Histological Evaluation of Guided Bone Regeneration in Osseous Defects Using A Novel Non-Resorbable Membrane (original) (raw)
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Guided bone regeneration using resorbable and non-resorbable membranes: a histological study in dogs
Odonto-stomatologie tropicale = Tropical dental journal, 2012
The aim of this study was to evaluate the effectiveness of e-PTFE titanium reinforced and Guidor membranes in the management of alveolar ridge deformities. Five beagle dogs were included in the study. Three through and through defects of the same size (12 x 5 x 5) were created in each animal, one on the right side and two defects 3-4 mm distant from each other on the left side. Guidor membrane was applied to the right defect, e-PTFE was applied to one of the left defects while the third defect served as control. Three, six and nine months following membrane placement, the dogs were euthanized and membranes with surrounding bone tissues harvested for histological analysis. Healing in each animal was uneventful with no clinical sign of inflammation. Histological evaluation indicated the defects covered with both membranes had significantly gained greater bone formation than the control defects. On the other hand, the control defects healed thin rim of bone mostly with a persistent cen...
Guided bone regeneration with a synthetic biodegradable membrane: a comparative study in dogs
Clinical Oral Implants Research, 2011
Objectives: The aim of the present study was to compare a newly developed biodegradable polylactide/polyglycolide/N-methyl-2-pyrrolidone (PLGA/NMP) membrane with a standard resorbable collagen membrane (RCM) in combination with and without the use of a bone substitute material (deproteinized bovine bone mineral [DBBM]) looking at the proposed tenting effect and bone regeneration. Materials and methods: In five adult German sheepdogs, the mandibular premolars P2, P3, P4, and the molar M1 were bilaterally extracted creating two bony defects on each site. A total of 20 dental implants were inserted and allocated to four different treatment modalities within each dog: PLGA/NMP membrane only (Test 1), PLGA/NMP membrane with DBBM (Test 2), RCM only (negative control), and RCM with DBBM (positive control). A histomorphometric analysis was performed 12 weeks after implantation. For statistical analysis, a Friedman test and subsequently a Wilcoxon signed ranks test were applied. Results: In four out of five PLGA/NMP membrane-treated defects, the membranes had broken into pieces without the support of DBBM. This led to a worse outcome than in the RCM group. In combination with DBBM, both membranes revealed similar amounts of area of bone regeneration and bone-to-implant contact without significant differences. On the level of the third implant thread, the PLGA/NMP membrane induced more horizontal bone formation beyond the graft than the RCM. Conclusion: The newly developed PLGA/NMP membrane performs equally well as the RCM when applied in combination with DBBM. Without bone substitute material, the PLGA/NMP membrane performed worse than the RCM in challenging defects, and therefore, a combination with a bone substitute material is recommended. To cite this article: Jung RE,
BMC Medicine, 2012
Treatment of large bone defects represents a great challenge in orthopedic and craniomaxillofacial surgery. Although there are several methods for bone reconstruction, they all have specific indications and limitations. The concept of using barrier membranes for restoration of bone defects has been developed in an effort to simplify their treatment by offering a sinlge-staged procedure. Research on this field of bone regeneration is ongoing, with evidence being mainly attained from preclinical studies. The purpose of this review is to summarize the current experimental and clinical evidence on the use of barrier membranes for restoration of bone defects in maxillofacial and orthopedic surgery. Although there are a few promising preliminary human studies, before clinical applications can be recommended, future research should aim to establish the 'ideal' barrier membrane and delineate the need for additional bone grafting materials aiming to 'mimic' or even accelerate the normal process of bone formation. Reproducible results and long-term observations with barrier membranes in animal studies, and particularly in large animal models, are required as well as well-designed clinical studies to evaluate their safety, efficacy and cost-effectiveness.
The effect of different membranes on onlay bone graft success in the dog mandible
Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology, 1998
The aim of this study was to compare resorbable membranes, nonresorbable membranes, and no coverage with respect to onlay bone graft healing in a dog. Experimental design. Each of four dogs had six corticocancellous blocks of ramus bone placed bilaterally as onlay grafts to the inferior lateral aspect of the mandible. On each side, one graft was left uncovered, one was covered with a collagen membrane, and one was covered with an expanded polytetrafluoroethylene membrane. Volume determinations were made at the time of graft placement and when the animals were killed.
