Phenotypic detection of Extended Spectrum Beta lactamase production from E. coli and K. pneumoniae in urinary samples among children (original) (raw)
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Journal of Shaheed Suhrawardy Medical College, 2012
Background: Infections due to extended spectrum β-lactamases (ESBL) producing Escherichia coli and Klebsiella pneumoniae have become an important clinical problem. These organisms are important regarding the infection control by the physicians. Objective: The present study was undertaken to determine the prevalence of ESBLs along with their antimicrobial sensitivity pattern in Escherichia coli and Klebsiella pneumoniae. Methodology: This cross sectional study was conducted in the Department of Microbiology at Sir Salimullah Medical College, Dhaka. Urine samples were collected from patients who were clinically suspected to have UTI. After incubation, plates were checked for presence of suspected pathogens. Organisms were identified to species level by conventional methods. All isolated E. coli and K. pneumoniae were included in the study. The susceptibility to antibiotics was determined by Kirby Bauer method on Muller Hinton agar. Isolates were screened for ESBL production by using disk diffusion of cefotaxime, ceftazidime, ceftriaxone and cefpodoxime placed on inoculated plates containing Muller Hinton agar according to the CLSI recommendations. Phenotypic confirmatory test for ESBL producers was done by combined disc diffusion for all the isolates that were screened positive for the ESBL production following CLSI guidelines. Combined disk diffusion method was also done in this study. Result: A total of 220 non repeated urine samples were cultured of which 132(60%) cases had shown the bacterial growth. Among the 132 samples Escherichia coli had found in 103(78.0%) cases and Klebsiella spp. was found in 14(10.6%) cases. Out of 103 E coli 23(22.3%) cases was found as ESBL strain. On the other hand within 14 Klebsiella species, the ESBL strain was found in 5(35.7%) cases. Both E coli and Klebsiella species were 100% sensitive to imipenem. However, cephamycin was sensitive in 93.7% and 100% in E coli and Klebsiella species respectively. Conclusion: Results indicate that routine ESBL detection should be made imperative and empirical use of third generation cephalosporins must be discouraged.
International Journal of Biological Chemistry
Multidrug resistant strains of Klebsiella pneumoniae and Escherichia coli constitute a problem in many hospitals. The antibiotic susceptibility profile and the Production of Extended Spetrum Beta-Lactamase (ESBL) of the strains of Klebsiella pneumoniae and Escherichia coli were assessed by the Kirby-Bauer technique and the modified disc diffusion technique respectively. Out of a total of 65 urinary specimens submitted to the Medical Microbiology laboratory at the Ahmadu Bello University Teaching Hospital, Shika-Zaria, Nigeria, 50 isolates consisting of 33 (66%) Escherichia coli and 17 (34%) strains of Klebsiella pneumoniae were recovered from patients suspected to have Urinary Tract Infections (UTIs). Of these 50 isolates, 15(30%) were ESBL producers, made up of 6/17 (35.3%) ESBL-positive Klebsiella pneumoniae isolates and 9/33 (27.3%) ESBL-positive E. coli isolates. The susceptibility of the ESBL-positive Klebsiella pneumoniae isolates to ciprofloxacin, ofloxacin and amikacin were ...
