Oxidative Stress Biomarkers as Prognostic Indicators of Severity in Patients With Dengue (original) (raw)
Related papers
Journal of Tropical Life Science
The objectives of this study were to determine the involvement of Oxidative Stress (OS) in the pathogenesis of dengue hemorrhagic fever (DHF) through the analysis of oxidative stress Index (OSI). The levels of malondialdehyde (MDA), superoxide dismutase (SOD) and catalase (CAT) activity, and OSI were measured in 61 child dengue patients and (aged 6 months-18 years) with three different stages of DHF, i.e stage I, II, and III. The results show that the levels of MDA, SOD and CAT activity, and OSI significantly different between the group. The all parameters that investigated in this present study seems higher MDA level and OSI in the higher grade of DHF, except for SOD and CAT activity. From this result, it can be concluded that oxidative stress pathways might be involved in the pathomechanism of DHF and OSI might be used as a biomarker for OS and the severity in DHF patients.
Oxidative stress in adult dengue patients
The American journal of tropical medicine and hygiene, 2004
An association between viral diseases and increased oxidative stress has been suggested. The time course of serum levels of total antioxidant status (TAS), peroxidation potential (PP), glutathione (GSH), lipid peroxidation measured as hydroperoxides, and malondyaldehyde and 4-hydroxyalkenals (MDA + 4-HDA), as well as antioxidant enzymatic activity of superoxide dismutase (SOD) and glutathione peroxidase (GPx), were measured in 22 serologically confirmed dengue patients. Most of the patients had dengue fever and three of them had dengue hemorrhagic fever. The redox parameters were compared with those of age- and sex- matched controls. No significant difference was observed for levels of GSH and TAS between patients and controls. Levels of PP, MDA + 4-HDA, and SOD were significantly higher. Levels of GPx and total hydroperoxides were significantly lower in patients in comparison with controls. These findings suggest that the alteration in redox status could result of increased oxidati...
Acta Tropica, 2008
Objectives: Oxidative stress in dengue viral infection has been suggested and severity of it was found to be associated with progress of illness. Hence assessing oxidative stress mediated changes in plasma proteins can be an early biomarker for prediction of severe dengue infection. Design and methods: Thirty two dengue hemorrhagic fever (DHF), 21 dengue shock syndrome (DSS), 27 dengue fever (DF) and 63 age and sex matched controls, were included in this study. Blood samples were collected on the 3rd day of fever. Protein carbonylation (PCOs) and protein-bound sulphydryl (PBSH) group levels were determined by spectrophotometric method and analyzed as predictor of dengue hemorrhagic fever and dengue shock syndrome. Results: About 80-84% of cases presented with no signs of DHF/DSS at the time of sampling. Dengue infected individuals had significantly elevated PCOs and low PBSH group levels than the controls. Using one-way ANOVA we found a significant difference with high PCOs and low PBSH group levels between DHF and DSS when compared with DF (P < 0.001). However, no difference was observed in PBSH group levels between DHF and DSS. A significant difference in PCOs to PBSH ratio was observed among DF, DHF and DSS (P < 0.001). Linear regression analysis revealed that duration of hospitalization is dependent on PCOs and PBSH group levels. Receiver operator curve (ROC) analysis indicated that 5.22 nmol/mg protein PCOs; 1.08 PCOs to PBSH group levels ratio were optimal cutoff value for predicting DHF with sensitivity and specificity of 87.5% and 74.1%; 96.9% and 81.5%, respectively. For DSS prediction, 6.13 nmol/mg protein PCOs; 1.16 PCOs to PBSH group levels ratio were found as effective cutoff with sensitivity and specificity of 81% and 71.9%; 95.2% and 56.2%, respectively. Conclusion: Oxidative stress has been observed to develop since early days of onset of dengue infection. Plasma PCOs, PCOs to PBSH group ratio were found to very well predict DHF/DSS.
Oxidative damage in dengue fever
Free Radical Biology and Medicine, 2009
Oxidative stress may be important in the pathogenesis of dengue infection. Using accurate markers of oxidative damage, we assessed the extent of oxidative damage in dengue patients. The levels of hydroxyeicosatetraenoic acid products (HETEs), F 2 -isoprostanes (F 2 -IsoPs), and cholesterol oxidation products (COPs) were measured in 28 adult dengue patients and 28 age-matched study controls during the febrile, defervescent, and convalescent stages of infection. We compared the absolute and the percentage change in these markers in relation to key clinical parameters and inflammatory markers. The levels of total HETEs and total HETEs/arachidonate, total F 2 -IsoPs/arachidonate, and COPs/cholesterol were higher during the febrile compared to the convalescent level. Total HETEs correlated positively with admission systolic blood pressure (r = 0.52, p b 0.05), whereas an inverse relationship was found between 7β-hydroxycholesterol and systolic and diastolic blood pressure (r = −0.61 and −0.59, respectively, p b 0.01). The urinary F 2 -IsoP level was higher in urine during the febrile stage compared to the convalescent level. Despite lower total cholesterol levels during the febrile stage compared to convalescent levels, a higher percentage of cholesterol was found as COPs (7β-, 24-, and 27-hydroxycholesterol). The levels of platelet-activating factoracetylhydrolase activity, vascular cellular adhesion molecule-1, tumor necrosis factor-α, and high-sensitivity C-reactive protein were higher during the febrile stage compared to their convalescent levels (p b 0.01). Markers of oxidative damage are altered during the various stages of dengue infection.