Clinical Oral Implants Research, 2008
Objectives: The aim of the present study was to evaluate immunohistochemically the pattern of guided bone regeneration (GBR) using different types of barrier membranes. Material and methods: Standardized buccal dehiscence defects were surgically created following implant bed preparation in 12 beagle dogs. Defects were randomly assigned to six different GBR procedures: a collagen-coated bone grafting material (BOC) in combination with either a native, three cross-linked, a titanium-reinforced collagen membrane, or expanded polytetrafluorethylene (ePTFE), or BOC alone. After 1, 2, 4, 6, 9, and 12 weeks of submerged healing, dissected blocks were processed for immunohistochemical (osteocalcin-OC, transglutaminase II-angiogenesis) and histomorphometrical analysis [e.g., bone-to-implant contact (BIC), area of new bone fill (BF)]. Results: In general, angiogenesis, OC antigen reactivity, and new bone formation mainly arose from open bone marrow spaces at the bottom of the defect and invaded the dehiscence areas along the implant surface and BOC. At 4 weeks, membranes supporting an early transmembraneous angiogenesis also exhibited some localized peripheral areas of new bone formation. However, significantly increasing BIC and BF values over time were observed in all groups. Membrane exposure after 10-12 weeks was associated with a loss of the supporting alveolar bone in the ePTFE group. Conclusion: Within the limits of the present study, it was concluded that (i) angiogenesis plays a crucial role in GBR and (ii) all membranes investigated supported bone regeneration on an equivalent level.
Biomaterials, 1996
The aim of this study was an evaluation of the possibility of bone regeneration in connection with implant placement, using a new bioresorbable membrane (Guidor Matrix Barrier@) used previously in periodontal tissues regeneration. The study compared the bone regeneration obtained around Bonefit-ITI implants inserted in rabbit tibia using Guidor membranes, Gore-Tex@ membranes and in control sites. Microscopic analysis was performed after 6, 9 and 12 weeks. It was possible to see that the amount of bone around implants covered by Guidor and Gore-Tex membranes was roughly equivalent in all experimental sites at the 6-, 9-and IBweek time intervals. Also, all control sites healed completely. It could be concluded from this study that: (1) rabbit tibia cannot be recommended in research connected with guided bone regeneration, as all control sites healed in the same way as the test sites; (2) in no case did the presence or the degradation of the resorbable membrane prevent the formation of new bone; (3) no inflammatory reaction was present around the bioresorbable and the non-resorbable membranes; (4) bone formed in all cases on the outer surface of the non-resorbable membranes; (5) large Guidor membrane fragments were present in the g-week specimens, while in the 1Sweek specimens only small fragments were recognizable; (6) Guidor membranes can be used in guided bone regeneration.
Biomaterials, 2002
Two main types of membrane barriers are used for bone regeneration, non-resorbable and resorbable. Polytetrafluorethilene nonresorbable membranes have been extensively studied but they require a second surgical step for removal. Although polylactic acid (PLA) resorbable membranes avoid this problem, they have not been sufficiently evaluated on bone defects. The purpose of this pilot study was to compare the healing events of bone regeneration after placement of non-resorbable or resorbable membranes and to evaluate the amount of newly formed bone 2 and 4 months after membrane placement. Mandibular second, third and fourth premolars of four adult mongrel dogs were extracted bilaterally. Two rectangular bone defects (8 mm corono-apical and 12 mm mesial-distal) were created bilaterally 3 months after tooth extractions. Each dog received two resorbable membranes and one nonresorbable membrane; one defect was left untreated. Two dogs were killed at 2 months and the remaining two at 4 months following surgery. Undecalcified sections were obtained and stained with toluidine blue and pyronin G. Histomorphometric analysis was performed using the NIH Image software. Newly formed bone was observed under both resorbable and non-resorbable membranes. The amount of regenerated bone was similar between both treatments at 2 and 4 months after surgery. At 2 months, the newly formed bone was still immature whereas at 4 months some areas of woven bone were observed. The bone formation observed in the untreated defects was significantly lower than that observed in both resorbable and non-resorbable membrane-protected defects. In summary, the present study suggests that PLA membranes can yield good results when used on bone defects while avoid a second surgical procedure. r
Development of a New Barrier Membrane for Guided Bone Regeneration: an in Vitro and in Vivo Study
Journal of Oral Tissue …, 2011
Obtaining better outcomes from guided bone regeneration techniques often requires the use of ideal membranes. The objective of this study was to develop a novel membrane and investigate its contribution to the enhancement of bone regeneration. The membrane was fabricated by combining β-TCP particles with dissolved gelatin hydrogel and cross-linking molecules with glutaraldehyde. Rat bone-marrow cultured on this gelatin membrane for 14 days revealed time-dependent cell proliferation and the presence of alkalinephosphatase-positive cells as well as that on a collagen membrane. Histological analysis indicated that significantly more new bone volume was generated in symmetrical full-thickness bone defects of rat calvariae covered by the gelatin membrane compared to that of the uncovered control defects at 4 and 8 weeks post operation. The present study demonstrates that the novel β-TCP-containing gelatin hydrogel membrane stimulates bone regeneration. These results suggest the feasibility of this novel membrane for use in guided bone regeneration.