Evaluation of Antibacterial Behaviour of Silver Nanoparticles (original) (raw)
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Abstract In recent years, skin and soft-tissue infections (SSTIs), particularly due to multidrug-resistant pathogens areincreasingly being encountered in clinical settings. Due to the development of antibiotic resistance and theoutbreak of infectious diseases caused by resistant pathogenic bacteria, the pharmaceutical companies and theresearchers are now searching for new unconventional antibacterial agents. Recently, in this fieldnanotechnology represents a modern and innovative approach to develop new formulations based on metallicnanoparticles with antimicrobial properties. The bacterial growth curve, minimum inhibitory concentration (MIC),and minimum bactericidal concentration (MBC) of silver nanoparticles (Ag-NPs) towards Staphylococcus aureusATCC25923, methicillin-sensitive S. aureus (MSSA), and methicillin-resistant S. aureus (MRSA) were examinedin this study. The experiment results showed that the lowest MIC and MBC of Ag-NPs to MRSA was 12.5 μg/mland 25 μg/ml, respectively....
Antibacterial effect of silver nanoparticles on Staphylococcus aureus
BioMetals, 2011
Microorganisms play an important role in toxic metal remediation through reduction of metal ions. Studies demonstrated that silver ions may be reduced extracellularly using Fusarium oxysporum to generate stable gold or silver nanoparticles in water. These particles can be incorporated in several kinds of materials such as cloths. These cloths with silver nanoparticles are sterile and can be useful in hospitals to prevent or to minimize infection with pathogenic bacteria such as Staphylococcus aureus. In this work, the extracellular production of silver nanoparticles by F. oxysporum and its antimicrobial effect when incorporated in cotton fabrics against S. aureus were studied. In addition, all effluent was bioremediated using treatment with C. violaceum. The results showed that cotton fabrics incorporated with silver nanoparticles displayed a significant antibacterial activity against S. aureus. The effluent derived from the process was treated with C. violaceum and exhibited an efficient reduction in the silver nanoparticles concentration. In conclusion, it was demonstrated the application of biological synthesis to silver nanoparticles production and its incorporation in cloths, providing them sterile properties. Moreover, to avoid any damage to the environment the effluent containing silver nanoparticles can be treated with cyanogenic bacterial strains.
Silver nanoparticles against Staphylococcus aureus resistant to meticillin: a literature review
2019
The Methicillin-resistant Staphylococcus aureus (MRSA) represents a critic risk to public health because it has resistance to multiple antimicrobial agents and is one of the most common nosocomial pathogens. Consequently, researches to develop or modify antimicrobial compounds to improve bactericidal against the potential strains of MRSA have been grouwing. In this context, this study aimed to conduct a literature review about the antimicrobial activity against MRSA of silver nanoparticles (AgNPs) and discuss the main results. The survey was conducted in the ScienceDirect database, where the descriptors used were “silver nano * and MRSA”. Based on the results, we can conclude that the antimicrobial effects of the AgNPs against MRSA are promising in several areas, as these did not cause damage to healthy cells and caused no observable side effects.
NANOTECHNOLOGY IN MEDICINE AND ANTIBACTERIAL EFFECT OF SILVER NANOPARTICLES
Nanotecnología
Nanotechnology is expected to open some new aspects to fight and prevent diseases using atomic scale tailoring of materials. The ability to uncover the structure and function of biosystems at the nanoscale, stimulates research leading to improvement in biology, biotechnology, medicine and healthcare. The size of nanomaterials is similar to that of most biological molecules and structures; therefore, nanomaterials can be useful for both in vivo and in vitro biomedical research and applications. The integration of nanomaterials with biology has led to the development of diagnostic devices, contrast agents, analytical tools, physical therapy applications, and drug delivery vehicles. In all the nanomaterials with antibacterial properties, metallic nanoparticles are the best. Nanoparticles increase chemical activity due to crystallographic surface structure with their large surface to volume ratio. The importance of bactericidal nanomaterials study is because of the increase in new resistant strains of bacteria against most potent antibiotics. This has promoted research in the well known activity of silver ions and silver-based compounds, including silver nanoparticles. This effect was size and dose dependent and was more pronounced against gram-negative bacteria than gram-positive organisms.
Research review paper Silver nanoparticles as a new generation of antimicrobials
Silver has been in use since time immemorial in the form of metallic silver, silver nitrate, silver sulfadiazine for the treatment of burns, wounds and several bacterial infections. But due to the emergence of several antibiotics the use of these silver compounds has been declined remarkably. Nanotechnology is gaining tremendous impetus in the present century due to its capability of modulating metals into their nanosize, which drastically changes the chemical, physical and optical properties of metals. Metallic silver in the form of silver nanoparticles has made a remarkable comeback as a potential antimicrobial agent. The use of silver nanoparticles is also important, as several pathogenic bacteria have developed resistance against various antibiotics. Hence, silver nanoparticles have emerged up with diverse medical applications ranging from silver based dressings, silver coated medicinal devices, such as nanogels, nanolotions, etc.
