SuperSelective primer pairs for sensitive detection of rare somatic mutations (original) (raw)
Selective Amplification of Rare Mutations Using Locked Nucleic Acid Oligonucleotides that Competitively Inhibit Primer Binding to Wild-Type DNA
Reid Oldenburg
Journal of Investigative Dermatology, 2008
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Ligation of a primer at a mutation: a method to detect low level mutations in DNA
Brendan Price
Mutagenesis, 2002
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Detection of point mutations with a modified ligase chain reaction (Gap-LCR)
John Carino
Nucleic Acids Research, 1995
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Single-molecule PCR: an artifact-free PCR approach for the analysis of somatic mutations
Konstantin Khrapko
Expert Review of Molecular Diagnostics, 2005
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Highly selective Cooperative Primers for multiplex detection of rare mutations
Masen Christensen
bioRxiv (Cold Spring Harbor Laboratory), 2021
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Mutant DNA Polymerase for Improved Detection of Single-Nucleotide Variations in Microarrayed Primer Extension
Ramon Kranaster
ChemBioChem, 2008
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Arrayed Primer Extension: Solid-Phase Four-Color DNA Resequencing and Mutation Detection Technology
Neeme Tõnisson
Genetic Testing, 2000
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Single base pair mutation analysis by PNA directed PCR clamping
Henrik Orum
Nucleic Acids Research, 1993
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A novel one cycle allele specific primer extension—Molecular beacon displacement method for DNA point mutation detection with improved specificity
YUAN LI
2007
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High specificity primers, amplification methods and kits
Fred Kramer
2002
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Mode of action and application of Scorpion primers to mutation detection
Tom Brown
Nucleic Acids Research, 2000
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Kinetic Hairpin Oligonucleotide Blockers for Selective Amplification of Rare Mutations
Yanwei Jia
Scientific Reports, 2014
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Mutation Detection by Denaturing DNA Chromatography Using Fluorescently Labeled Polymerase Chain Reaction Products
Karl Hecker
Analytical Biochemistry, 1999
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A one-tube, two-step polymerase chain reaction-based site-directed mutagenesis method with simple identification of the mutated product
László Puskás
Analytical Biochemistry, 2004
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Mutation detection by primer extension on oligonucleotide microarrays
Neeme Tõnisson
2002
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Analysis of somatic mutations via long-distance single molecule PCR
Konstantin Khrapko
DNA …, 2004
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Improvement of fragment and primer selection for mutation detection by denaturing gradient gel electrophoresis
Vanessa Hayes
Nucleic Acids Research, 1998
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Multiplex Real-Time PCR Assays that Measure the Abundance of Extremely Rare Mutations Associated with Cancer
Fred Kramer
PloS one, 2016
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Detection of human genomic mutations by chemical single-reaction DNA sequencing
Giovanna Costanzo
Technical Tips Online, 1997
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Multiplex detection of mutations
David Perlin
Methods in molecular biology (Clifton, N.J.), 2008
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Primer-template misalignments during leading strand DNA synthesis account for the most frequent spontaneous mutations in a quasipalindromic region in Escherichia coli
lynn ripley
Journal of Molecular Biology, 1998
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Bipolar clamping improves the sensitivity of mutation detection by temperature gradient gel electrophoresis
Patrick Booms
Electrophoresis, 1998
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Detection of single-base DNA mutations by enzyme-amplified electronic transduction
Itamar Willner
Nature biotechnology, 2001
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Multiplex SuperSelective PCR Assays for the Detection and Quantitation of Rare Somatic Mutations in Liquid Biopsies
Fred Kramer
The Journal of Molecular Diagnostics, 2021
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Simplified one-tube “megaprimer” polymerase chain reaction mutagenesis
Ross Nazar
Analytical Biochemistry, 2005
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5′-Degenerate 3′-Dideoxy-Terminated Competitors of PCR Primers Increase Specificity of Amplification
Sergei Atamas
BioTechniques, 1998
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The implications of using mutagenic primers in combination with Taq polymerase having proofreading activity
Angeliki Hatzaki
Biologicals, 2004
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Sequence-specific "gene signatures" can beobtained by PCR with single specific primers at low stringency
Gunawan Wibowo
Proceedings of the National Academy of Sciences, 1994
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The elimination of primer-dimer accumulation in PCR
David Whitcombe
Nucleic Acids Research, 1997
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Absolute Quantification of the Alleles in Somatic Point Mutations by Bioluminometric Methods based on Competitive Polymerase Chain Reaction in the Presence of a Locked Nucleic Acid Blocker or an Allele-Specific Primer
Alexandra Iliadi
Analytical Chemistry, 2011
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Degradable dUMP Outer Primers in Merged Tandem (M/T)-Nested PCR: Low- and Single-Copy DNA Target Amplification
Robert Gore-Langton
Analytical Biochemistry, 1998
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Directed-Mutagenesis and Deletion Generated through an Improved Overlapping-Extension PCR Based Procedure
Frank Robb
Silpakorn U Science & …, 2007
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Improved heteroduplex detection of single-base substitutions in PCR-amplified DNA
Armand Peeters
Genome Research, 1994
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