Distribution Genotype High Risk (HR) And Low Risk (LR) Human Papillomavirus (HPV) at Condyloma Acuminata (original) (raw)
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Berkala Ilmu Kesehatan Kulit dan Kelamin, 2022
Background: Condyloma acuminatum (CA) or genital warts is sexually transmitted infection resulting from Human papillomavirus (HPV). It is estimated that CA occurs in >80% of reproductive age in many countries, with the most common types being low risk types HPV 6 and 11, but sometimes could be combined with high-risk types are HPV 16, 18. Gen E6 is an oncoprotein that cooperates with E7 to provide a suitable environment for viral DNA replication and inhibits cellular apoptotic processes. The E6 gene has characteristic properties as a p53 tumor suppressor protein. Purpose: To analyse the high-risk types HPV 16 and 18 in CA patients. Methods: This study was a cross-sectional, observational analytic study that assessed data collection concurrently over time. Specimens were patients diagnosed with condyloma acuminatum, biopsies were performed, and immunohistochemical (IHC) staining was also performed to examine E6 of high risk types HPV 16 and 18. The sampling technique used sequential sampling, and the inclusion criteria included patients who came to our hospital for treatment. Result: Twenty patients with CA were include in this study consist of 11 male and 9 female, meanwhile 9 subjects in homosexual group and 11 subjects in heterosexual group. Based on IHC examination 33% cases were detected of high-risk types HPV 16 and 18 especially among homosexual group. According to t-test obtained significant result of highrisk types and low-risk types of HPV from IHC staining (p=0.038). Conclusion: Condyloma acuminatum is a sexually transmitted infection that commonly caused by low-risk types HPV 6 and 11, but this study showed high risk types HPV16 and 18 also found in MSM (Male Sex with Male) patients with CA, so that it is necessary to be aware of the possibility of transformation to malignancy.
Journal of clinical microbiology, 1999
Condylomata acuminata, or genital warts, are proliferative lesions of genital epithelium caused by human papillomavirus (HPV) infection. HPV types 6 and 11 are most often detected in these lesions. Genital lesions consistent with exophytic condylomata acuminata were removed by excision biopsy from 65 patients, 41 of whom were otherwise healthy individuals (control group) and 24 of whom had conditions known to cause immunosuppression. Histologically, the majority of the lesions were typical condylomata acuminata. Three lesions removed from immunosuppressed individuals also contained foci of moderate to severe dysplasia (intraepithelial neoplasia grade II/III). A recently developed PCR and reverse blot strip assay was used to determine the specific HPV types present in the genital lesions. With a set of oligonucleotide primers based on the same primer binding regions used for the MY09 and MY11 primer pair, this PCR assay detects the presence of 27 HPV types known to infect the genital...
2016
In order to identify differences in clinical, histopathology and virology data of patients with external condylomata acuminata (ECA) by HIV status, 47 HIV positive patients and 42 HIV negative patients were enrolled. Socio-demographic and clinical data was recorded. Histopathology study and HPV detection (Roche Linear Array genotyping assay) were performed. HIV positive patients were mainly single, MSM, with history of STI, multiple sexual partners, receiving antiretroviral therapy and with recurrent peri-anal ECA. HPV-16 and HPV-61 were associated to this group while HPV-6 was found in HIV negative patients with ECA. Intraepithelial neoplasia (IEN) was found in 5.6% of patients, associated to HIV and HPV-16. We found differences in socio-demographic characteristics and HPV genotypes in patients with ECA by HIV status. The association of high grade IEN with HIV infection makes evident the need of defined parameters as markers for early cancer diagnosis.
