Soy Content of Basal Diets Determines the Effects of Supplemental Selenium in Male Mice (original) (raw)
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Effects of dietary selenium on DMBA-induced carcinogenesis in rats fed a diet high in mixed fats
The Journal of nutrition, 1989
The effects of selenium intake on 7,12-dimethylbenz(a)anthracene (DMBA)-induced mammary carcinogenesis were examined in rats fed a diet high in mixed fats and representative of that consumed in North America. Six groups of 20 rats were fed an AIN-76 diet modified to contain 20% fat from lard:corn oil (3:1 wt/wt) and various amounts of selenium (0.1, 0.035, 0.1, 1.0, 2.0 or 4.0 mg Se/kg diet). At wk 5, animals in groups 2-6 were dosed with 4.32 mg of DMBA. Serum clinical parameters and the activities of plasma selenium-dependent and total glutathione peroxidase (GSHPx), erythrocyte GSHPx and superoxide dismutase (SOD) were determined every 4 wk for 25 wk. The extent of lipid peroxidation was determined by measuring urinary malondialdehyde during wk 13 and 24, and erythrocyte malondialdehyde at wk 25. Erythrocyte GSHPx was found to be a better indicator of selenium status than plasma activity, while SOD did not vary with dietary selenium. The group of animals fed 4.0 mg Se/kg diet had...
Effects of selenium deficiency on fatty acid metabolism in rats fed fish oil-enriched diets
Journal of Trace Elements in Medicine and Biology, 2004
The hepatic fatty acid metabolism was investigated in rats stressed by selenium deficiency and enhanced fish oil intake. Changes in the composition of lipids, peroxides, and fatty acids were studied in the liver of rats fed either a Sedeficient (8 mg Se/kg) or a Se-adequate (300 mg Se/kg) diet, both rich in n-3 fatty acid-containing fish oil (100 g/kg diet) and vitamin E (146 mg alpha-tocopherol/kg diet). The two diets were identical except for their Se content. Se deficiency led to a decrease in hair coat density and quality as well as to changes in liver lipids, individual lipid fractions and phospholipid fatty acid composition of the liver. The low Se status did reduce total and reduced glutathione in the liver but did not affect the hepatic malondialdehyde level. In liver phospholipids (PL), Se deficiency significantly reduced levels of palmitic acid [16:0], fatty acids of the n-3 series such as DHA [22:6 n-3], and other long-chain polyunsaturates C-20-C-22, but increased n-6 fatty acids such as linoleic acid (LA) [18:2 n-6]. Thus, the conversion of LA to arachidonic acid was reduced and the ratio of n-6/n-3 fatty acids was increased. As in liver PL, an increase in the n-6/n-3 ratio was also observed in the mucosal total fatty acids of the small intestine. These results suggest that in rats with adequate vitamin E and enhanced fish oil intake, Se deficiency affects the lipid concentration and fatty acid composition in the liver. The changes may be related to the decreased levels of selenoenzymes with antioxidative functions. Possible effects of Se on absorption, storage and desaturation of fatty acids were also discussed.
Absorption and retention of 75SeO3 in relation to soybean protein intake in the rat
Nutrition Research, 1982
The absorption and retention of 75Se, given as an oral dose of 75Se03, was studied in young male rats receiving different levels and sources of soy protein, with and without selenite and methionine supplementation. Rats fed a protein-free diet had a higher cumulative urine 75Se excretion and a s l i g t l y lower 7SSe absorption and 75Se retention than rats fed diets containing I0% protein supplied as soya flour. Results indicated that supplementation with selenite decreased the fractional absorption andretention of selenium, but the overall effect was a marked increase in the total amount of selenium ingested, absorbed and retained. Methionine supplementation of a diet based on soya increased growth and PER: i t also decreased slightly cumulative feces 75Se excretion and increased 75Se absorption, but only in rats fed diets supplemented with selenium. The present findings are consistent with the view that selenium homeostasis in the rat is maintained largely through changes in the urinary excretion of selenium and they show that an inadequate protein diet reduces the efficiency of retention of absorbed selenite.
Biological Trace Element Research
Twenty-nine obese female Zucker rats (fa/fa) were fed with a laboratory chow supplemented or not with a selenium-rich yeast (Selenion), or Selenion + vitamin E, or vitamin E alone. Twelve lean female Zucker rats (Fa/Fa) of the same littermates fed with the same diet were used as control. After 32 wk of diet, obesity induced a large increase in plasma insulin and lipid levels. A significant decrease in the plasma vitamin E/triglycerides ratio (p<0.005) and an increase in plasma thiobarbituric reactive substances (TBARS) (p<0.005) were also observed. Plasma selenium and vitamin E increased in all supplemented rats. The plasma insulin level was decreased by selenion supplementation and the vitamin E/triglycerides ratio was completely corrected by double supplementation with Selenion + vitamin E. TBARS were also efficiently decreased in two obese groups receiving vitamin E. In plasma, adipose tissue and aorta, obesity induced an increase in palmitic acid (C16:0), a very large incr...
