Effect of lipid emulsions on production and fatty acid composition of eggs of the scallop Argopecten purpuratus (original) (raw)
Related papers
Aquaculture, 2003
The impact of dietary fatty acids on the fatty acid composition and content in different organs (digestive gland, adductor muscle and male and female gonad) of Argopecten purpuratus was evaluated during the process of gonad maturation under controlled laboratory conditions. A mixed algal diet of Isochrysis galbana (clone T-Iso), Pavlova lutheri, Chaetoceros gracilis and Tetraselmis suecica was either fed alone (Al) or supplemented with 50% lipids (expressed as % of the algal dry weight (DW)) using an emulsion rich in 22:6n À 3 (Al + EmDHA) or an emulsion rich in 20:5n À 3 (Al + EmEPA). Results showed the algal diet having the greatest impact on the fatty acid composition (mainly an increase of 18:1n À 9, 18:2n À 6; 18:3n À 3 and a decrease of 20:5n À 3) of the digestive gland and female gonad, moderate in the male gonad while the fatty acid composition of the adductor muscle was hardly affected. Our data indicated that 16:1n À 7 and 18:1n À 9 were partially elongated to 18:1n À 7 and 20:1n À 9, respectively. The supplementation of the algal diet with EmDHA or EmEPA (which contained 45% and 19% 22:6n À 3, respectively) increased significantly the concentration of 22:6n À 3 (mg g À 1 DW) in the digestive gland, female gonad and male gonad with 78%, 57% and 51%, respectively, for (Al + EmDHA)-fed scallops and 18%, 33% and 19%, respectively, for (Al + EmEPA)-fed scallops, while the 22:6n À 3 concentration in the adductor muscle was not affected. Broodstock conditioning was associated with a significant increase of the lipid content in the female and to a lesser extent in the male gonad. The significant increase (as compared to the initial sample) of the 22:6n À 3 concentration in the digestive gland, 0044-8486/03/$ -see front matter D Aquaculture 217 female gonad and eggs of A. purpuratus fed 22:6n À 3-rich lipid illustrated that recently ingested fatty acids did significantly contribute to the lipid accumulation process in the maturing female gonad. The similarities between the fatty acid composition of the female gonad and digestive gland suggest that lipids were transferred from the lipid-rich digestive gland to the female gonad. D
Journal of Plankton Research, 2015
In cyclical parthenogenetic Daphnia, asexual eggs develop immediately and enable fast population growth, while sexual eggs are dormant and can survive harmful conditions. We studied whether this different function is reflected in different fatty acid profiles and explored the capacity of D. magna to adjust fatty acid provisioning of its eggs depending on food resources. We quantified neutral-and phospholipid content of sexual and asexual eggs produced under different food conditions and compared these with eggs collected from a natural pond. In eggs obtained under different laboratory food regimes, total concentration of neutral fatty acids per unit biomass was not affected by food source or egg type. Both egg types contained lower amounts of fatty acids in the neutral fraction when produced in nature than under laboratory conditions. Fatty acid concentration in the phospholipid fraction was lower in sexual than asexual eggs. Fatty acid composition of eggs largely reflected that of the food of the mothers, albeit with small modifications. Sexual eggs produced on a diet of Scenedesmus obliquus (no C20-PUFA), contained higher concentrations of eicosapentaenoic acid and arachidonic acid in both fractions than asexual eggs.
Aquaculture Research, 2011
The present study examines the total lipid (TL) and fatty acid (FA) composition in eggs of brill Scophthalmus rhombus L. and the possible relationships with their quality parameters. Wild broodstocks were caught and maintained in captivity until eggs were collected. A lipid characterization of each egg batch was conducted in TL, lipid classes (LC) and FA of TL. The TL content was lower than the values reported for other £at¢sh species, showing high levels of sterol esters (SE). High viability rates were related to higher lipid reserves. Higher cholesterol (CHO) was linked to higher egg viability, whereas SE could have an opposite e¡ect. Comparison of female quality showed that lipid composition was more related to egg batches than to individual females. However, multivariate analysis did not show a clear correlation between lipid composition and brill egg quality, neither as individual components nor as a whole pro¢le of LC or FA. Our results suggest that some lipid components (phosphatidylcholine, CHO, SE, monounsaturated, eicosapentaenoic acid) could be related to di¡erences in spawning quality, although these were not the only factors involved in these di¡erences. Thus, these lipid components could be considered to be descriptors of the di¡erences found in the rates of brill quality.