Journal of Infectious Diseases, 1994
Condylomata acuminata, or genital warts, are proliferative lesions of genital epithelium caused by human papillomavirus (HPV) infection. HPV types 6 and 11 are most often detected in these lesions. Genital lesions consistent with exophytic condylomata acuminata were removed by excision biopsy from 65 patients, 41 of whom were otherwise healthy individuals (control group) and 24 of whom had conditions known to cause immunosuppression. Histologically, the majority of the lesions were typical condylomata acuminata. Three lesions removed from immunosuppressed individuals also contained foci of moderate to severe dysplasia (intraepithelial neoplasia grade II/III). A recently developed PCR and reverse blot strip assay was used to determine the specific HPV types present in the genital lesions. With a set of oligonucleotide primers based on the same primer binding regions used for the MY09 and MY11 primer pair, this PCR assay detects the presence of 27 HPV types known to infect the genital tract. All but two condylomata acuminata contained either HPV type 6 or 11. The predominant type in the lesions from control patients was HPV 6, while lesions from immunosuppressed types most often contained HPV 11. Condylomata acuminata from immunosuppressed patients contained significantly more overall HPV types than lesions from the control group. HPV types associated with an increased risk of dysplasia (high-risk types) were detected in 42 (64.6%) of the total of 65 specimens; 18 (43.9%) specimens were detected in the 41 otherwise healthy individuals, and 24 (100%) specimens were detected in the 24 immunosuppressed patients. HPV 16 was the most common high-risk type detected, found in 21 of 65 (32.3%) specimens. After HPV types 6 and 11, HPV types 53 and 54 were the most frequently detected low-risk HPV types. This study demonstrates that a high percentage of condylomata acuminata lesions contain multiple HPV types, including types associated with a high risk of dysplastic abnormalities. Further studies are needed to determine the influence these additional HPV types have on the epidemiology of genital tract HPV infections and the natural history of condylomata acuminata, especially in immunosuppressed patients.
Biotechnology & Biotechnological Equipment, 2006
Oral condyloma acuminatum is a papillomatous lesion that is transmitted most often sexually and associated with the human papilloma virus (HPV). This report describes a case of a 30-year-old woman with multiple oral condylomata acuminata, located on the lateral edges and on the dorsum of the tongue. The incisional biopsy showed histological features compatible with those of condyloma acuminatum. E6 viral oncogene of HPV types 6, 11, 16, and 33 was identified by means of polymerase chain reaction (PCR). The lesions were removed by radiodissecation and treated topically with trichloroacetic acid (80% and 40%) and interferon-alpha-2a. No recurrence was evident 8 months after the treatment. We report this case not only for the simultaneous presence of low-and highrisk HPV types in one patient and the rarity of the condition, but also for the good response to the combined surgical and medical treatment that we observed.
The Genotype of Human Papilloma Virus of Male Patient with Anogenital Warts
Folia Medica Indonesiana
Human papillomavirus (HPV) infection is one of the most common sexually transmitted infections. HPV high risk (HPVHR) were HPV16,18 related with invasive penile carcinomas, and HPV low risk (HPVLR) were HPV6,11 related to anogenital warts. Male infection is usually asymptomatic that it would be explain increasing the incidence of HPV associated cancers. Identification HPV genotype is very important for predicting the development of the diseases, to be benign or malignant cancer. The objective of this study was to identify the genotype of HPV that infect men with anogential warts. This research used 12 biopsy specimens from men patient with anogenital warts at Outpatient clinic of Department Dermatology and Venereology, Dr. Soetomo General Hospital period 2016-2017. The specimens were diagnozed by pathologist and HPV gentoyping was done to detect 40 HPV genotype including HPVHR and HPVLR. The result showed that 58% (7/12) were positive for HPVLR and 42% (5/12) were positive for HPV L...
