Stereologic and fine-structural studies of prostatic acinar basal cells in the dog (original) (raw)
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The ultrastructure of basal cells of rat and dog prostate
Cell and Tissue Research, 1976
The ultrastructure of the basal cells of rat lateral and ventral prostate and of dog prostate has been studied. Basal cells from both species appear as undifferentiated cells, characterised by a lack of cytoplasmic organelles and a poorly developed Golgi complex and endoplasmic reticulum. The presence of cytoplasmic filaments and micropinocytosis is not considered to be sufficient evidence to assume any similarity to myoepithelium, as has been previously suggested. Basal cells are instead considered to be precursors of secretory epithelial cells.
HISTOMORPHOLOGY OF PROSTATE GLAND IN DOGS
Indian Journal of Veterinary and Animal Sciences Research, 2021
Prostate gland is the accessory sex gland found in dog. The gland was collected from apparently healthy six sexually mature dogs. The prostate gland was bilobed exocrine gland. Histologically, the prostate gland consisted of stroma and parenchyma. The stroma consisted of capsule, septa and interstitial connective tissue. Parenchyma was enclosed by a thick capsule with outer thin fibrous and inner thick fibromuscular layer. The capsule contained blood vessels, myelinated nerve fibres and nerve ending like meissner’s corpuscle, pacinian corpuscle. Fibromuscular septa entered the parenchyma and divided it into lobes and lobules of different shapes and sizes. Paraenchyma of the prostate gland was consisted of glandular portion and collecting ducts. The gland was compound tubuloalveolar in type. Parenchyma of prostate gland was divided into lobules with many glandular follicles. The glandular follicles showed two zones namely outer and inner zones. The glandular follicles were lined by simple columnar epithelium with oval dark staining nucleus at the basal part and eosinophilic staining part apically. The duct system was ordered into tertiary, secondary and primary ducts. The secretory glandular portion entered into the tertiary duct, to secondary duct and finally to the primary duct. The primary duct then opened into prostratic sinus. The primary, secondary and tertiary collecting ducts and prostratic sinus were lined by simple cuboidal epithelium, simple cuboidal or columnar epithelium and stratified cuboidal epithelium. The prostatic urethra were lined by transitional epithelium. Keywords: Dog, Histology, Prostrate gland
Characterization of canine prostatic cells from normal and hyperplastic glands
Molecular and Cellular Endocrinology, 1980
Secretory and non-secretory epithelial cells and fibroblasts obtained from normal and hyperplastic canine prostate glands and from prostates of 6-week castrated dogs are cultured in monolayers. Prostatic fibroblasts are grown in non-selective culture medium and found at densities of 1.040-1.045 g/ml in Percoll gradients. Enriched populations of each epi~e~~al cell type are obtained by varying the duration of the culture combined with the use of selective MEM D-Val mixture. When separated by centrifugation in Percoll density gradients, the secretory cells (high A.P.) are found at densities of 1.02-l .03 g/ml whereas the non-secretory cells (low A.P.) have densities of 1.05-1.06 g/ml. Both epithelial cell types are present in the normal and hyperplastic glands at the time of explantation. There is no correlation between the prostatic weight and the proportion of each cell type present in the tissue. On the basis of cell density in Percoll gradients and A.P. activity, those prostates with a high percentage of non-sccretory epithelial cells yield better attachment and overa cultures than glands consisting mainly of secretory cells. Our results strongly suggest that non-secretory cells are precursors of the secretory type. In addition, the cells involved in the aging process of the culture are the secretory epithelial cells.
Original Contribution LOCALIZATION AND SHAPE OF BASAL CELLS IN FELINE PROSTATE GLAND
2010
PURPOSE: To study the localization and the shape of basal cell in the prostate gland of the cat with regard to assist the understanding of their role in the pathogenesis of benign and malignant lesions in this animal species. MATERIALS AND METHODS: The prostate glands of 12 sexually mature, clinically healthy male European Shorthair cats at the age of 1–2 years, weighing 2.8 tо 4 kg were investigated. The localization and the shape of basal cells were determined in semi-thin and ultrathin cross sections by light and transmission electron microscopy. RESULTS: Epithelial basal cells in feline prostate alveoli did not attain the alveolar lumen and formed an incomplete, discrete boundary layer, located in close vicinity to the basal membrane. These cells are observed as an occasional and rare epithelial population in the alveolar part of feline prostate parenchyma. The shape of alveolar basal cells varied from oval and triangular to irregular. Basal cells were also observed in the epith...
