QUANTIFICATION OF CATECHIN IN LEAVES AND STEMS OF MALAYSIAN Uncaria gambir (HUNTER) ROXB. BY HPLC-DAD (original) (raw)
Related papers
2016
Recently, we reported the isolation of a novel flavonoid named uncariechin along with epicatechin and epiafzelechin from the leaf extract of Uncaria longiflora variety pteropoda (Miq.) Ridsd. of the family Rubiaceae. Continuing our investigation on the Uncaria genus, the identification and quantification of its phytoconstituents was carried out. The species of particular interest is the Malaysian Uncaria gambir. This species is distributed mainly in Malaysia and Indonesia and has been cultivated for the flavonoid catechin in Indonesia. Hence, the objective of this study is to determine the quantity of catechin in hexane (Hx), dichloromethane (DCM) and methanol (MeOH) extracts in both stem and leaf parts of the plant via HPLC-DAD. Our findings indicate that catechin is present in higher amounts in the MeOH extract [8.64 % (leaves); 5.12 % (stems)] compared to the DCM extract [0.77 % (leaves); 0.92 % (stems)] with no catechin found in the hexane extract. This is the first report of the quantification of catechin from Malaysian U. gambir using HPLC-DAD. The method can be used for the quantification of flavonoids from other Uncaria and related genus and is useful for targeted isolation of interest flavonoids.
Ethnobotany, Phytochemistry and Pharmacology of Uncaria (Rubiaceae)
ChemInform, 2005
The Uncaria genus is an important source of medicinal natural products, particularly alkaloids and triterpenes. The collected information is an attempt to cover the more recent developments in the ethnobotany, pharmacology and phytochemistry of this genus. During the past 20 years, alkaloids, terpenes, quinovic acid glycosides, flavonoids and coumarins have been isolated from Uncaria. Fifty-three novel structures are reported in this review. The species in which the largest number of compounds has been identified is the Peruvian Uncaria tomentosa or ÔcatÕs claw.Õ Pharmacological studies are described according to cytotoxicity, anti-inflammatory, antiviral, immunostimulation, antioxidant, CNS-related response, vascular, hypotensive, mutagenicity and antibacterial properties. The potential for development of leads from Uncaria continues to grow, particularly in the area of immunomodulatory, anti-inflammatory and vascular-related conditions. The information summarized here is intended to serve as a reference tool to practitioners in the fields of ethnopharmacology and natural products chemistry. (G.B. Hammond). www.elsevier.com/locate/phytochem Phytochemistry 66 (2005) 5-29 PHYTOCHEMISTRY 6 M.E. Heitzman et al. / Phytochemistry 66 (2005) 5-29
Constituents from the Stems of Malaysian Uncaria tomentosa
The Open Conference Proceedings Journal, 2014
Uncaria tomentosa (Willd. ex Schult.) DC. is well known as a medicinal plant that has been used for various treatments. This tropical vine grows up to 30 m tall and commonly found in natural forest. It has hook-like thorns that resemble the claws of a cat. The leaves are elliptic with a smooth edge, and grow in opposite whorls of two. Although there has not been any literature found on the availability of this species locally, we have discovered it in several parts of Peninsular Malaysia. A preliminary investigation of the stems of the plant has yielded three compounds, scopoletin, isopteropodine along with ß-sitosterol. The structures of the isolated compounds were characterized by analysis of spectral characteristics (mostly 1D, 2D NMR and mass spectrometry) and comparison with literature.
Journal of the Brazilian Chemical Society, 2021
The Amazonian Rubiaceae species Uncaria guianensis (UG) is locally used as antiinflammatory, antitumor, antidiabetic, anti-ulcers, and others. The phenolic content of its leaves is characterized by the great predominance of the flavonoid kaempferol-3,7-O-(α)-L-dirhamnoside (kaempferitrin). The present study quantitatively evaluates the kaempferitrin content in the leaves and branches of cultivated and wild UG specimens collected in different locations of the Brazilian Amazon rainforest by employing high-performance liquid chromatography coupled to a diode array detector (HPLC-DAD). Besides, the understanding of the polyphenol profile performed by electron spray ionization is deepened by tandem mass spectrometry analysis (ESI-MS/MS), using a previously approached leaf UG extract, and the flavonoid quercetin-3,7-O-(α)-L-dirhamnoside was first isolated from UG. All samples showed quite similar qualitative polyphenol profiles. Kaempferitrin in UG ranged from 1.1 to 1.9 mg 100 mg-1 for d...
