and phytochemical profile of various extra cts (methanol, chloroform, ethyl acetate and hexane ) of seeds of Trigonella foenum graecum (fenugreek) were investigated. Ethyl acetate extrac t (100 μg/ml) showed highest inhibitory potential with application of DPPH scave nging (69.70%) and chelating power assay (63.44%). The differences in antioxidant activity of extracts are attributed to the presence of various phytochemica ls. The IC50 values of different extracts were also calculated. There was found a positive correlation (R ≥ 0.895) between the total phenolic content and the antioxidant activity of extracts. The phytochemical analysis revealed t h presence of alkaloids, flavonoids, saponins, phenols and tannin s. Results from different parameters...
Annals of phytomedicine, 2023
The leguminous family of plants includes fenugreek (Trigonella foenum-graecum L.), one of the oldest and most promising therapeutic herbs. Fenugreek is a member of the Fabaceae family. The goal of the current study was to evaluate the proximate composition, mineral analysis, phytochemical analysis and antioxidant potency of fenugreek seeds belonging to variety Hisar Mukta (HM) 425. The moisture content (%), crude protein (%), crude fat (%), ash (%), crude fiber (%), total carbohydrates (%) and mineral content (ppm) in fenugreek seeds were evaluated. The acetone extract of seeds was examined for a number of phytochemicals, including total phenolics (1.35 mg GAE/g), total flavonoids (0.12 mg CE/g), total sugars (17.34 mg/g), non-reducing sugars (17.18 mg/g) and reducing sugars (0.16 mg/g). The antioxidant activity was evaluated using the DPPH free radical scavenging activity and the phosphomolybdenum assay. These findings support the notion that fenugreek seeds are an abundant source of phytochemicals and minerals with pharmacological and health benefits.
Asian Journal of Pharmaceutical and Clinical Research, 2020
Objective: The aim of this study is to evaluate the antioxidant potential, determination of total phenolic and flavonoid content in nine selected medicinal plants Spondias pinnata, Melia azedarach, Ageratina adenophora, Urtica dioica, Curcuma longa, Bauhinia variegata, Elaeocarpus angustifolius Blume, Achyranthes aspera, and Psidium guajava from Kavre district of Nepal using in vitro studies. Methods: Methanolic plant extracts were prepared by cold percolation method. The methanol extract of nine medicinal plants collected from Kavre district of Nepal, was screened for assessing bioactive phytoconstituents followed by antioxidant property, total phenolic, and flavonoid content. Different plants collected were powdered and extracted with methanol, concentrated by a rotatory evaporator and analyzed for the presence of phytochemicals. The antioxidant potential of the plant extracts was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. Results: The phytochemical analysis of methanolic extracts of all nine medicinal plants displayed the presence of various secondary metabolites such as alkaloids, flavonoids, polyphenols, saponins, and quinones. The extract of S. pinnata showed the highest percentage of radical scavenging activity up to 87.94±1.88 with 50% inhibitory concentration (IC50) 17.51±1.27 μg/mL, followed by B. variegata, 80.63±1.06 with IC50 value 26.55±2.61 μg/mL. The standard, ascorbic acid has IC50 value of 20.13±1.17 μg/mL. Further, the ethyl acetate fraction of S. pinnata showed the maximum percentage of radical scavenging (85.92±1.37) with IC50 value of 46.95±1.17 μg/mL. Moreover, S. pinnata displayed the highest total phenolic content (TPC) 48.26±1.23 mg GAE/g (milligram gallic acid equivalent per gram) extract while the highest flavonoid content was displayed by Melia azedarach 41.07±1.53 mg QE/g (milligram quercetin equivalent per gram) extract measured by the Folin–Ciocalteu reagent method and aluminum chloride colorimetric method. Conclusions: The preliminary results of this study have put forward the extract of S. pinnata showed the highest percentage of radical scavenging activity and S. pinnata displayed the highest TPC while the highest flavonoid content was displayed by Melia azedarach methanolic extracts although the further studies are needed to assess its mechanism of action
International Journal of Biosciences, 2024
Medicinal plants have been explored for the presence of phytochemicals and natural antioxidants for research and commercial utilization potentials. However, few studies were conducted on the selected medicinal plants' phytochemical profile and antioxidant contents. This study investigated the phytochemical profile, total phenolic, and total flavonoid contents of five methanolic leaf extracts of C. igneus, A. bilimbi, M. charantia, A. esculentus, and B. glabra. All five plants indicated the presence of carbohydrates, saponins, and alkaloids. A. bilimbi, M. charantia, A. esculentus, and B. glabra are potential sources of tannins. The total phenolic content of the extracts varied from 10.17±0.23 to 27.90±0.50 GAE/g. Whereas, the total flavonoid content of these plants ranges from 8.40±0.52 to 87.48±2.42 QE/g. The M. charantia leaves revealed the highest TPC while A. bilimbi yielded the highest TFC among the plants. The selected medicinal plants can be considered good sources of phytochemicals and natural antioxidants, phenolics, and flavonoids, which are the basis for the development and utilization of these plant leaves. The phytochemicals, phenolics, and flavonoids of these plants could be further isolated, purified, characterized, and utilized as antioxidants.
