Guided bone regeneration using resorbable and non-resorbable membranes: a histological study in dogs (original) (raw)
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Biomaterials, 2002
Two main types of membrane barriers are used for bone regeneration, non-resorbable and resorbable. Polytetrafluorethilene nonresorbable membranes have been extensively studied but they require a second surgical step for removal. Although polylactic acid (PLA) resorbable membranes avoid this problem, they have not been sufficiently evaluated on bone defects. The purpose of this pilot study was to compare the healing events of bone regeneration after placement of non-resorbable or resorbable membranes and to evaluate the amount of newly formed bone 2 and 4 months after membrane placement. Mandibular second, third and fourth premolars of four adult mongrel dogs were extracted bilaterally. Two rectangular bone defects (8 mm corono-apical and 12 mm mesial-distal) were created bilaterally 3 months after tooth extractions. Each dog received two resorbable membranes and one nonresorbable membrane; one defect was left untreated. Two dogs were killed at 2 months and the remaining two at 4 months following surgery. Undecalcified sections were obtained and stained with toluidine blue and pyronin G. Histomorphometric analysis was performed using the NIH Image software. Newly formed bone was observed under both resorbable and non-resorbable membranes. The amount of regenerated bone was similar between both treatments at 2 and 4 months after surgery. At 2 months, the newly formed bone was still immature whereas at 4 months some areas of woven bone were observed. The bone formation observed in the untreated defects was significantly lower than that observed in both resorbable and non-resorbable membrane-protected defects. In summary, the present study suggests that PLA membranes can yield good results when used on bone defects while avoid a second surgical procedure. r
Brazilian Journal of Oral Sciences, 2013
To investigate the amount of connective tissue migrated into the extraction socket using E-PTFE and latex membranes. Methods: Seventeen rats were selected and randomly divided into 3 groups: e-PTFE membrane (n = 6), Latex membrane (n = 6) and Control (no membrane, n=5). After extraction of the maxillary right incisor, the animals of the test groups were subjected to alveolar guided bone regeneration (GBR) surgery and received an expanded polytetrafluoroethylene (e-PTFE) and a latex membrane, respectively. Thirty days after surgery, the animals were killed and histometric analysis was done to evaluate the migration of connective tissue. Data were analyzed statistically by one-way ANOVA and multiple-comparison Tukey's test at 5% significance level. Results: There was statistically significant difference between groups e-PTFE and Latex (p=0.001), and between groups e-PTFE and Control (p=0.012), but no significant difference was found between groups Latex and Control (p=0.416). Conclusions: The e-PTFE membrane showed better results and appeared more adequate for GBR therapy, forming a barrier to prevent the migration of connective tissue into the extraction socket. The latex membrane, on the other hand, did not show benefits over the control group.
The International journal of oral & maxillofacial implants
This prospective case series evaluated the use of a new titanium-reinforced nonresorbable membrane (high-density polytetrafluoroethylene), in combination with a mixture of anorganic bovine bone-derived mineral (ABBM) and autogenous particulated bone, for vertical augmentation of deficient alveolar ridges. A mixture of ABBM and autogenous particulated bone was used for vertical ridge augmentation and covered with a new titanium-reinforced nonresorbable membrane. Ridge measurements were obtained before and after the procedure, complications were recorded, and biopsy specimens were taken for histologic examination. Twenty vertical ridge augmentation procedures were carried out in 19 patients. All treated defect sites exhibited excellent bone formation, with an average bone gain of 5.45 mm (standard deviation 1.93 mm). The healing period was uneventful, and no complications were observed. Eight specimens were examined histologically; on average, autogenous or regenerated bone represente...
Acta Veterinaria, 2020
Post extraction alveolar ridge preservation is a preventive oral surgical procedure aimed to preserve the dimensions of the alveolar ridge after removal of the teeth. According to literature data, the utilization of guided bone regeneration for this purpose is absolutely justified. The aim of this study was to examine the effect of resorbable collagen membranes placed in two layers in respect to the application of membranes placed in one layer to the degree of bone regeneration after a tooth extraction. This experimental study was conducted on six adult dogs. In the first phase all four premolars were extracted on both sides in the lower jaw. After the volume-standardisation with bone drill, three post extraction alveoli were filled with bone substitute of equine origin and then covered with: collagen bioresor bable membrane of porcine origin; collagen bioresorbable membrane of equine origin; pericardial bioresorbable membrane of equine origin. The membranes on the left side were pl...
Open Access Macedonian Journal of Medical Sciences
BACKGROUND: Guided bone regeneration and guided tissue regeneration procedures have been performed using barrier membranes in clinical dentistry to enhance bone formation in osseous defects, ridge preservation and reconstruction. Despite the considerable number of new biomaterials that develop with more advantages and fewer disadvantages for bone regeneration, the cost and surgical approach difficulties are still the main obstacles that we tried to overcome using the novel membrane. AIM: Our research aimed to assess histologically the bone formation using the novel membrane in experimental bone defects. MATERIAL AND METHODS: Our study was conducted on ten mongrel dogs. Each animal presented two groups. The first group was at the left side of the animal mandible which received Bio-Oss only while the right side received Bio-Oss and was covered by the novel non-resorbable membrane. These dogs were sacrificed (3, 6, 9 and 12 weeks) postoperatively for histological assessment of healing ...