Electroanalytical determination of catechin flavonoid in ethyl acetate extracts of Medicinal Plants
Int. J. Electrochem …, 2009
South African Carpobrotus species have been found to contain hydrolyzable tannins, various flavonoids e.g. rutin and hyperoside, phytosterols and aromatic acids which have a diverse range of pharmacological properties; including antimicrobial and antioxidant activities. In this paper we described the identification and quantification of catechin in C. mellei and C. quadrifidus using cyclic and square wave voltammetry. The oxidation potential of catechin ranges from +171.0 mV to +631.6 mV. The oxidation mechanism proceeds in sequential steps, related to the catechol moiety and 3hydroxyl group. The oxidation of the catechol electron donating group occurs first at low potential and is a reversible reaction. The 3-hydroxyl group oxidises after and undergoes an irreversible reaction. The limit of detection (LOD) of catechin is 0.84 ppm and limit of quantification (LOQ) is 2.79 ppm with R.S.D. equal to 2.5%. The oxidation potentials of the ethyl acetate extracts of C. mellei and C. quadrifidus correspond with that of catechin. The content of catechin in C. mellei and C. quadrifidus was 5.0 ppm and 4.7 ppm, respectively. Other analytical techniques such as HPLC, UV-VIS, NMR, and FTIR were also used to validate the presence of catechin in the plant extracts.
Open Access Constituents from the Stems of Malaysian Uncaria tomentosa
2016
Abstract: Uncaria tomentosa (Willd. ex Schult.) DC. is well known as a medicinal plant that has been used for various treatments. This tropical vine grows up to 30 m tall and commonly found in natural forest. It has hook-like thorns that resemble the claws of a cat. The leaves are elliptic with a smooth edge, and grow in opposite whorls of two. Although there has not been any literature found on the availability of this species locally, we have discovered it in several parts of Peninsular Malaysia. A preliminary investigation of the stems of the plant has yielded three compounds, scopoletin, isopteropodine along with ß-sitosterol. The structures of the isolated compounds were characterized by analysis of spectral characteristics (mostly 1D, 2D NMR and mass spectrometry) and comparison with literature.
Antioxidant Activity of Catechins Isolate of Uncaria Gambier Roxb in Male Rats
LIFE: International Journal of Health and Life-Sciences
The aims of this study were to determine the antioxidant activity of catechins isolates of gambir by measuring the levels of Malondialdehid (MDA) in male white rats. Methods: Catechins of gambir were isolated by using partition method with ethyl acetate solvent. Gambir quality was determined based on National Standard of Indonesia: SNI 01-3391-2000. The yield of catechins obtained from the gambir isolate was determined as (+)-catechins by comparing with standard (+)-catechins and measured by a spectrophotometer UV-VIS at wavelength 279 nm. A total of 25 male rats were divided into 5 groups. Administration of catechins was suspended in 0.5% Na
Jurnal Tumbuhan Obat Indonesia
ABSTRACT Gambir leaves (Uncaria gambir Roxb.) is a plant that has been widely used by people for traditional medicine. The main compound of gambir extract is a (+)-catechin that has been proven as anti-dental plaque, antioxidant, antibacterial, and antihyperlipidemic. This study aims to validate a method for the quantitative determination of catechin in ethyl acetate fraction of gambir extract based on spectrophotometry. The validation was conducted by measuring the linearity, accuracy, and ruggedness of the method that fulfills the requirements along with the limit of detection (LoD) and limit of quantification (LoQ) determination. Determining the precision (based on %RSD and CV), 100 ppm of catechin were made to 7 replicates while accuracy was evaluated by calculating the recovery. The ruggedness of the method is determined by analyzing it on a different day. The linearity was determined by assessing the r-value on the relation between the absorbance curve and standard series c...