Aquaculture, 1997
Samples of sea bass (Dicetztrarchus labraxi eggs from broodstock which had been fed either a formulated pelleted feed, containing fish and corn oil, or a local trash fish, bogue (Boops boopsl were analysed for lipid class compositions, fatty acid compositions of phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylinositol (PI) and wax ester and fatty alcohol compositions of wax esters. The pelleted feed contained 1.3 mg g -' of arachidonic acid (20:4n-6; AA) and an AA/eicosapentaenoic acid (20:5n-3; EPA) ratio of 0.1 while the trash fish contained 4.8 mg g-' AA and an AA/EPA ratio of 0.7. Docosahexaenoic acid (22:6n-3; DHA) concentrations were similar for both diets (about 23 mg g-' ). The fatty acid compositions of PC, PE and PI from eggs of fish fed trash fish contained significantly more AA. 22:5n-6 and DHA compared to fish fed the pelleted feed. The AA/EPA ratios in these phospholipids were around five-fold higher in the trash fish-fed group compared to those fed the fish and corn oil containing diet. In PI, which contains characteristically high levels of AA, the AA/EPA ratios were 1.5 and 8.6 for eggs derived from broodstock fed the pelleted diet and the trash fish, respectively. Determination of lipid class compositions of sea bass eggs revealed the presence of high levels of wax esters which were previously unrecorded in this species. The use of broodstock diets containing blends
Fish Physiology and Biochemistry, 1998
A feeding experiment was conducted on gilthead seabream (Sparus aurata) broodstock to investigate the incidence of n-3 highly unsaturated fatty acids (n-3 HUFA) dietary deficiencies on the lipid composition of female liver, gonads and eggs, in relation to spawning quality. Broodstock were fed a control (C) diet or a n-3 HUFA deficient (D) but linolenic acid rich diet. After 20 weeks of feeding, the results showed that levels of total neutral (TNL) and total polar (TPL) lipids of female gonads and eggs were independent of diet. However the fatty acid composition of phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylinositol (PI) of female liver, gonads and eggs in the two groups of fish showed marked differences, reflecting the influence of fatty acid levels in the broodstock diets. This influence was even higher in TNL than in the phospholipid classes examined. In fish fed n-3 HUFA deficient diet, fatty acid composition of TNL of female gonads and eggs reflected the diet more than liver. A higher egg production in broodstock fed C diet (1.8% n-3 HUFA in diet) was extended to spawning quality such as percentages of fertilised and hatched eggs.
Scientia Marina, 2001
To determine whether an increase in the quantity of lipids ingested by sea bass (Dicentrarchus labrax) broodstock could improve egg quality, three year-old sea bass were fed three different diets: a natural diet (Control group), and two artificial diets containing 10% or 17% of total lipids. In two consecutive reproductive seasons, the spawning results, the lipid classes and the fatty acid composition of the eggs were studied. No differences in the absolute content of lipids or in the percentage of different lipid classes were observed between the eggs from the three groups. Both experimental groups fed with the artificial diets produced eggs of poor quality, with low percentages of buoyancy and hatching. Those observed in the eggs from the Control Group were significantly higher (p>0.01) than those of the experimental groups. The higher hatching rate of the eggs from the Control Group was associated with higher DHA:EPA and AA:EPA ratios. The data obtained showed that the fatty acid composition of the eggs was affected by the fatty acid composition of the diets but not by the total quantity of lipids administered to the broodstock.