ANALYSIS OF ANTIOXIDANTS LEVEL IN DENGUE FEVER
Introduction: Dengue is the most frequent viral infection, with > 100 million infections throughout the world annually, including 250,000-500,000 cases of dengue hemorrhagic fever (DHF) and 24,000 deaths. Objectives of the study: The main objective of the study is to analyse the levels of antioxidants in dengue fever among local population of Pakistan. Material and methods: This cross-sectional study was conducted in Nishtar Hospital, Multan during June 2019 to December 2019. The data was collected from 100 patients of both genders. Antioxidants levels in the blood were measured by getting blood samples of patients. 5cc blood sample was taken and then it centrifuged at 4000rpm for the separation of serum. Results: The data was collected from 100 patients of both male and female patients. SOD activity in the serum and liver was dramatically and significantly decreased after infection when compared to normal values. MDA levels in the serum were significantly elevated when compared to the controls (p<0.05). CAT activity in the liver showed a significant decrease when compared with the controls (p<0.05), whereas there was no obvious change in CAT levels in the serum. Conclusion: It is concluded that dengue viral infection results in a significant alteration in the oxidative status in the liver, as shown by increased ROS production, MDA levels and the GSSG/GSH ratio and decreased levels of SOD and CAT.
International Journal of Infectious Diseases, 2014
Objectives: Proinflammatory cytokines and the oxidative stress response are reported to be involved in dengue viral disease. The present study investigated the correlation of proinflammatory cytokines and lipid peroxidation with dengue severity. Methods: Clinical samples from 27 dengue fever (DF) cases, 30 dengue haemorrhagic fever (DHF) cases, and 24 dengue shock syndrome (DSS) cases were studied around defervescence, along with samples from 30 healthy controls. Plasma samples were analysed for tumour necrosis factor alpha (TNF-a) and interferon gamma (IFN-g) by ELISA and for malondialdehyde (MDA) by thiobarbituric acid assay. Results: Dengue-infected individuals had significantly higher levels of TNF-a, IFN-g, and MDA in comparison to controls. The ratio of TNF-a to IFN-g was significantly higher in DHF and DSS than in DF. A TNF-a/IFN-g ratio value of 5.69 around defervescence predicted DHF and DSS with moderate accuracy and thus may serve as an indicator to study dengue severity. The study observed a significant positive correlation of lipid peroxides with TNF-a levels and the TNF-a/IFN-g ratio in severe dengue cases. Conclusions: We propose that the oxidative stress response induced by the dengue virus may trigger the inflammatory cytokine responses in dengue severity and thereby contributes to the pathogenesis of the disease; however the interplay between the oxidative response and inflammatory activity in disease virulence needs further study.
Oxidative-nitrosative stress and dengue disease: a systematic review of in vivo/in vitro studies
Revista Cubana de Medicina Tropical, 2015
Objective : In this systematic review the aim was to summarise the in vivo / in vitro evidence on the role of oxidative-nitrosative stress in pathogenesis of dengue. Methods : We searched electronic databases (PubMed, EMBASE, The COCHRANE library, ScienceDirect, Scopus, SciELO, LILACS via Virtual Health Library, Google Scholar) using the term: dengue, dengue virus, severe dengue, oxidative stress, nitrosative stress, antioxidants, oxidants, free radicals, oxidized lipid products, lipid peroxides, nitric oxide, and nitric oxide synthase. Articles were selected for review by title and abstract excluding letter, review, epidemiological studies, and duplicates studies. Selected articles were reviewed for used animal model or cell cultures, original purposes, strain of virus or type of antibody, main outcomes, methods, and oxidative-nitrosative stress markers values. Results: In total, 4330 non-duplicates articles were identified from computerized searches of reference databases, of whic...
2021
Background Severe dengue (SD), experienced by only a fraction of dengue patients, can be lethal. Due to the lack of early markers that can predict the evolution of SD, all dengue patients have to be monitored under hospital care. We discovered early oxidative stress markers of SD to identify patients who can benefit from early intervention before the symptoms appear. Methods The expression of inducible nitric oxide synthase (iNOS) in peripheral blood cells (PBC), nitric oxide (NO), and oxidized low-density lipoprotein (oxLDL) levels in plasma and saliva collected at early stages of dengue infection from 20 nonsevere dengue fever (DF) patients and 20 patients who later developed SD were analyzed in a retrospective nested case-control study. Results The expression of iNOS is significantly (P < 0.05) lower in patients who developed SD than in DF patients at admission within 4 days from fever onset. Median plasma NO concentration within 4 days from fever onset is also significantly (...