Human Papillomavirus High-risk Type Infection on Perianal Giant Condyloma Acuminatum
Bioscientia Medicina : Journal of Biomedicine and Translational Research
Background: Giant condyloma acuminatum (GCA) is a large form of condyloma acuminatum that become locally invasive, destructive, but non-metastasizing, caused by human papillomavirus (HPV). Giant condyloma is a rare disease, with only 0.1% incidence in the general population. Most commonly caused by HPV type 6 and 11 infections, in this case, reported HPV high-risk type. Case presentation: A 20-years-old man, the main complaint is enlarging, easy bleeding perianal tumor with pruritus and constipation for 2 months. Physical examination found skin-colored, verrucous surface. Some have smooth surface, cauliflower-like tumor on perianal region, size 7x4x2cm. Syphilis and HIV Serology laboratory tests are non-reactive. Koilocytes appear on histopathologic examination. High-risk HPV type detected on HPV genotyping. This patient was diagnosed as GCA. Topical agent 25% podophyllin tincture applied to the lesions once a week. Improvement appears after eighth-time therapy. Discussion: High-ris...
Detection of specific HPV subtypes responsible for the pathogenesis of condylomata acuminata
Virology Journal, 2013
Background The low-risk human papillomavirus types 6 and 11 are responsible for approximately 90% of anogenital wart cases, with approximately 190,000 new and recurrent cases reported in the UK in 2010. The UK has recently selected the quadrivalent HPV vaccine, which conveys protection against both HPV6 and HPV 11, as part of its immunisation programme for 2012 and it is expected that this will reduce disease burden in the UK. The aims of the study were to evaluate current strategies used for the monitoring of HPV infection in genital warts and to assess the suitability of laser-capture microdissection (LCM) as a technique to improve the understanding of the natural history of HPV types associated with genital wart lesions. Methods DNA and RNA were extracted from whole wart, surface swabs and LCM sections from 23 patients. HPV types present were determined using the Linear Array HPV Genotyping Test (Roche), with HPV DNA viral load and mRNA expression investigated using qPCR and qRT-...
Genotype distribution of human papillomavirus in anogenital warts in Guilan province, northern Iran
International Journal of Dermatology, 2021
Background: Anogenital warts are the most prevalent sexually transmitted disease worldwide and are caused by human papillomavirus (HPV). There are more than 100 different types of HPV, of which certain types make the patient more susceptible to malignant neoplasms. This study aimed to determine the frequencies of specific types of HPV in anogenital warts. Methods: This study included 157 patients diagnosed with anogenital warts that underwent HPV DNA detection using polymerase chain reaction (PCR) with INNO-LiPA genotyping assay. Results: Of 157 patients with anogenital warts, 122 samples (77.7%) were positive for HPV, with some patients showing mixed HPV subtypes. HPV 6 (n = 99; 68.3%) and HPV 11 (n = 13; 8.9%) were the most prevalent types. 101 patients were male (64.3%). The mean age was 32.02 ± 10.06 years (range: 2 to 72 years). Most patients were infected with the low-risk types (80%); the high-risk HPV type 16 was significantly more common among women (P = 0.0001). Conclusion: This study showed the higher relative frequency of HPV6 and HPV11 in anogenital warts in Guilan, northern Iran. The frequency of genital HPV infection appears to be higher among men, but the frequency of high-risk types seems to be higher among women.
The American journal of surgical pathology, 2017
Laser capture microdissection-polymerase chain reaction (LCM-PCR) supported by p16 was used for the first time to demonstrate human papillomavirus (HPV) DNA in histologically specific penile lesions, which were as follows: squamous hyperplasia (12 lesions, 10 patients), flat lesions (12 lesions, 5 patients), condylomas (26 lesions, 7 patients), penile intraepithelial neoplasia (PeIN) (115 lesions, 43 patients), and invasive squamous cell carcinomas (26 lesions, 26 patients). HPV was detected by whole-tissue section and LCM-PCR. LCM proved to be more precise than whole-tissue section in assigning individual genotypes to specific lesions. HPV was negative or very infrequent in squamous hyperplasia, differentiated PeIN, and low-grade keratinizing variants of carcinomas. HPV was strongly associated with condylomas, warty/basaloid PeIN, adjacent flat lesions, and warty/basaloid carcinomas. A single HPV genotype was found in each lesion. Some condylomas and flat lesions, especially those ...