Phytochemical profile and antibacterial activity of crude extracts of the pod of Aframomum angustifolium (Sonn.) K. Schum (original) (raw)
Related papers
2019
This study was conducted to analyze the chemical constituent and to evaluate the antimicrobial properties of the leaf essential oil of Aframomum latifolium. The leaves of this plant was subjected for hydro distillation using all glass Clevenger apparatus. The volatile oil obtained was analysed by means of gas chromatography mass spectrometry GC Ms . The oil yield was 0.08 v w. Seventeen components were identified in the leaf essential oil accounting for 99.90 of the total oil fraction. The leaf oil was dominated by oxygenated monoterpenes 51.26 and monoterpenes 36.22 . Cajeputol, an oxygenated monoterpene is the component with the highest percentage composition 45.05 , cyclofenchene 7.82 . Peperitone is the component with the lowest percentage 0.43 . The oil displayed high antimicrobial Potentials to Staphylococos aureus, Escherichia Coli, Bacillus subtilis, Pseudomonas aeruginosa, Candida albican and aspergillus niger at 100 , 50 and 25 and a moderate inhibition at 12.5 and 6.25 Th...
Phytochemical characterization and the antimicrobial property of Aframomum danielli extract
Characterization of preliminary phytochemical components of Aframomum danielli seeds was determined. Fractions of the seeds obtained by vacuum liquid chromatographic process were tested for antimicrobial activities. Phytochemical screening revealed the presence of alkaloids, cardenolides, carotenoids and polyphenols. All fractions obtained from the petroleum ether extract exhibited antimicrobial activity on food-borne pathogens with minimum inhibitory concentrations in the range of 100 – 800 microgram per millilitres.
The Scientific World Journal, 2020
e antibacterial activity of the extracts of Aframomum melegueta including n-hexane extract (NHE), nondefatted methanol extract (NDME), and defatted methanol extract (DME) was investigated in this study. e NHE exhibited no antibacterial activity. e DME showed higher antibacterial activity than the NDME against the different isolates. At the highest concentration of 10 mg/mL in agar diffusion, NDME produced inhibition zones ranging from 11 to 29 mm against the microorganisms while DME produced inhibition zones ranging from 20 to 40 mm with the concentration of 10 mg/mL against the microorganisms. 0.1 mg/mL of the DME produced inhibition zones ranging between 12 and 14 mm in Aeromonas hydrophila ATCC 35654 and Pseudomonas aeruginosa ATCC 15442, respectively, while none of the isolates were inhibited by the NDME at a concentration of 1 mg/mL or less. In the agar dilution assay, the MICs of the NDME and DME ranged between 0.31 and 10 mg/mL, but more isolates were inhibited at 0.31 mg/mL of DME than those in NDME. In macrobroth assay, the MICs of the NDME ranged between 0.15 and 5.0 mg/mL and the MBCs ranged between 0.63 and 5.0 mg/mL, and the MICs of the DME ranged between 0.08 and 5.0 mg/mL and the MBCs were between 0.31 and 5.0 mg/mL. is study indicated that DME was more active with higher antibacterial activity than the NDME of this plant, and extracting the fatty portion of plant materials prior susceptibility testing would allow plant extracts to be more effective as well as justifying the use of Aframomum melegueta in traditional medicine for the treatment of bacterial infections.
This work has studied the chemical composition of essential oils of limb, leaf sheath and rhizome of Aframomum sceptrum (Oliv. & T. Hand.) K. Schum (Zingiberaceae) collected in southern Benin, by GC and GC / MS and were tested for their effectiveness against pathogenic microorganisms. The main compounds noted in the essential oils of Aframomum sceptrum also called Aframomum masuianum (Oliv & D. Hand) are: for limb: β-caryophyllene (33.4%), β-pinene (28.4%), α-humulene (10.6%), α-pinene (3.3%) and caryophyllene oxide (3.0%), for leaf sheath β-pinene (42.4%) and α-pinene (5,7%) and concerning the rhizomes; the results showed the following: β-pinene (15.9%), α-terpineol (l5.2%), β-caryophyllene (13.9%) and α-humulene (6.4%). The microbiological tests showed that essential oils of three organs of A. sceptrum have no activities related to the microorganisms studied.