BioMed research international, 2017
The response of nutrient status and biochemical processes in (i) Wistar and (ii) spontaneously hypertensive (SHR) rats upon dietary intake of selenium- (Se-) enriched defatted rapeseed (DRS) and/or vitamin E fortification was examined to assess the health benefit of DRS in animal nutrition. Twenty-four individuals of each type of rat were used: The control group was fed with an untreated diet (Diet A). In Diets B and C, soybean meal was replaced with defatted DRS, which comprised 14% of the total diet. The selenized DRS application resulted in ~3-fold increase of Se content in the diet. Diet C was also fortified with the addition of vitamin E, increasing the natural content by 30%. The Se content of the blood and kidneys tended to increase in the DRS groups, where the changes were significant (P < 0.05) only in the case of SHR rats. The iodine (I) content and the proportion of iodide in rat livers indicated a lower transformation rate of iodide into organoiodine compounds compare...
Journal of Animal and Feed Sciences, 2007
The infl uence of diets enriched in 2% CLA isomer mixture (as CLAmix A or CLAmix B) and/or Se as selenate (Se VI) or selenized yeast (Se-Y) on the concentration of fatty acids (FA) and amino acids (AA) in the liver and femoral muscles was studied on 8 groups of 7-8 rats aged 8 weeks. Rats were fed a basal diet for 29 days or diets enriched with two combinations of 2% CLAmix A with/without 2 ppm Se (as Se VI) or 2% CLAmix B with/without 1.2 ppm Se (as Se-Y). The dietary CLAmix B containing the higher concentration of trans10cis12CLA (t10c12CLA) resulted in a higher decrease of body weight gain (BWG) (P<0.1) than the dietary CLAmix A with the lower concentration of t10c12CLA. The diet enriched in Se VI reduced the BWG (P<0.05) in rats. The dietary CLAmix A lowered the concentration of saturated FA (SFA), atherogenic SFA and thrombogenic SFA in the liver and muscles. The value of the Δ9-desaturase index decreased in the liver and muscles of rats fed the diet enriched in CLAmix A or CLAmix B compared with that in the control rats. There were signifi cantly higher (P<0.01) concentrations of c9t11CLA, t10c12CLA, ccCLA and ttCLA in the muscles compared with the concentrations of these isomers in the liver of rats fed the diets enriched in CLAmix A or CLAmix B , regardless the presence of extra Se as Se VI or Se-Y. The ratio (R t10c12/c9t11) of t10c12CLA and c9t11CLA in the liver and muscles of rats fed the diets enriched in CLA isomers was, regardless of the presence of Se, smaller compared with the values of R t10c12/c9t11 in the dietary CLA isomers. The concentrations of CLA isomers tended to or signifi cantly increased in the liver and muscles of rats fed diets containing Se and CLA isomers compared with rats fed the diets enriched in CLA isomers. The concentrations of PUFAn-3, PUFAn-6, PUFA and the ratio of PUFA/SFA decreased in the muscles of rats fed the diet with CLAmix A. The results suggest that the dietary CLAmix A containing the higher concentration of ttCLA, regardless of the presence of Se VI , more effi ciently reduced the magnitude of lipoprotein synthesis in muscles than the diet containing CLAmix B with/without Se-Y,
Selenium Supplementation Alters Hepatic Energy and Fatty Acid Metabolism in Mice
The Journal of nutrition, 2018
Human and animal studies have raised concerns that supplemental selenium can increase the risk of metabolic disorders, but underlying mechanisms are unclear. We used an integrated transcriptome and metabolome analysis of liver to test for functional pathway and network responses to supplemental selenium in mice. Male mice (8-wk-old, C57BL/6J) fed a standard diet (0.41 ppm Se) were given selenium (Na2SeO4, 20 μmol/L) or vehicle (drinking water) for 16 wk. Livers were analyzed for selenium concentration, activity of selenoproteins, reduced glutathione (GSH) redox state, gene expression, and high-resolution metabolomics. Transcriptomic and nontargeted metabolomic data were analyzed with biostatistics, bioinformatics, pathway enrichment analysis, and combined transcriptome-metabolome-wide association study (TMWAS). Mice supplemented with selenium had greater body mass gain from baseline to 16 wk (55% ± 5%) compared with controls (40% ± 3%) (P < 0.05); however, no difference was obser...