BMC Research Notes, 2013
Background: Escherichia coli and Klebsiella pneumoniae are the major bacterial pathogens being isolated and reported from mid stream urine (MSU) specimens, globally. These uropathogens are mostly implicated as the major extended spectrum beta-lactamase (ESBL) producers, severely limiting the therapeutic management in cases of urinary tract infections. Limited studies had been reported from Nepal investigating the ESBL producers among uropathogens. This study was designed to assess the prevalence of ESBL producing E.coli and K. pneumoniae in urinary isolates at a centrally located major tertiary care hospital in Kathmandu valley, Nepal. Methods: Between September 2011 and May 2012, during the nine months period, 6308 MSU specimens were collected aseptically from the same number of clinically suspected patients of urinary tract infections. The samples were cultured on MacConkey agar and blood agar. The isolates with significant bacteriuria (10 5 CFU / ml) were identified based on standard laboratory procedures. Antimicrobial susceptibility tests were carried out using various antimicrobial discs alongwith ceftriaxone on E.coli and K. pneumoniae isolates by Kirby Bauer disc diffusion method as per the recommendations of CLSI. On initial screening with ceftriaxone (30 μg) disc showing resistance was then confirmed for ESBL production by phenotypic confirmatory disc diffusion test (PCDDT) using ceftazidime (30 ug) and ceftazidime + clavulanic acid (30 μg + 10ug) disc as per guidelines of CLSI (2011). Results: Out of a total of 6308 MSU specimens investigated for significant bacteriuria, E.coli isolates were 444 (7.04%) and K.pneuminiae were 145 (2.3%) making a total of 589 (9.34%). Initial screening with ceftriaxone disc revealed 155 isolates of E.coli and 70 isolates of K.pneumoniae to be resistant. Further testing by PCDDT method showed 60/444 (=13.51%) of E. coli and 24/145 (=16.55%) of K. pneumoniae isolates to be confirmed ESBL producers. These ESBLproducer uropathogens showed high degree of resistance to ceftriaxone (100.0%), amoxycillin, fluoroquinolones and co-trimoxazole. Conclusion: An emerging and moderately high prevalence of ESBL-producing E. coli and K. pneumoniae was observed and confirmed in the urinary isolates investigated. It is essential to have a regular and routine monitoring of ESBL producing clinical isolates in laboratory practice.
Indian J Med Microbiol, 2004
Introduction: Urinary Tract infections caused by Escherichia coli have become a significant global public health problem. The resistance to βlactam antibiotics in these clinically important gram negative bacteria further adds to the problem. Knowledge about their prevalence is essential to guide towards appropriate antibiotic treatment. The study was thus undertaken to know prevalence and susceptibility pattern of Extended spectrum β-lactamase (ESBL) producing Escherichia coli isolates among urinary samples. Methods: A total of 216 E. coli isolates in urinary samples were studied for ESBL production by using CLSI Phenotypic screening and confirmatory tests. Results: 53% of E.coli isolates were ESBL producers. The susceptibility of ESBL producers to Imipenem, Tigecycline and Nitrofurantion was found to be 100%, 100% and 88% respectively. A high degree of associated resistance to Amikacin, Co-trimoxazole and Quinolones was found in ESBL producers. Majority of ESBL producers were detected among patients admitted in various ICUs and surgery ward. Conclusion: Our study shows presence of ESBL producer E.coli in large number of urinary isolates.
FUDMA Journal of Sciences, 2019
Klebsiella pneumoniae and Escherichia coli are the major bacterial pathogens being isolated and reported from urine samples worldwide as Extended Spectrum Beta Lactamases (ESBLs) producers. This study was conducted to determine the prevalence of ESBLs producing K. pneumoniae and E. coli from urinary clinical isolates in Federal Teaching Hospital Gombe. A total of two hundred and nine (209) bacterial isolates comprising 100 E. coli and 109 K. pneumonia were collected and recovered on MacConkey agar at 35 o C. The isolates were further identified as members of Enterobacteriaceae family using conventional biochemical test. Preliminary screening for ESBL production was carried out using antibiotics disk of ceftadizime (30µg), ceftriazone (30µg) and cefpodozime (10µg) placed on to a surface of Muller-Hinton agar plates, separated from each other by a distance not less than 20mm apart, zones of growth inhibition were measured to the nearest mm. The screened isolates were further confirmed...
https://www.ijhsr.org/IJHSR\_Vol.6\_Issue.3\_March2016/16.pdf, 2016
Objectives: Resistance to third generation cephalosporins in E. coli and K. pneumoniae are due to various factors. Present study was an attempt to detect resistance mediated by beta-lactamases production. Methods: A total of 300 uropathogenic E. coli and Klebsiella pneumoniae isolates were further subjected to detection of beta-lactamases by disc diffusion method. Result: A total of 300 urinary isolates were studied, out of these 189 isolates were beta-lactamases producer. Out of 189, E. coli shown to have beta-lactamases production in 144 isolates while 45 isolates of K. pneumoniae were beta-lactamases producer. Conclusion: The prevalence of resistance mediated by beta-lactamase production is increasing day by day and varies from different geographical areas; hence it should be mandatory to detect beta-lactamases on routine basis.