2017
Staphylococcus aureus is a leading cause of nosocomial infections and the etiologic agent of a wide range of diseases associated with significant morbidity and mortality. Some of the diseases mediated by this species include endocarditis, osteomyelitis, toxic shock syndrome, food poisoning, and skin infections. Biofilms are well organized and complex aggregate of microorganisms, surrounded by a protective matrix of exo-polysaccharides and can adhere to each other on various surfaces. Because of increase the drug resistance and the side effect of over use of antibiotic, it is necessary to find a suitable agent to reduce the growth of microorganism. Biofilm are known as a significant problem because biofilm formation protects pathogenic bacteria against antibiotics and is one of the main causes of development of chronic infections. Thus biofilm is the main cause of drug resistance in microbial strains. These strains cause pathogenicity to a maximum extent and thus cause resistance aga...
Silver nanoparticles as a new generation of antimicrobials
Silver has been in use since time immemorial in the form of metallic silver, silver nitrate, silver sulfadiazine for the treatment of burns, wounds and several bacterial infections. But due to the emergence of several antibiotics the use of these silver compounds has been declined remarkably. Nanotechnology is gaining tremendous impetus in the present century due to its capability of modulating metals into their nanosize, which drastically changes the chemical, physical and optical properties of metals. Metallic silver in the form of silver nanoparticles has made a remarkable comeback as a potential antimicrobial agent. The use of silver nanoparticles is also important, as several pathogenic bacteria have developed resistance against various antibiotics. Hence, silver nanoparticles have emerged up with diverse medical applications ranging from silver based dressings, silver coated medicinal devices, such as nanogels, nanolotions, etc.
Antimicrobial effects of silver nanoparticles
Nanomedicine: Nanotechnology, Biology and Medicine, 2007
The antimicrobial effects of silver (Ag) ion or salts are well known, but the effects of Ag nanoparticles on microorganisms and antimicrobial mechanism have not been revealed clearly. Stable Ag nanoparticles were prepared and their shape and size distribution characterized by particle characterizer and transmission electron microscopic study. The antimicrobial activity of Ag nanoparticles was investigated against yeast, Escherichia coli, and Staphylococcus aureus. In these tests, Muller Hinton agar plates were used and Ag nanoparticles of various concentrations were supplemented in liquid systems. As results, yeast and E. coli were inhibited at the low concentration of Ag nanoparticles, whereas the growth-inhibitory effects on S. aureus were mild. The free-radical generation effect of Ag nanoparticles on microbial growth inhibition was investigated by electron spin resonance spectroscopy. These results suggest that Ag nanoparticles can be used as effective growth inhibitors in various microorganisms, making them applicable to diverse medical devices and antimicrobial control systems.
Annals of Tropical Medicine and Public Health, 2019
Silver nanoparticles (AgNPs) are of special concern as a result of their unique chemical, physical and biological characteristics. It has become an attractive alternative to antibiotics due to their broad-spectrum antimicrobial activity. The study aimed to determine the antibacterial activity of AgNPs against S. aureus bacteria and the effect of AgNPs on the viability of normal cell line (vero cell). A total of 70 clinical samples (wound and vagina swab, stool and urine) were used in this study. Bacterial isolates were subjected to the microscopical, cultural and biochemical evaluation. AgNPs were prepared and checked for their antimicrobial activity by the use of various concentrations employing agar dilution method. In addition, the effect of different concentrations of AgNps on a viability of vero cells was examined. The results showed that out of 70 clinical samples, 11 (15.7%) isolates were Staphylococcus aureus. AgNps showed high activity against S. aureus at concentrations (100 μg/ml and 200μg/ml). It was found that there was no effect of AgNPs on the viability of the normal vero cells at (≤ 250 μg/ml) concentration, but they have cytotoxic effect on the viability of the these cells at high concentrations. This study concluded that AgNPs possess good antimicrobial activity and the concentrations that maintain the cell viability could be used as an alternative therapy to treat S. aureus infections.
International Research Journal Of Pharmacy
Nanotechnology is emerging as a new interdisciplinary field combining microbiology, Chemistry, physics, and material science. Recent advances promise developments in the synthesis, modification and practical applications of nanoparticles (NPs). Nanoparticles were excellent antibacterial agents with potential clinical applications. Sliver Nanoparticles have been successfully used in a wide range of applications including wound dressing, protective clothing, antibacterial surfaces, food preservation, and cosmetics as biocidal and disinfecting agents. The aim of this study was to investigate the mechanism of silver nanoparticle action against of methicillin-resistant Staphylococcus aureus. The Gram-positive methicillin-resistant Staphylococcus areas were used to evaluate the antibacterial activities of silver nanoparticles (Ag NPs). The growth rate of methicillin-resistant Staphylococcus aureus was investigated under varying Ag NPs concentrations by scanning electron microscope (SEM) & acridine orange /ethidium bromide (AO-EtBr) staining. The Quantitative Real-time PCR experiment showed that the mecA gene from the bacterial cells treated with Ag-NPs was downregulated compared to that in the untreated cells.