Double Haploid Technique : In Soybean and Other Species (original) (raw)
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Review on Concept and Impact of Double Haploid Techniques in Crop Improvement
2017
Based on previous studies this review presents about double haploid technology and its role in crop improvement. Double haploids are plants those carry two sets of chromosomes that are created from the haploid plants. Different methods such as androgenesis (microspore or anther), gynogenesis (ovule or ovary) haploid inducer lines and wide crosses are used for developing haploid thereby double haploid. Though various chromosome doubling agents found, colchicine has been widely using. The successes of double haploid production relay on different factors like flower parts development stage, culture media, genotype, donor parent growth condition and haploid detection methods. This technology able shortens breeding cycle or time, complete genetic purity, efficient in genetic study, marker development, mutation and transformation better than traditional way of breeding. Generally, understanding DH technology has important contribution in accelerating breeding program for immediate reac...
Haploid and Doubled Haploid Technology
Advances in Botanical Research, 2007
This review of literature is aimed to summarize in-vivo maternal haploid inducer based doubled haploid technology in maize breeding. The three crucial and important steps in this method include the induction of haploids, their identification and subsequent duplication of these haploids to produce doubled haploids lines. With the advent of haploid inducer lines, the production and detection of haploids are possible in the dormant seed stage. The percentage of spontaneous duplication in these haploid plants is very low and germplasm specific. Therefore, the use of artificial duplicating agents is essential to increase the percentage of chromosomal duplication. The cost of developing the DH line can be reduced with the detection of early doubled haploids and the germplasm that supports spontaneous duplication. These developments can replace the long laboratory procedures used for the development of the DH line.
The Recent Development in Technologies for Attaining Doubled Haploid Plants In Vivo
Agriculture
Haploid plants with a doubled set of chromosomes (doubled haploid (DH)) significantly speed up the selection process by the fixation of genetic traits in each locus in the homozygous state within one generation. Doubled haploids are mainly attained by the formation of plants from the cultured gametophytic (haploid) tissues and cells in vitro, or by targeted reduction in the parent chromosome during intra- or interspecific hybridization. Since then, DH has become one of the most powerful tools to support various basic research studies, as well as applied research. This review is focused on the recent development of the production of doubled haploids in vivo and their fundamental bases. The various mechanisms and approaches responsible for the formation of haploids in vivo are discussed, particularly the induction of parthenogenesis by BBM-like proteins, the long constructed Salmon system of wheat, the usage of patatin-like phospholipases MTL/PLA1/NLD, the IG1 system, uniparental geno...
Double haploid production & breeding approaches
2019
Doubled haploid (DH) production has become an important tool in plant breeding largely due to its capacity to produce completely homozygous plants in one generation. Doubled haploids (DHs) are plants derived from a single pollen grain and doubled artificially to form homozygous diploids. A DH individual has two identical homologs, so that the amount of recombination information is equivalent to a backcross. Homozygosity at a particular locus is always desirable feature for crop improvement programs. Breeding is one of the approaches to attain this but it requires minimum 6-7 cropping years. Use of haploid to produce double haploid is one of the potent approaches to overcome this lacuna. Haploid can be either produce through androgenesis or gynogenesis, androgenesis being most used approach. Not only are traits fixed for selection but the multiple generations of inbreeding required using traditional breeding methods are circumvented. However, prior to implementing a DH breeding progr...
Production of double haploids in ornamental crops
2020
Double haploids are the plants, generated spontaneously or induced by chromosome duplication of a haploid plant. They are homozygous at all loci which make them a unique line from the parent in respect of its genome constituent. There is a significant utilization of double haploids in breeding program like production of homozygous lines for cross pollinated crops and mutation breeding. Due to haploidization of chromosomes and again doubling them can produce some novel traits, which cannot be expressed under existing diploid condition. This technique has a significant impact on the improvement program of floricultural crops like chrysanthemum, carnation, petunia, rose, iris, lily, phlox etc. Haploids occur spontaneously at a lower frequency but they can be induced by several methods, such as modified pollination methods in vivo (Wide hybridization, Chromosome elimination, Pollination with irradiated pollen, etc.) and by In vitro culture of immature gametophytes. They are made chromos...
Development of Haploid and Double Haploid in Fruit Crops -A Review
Journal homepage: http://www.ijcmas.com Homozygosity at a particular locus is always desirable feature for crop improvement programs. Breeding is one of the approaches to attain this but it requires minimum 6-7 cropping years. Due to this limitation it is only feasible in annuals. As most of the fruit crops are not annuals, they grow in vegetative phase for 5-6 year before flowering to come. Hence, to achieve homozygosity through breeding approaches is not feasible in them. Use of haploid to produce double haploid is one of the potent approaches to overcome this lacuna. Haploid can be either produce through androgenesis or gynogenesis, androgenesis being most used approach. Here we review the applications of haploid and double haploid production in fruit crops.
Efficiency of anther culture technique in the production of wheat double haploids
Zbornik Matice srpske za prirodne nauke, 2008
The objective of the study was to investigate efficiency of anther culture in the production of spontaneous double haploids from randomly selected heterozygous genotypes of wheat (Triticum aestivum L.). Anthers of 20 F 1 wheat combinations were grown in vitro on a modified Potato-2 medium.
Haploid production technology in wheat and some selected higher plants
Australian Journal of Crop Science, 2011
Haploid plants are very important in various realms of research disciplines such as plant biotechnology, molecular genetics and traditional plant breeding. They provide useful information regarding recombination and genetic control of chromosomal pairing. Haploidy expedites the breeding process thereby increasing the crop yield. Researchers have been working on the haploidy approach for more than half a century. Some crops have shown interesting results in producing haploid cultivars include bread wheat and other crops such as maize, oat and onion. This technique also has useful applications in genetic transformation for generating polyploidy wheat. Wheat cultivars developed from doubled haploid from both anther-culture and maize induction systems have been released for cultivation in all the major continents. Several techniques have been adapted for the production of haploid plants such as anther culture, isolated microspore culture some of which have been discussed in this review....
High production of wheat double haploids via anther culture
Genetika, 2007
High production of wheat double haploids via anther cultur.. Vol. 39, No. 3,[405][406][407][408][409][410][411] Androgenous and regeneration abilities of 14 randomly selected F 1 hybrids of wheat (Triticum aestivum L.) were analyzed. Anthers were grown in vitro on a modified Potato-2 inductive medium.
Plant Cell Reports, 2006
The objective of this work was to produce doubled haploid plants from durum wheat through the induction of androgenesis. A microspore culture technique was developed and used to produce fertile doubled haploid plants of agronomic interest. Five cultivars, one selected line, plus a collection of 20 F 1 crosses between different genotypes of high breeding value were used. Studies on several factors such as pre-treatments and media components were carried out in order to develop a protocol to regenerate green haploid plantlets. Anthers were pre-treated in 0.7 M mannitol. Microspores, from anther maceration, were plated on a C 17 induction culture medium with ovary co-culture. The optimum regeneration medium J25-8 was used. From 35 microspore isolations, 407 green plantlets were obtained. With this technique mature embryos were obtained. Green plants were regenerated from all genotypes used and approximately 67% of them were spontaneously doubled haploids. Some haploids and a very few polyploids plants were obtained. From the 407 plants, 275 were completely fertile and gave enough seeds to be assayed in the field. This protocol could be used complementary to or instead of the intergeneric crossing with maize as an economically feasible method to obtain doubled haploids from most durum wheat genotypes.