Simultaneous Multicomponent Spectrophotometric analysis of Ampicillin and Probenecid in Pharmaceutical formulation by Derivative spectroscopy (original) (raw)
Related papers
International Journal of Advances in Scientific Research, 2015
Simple, fast and reliable spectrophotometric methods were developed for determination of Ampicillin Trihydrate in bulk and pharmaceutical dosage forms. The solutions of standard and the sample were prepared in Methanol. The quantitative determination of the drug was carried out using the second order Derivative Area under Curve method values measured at 224-231nm. Calibration graphs constructed at their wavelengths of determination were linear in the concentration range of Ampicillin Trihydrate using 5-25μg/ml (r²=0.997) for first order Derivative Area under Curve spectrophotometric method. The proposed methods have been extensively validated as per ICH guidelines. There was no significant difference between the performance of the proposed methods regarding the mean values and standard deviations. The developed methods were successfully applied to estimate the amount of Ampicillin Trihydrate in pharmaceutical formulations.
Spectrophotometric determination of ampicillin and amoxicillin
A simple, accurate and sensitive spectrophotometric method is described for the determination of ampicillin and amoxicillin in pure forms and in pharmaceutical formulations. The method is based on the reaction of these drugs in aqueous solution with tetracyanoethylene reagent in the presence of sodium hydroxide to produce a yellow coloured species measurable at λmax 322 and 325nm for ampicillin and amoxicillin respectively. The method has been used for the determination of 1.0-40 µg/ml for both drugs. The complexes have apparent molar absorptivities of 7.545×103 and 9.915×103 L.mol-1.cm-1 and Sandell sensitivities of 0.0532 and 0.042 µg.cm-2 for above drugs respectively. Also, it was found that these products were formed in ratio of 1:1 species with stability constants of 7.96104 and 3.91104L/mol for ampicillin and amoxicillin respectively. The method was applied successfully to the assay of ampicillin and amoxicillin in their pharmaceutical formulations and was agreed well with its certified value and with the standard addition procedure.
Journal of Pharmaceutical and Biomedical Analysis, 1998
A simple spectrophotometric method is used for the resolution of the binary mixtures of ampicillin sodium and sulbactam sodium. In aqueous solution, zero-order spectra are subject to interference, so first-derivative spectrophotometry was used to enhance the spectral details allowing the determination of ampicillin sodium from the signal at the zero-crossing point for sulbactam sodium at 268 nm. In 0.1 N sodium hydroxide, sulbactam sodium was determined from the absorbance at 260 nm with negligible contribution from ampicillin sodium. Also, sulbactam sodium was determined without interference using first- and second-derivative spectra in 0.1 N sodium hydroxide at 276 nm (peak-height) and 262–284 nm (peak-to-peak), respectively. The method is rapid, simple, does not require a separation step and allows the determination of each drug without interference from the other. The proposed method has been applied successfully to the assay of these drugs in mixtures and in commercial injections.
Spectrophotometric Determination of Ampicillin with Sulfanilic Acid by Oxidative Coupling Reaction
Indian journal of forensic medicine and toxicology, 2020
Sensitive, economic, simple and accurate spectrophotometric method for estimation of ampicillin in bulk and dosage form is described. Ampicillin was oxidized by sodium hypochlorite and coupling with sulfanilic acid in the presence of sodium hydroxide to obtain a stable yellow colored chromogen which exhibit a maximum absorption (λ max) at 400 nm. The optimum conditions were carefully evaluated. Plot of absorbance against concentration was linear over the range (50-300 µg.ml-1). This method was applied to the estimation of ampicillin in pure drug and commercial formulations successfully.
2014
The objective of the study was to develop a simple, accurate, precise and rapid UV spectrophotometric, second order derivative method for validation of amoxicillin and carbocisteine. The validation was carried out by using ICH guidelines for the determination of amoxicillin and carbocisteine by using 0.1 N hydrochloric acid as the solvent in combined dosage form. The proposed second order derivative method involves the measurement of absorbance of one drug at zero crossing point of other; hence, wavelengths 241.8 nm and 208 nm were selected for the estimation of amoxicillin and carbocisteine, respectively. The linearity of the proposed method was found in the concentration range of 20 to 100 µg/mL (r 2 = 0.9998) for amoxicillin and 10 to 100 µg/mL (r 2 = 0.9988) for carbocisteine, respectively. The percentage mean recovery was found to be 100.129% for amoxicillin and 100.163% for carbocisteine, respectively. The method was also statistically validated for its linearity, accuracy and...