ANTIBACTERIAL PROPERTIES OF CRUDE POD EXTRACT OF ACACIA NILOTICA (FABACEAE
Antibacterial activity of different concentrations of Acacia nilotica pod crude extract (200, 400, 600, 800 and 1000 mg/ml of water) on Staphylococcus aureus, Streptococcus pyogenes, Bacillus subtilis, Corynebacterium pyogenes, Klebsiella pneumoniae, Salmonella typhi and Candida albicans was determined. Minimum inhibitory concentration of A. nilotica pod crude extract was 12.5 mg/ml for Bacillus subtilis, C. pyogenes and K. pneumoniae, while it was 25 mg/ml for S. pyogenes and C. albicans and 200 mg/ml for S. aureus Minimum bactericidal concentration of Acacia nilotica pod crude extract was 25 mg/ml for B. subtilis and K. pneumoniae, 100 mg/ml for S. pyogenes and 200 mg/ ml for S. aureus and C. pyogenes. This study revealed the importance of A. nilotica pod as an antibacterial agent and its applications in the treatment of bacterial diseases.
AFRICAN JOURNAL OF BIOTECHNOLOGY
Afzelia africana is used in folklore remedies for the treatment of diarrhoea, gastrointestinal disorders and gonorrhoea among other ailments; hence we assessed the in vitro antimicrobial activities of this important medicinal plant. Thirty bacterial isolates as well as four fungal isolates were tested in this study. The crude extract of the stem bark of the plant exhibited antimicrobial activities at a concentration of 25 mg/ml against twenty-one of the bacterial isolates, (i.e. 72.41% of the tested isolates) comprising both Gram positive and Gram negative strains. The zones of inhibition exhibited by the extract against the test bacterial species ranged between 13 and 22 mm. The Minimum Inhibitory Concentrations (MIC) of the extract vary between 1.56 and 12.50 mg/ml while the Minimum Bactericidal Concentration (MBC) ranged between 3.13 and 25.00 mg/ml. However, the extract lacked activity against all four tested fungal species. Phytochemical assay revealed the presence of alkaloids, tannins, flavonoids and saponins in the extract. We conclude that the stem bark of A. africana is a promising candidate as source of new antibacterial compounds.
Central African Journal of Public Health
The antibacterial and antimycotic activity of Aframomum melegueta seeds were investigated against Escherichia coli, Staphylococcus aureus, Pseudomonas earuginosa, Salmonella species, Klebsiella species, Bacillus species, Fusarium species, Rhizopus species, Aspergillus species, Penicillium species and Mucor species isolated from spoiled bread and tomatoes using agar well diffusion method. The result showed that the ethanol extract exhibited higher antibacterial activity more than the aqueous extract with Bacillus sp. having the highest zone of inhibition (28mm, 23mm), followed by Salmonella sp. (26mm, 22mm), S. aureus (24mm, 19mm), Klesiella sp. (22mm, 17mm) and E. coli (20mm, 16mm) while P. earuginosa was the least (18mm, 15mm). The antifungal activity showed that Rhizopus sp. was the most inhibited by both ethanol and aqueous extracts respectively (20mm and 16mm), followed Penicillium sp. (17mm and 12mm), Aspergillus sp. (14mm and 11mm) and Fusarium sp. (14mm and 10mm) while Mucor sp. was the least (15mm and 9mm). The minimum inhibitory concentrations (MICs) of the aqueous seed extracts showed that MIC of E. coli, S. aureus, Salmonella sp. and Bacillus sp. was 20mg/mL. MIC for P. earuginosa, Klesiella sp. and Rhizopus sp. was 30mg/mL while Fusarium sp., Aspergillus sp., Penicillium sp. and Mucor sp. have MICs of 50mg/mL. The MICs of the ethanolic extract showed that E. coli and S. aureus have MICs of 10mg/mL, P. earuginosa, Klesiella sp., Penicillium sp. and Rhizopus sp. have 20mg/mL, Fusarium sp., Aspergillus sp. and Mucor sp. have 30mg/mL while Bacillus sp. was the most susceptible with MIC of 5mg/mL. The low MICs are indication of strong antibacterial and antimycotic effects of the extracts. Hence, the extracts could be used in treating infections associated with the test organisms and as well as serve as potential food preservative.
