ANTIMICROBIAL ACTIVITY OF AQUEOUS, ETHANOLIC AND METHANOLIC LEAF EXTRACTS FROM ACACIA SPP. AND Eucalyptus nicholii (original) (raw)

Evaluation of Antimicrobial Potentiality of Aqueous Ethanolic Leaf Extract of Acacia Arabica Extract

2019

INTRODUCTON Scientific communities using many efforts have been made to discover new antimicrobial compounds from various kinds of sources such as micro-organisms, animals, and plants. One of such resources is folk medicines. Systematic screening of herbal plant may result in the discovery of novel effective compounds. They using the multi-drug resistant strains of bacteria and the recent appearance of strains with reduced susceptibility to ABSTRACT The present study is the continuation of a program aimed at investigation of antimicrobial properties of Acacia arabica extract to justify the traditional claim endowed upon this herbal drug as a rasayana in Ayurveda. The antimicrobial activity of Acacia arabica was evaluated according to the disk diffusion method by using Gram positive; B. subtilius, S. aureus and S. epidermidis and Gram Negative; E. coli S. flexineri, P. aeruginosa bacteria. This study show that methanolic fruits extract of Acacia arabica Linn inhibits the growth of mi...

Antimicrobial Activity of Bark extract from Acacia

Acacia jacquemontii was assessed for active principles to ascertain the rationale for its use in traditional medicine. Preliminary phytochemical screening of the stem bark extracts showed that it possessed the active principles -alkaloids, glycosides, saponins, terpenoids and tannins. The antimicrobial activity of the extracts was assayed against pathogenic strains of Bacillus cereus, Bacillus pumilus, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, S. pyrogenes, and Candida albcans using the agar diffusion method. The plant extract exhibited antimicrobial activity against all the test microorganisms. B. cereus and B. pumilus were the most susceptible to the plant extract while Candida albicans was the most resistant. The minimum inhibitory concentration of the stem bark extract of the plant ranged between 30 and 50 mg/ml while the minimum bactericidal concentration ranged between 35 and 60 mg/ml. A. jacquemontii could be a potential source of antimicrobial agents.

Antibacterial potential of crude leaf extracts of Eucalyptus camaldulensis against some pathogenic bacteria

African Journal of Plant Science, 2010

The antibacterial activity of the crude leaf extracts of Eucalyptus camaldulensis were determined using the agar well diffusion method against clinical isolates of Escherichia coli, Staphylococcus aureus, Salmonella typhi, Proteus mirabilis andKlebsiella pneumoniae. At an extract concentration of 50 mg/ml, the growths of all the pathogenic bacteria were arrested, though to varying degrees. The least activity in terms of zones of growth inhibition was shown by aqueous extract against E. coli (7 mm), K. pneumoniae (9 mm), P. mirabilis (13 mm), S. typhi (12 mm) and S. aureus (12 mm) while the highest was demonstrated by the acetone extract, with a recorded zone diameter for E. coli (12 mm), K. pneumoniae (13 mm), S. typhyi (14 mm), P. mirabilis (15 mm) and S. aureus (14 mm). The effects of pH and temperature on the efficacy of the crude drug extracts were also determined. The effectiveness of the extracts were more pronounced under alkaline conditions and lower temperatures. Minimum i...

Antibacterial activity of ethanolic and aqueous extracts of Acacia aroma Gill. ex Hook et Arn

Life Sciences, 2004

The purpose of the present study was to investigate the antibacterial activity of seven ethanolic extracts and three aqueous extracts from various parts (leaves, stems and flowers) of A. aroma against 163 strains of antibiotic multi-resistant bacteria. The disc diffusion assay was performed to evaluate antibacterial activity of the A. aroma crude extracts, against several Gram-positive bacteria (E.

Antimicrobial Effects of Leaves of Eucalyptus camaldulensis on Some Microbial Pathogens

European Journal of Medicinal Plants, 2016

Background: Plants are important sources of medicinal materials and have been in use since ancient times. Although numerous plants have been explored for their medicinal properties, there still remains much to be studied. Aim: To evaluate the phytochemical constituents of Eucalyptus camaldulensis extracted using ethanol, methanol and petroleum ether and the antimicrobial activity of the leaf extracts. Place and Duration of Study: The study was conducted in Jos, Plateau State, Nigeria over a period of 18 months. Methodology: Methanol, ethanol and petroleum ether extracts of E. camaldulensis leaves were obtained and phytochemical constituents were determined following standard procedures. The antimicrobial effect of the extracts obtained was tested against three bacterial species including Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa and two fungi namely Penicillium expansum and Candida albicans using the agar well diffusion method, at concentrations of 400, Chuku et al.; EJMP, 14(2): 1-8, 2016; Article no.EJMP.25759 2 200, 100 and 50 mg/ml. Minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and minimum fungicidal concentration (MFC) were also determined. Results: The phytochemical analysis of the leaf extracts revealed that they contained alkaloids, tannins, flavonoids, saponins, carbohydrates, steroids and cardiac glycosides. All the test organisms (both bacteria and fungi) were inhibited by each of the extracts at concentrations of 400, 200, 100 and 50 mg/ml with variations. The highest concentration (400 mg/ml) of ethanol extract showed the highest inhibition for C. albicans (27 mm) and B. subtilis (27 mm), while those of methanol and petroleum ether were most effective against C. albicans (27.7 mm) and B. subtilis (27 mm). The MIC ranged between 50 mg/ml and 200 mg/ml, while MBC and MFC ranged between 100 mg/ml and 400 mg/ml for all extracts. The inhibitory effects of the plant extracts were comparable to that of commercially available antibiotics used as controls. Conclusion: The leaf extract of E. camaldulensis could be a better option in the treatment of infections caused by test organisms studied if properly processed and harnessed.