European Chemical Bulletin, 2013
Simple, precise and accurate visible spectrophotometric procedure was developed for the quantitative determination of two antibacterials, ampicillin and cloxacillin in a fixed dose combination. The method exploits the formation of charge transfer complexes between the antibacterials and 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ) to determine the components of the drugs in a dosage form. The effect of different variables on the development and stability of the complexes were studied and optimized. Beer's law was obeyed. The method was successfully applied to the determination of the target analytes in different brands of the fixed dose combination.
SPECTROPHOTOMETRIC DETERMINATION OF AMOXICILLIN IN PHARMACEUTICAL FORMULATIONS
In present study, three new spectrophotometric methods, original UV spectrophotometry, first and second order derivative UV spectrophotometry, were developed for the determination of amoxicillin in pharmaceutical preparations. In original UV spectrophotometry, absorbances were measured at 247.0 nm in the zero order UV spectra of the solution of amoxicillin in 0. 1N NaOH in the range of 220 -350 nm. In first derivative UV spectrophotometry, dA/dX values were measured at 255.8 nm in the first derivative UV spectra of the solution of amoxicillin in 0. IN NaOH in the range of 220 -320 nm (Ak= 2 nm). In second derivative UV spectrophotometry a^A/dl 2 values were measured at 249.2 nm in the second derivative UV spectra of the solution of amoxicillin in 0. IN NaOH in the range of 220 -320 nm (AX= 4 nm). Linearity range was found as 3.2 -48.0 /ug/mL in all three methods. Mean recoveries and the relative standard deviations of the methods were found as 99.67 % and 1.20 % in original UV spectrophotometry at 247.0 nm, 99.04 % and 1.76 % in first derivative UV spectrophotometry at 255.8 nm and, 99.43% and 2.34 % in second derivative UV spectrophotometry at 249.2 nm respectively. Three spectrophotometric methods developed were successfully applied to 8 tablets, 2 oral suspensions and 1 lyophilized powder formulation commercially available in Turkish drug market. All the results were compared statistically with those obtained by using the methods indicated in USP XXIII Amoksisilin'in farmasotik preparatlarda spektrofotometrik miktar tayini Bu qalismada, amoksisilin'in farmasotik preparatlarda miktar tayini iqin uq yeni spektrofotometrik yontem, orijinal UV spektrofotometri, birinci ve ikinci turev spektrofotometri, gelistirilmistir. Orijinal UV spektrofotometride absorbans degerleri, amoksisilin in 0. IN NaOH iqerisindeki qozeltilerinin 220-350 nm araligindaki UV spektrumlannda 247.0 nm de dlqulmustiir. Birinci turevUV spektrofotometride, dA/dX degerleri, amoksisilin in 0. IN NaOH iqerisindeki qozeltilerinin 220-320 nm araligindaki birinci turev UV spektrumlannda (AX= 2 nm) 255.8 nm de dlqulmustiir. Ikinci turev UV spektrofotometride ^A/dX 2 degerleri amoksisilin in 0. IN NaOH iqerisindeki qozeltilerinin 220-320 nm araligindaki ikinci turev UV spektrumlannda (AX= 4 nm) 255.8 nm de dlqulmustiir. (Qalismada dogrusal qalisma araligi her uq yontem iqin de 3.2 -48.0 /ug/mL olarak bulunmustur. Yontemlerdeki ortalama geri kazanim ve bagil standart sapma degerleri sirasiyla orijinal UV spektrofotometride 247.0 nm de % 99.67 ve % 1.20, birinci turev UV spektrofotometride,255.8 nm de % 99.04 ve % 1.74 ve, ikinci turev UV spektrofotometride249.2 nm de % 99.43 ve % 2.35 olarak bulunmustur. Gelistirilen uq yontem Turkiye ilaq piyasasinda bulunan 8 adet tablet, 2 adet oral suspansiyon ve bir adet liyofilize toz formulasyonuna basariyla uygulanmistir. Elde edilen turn sonuqlar USP XXII de belirtilen yontemlerle elde edilenlerle istatistiksel olarak karsilastinlmistir.
Physical Chemistry, 2012
This paper describes the validation of an innovative analytical method for ampicillin sodium in powder for injection quantification, using Fourier-transform infrared (FT-IR) transmission spectroscopy. This technique does not use organic solvents, which is one great advantage over the most common analytical methods. This fact contributes to minimize the generation of organic solvent waste by the industry and thereby reduces the impact of its activities on the environment. The method involved absorbance measurements of the band corresponding to one of the carbonyl groups in the molecule, centered in the region between 1800 and 1700 cm-1. The method was validated according to ICH guidelines, showing to be linear (r = 0.9993), precise, accurate and robust, over a concentration range from 1.0 to 3.0 mg. The validated method is able to quantify ampicillin sodium in powder for injection preparation and can be used as an environmentally friendly alternative for the routine analysis in quality control.