ANTI-BACTERIAL EFFECTS OF AFRAMOMUM MELEGUETA SEED EXTRACTS ON SOME ENTEROBACTERIACEAE ORGANISMS
The anti-bacterial effects of the methanol, ethanol and aqueous extracts of A. melegueta seeds were investigated on some enterobacteriaceae organisms (Escherichia coli, Klebsiella pneumoniae, Salmonella tyhi and Shigella spp). The organisms tested showed varying degrees of sensitivity to the various extracts with the aqueous extract having the least activity when compared to the ethanol and methanol extracts which had closely inhibitory effects. The ethanol extract had higher zones of inhibition on the organisms than the methanol extract in the decreasing order: E. coli (21.5 mm), Shigella spp (17.0 mm), S. tyhi (11.5 mm) and K. pneumoniae (9.0 mm). The zones of inhibition exerted on the organisms by the methanol extract were in the decreasing order: S. tyhi (15.0 mm), E. coli (13.5 mm), Shigella spp (12.0 mm) and K. pneumoniae (0.0 mm). This study shows that the seeds of A. melegueta possess active ingredients that may be exploited for the development of anti-microbials and alternative remedies for infections and diseases caused by the tested organisms.
Journal of Experimental Pharmacology
The emergence and spread of resistant microbes continue to be a major public health concern. Effective treatment alternatives, particularly from traditionally used medicinal plants, are needed. Objective: The main objective of this study was to conduct phytochemical screening and antimicrobial activity evaluation of selected traditionally used medicinal plants in Ethiopia. Methods: The ethnomedicinal use value frequency index (FI) was used to select twelve medicinal plants. Phytochemical classes of compounds were screened using different standard methods. Anti-microbial activities of plant extracts were evaluated against Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, and Candida albicans. Minimum inhibitory concentrations were measured using the broth micro-dilution method. The data were analyzed using Statistical Package for the Social Sciences (SPSS) version 21.0 and the findings were presented descriptively and using non parametric one-way ANOVA analysis (Kruskal-Wallis/Ddunn's test). Results: The phytochemical constituents identified were flavonoids, alkaloids, glycosides, phenols, saponins, steroids, and terpenoids, with flavonoids, alkaloids, and phenols being the most abundant. The crude extracts and chloroform fractions of the extracts showed an activity against the tested strains. The crude extract of Thalictrum rhynchocarpum Quart.-Dill. and A.Rich root demonstrated superior activity against all the tested strains with the lowest minimum inhibitory concentrations of 0.48 μg/mL against Staphylococcus aureus and Escherichia coli; 0.98 μg/mL against Klebsiella pneumoniae, Pseudomonas aeruginosa; and 3.90 μg/mL against Candida albicans, which are even better than the reference drug, gentamicin and clotrimazole. Conclusion: The majority of evaluated medicinal plants demonstrated remarkable activity against tested microbial strains, which can be attributed to the presence of secondary metabolites of different classes of compounds. The finding provided scientific evidence for the use of these traditionally used medicinal plants.
This work has studied the chemical composition of essential oils of limb, leaf sheath and rhizome of Aframomum sceptrum (Oliv. & T. Hand.) K. Schum (Zingiberaceae) collected in southern Benin, by GC and GC / MS and were tested for their effectiveness against pathogenic microorganisms. The main compounds noted in the essential oils of Aframomum sceptrum also called Aframomum masuianum (Oliv & D. Hand) are: for limb: β-caryophyllene (33.4%), β-pinene (28.4%), α-humulene (10.6% ), α-pinene (3.3%) and caryophyllene oxide (3.0%), for leaf sheath β-pinene (42.4%) and α-pinene (5,7%) and concerning the rhizomes; the results showed the following: β-pinene (15.9%), α-terpineol (l5.2%), β-caryophyllene (13.9%) and α-humulene (6.4%). The microbiological tests showed that essential oils of three organs of A. sceptrum have no activities related to the microorganisms studied.