Bacterial Production of PHAs from Lipid-Rich by-Products (original) (raw)
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Animal fat and glycerol bioconversion to polyhydroxyalkanoate by produced water bacteria
e-Polymers
Oil reservoirs contain large amounts of hydrocarbon rich produced water, trapped in underground channels. Focus of this study was isolation of PHA producers from produced water concomitant with optimization of production using animal fat and glycerol as carbon source. Bacterial strains were identified as Bacillus subtilis (PWA), Pseudomonas aeruginosa (PWC), Bacillus tequilensis (PWF), and Bacillus safensis (PWG) based on 16S rRNA gene sequencing. Similar amounts of PHA were obtained using animal fat and glycerol in comparison to glucose. After 24 h, high PHA production on glycerol and animal fat was shown by strain PWC (5.2 g/ L, 6.9 g/ L) and strain PWF (12.4 g/ L, 14.2 g/ L) among all test strains. FTIR analysis of PHA showed 3-hydroxybutyrate units. The capability to produce PHA in the strains was corroborated by PhaC synthase gene sequencing. Focus of future studies can be the use of lipids and glycerol on industrial scale.
Microbial Production of Polyhydroxyalkanoates by Bacteria Isolated from Oil Wastes
Applied Biochemistry and Biotechnology, 2000
A Gram-positive coccus-shaped bacterium capable of synthesizing higher relative molecular weight (M r) polyhydroxybutyrate (PHB) was isolated from sesame oil and identified as Staphylococcus epidermidis (by Microbial ID, Inc., Newark, NJ). The experiment was conducted by shake flask fermentation culture using media containing fructose. Cell growth up to a dry mass of 2.5 g/L and PHB accumulation up to 15.02% of cell dry wt was observed. Apart from using single carbohydrate as a sole carbon source, various industrial food wastes including sesame oil, ice cream, malt, and soya wastes were investigated as nutrients for S. epidermidis to reduce the cost of the carbon source. As a result, we found that by using malt wastes as nutrient for cell growth, PHB accumulation of S. epidermidis was much better than using other wastes as nutrient source. The final dried cell mass and PHB production using malt wastes were 1.76 g/L and 6.93% polymer/cells (grams/gram), and 3.5 g/L and 3.31% polymer/cells (grams/gram) in shake flask culture and in fermentor culture, respectively. The bacterial polymer was characterized by 1 H-nuclear magnetic resonance (NMR), 13 C-NMR, Fourier transform infrared, and differential scanning calorimetry. The results show that with different industrial food wastes as carbon and energy sources, the same biopolymer (PHB) was obtained. However, the use of sesame oil as the carbon source resulted in the accumulation of PHB with a higher melting point than that produced from other food wastes as carbon sources by this organism under similar experimental conditions.
Journal of Polymers and The Environment, 2010
A consortium of microorganisms from oil polluted wastewater sample was cultivated to promote polyhydroxyalkanoate (PHA) accumulation before subjecting the mixed cultures to sucrose density gradient ultracentrifugation. This resulted in the fractionation of the bacterial cells according to their physical features such as size, morphology and/or densities. An isolate was identified as Burkholderia sp. USM (JCM15050), which was capable of converting palm oil products [crude palm kernel oil (CPKO), palm olein (PO), palm kernel acid oil (PKAO), palm stearin (PS), crude palm oil (CPO), palm acid oil (PAO) and palm fatty acid distillate (PFAD)], fatty acids and various glycerol by-products into poly(3-hydroxybutyrate) [P(3HB)]. Up to 70 and 60 wt% of P(3HB) could be obtained when 0.5%(v/v) CPKO and glycerol was fed, respectively. Among the various fatty acids tested, lauric acid followed by oleic acid and myristic acid gave the best cell growth and PHA accumulation. Compared to Cupriavidus necator H16, the present isolate showed better ability to grow on and produce PHA from various glycerol by-products generated by the palm oil industry. This study demonstrated for the first time an isolate that has the potential to utilize palm oil and glycerol derivatives for the biosynthesis of PHA.
Bacterial Production of Hydroxyalkanoates (PHA)
Universal Journal of Microbiology Research, 2016
The dependence of plastic materials is an increasing problem. Although plastics are very useful for humankind many disadvantages derived from the difficulties linked to recycling and disposal are well known. A feasible alternative is the production and use of bioplastics. These compounds have multiple options at the end of his life that can ensure their safety and efficacy of reuse or recovery. For example, raw materials can be returned to the manufacturer for recycling. Bioplastics synthesized through biotechnology include mainly polyhydroxyalkanoates (PHAs), common lipoidic storage materials accumulated by prokaryotes. Some processes for producing PHAs by fermentation using microorganisms have been developed at a different extent. However, biopolymers (PHA) market is under development, and therefore cannot compete with traditional plastics since manufacturing is still more expensive. In this review we have focused on the study of the production processes of bioplastics where bacteria are present, also describing the scaling to industrial level aspects and future trends.