Clinical Oral Implants Research, 2001
Lateral ridge augmentation has become a standard treatment option to enhance the bone volume of deficient recipient sites prior to implant placement. In order to avoid harvesting an autograft and thereby eliminating additional surgical procedures and risks, bone grafting materials and substitutes are alternative filler materials to be used for ridge augmentation. Before clinical recommendations can be made, such materials must be extensively studied in experimental models simulating relevant clinical situations. The present pilot study was conducted in three dogs. Different grafting procedures were evaluated for augmentation of lateral, extended (8¿10¿14 mm) and chronic bone defects in the mandibular alveolar ridge. Experimental sites received tricalcium phosphate (TCP) granules or demineralized freeze-dried bone allograft (DFDBA) particles. Barrier membranes (ePTFE) were placed for graft protection. These approaches were compared to ridge augmentation using autogenous corticocancellous block grafts, either with or without ePTFE-membrane application. After a healing period of six months, the sites were analyzed histologically and histomorphometrically. Autografted sites with membrane protection showed excellent healing results with a well-preserved ridge profile, whereas non-protected block grafts underwent bucco-crestal resorption, clearly limiting the treatment outcome. The tested alloplastic (TCP) and allogenic (DFDBA) filler materials presented inconsistent findings with sometimes encapsulation of particles in connective tissue, thereby reducing the crestal bone width. The present pilot study supports the use of autografts with barrier membranes for lateral ridge augmentation of extended alveolar bone defects.
Guided bone regeneration with a synthetic biodegradable membrane: a comparative study in dogs
Clinical Oral Implants Research, 2011
Objectives: The aim of the present study was to compare a newly developed biodegradable polylactide/polyglycolide/N-methyl-2-pyrrolidone (PLGA/NMP) membrane with a standard resorbable collagen membrane (RCM) in combination with and without the use of a bone substitute material (deproteinized bovine bone mineral [DBBM]) looking at the proposed tenting effect and bone regeneration. Materials and methods: In five adult German sheepdogs, the mandibular premolars P2, P3, P4, and the molar M1 were bilaterally extracted creating two bony defects on each site. A total of 20 dental implants were inserted and allocated to four different treatment modalities within each dog: PLGA/NMP membrane only (Test 1), PLGA/NMP membrane with DBBM (Test 2), RCM only (negative control), and RCM with DBBM (positive control). A histomorphometric analysis was performed 12 weeks after implantation. For statistical analysis, a Friedman test and subsequently a Wilcoxon signed ranks test were applied. Results: In four out of five PLGA/NMP membrane-treated defects, the membranes had broken into pieces without the support of DBBM. This led to a worse outcome than in the RCM group. In combination with DBBM, both membranes revealed similar amounts of area of bone regeneration and bone-to-implant contact without significant differences. On the level of the third implant thread, the PLGA/NMP membrane induced more horizontal bone formation beyond the graft than the RCM. Conclusion: The newly developed PLGA/NMP membrane performs equally well as the RCM when applied in combination with DBBM. Without bone substitute material, the PLGA/NMP membrane performed worse than the RCM in challenging defects, and therefore, a combination with a bone substitute material is recommended. To cite this article: Jung RE,
Journal of Biomedical Materials Research Part B: Applied Biomaterials, 2009
Aim: This study aimed to evaluate clinical outcomes of titanium membrane and compare these findings with clinical outcomes of e-PTFE membrane, and to investigate the effect of bacterial contamination on both membranes with SEM during long-term healing. Results: Sixteen titanium and sixteen e-PTFE membranes were surgically placed adjacent to periodontally involved teeth. Seven titanium and 8 e-PTFE membranes were exposed between 4 and 6 weeks. There were no significant difference between groups for plaque and gingival index. Probing depth and clinical attachment level (CAL) were decreased in both groups when compared with baseline; however, these differences were not statistically significant. The CAL gains between the groups were statistically different in 3rd, 6th, 9th, 12th, and 24th months (p < 0.05), and the CAL gain was significantly higher in titanium membrane (p < 0.05). There was significant decrease in bleeding on probing from baseline in both groups (p < 0.05). Surfaces of 15 membranes were studied using SEM. The largest amount of bacteria was found on the external cervical surfaces of 15 exposed specimens. The entire surface showed the presence of slough epithelial cells, leukocytes, red blood cells, yeast, and microbial plaque. Thirteen external mid surfaces of the 15 specimens, external apical surfaces of three e-PTFE and 1 titanium membrane, internal collar surfaces of all specimens, internal mid surfaces of 5 e-PTFE and three titanium membranes and internal apical surface of only one e-PTFE membrane were infected. Conclusions: This study demonstrated that titanium membrane is equivalent to e-PTFE membranes for GTR in the treatment of periodontal defects. ' 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 91B: 772-779, 2009
Materials
Aim: The aim of the present study was to investigate quantitative histological examination of bone reconstructed with non-resorbable high-density polytetrafluoroethylene membrane (d-PTFE), left intentionally exposed in post extraction sockets grafted with anorganic bone material, and removed after four weeks, versus extraction and guided bone regeneration (GBR), performed two months later. Materials and Methods: This study was designed as a multicenter randomized controlled trial of parallel-group design. Patients were selected and consecutively treated in three centers in Italy. Patients randomly received intentionally exposed non-resorbable d-PTFE membrane (group A), or guided bone regeneration (group B), to treat post-extractive alveolar bone defects with implant-supported restorations. Outcomes were: the implant failure, any mechanical and biological complications, patient satisfaction, and qualitative and histomorphometric evaluation of the collected bone samples. Results: Eigh...