Latin American Journal of Aquatic Research
The sexual maturation and reproduction of crustaceans are very demanding processes for energy and nutrients. Females require the nutrients to deposit them into the eggs and allow the healthy development of embryos and early larvae survival. The lipids are essential nutrients related to the sexual maturation and the egg production, as they are sources of metabolic energy for the gonad development, as well structural molecules for the embryonic tissue formation. This work presents the effects of different dietary lipid levels (10, 12.5, 15, 17.5 and 20% of a mixture of krill and cod liver oil as lipid sources), on the growth, egg production, and egg quality, of freshwater prawn Macrobrachium acanthurus females fed during 60 days. The results showed that an inclusion higher than 15% of lipids, improved the egg production and the content of protein and lipids significantly. A lipid inclusion between 15 and 17.5% might be optimal for maturation and egg production of the females of M. acanthurus.
Aquaculture Research, 2009
The e¡ects of dietary n-3 highly unsaturated fatty acid (n-3 HUFA) on eggs and larval quality were investigated in the Chilean £ounder Paralichthys adspersus. Broodstock were fed with three formulated diets with similar proximate compositions but di¡erent n-3 HUFA (2.1%, 3.1% or 4.1%) estimated levels from 5 months before and during the spawning period. The diet with an intermediate n-3 HUFA level resulted in a signi¢cantly higher (Po0.05) percentage of buoyant eggs (68.2 AE 2.9%), fertilization (92.8 AE 3.9%), normal cell cleavages (93.5 AE 1.9%), hatching rate (87.7 AE 4.1%) and normal larvae (76.3 AE 3.7%) compared with the other two diets. In contrast, high levels of n-3 HUFA produced larvae with a higher survival capacity when subjected to fasting. The diet with the lowest content of n-3 HUFA produces lower quality eggs and larvae. The n-3 HUFA level in eggs increased with an increase in the dietary level, and the n-3/n-6 ratios were1:1, 2:1and 3:1. The DHA/EPA and EPA/ARA ratios of 2 and 4 in eggs, respectively, were associated with improved egg and larval quality and were similar to the ratios found in eggs from wild broodstock. Attainment of optimal fatty acid contents in broodstock diets is one of the key factors for producing the high-quality spawning required for managed culture of this £ounder.
Scientia …, 2001
In particular, the lipidic fraction of the diets has been shown to exert a clear influence on egg viability. Studies carried out on rainbow trout, Oncorhynchus mykiss (Watanabe et al., 1984), red sea bream, Pagrus major (Watanabe et al., 1991), and gilthead sea bream, Sparus aurata (Zohar et al., 1995), demonstrated that ovarian and embryonic development were greatly affected by the fatty acid composition of the diet. All these experiments showed that it was necessary to provide an adequate quantity of n-3 fatty acids to prevent low hatching rates and poor larval survival. Some n-3 fatty acids, the highly unsaturated fatty acids (HUFA) eicosapentaenoic acid (EPA, 20:5 n-3) and docosa
Aquaculture, 2007
This study was conducted to determine the optimum phospholipids: highly unsaturated fatty acids (PL/HUFA) ratio in maturation diets for female Chinese mitten crab, Eriocheir sinensis broodstock. Five isolipidtic and isonitrogenous diets were formulated to contain different amounts of pork lard, fish oil and soy lecithin with resulting PL/HUFA levels (%dry weight) of 1.4/0.3 (Diet 1), 1.6/2.5 (Diet 2), 2.3/2.0 (Diet 3), 3.8/1.5 (Diet 4) and 5.1/0.8 (Diet 5). Diet 1 lacked supplemental fish oil and soy lecithin and served as the control. Each of the five formulated diets was fed to a total of 150 female crabs for a period of 7 months. All diet treatments were triplicated with 50 crabs stocked in a pond as a replicate. After 6 months, male crabs were introduced into the ponds where females were kept, mating, spawning and egg hatching (in seawater) occurred in the following month. The nutritional value of various formulated diets was assessed based on survival, gonadosomatic index (GSI), egg production per female, fecundity (eggs/g female weight) of the females fed those diets and egg and larval (newly hatched zoea I larvae) quality.