Tribhuvan University Journal of Microbiology
Objectives: The study was aimed to determine the prevalence of Extended Spectrum Beta Lactamase (ESBL) producing Gram negative pathogens from urine samples along with their antimicrobial resistance. Methods: This cross-sectional study was conducted from December 2015 to May 2016 at Everest Hospital, Kathmandu. Mid-stream urine samples were collected and processed for culture by standard loop streak method. Identified bacterial isolates were tested for Antibiotic Susceptibility by modified Kirby Bauer disc diffusion method and, were subjected to ESBL screening by using 30µg cefotaxime and ceftazidime. ESBL production was confirmed by combination disc method. Results: Of the three hundred urine samples, 22.7% (67/300) showed significant growth. Four different bacterial species were identified. Among the isolates, E. coli was the most common pathogen (71.64%) followed by Klebsiella pneumoniae (14.92%), Pseudomonas spp (8.95%) and Acinetobacter spp (4.48%). Altogether 92.54% (n=62) isol...
Journal of Health and Allied Sciences
This study was designed to determine the distribution of bacterial strains isolated from patients with urinary tract infections (UTI) at Sukrararj Tropical and Infectious Disease Hospital (STIDH) with special reference to ESBL, ABL and the co-existence of the phenotype (ESBL and ABL in the population of different uropathogens. Urine samples from 500 patients suspected of UTI were analyzed by conventional semi-quantitative culture technique. The antimicrobial susceptibility to various drugs was studied by the Modified Kirby-Bauer disk diffusion method, by following the ClSI guidelines. Confirmation of the extended spectrum β-lactamase (ESBL) and AmpC β-lactamase production was done by the phenotypic confirmatory disc diffusion test (PCDDT) and the AmpC disk test (ADT). Overall, the prevalence of ESBL and ABL and the co-existence of the phenotype (ESBL and ABL) in urinary isolates were found to be 39.22%, 15.69% and 5.88%. The implementation of appropriate infection control measures a...
2020
Background: Resistance of Gram-negative bacteria to antibiotics had increased at an alarming pace over the last two decades. Empirical antibiotic treatment of UTI practice created opportunity for frequent use of antibiotics that resulted in emergence of resistant strains. Extended spectrum beta-lactamase producing Enterobacteriaceae are emerging worldwide and are usually multi-drug resistant; thus, limiting treatment options for these organisms. Hence, it is mandatory to scrutinize the extent of the problem in the study area. Objective: We investigated the prevalence and correlates of extended spectrum beta-lactamase producing Enterobacteriaceae and their antibacterial resistance pro le among children with urinary tract infection (UTI) at Hawassa University Comprehensive Specialized Hospital. Method: A hospital-based cross sectional study was conducted from February 1-July 30/ 2018. A semi-structured questionnaire was used to collect data from 284 children suspected of UTI. Bacterial species were isolated using standard bacteriological techniques. Antibiotic susceptibility testing was done using Kirby-Bauer disk diffusion method and ESBL production was detected by double disc synergy test. Data entry, cleaning and statistical analysis was done using SPSS version 20. Results: Overall, Enterobacteriaceae accounted for 75% (72/96) of UTI infections. The most frequent Enterobacteriaceae were E. coli , 44.4% (32/72) and K. pneumoniae , 27.8% (20/72). Overall, ESBL-producers within Enterobacteriaceae accounted for 41.7% (30/72) and magnitude of multi-drug resistance level was 86.1% (62/72). ESBL producing K. pneumoniae and E. coli accounted for 70% (14/20) and 37.5% (12/32), respectively of all the respective isolates. Previous UTI within the past 12 month (AOR= 0.08, 95% CI (0.01-0.57) and medium family wealth index (AOR = 0.03, 95% CI (0.00-0.27) were associated with infection of ESBL-producing Enterobacteriaceae. Conclusion: ESBL producing Enterobacteriaceae were prevalent among UTI cases; where E. coli and K. pneumoniae were the major isolated resistant strains that contributed mainly to the problem. Majority of Enterobacteriaceae isolated were resistant to commonly prescribed drugs.