Screening of antioxidant activity and phenolic content of 24 medicinal plant extracts
DARU Journal of Pharmaceutical …, 2008
Background: Antioxidants are vital substances which possess the ability to protect the body from damages caused by free radical-induced oxidative stress. A variety of free radical scavenging antioxidants are found in dietary sources like fruits, vegetables and tea. The purpose of this study was to evaluate the antioxidant activity of methanolic extracts of 24 selected plant materials (seeds or fruits), which are used by Iranian people as folk remedies and/or food supplements. Methods: The antioxidant activity was evaluated against linoleic acid peroxidation using 1,3-diethyl-2-thiobarbituric acid as reagent. At the same time the phenolic content of the extracts was determined using Folin-Ciocalteau reagent to evaluate their contribution to total antioxidant activity. Results: The antioxidant activity expressed as IC 50 ranged from 1.25 µg/ml in cucumber to 167.29 µg/ml in cardamom. Phenolic contents, expressed as gallic acid equivalents, varied from 21.76 mg/100g of the dried weight in linseed to 919.12 mg/100 g of the dried weight in Bishop's weed. No significant correlation was observed between antioxidant activity and phenolic content in the studied plant materials. Conclusion: The results of this study showed that there is no significant correlation between antioxidant activity and phenolic content of the studied plant materials and phenolic content could not be a good indicator of antioxidant capacity.
Oxidants and Antioxidants in Medical Science, 2014
Objective: Reactive oxygen has been implicated in degenerative diseases and medicinal activity of most herbs has been attributed to their ability to scavenge free radicals. Methods: Free radical scavenging and antioxidant activities of ethanolic extracts from the leaves of varieties of medicinal plants, namely Azadiratcha indica, Mangifera indica, Moringa oleifera, Psidium guajava, Terminalia catappa, Anacardiaceae occidentale, Cassia siamae, Chromolaena odorata, Telfaira occidentalis and Paraquetina nigresiens were evaluated. Reducing power, DPPH scavenging, hydroxyl radical scavenging and ferrous ion chelating activities, phenolic, flavonoid and vitamin C contents were determined. Results: C.siamae had excellent DPPH scavenging activity while P.guajava presented the lowest value. T.catappa had peak hydroxyl radical scavenging activity whereas T.occidentalis had the least capacity. C.siamae had the highest total phenolic and flavonoid contents while P.guajava and P.nigresiens showed the lowest phenol and flavonoid values. C.siamae also exhibited the highest reducing power activity whereas P.nigresiens had the least value. P.nigresiens had excellent ferrous iron chelating capacity while C.odorata had only poor activity. The total phenolic and flavonoid contents were highly correlated with the DPPH scavenging and reducing power activities, respectively. M.oleifera had the highest vitamin C content while P.guajava was the least.. Conclusion: Different values were obtained for each parameter for the medicinal leaves and free radical scavenging activity could be attributed to total phenolic and total flavonoid content. Among the plants tested, Cassia siamae leaf extract consistently exhibited the highest antioxidant activity and seems to be a promising source of natural antioxidants.
In the present study, we evaluated antioxidant activity and total flavonoid content of selected medicinally important plant species of India. Extracts of fruits of Rauwolfia tetraphylla, seeds of Nyctanthus arbortristis and leaves of Calotropis gigantea were prepared for the study. In-vitro models like DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging activity was carried out to investigate the antioxidant activity of methanolic plant extracts. Total flavonoid content of the plants was estimated using quercetin as standard to find out their contribution
A comparative study on the antioxidant activity of selected medicinal plant extracts
European Journal of Biotechnology and Bioscience, 2016
The ethanol and methanol extracts of, Prunus dulkis (Almond), Ficus racemosa (Audumbar), Kalanchoe pinnata (Panphutti), Ocimum gratissimum (Tulsi) were screened for their free radical scavenging property using ascorbic acid as standard antioxidant. Free radical sacvanging activity was studied using DPPH method. Highest antioxidant activity observed in Kalanchoe pinnata with 69.841% and 78.56% in ethanol and methanol extract respectively. In case of ethanol extract Ficus racemosa, and Ocimum gratissimum showed higher antioxidant activity as compared to other. In case of methanol extract Kalanchoe pinnata is at top. This present study proves that the plant extracts have considerable antioxidant property and further detailed studies and characterization of these plant extracts can contribute to their medicinal and pharmaceutical applications.