Exploiting bacterial isolates for diesel degrading potential under in vitro conditions (original) (raw)
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International Journal for Research in Applied Science and Engineering Technology (IJRASET) , 2021
In this study, we isolated seven strains (termed BY1-7) from polluted soil at an oil station and evaluated their abilities to degrade total petroleum hydrocarbons (TPHs). Among 45 bacterial colonies one bacterial strain was identified based on the cultural, morphological and biochemical characteristics. The isolated bacterium was then subjected to a preliminary assessment of their crude oil after 48 hours of incubation on nutrient agar plates overlaid with 100 ML of petroleum crude oil, the zone of clearance was observed. The isolated bacteria showed 35% petrol degradation, whereas a relatively high oil degradation rate, almost 40% was observed when the bacterium was acclimatized. The selected bacterial strains crude oil resistance was analysed based on the growth ability on the crude oil containing mediums. This strain was identified as Brevibacterium brevis. After inoculation, growth ability was measured and the highest percentage of petrol degradation occurred at temperature 37 °C with the value 30.8%. Bacteria displaying such capabilities are often exploited for the bio-remediation of petroleum oil contaminated environments. Recently, microbial remediation technology has developed rapidly and achieved major gains. However, this technology is not omnipotent. It is affected by many environmental factors that hinder its practical application, limiting the large-scale application of the technology.
The present work was conducted to isolate and identify bacteria from oil- 21 contaminated soil to evaluate their role in biodegradation of commercial diesel under laboratory 22 conditions. Diesel fuels are used by different vehicles, diesel generators and especially heavy 23 transport vehicles. Its manufacturing, transportation, utilization and disposal have the threat to 24 pollute the surrounding environment. Biodegradation is one of the biological processes to 25 remediate the pollutants. This is the cheaper and easy method as compared to other methods like 26 direct burning, land foaming and bioventing because diesel smoke has a carcinogenic effect. 27 In the current exploration ordinary bacterial species that degrade diesel were isolated from 28 selected areas of Mansehra. Six sites were selected and a total of 60 samples were collected. 29 These sites were coded according to their names. For the isolation of diesel degrading bacteria 30 soil samples were analyzed. The isolation of bacteria was done on nutrient agar, nutrient broth 31 and mineral salt medium (MSM), and IR spectroscopy was used to observe the degradation of 32 diesel. Twenty samples were found having bacterial growth. Further analysis showed that four 33 samples BHG, SHNK, TAK and KMOR had diesel degrading capability as these showed 34 positive results. The isolated bacteria were identified by morphological and biochemical features, 35 while the degradation of diesel was determined qualitatively by interpreting the intensity of the 36 peaks of IR spectroscopy. The intensity of the peaks in the spectrum of control was compared 37 with the intensity of the peaks in the spectrum of the test samples which indicated degradation of 38 diesel. Three bacterial species, namely, Bacillus cereus, Bacillus subtilis and Pseudomonas 39 aeruginosa were identified using Berge's manual method. The bacterial species were tested for 40 their capability to degrade commercial diesel presented at different concentrations i.e. 1000 ppm 41 and 5000 ppm.
Isolation and Identification of Effective Hydrocarbon Degrading Bacteria from Diesel Polluted Soil
IOSR Journal of Biotechnology and Biochemistry, 2017
Hydrocarbon pollution is due to accidental leakages, spills or by transportation. It might be carcinogenic to human, animal and plant health. Bioremediation is the best remedy to remove the pollutants from the environment. Diesel degrading microbes are widely distributed in our environment. The microbes may not be present in sufficient amount to achieve the degradation process in successfully. Inoculation of efficient hydrocarbon degrading bacterial strains in polluted area will enhance the capability of degradation. The present study was aimed to be isolating the effective bacterial strains from the polluted area to enhance the degradation. Totally fifteen isolates were identified and biochemically characterized. Among these two of them were effective degrader under 4% diesel. Pseudomonas putida and Micrococcus luteus are the bacterial strains were able to degrade the contaminants in large amounts. In this study we report the bacterial strains capable of degrading the hydrocarbons efficiently. Degradation studies to be carried out with different isolates at different concentration of diesel to find out most potent hydrocarbon degrading strains, which can be used for any bioaugmentation studies during bioremediation
Isolation and Identification of Diesel oil-degrading Bacteria in used engine oil contaminated soil
Journal of Applied Science and Environmental Management, 2019
This study was conducted to isolate and identify bacteria associated with spent diesel oil contaminated site by collecting three replicates contaminated soil from the works department of Admiralty University of Nigeria, while the pristine soil was obtained from the biological garden. pH and temperature of all the samples were checked and the bacteria isolates were identified by morphological and biochemical techniques using the taxonomy scheme of Bergey's Manual of determinative bacteriology. The pH and temperature was highest (6.06 to 6.26) and (27 to 30 0 C) in the contaminated soil than the pristine soil (5.83) and (23 0 C). The mean microbial load count was highest (10.0x10 5 cfu/ml)in the diesel oil contaminated soils than the pristine uncontaminated soils (9.0x0x10 5 cfu/ml). However, there was no significant difference between the microbial loads in the soil samples. Eight bacteria species were obtained from the contaminated soil while six were obtained from the pristine soil. Klebsiella spp., Enterobacterae riginosa, Pseudomonas sp. and Escherichia coli showed highest growth in the contaminated soil and therefore may be considered as effective in the degradation of spent diesel oil hydrocarbons. It is likely that these bacteria when employed independently may be used to degrade hydrocarbons. Further research is necessary to assay for the degradative ability of the microorganisms.