Evaluation of Antimicrobial Activity of Acacia etbaica Water Extract Leafs against Some Pathogenic Microorganisms

Journal of Biomedical Research & Environmental Sciences, 2021

The antimicrobial activity about water extract of Acacia etbaica was examined by using agar well diffusion methods against five gram-positive and negative bacteria[Staphylococcus aureus (ATCC653-8), Pseudomonas aeruginosa MTCC2453, Bacillus cereus (ATCC6633), Escherichia coli MTCC739, and one local isolate (Staphylococcus epidermides)] in addition to Candida albicans (ATCC2019). this results designated that the water extract of Acacia etbaica possess antimicrobial efficacy against all tested microorganisms either (gram-positive and negative bacteria) or fungi (Candida albicans).Whereas the gram-positive bacteria (S. aureus, S. epidermides and B. cereus) with inhibition zones (21, 19.5 and 16.5) respectively was more sensitive than gram-negative bacteria (P. aeruginosa and E. coli) and C. albicans with inhibition zones 16 and 14.5 respectively. The antimicrobial effect was directly proportional with concentration of water extract where the highest inhibition zone at higher concentrat...

Antibacterial effect of the leaves of Eucalyptus globulus against clinical bacterial isolates

2019

Antibiotic resistance being a major threat to public health, instigated the search for new antimicrobial agents especially in the recognized medicinal plants. In this study the antibacterial effect of the leaves of the plant: <em>Eucalyptus globulus, </em>used in herbal medicine, against clinical Gram positive and Gram negative clinical bacterial isolates was studied. This antibacterial effect was determined using the well agar diffusion and agar dilution methods. Using the well agar diffusion method, the aqueous extract of <em>E. globulus </em>exhibited a weak inhibitory effect evident at a volume of 200 μl against the methicillin resistant and methicillin sensitive <em>Staphylococcus aureus </em>isolates and 2 of the 3 <em>Enterococcus faecalis </em>isolates and at a volume of 300 μl for the <em>Acinetobacter baumannii </em>isolates. On the other hand, the methanolic extract of the plant showed a notable inhibitory activi...

Differential Antibacterial Activity of the Various Crude Leaf Extract of Eucalyptus Offensiveness Against Selected Pathogenic Bacterial Strains

The antibacterial activities of Eucalyptus officinalis extracts were studied. The antibacterial efficiency of the above mentioned plant was evaluated according to agar diffusion and broth dilution methods by using Escherichia coli, Staphylococcus aureus, Streptococcus pneumoniae, Proteus mirabilis and Pseuomonas aeruginosa clinical isolates and typed cultures. The most susceptible bacteria were E. coli followed by S. aureus while the most resistant bacteria were Pseud. aeruginosa followed by P. mirabilis and Strept. Pneumoniae. From the screening experiment, the hot water extract produced the highest inhibitory effect 17.22+0.14 and 17.63+0.06 against the clinical isolated and typed culture respectively, hence this plant can be further subjected to isolation of the therapeutic antimicrobials and pharmacological evaluation. Keywords: antibacterial activity, bacterial strains, crude leaf extract, Eucalyptus officinalis, pathogenic,

STUDIES ON ANTIBACTERIAL PROPERTY OF EUCALYPTUS - THE AROMATIC PLANT

Eucalyptus is a plant that is used in traditional medicine for the treatment as antimicrobial drugs. To calculate the potential of the plant, the antimicrobial activities of extracts of the leaves were evaluated against some common bacteria. The dried powdered leaves were extracted using aqueous and organic solvents (methanol, acetone and water). The antimicrobial activity of the concentrated extracts was evaluated by determination of the diameter of zone of inhibition against both gram negative and gram positive bacteria and fungi using the paper disc diffusion method. The least activity was shown by aqueous extract against E. coli (6.5 mm) and Bacillus subtilis (10 mm) while the maximum was recognized by the alcoholic extract, with a recorded zone diameter for E. coli (14 mm) and Bacillus subtilis (17 mm). Phytochemical screening of the plant exposed the presence of tannins, saponins and cardiac glycosides. The results of this analysis support the traditional use of Eucalyptus leaves as an antibacterial agent.

EVALUATION OF ANTIMICROBIAL ACTIVITY AGAINST GRAM POSITIVE AND GRAM NEGATIVE BACTERIA IN BARK EXTRACT OF ACACIA NILOTICA L

IJARW, 2021

In the present investigation the antimicrobial activity of Acacia nilotica L. in ethanolic and distilled water bark extract against gram-negative bacteria(Escherichia coli and Klebsiella pneumoniae and gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis) was evaluated for different polluted areas and was compared by reference area of Indore city. Investigation showed significant activity in ethanolic bark extracts against Escherichia coli, Staphylococcus aureus, and Bacillus subtilis in comparison to distilled water. The maximum zone of inhibition (mm) was found to be at VPA followed by MPA and LPA. In IPA reduced trend was seen in both the extracts.