Environmental biotechnology has the intention of increasing sustainability of production processes by employing biological systems and thereby benefiting the environment. Microorganisms are a biological system which is generally used for the reduction of pollution from air, aquatic or terrestrial systems. Edible oil and fats are utilized by microorganisms and produces new product such as lipase and biodiesel was investigated. In present study the microorganisms utilizing edible oil as carbon source were isolated and investigation of their characteristics towards the production of Polyhydroxyalkanoates (PHA), which is now a days well known as Biodegradable polymer. Sixteen bacterial colonies were isolated, screened by providing various edible oils as carbon source and preserved using glycerol. The microorganism then stained for PHA with Sudan Black B stain. We have found that nine out of sixteen strains exhibited PHA producing ability. The organisms were identified through several bi...
Production of Polyhydroxyalkanoates from Renewable Sources Using Bacteria
Journal of Polymers and the Environment, 2018
Plastics play a very important role in our daily life. They are used for various purposes. But the disposal of these petrochemical-derived plastics causes a risk to the human and marine population, wildlife and environment. Also, due to the eventual depletion of petrochemical sources, there is a need for the development of alternate sources for the production of plastics. Biodegradable polymers produced by microorganisms can be used as substitutes for conventional plastics derived from petrochemical sources since they have similarity in their properties. Polyhydroxyalkanoate (PHA) is one such biopolymer that will be accumulated inside the cells of microorganisms as granules for energy storage under limiting conditions of nutrients and high concentration of carbon. Research on the microbial production of PHA should focus on the identification of costeffective substrates and also identification of a suitable strain of organism for production. The major focus of this review is the production of PHA from various cost-effective substrates using different bacterial species. The review also covers the biosynthetic pathway of PHA, extraction method, characterization technique, and applications of PHA in various sectors.
Trends in Sciences
Polyhydroxyalkanoates (PHAs) are a group of biopolymers used as an alternative to petroleum-based synthetic plastics. Their industrial application is not widely available due to production cost constraints, mainly from raw materials used for carbon sources. This research focuses on selecting a Bacillus strain from lipid-containing wastewater that can produce PHAs from crude glycerol and investigating the optimum conditions for producing PHAs from crude glycerol. The Bacillus strain was isolated from the wastewater storage pond at a fermented pork sausage manufacturing plant in Thailand for PHAs production utilizing Mineral Salt Medium (MSM) with 1 % w/v glucose as a carbon source. PHAs synthesis was preliminarily investigated by staining cells with Sudan Black B. The isolated FMI3 produced the highest PHAs at 43 % of the dry cell weight (DCW). It was identified as Bacillus pumilus FMI3. Crude glycerol was obtained from a by-product of the transesterification of waste cooking oil cat...
2018
Polyhydroxyalkanoates (PHAs) is a bio-polymer accumulated in a bacterial cell. PHA is a potential substitute in fuel industry, medical industry and material industry such as biofuel, surgical pins and latex. This work is to isolate PHA producing bacteria from sources, including contaminated soil, industrial wastewater and domestic wastewater in Bangkok Thailand. Total 250 strains screening for PHA production using Sudan black B, Nile blue A and Nile red staining methods. After, preliminary staining 9 isolates exhibiting high PHA production were confirm by High Pressure Liquid Chromatography (HPLC). The Strain KU G15 was selected for further analysis due to its high PHA production at 11.69 % by cell dry weight. Effect of carbon sources and nitrogen sources on growth and PHA production of strain KU G15 were investigated. Strain KU G15 was grown on mineral salt medium supplemented with different carbon sources (glycerol, palm oil, starch, glucose, sucrose, fructose, maltose, and lactos...
Screening of PHA-Producing Bacteria Using Biodiesel-Derived Waste Glycerol as a Sole Carbon Source
Journal of Water and Environment Technology, 2010
Different sources of wastewater and soil were used to screen for PHA-producing bacteria using biodiesel-derived waste glycerol as a sole carbon source by the Nile red staining method together with polymer determination. Twelve out of twenty-six isolates from biodiesel-contaminated wastewater consortium were screened for their PHA accumulation ability by cultivation in mineral salt medium supplemented with waste glycerol. The AIK7 isolate was chosen as a potential PHA producer. The PHA production on waste glycerol was examined using pure glycerol as a control substrate. The PHA content of AIK7 isolate cultivated in 10 g/L glycerol could reach 35% cell dry weight in 72 hours from waste glycerol and 33% cell dry weight in 120 hours from pure glycerol cultivation. It can be seen that at this content of waste glycerol, AIK7 isolate is effectively capable of biotransforming glycerol into polymer from low-grade glycerol.