Diversity of diesel degrading bacteria from a hydrocarbon contaminated soil
2014
Hydrocarbon contamination resulting from leaking above ground and underground storage tanks, spillage during transport of petroleum products, abandoned manufactured gas sites and various industrial processes is hazardous to soil and water ecosystem and is expensive to remediate. There is an increased interest in promoting ecofriendly methods in the process of cleaning oil-polluted sites. Biological and non-biological approaches are being used for remediation of oil pollution. Bioremediation is one of the principle strategies for remediation, wherein the pollution can be removed by use of microorganism or by any biological process that uses microorganisms or their enzymes to return the environment altered by contaminants to its original condition (OluArotiowa et al., 2007).
Isolation and Characterization of Diesel Degrading Bacteria from Petroleum Oil Contaminated Soil
Bacterial Empire
Petroleum products are used for energy production and an essential part of our day-to-day lives especially in vehicles, ships, and industries. Accidental leakages occur easily and wastage petroleum is also discarded in the environment without any further processing causing environmental pollution. Diesel contributea big part topetroleum pollution. The current study was aimed to identify diesel degrading bacteria and determine some conditions to evaluate their best degradation capability. We identified Aeromonas spp., Bacillus spp., and Enterobacter spp. from diesel contaminated soil and found that Aeromonas spp. and Bacillus spp. grow best with 10% to 15% diesel whereas Enterobacter spp. can grow quite well with 20% diesel concentration at a higher temperature (40oC) than the previous two bacteria. Aeromonas spp. worked well at low pH (pH 4 to pH 6) whereas Bacillus spp. and Enterobacter spp. worked best at higher pH (pH 10).
The present study was undertaken to isolate and characterize the hydrocarbon degrading microbes from oil contaminated soil samples in Coimbatore, Tamilnadu, India. Totally 113 bacterial isolates were obtained from 60 soil samples. Four were finally selected through secondary screening by an agar well diffusion method using Benzene, Toluene and Xylene (BTX) as carbon source. They were provisionally identified and found closely related to Bacillus cereus, Micrococcus spp, Pseudomonas aeruginosa and E. coli. These isolates selected based on the highest zone of the inhibition on agar well diffusion method. Furthermore, 4 potentials isolate were subjected to gravimetric analysis for determinate the degrading capacity of hydrocarbon degradaters. in this study, Bacillus cereus were degrade the highest amount of BTX, especially Benzene was highly degrade and followed by Toluene. This study clearly demonstrates that Gram-positive bacteria are effective in BTX degradation.
Biodegradation of Diesel Oil in Soil by a Microbial Consortium
Water Air and Soil Pollution, 2001
Biodegradation of diesel oil was performed using adiesel oil-degrading bacterial consortium, in bothlaboratory and pilot scale experiments. The bacterialconsortium was prepared in liquid for laboratory testsand for pilot scale experiments, it was prepared intwo steps, liquid and then in soil. The concentrationof diesel in soil treated with the bacterialconsortium was reduced to <15% of the initialconcentration, within a period of five weeks in bothlaboratory (135 to 19.32 g diesel kg (soil dryweight)-1) and pilot scale (118 to 17.5 g dieselkg (soil dry weight)-1) experiments, incomparison with controls (without bacterialconsortium), in which initial concentration of dieselwas reduced by only 5 and 15%, respectively. Dieselbiodegradation rate with the bacterial consortium was2.13 g diesel kg (soil dry weight)-1 d-1, itwas slightly enhanced by the addition ofNH4NO3 in the presence of bacterialconsortium 2.78 g diesel kg (soil dry weight)-1d-1. The enhancement of the microbial activity inhydrocarbon-contaminated soil can be achieved with thecombination of stepwise soil inoculation and nutrient additions.
Isolation and Enrichment of Microbes for Degradation of Diesel Oil
Oil refinery proves to be chief source of environmental pollution, which leads to human health hazards and damage soil surroundings. The present investigation aims to isolate petroleum tolerant and degrading microbes from oil contaminated soil of oil refinery, which later be used in environmental biotechnology as remediating mechanisms. Bushnell Haas Media (BHM) supplemented with diesel oil as carbon and energy source, used for isolation of oil degrading bacteria. The degraders were analyzed by streak plate method and bacteria were isolated by the selective enrichment culture plate technique and the biodegradation potential was determined.