Differentiation, 1996
Wholemount immunocytochemical staining was used to visualize basal and luminal epithelial-cellspecific cytokeratin and smooth muscle α-actin expression in the developing and adult rat prostate, in the pregnant rat mammary gland and adult rat salivary gland. The stained samples were examined using an Edge R400 3D microscope. Images were collected in both single-image and stereo-pair formats. Prostatic basal epithelial cells were found to have a cell body covering an area of 52-62 µm 2 . The mean footprint size of basal cells was not significantly different between prostatic lobes. Basal epithelial cells were most dense in the anterior and most sparse in the ventral prostatic lobes. Basal epithelial cells had a large body with many processes, which spread around the duct and projected between luminal cells towards the lumen. These processes closely approached their counterparts from adjacent basal cells. In the developing prostate the differentiation of the basal cells from undifferentiated epithelial cords was observed at the region of ductal widening associated with canalization. Following castration prostatic basal epithelial cells became more closely packed, though the size of individual cells was not significantly changed. There was a two-to four-fold increase in basal cell density by 7 days after surgery. Most prostatic luminal cells were found to have hexagonal bases though some were pentagonal in shape. Luminal cells had a mean basal area of 50 µm 2. In the prostate immunocytochemical staining against smooth muscle α-actin revealed discrete bands of muscle surrounding individual prostatic ducts. In the mammary and salivary glands the epithelium was organized into an alveolar arrangement. In the salivary gland a single basal epithelial cell covered the top of each alveolus with processes arranged down the side of the structure. In the mammary gland several basal cells were draped over each alveolus. The mammary and salivary gland basal cells expressed smooth muscle α-actin, indicating their myoepithelial phenotype. The organization of the mam-mary and salivary gland basal cells placed them in an ideal position to squeeze the alveolar structures.& b d y :
Distinct vascular zones in the canine prostate
Microscopy Research and Technique, 2000
The vascular bed of canine prostate was studied and detailed distinct vascular zones were visualized on corrosion casts by scanning electron microscopy. This study was performed because of scarce information about the zonal vascularization of the prostate gland in dogs. There are no studies for three-dimensional microvascular distribution of the capsular vessels and the capsular microvascular trabeculae. SEM (vascular corrosion casting method) was used to show 3D angioarchitecture of the prostate gland. The lobules on the dorsal and lateral surface of the gland were numerous but small. Their small size is probably due to the abundant blood supply in the region. Few but large ventral lobules were observed. Three prostatic zones were clearly defined: capsular, parenchyma, and urethral. The diameter of the venous blood vessel compared to arterial vessels of the capsule was smaller. Two types of arteries were observed in trabeculae: direct and branched. The direct arteries were straight, with only a few branches. The branched arteries contained many bifurcations, with the vessel's diameter decreasing gradually. The trabeculae capillary network formed loops, with frequent sphincter-like constrictions and pouch-like protuberances.
Research in veterinary science, 2014
The purpose of this retrospective study is to evaluate the frequency and further characterize the pathological features of common and uncommon histological lesions in 111 canine prostatic samples. Benign prostatic hyperplasia, suppurative and non-suppurative prostatitis, and prostate cancer were observed individually or in combination in 45, 11, 68 and 50 samples, respectively. Six growth patterns of prostatic carcinoma were differentiated: papillary, cribriform, solid, small acinar/ductal, signet ring, mucinous. In a few cases, perineurial invasion and collagenous micronodules were observed. Lesions considered preneoplastic in men, such as high-grade prostatic intraepithelial neoplasia (HGPIN) and prostatic inflammatory atrophy (PIA), were observed in 27 and 21 histological samples, respectively. This study represents a detailed characterization of the different histological subtypes of canine prostate cancer. The awareness of the unusual patterns might be critical in avoiding diag...