The genetic diversity and population structure of common bean (Phaseolus vulgaris L) germplasm in Uganda (original) (raw)
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Genetic diversity of common bean accessions in the germplasm bank of the Instituto Agronômico–IAC
2005
The genetic divergence among 993 accessions of common bean (Phaseolus vulgaris L.) in the germplasm bank of the Instituto Agronômico IAC (agronomical Institute in Campinas, state of São Paulo) was evaluated in an analysis using 23 qualitative and quantitative agromorphological/ morphological and agronomic descriptors. Multivariate analyses such as the Euclidean distance, principal component analysis and Tocher's cluster method were used to quantify the divergence among the accessions. The joint use of the analyses allowed the identification of 45 groups of which the first five absorbed 88.28% of all accessions. Among the 23 evaluated descriptors, the principal component analysis allowed the discarding of five that were redundant and/or little variable and the eight first principal components were necessary to explain 70% of the total variation. The multivariate methods proved efficient when used in germplasm collections, optimizing the descriptor set and establishing an orientation for future crossings in the common bean improvement program of the IAC.
Plant Genetic Resources: Characterization and Utilization
Germplasm of common beans from the Mesoamerican gene pool races: Durango, Jalisco, Mesoamerica and Guatemala have highest genetic variation for the crop's improvement. The objective was to assign 50 common bean germplasm in Uganda into its gene pool races based on analyses of population structure. Secondly, to estimate heritability and effects of genotype × environment (GXE) interaction on common bean agronomic and yield traits in space and time. Sample genomic DNA was amplified in 2011 with 22 Simple sequence repeat markers (SSRs) and alleles separated using capillary electrophoresis. Field evaluations were conducted in 2010 and 2011 at NaCRRI and 2015 at CIAT – Kawanda. Multivariate analyses of SSRs data identified four subgroups within the germplasm: K4.1–K4.4, with corresponding Wrights fixation indices (FST) as 0.1829 for K4.1, 0.1585 for K4.4, 0.1579 for K4.2 and least for K4.3 at 0.0678. Gene pool race admixtures in the population (14%) were notable and attributed to gene...
Indian Journal Of Agricultural Research, 2020
Common bean (Phaseolus vulgaris L.) is native to Middle America and Andean mountains of South America. Due to its nutritious value, it is one of the most important legume crop of the world. Evaluation of genetic diversity of a crop is extremely important for the establishment of a breeding program and selection of varieties for cultivation. We evaluated the genetic diversity and phylogenetic relationship of 40 P. vulgaris ecotypes from CIAT (International Center for Tropical Agriculture) germplasm using seed protein profiles produced by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), to screen the germplasm to be used in a breeding program. A total of 21 bands scored 14 were found polymorphic. A distance matrix was generated from the similarity matrix based on polymorphic bands. The UPGMA tree was established through cluster analysis performed on the distance matrix. Six major clusters were formed on the basis of SDS_PAGE analysis with one ecotype (E13-G16832)...
IRAQI JOURNAL OF AGRICULTURAL SCIENCES
The objectives of this study were to estimate the performance of the common bean (Phaseolus vulgaris L.) genotypes under water-stress conditions, and their genetic diversity. White bean surpassed the others for relative water content, root/shoot ratio and leaf area under water-stress condition. Scatter plot indicates a strong association of yield with pod numbers plant-1, branch number and harvest index. A total of 69 polymorphic were obtained, applying 26 SSR primers on 14 genotypes. Major allele frequency was 0.601, and the average value of PIC was 0.407. The highest value of gene diversity (0.745) and PIC (0.704) were recorded for BMd-23 marker. Molecular variance among population indicated 25%, while 47% was realized within populations. Structure analysis divided the common bean genotypes into three groups (DeltaK value =3). Chity and Boschbohnen were identified to have a mixed ancestor while all the others were pure at their populations. A dendrogram and PCoA analyses are acco...
Scientia Horticulturae, 2021
Genetic diversity and structure of common bean (Phaseolus vulgaris) germplasm from Serbia, comprising 118 landraces and 18 cultivars, was assessed with the application of 27 Single Sequence Repeats (SSR) markers. Thirteen accessions from Agricultural Institute of Slovenia were used as references for gene pool determination. Main parameters of genetic diversity were calculated for each SSR loci, i.e. number of different and rare alleles, number of effective alleles, Shannon's information index, observed and expected heterozygosity and polymorphic information content. A total of 445 allelic variants, with 16.5 alleles per locus on average, were detected. Mean gene diversity (He= 0.79) indicated sufficient reservoir of genetic variation preserved in studied bean germplasm. Landraces displayed higher variability compared to cultivars (405 in relation to 233 allelic variants). Genetic 2 structure and relatedness of accessions was assessed by model-based method and hierarchical clustering method in combination with genetic distance calculation. The Bayesian clustering model implemented in STRUCTURE software, on the primary level (K=2), revealed clear separation of accessions into two groups, corresponding to gene pool affiliation. Mesoamerican gene pool (M) was represented with 23.5% of accessions, while Andean (A) was larger, composed of 68.4% of studied germplasm. Small group (8.1%) showed admixed genetic structure between two gene pools. Additional variation in respect to two recognized gene pools was revealed (K=3), whose basis was acknowledged to be within Andean gene pool. Further subdivision of accessions (K=8), mainly according to the seed forms, was observed. Genetic distance analysis associated with Neighbour-joining clustering method revealed grouping pattern of landraces and cultivars corresponding to the gene pool and their seed phenotypes. Classification and structuring of the bean accessions according to and beyond the gene pool of origin should facilitate conservation strategies and breeding of this material. Combining the information of phenotypic variation obtained in previous research and molecular data reveled in this study will assist in selection of parental components for breeding, or in the choice of smaller sample in order to further acknowledge their breeding value. In addition, obtained results of this work should serve as an additional information on common bean germplasm variation in Western Balkans and beyond, in Europe.
Advances in agriculture, 2023
Twenty-fve common bean genotypes were evaluated to assess the genetic variability, trait association, and determine the direct and indirect efects of traits on seed yield. Te genotypes were grown in a lattice design at the research farm of the Melkassa Agricultural Research Center, Melkassa and Miesso, in the 2018 cropping season. Analysis of variance revealed that signifcant diferences were observed among the genotypes at individual locations. Te phenotypic coefcient of variation values were moderate for number of nodes, seeds per pod, plant height, and hundred seed weight at Melkassa. Moderate genotypic coefcient of variation values were obtained for pods per plant and seeds per pod. At Miesso, moderate phenotypic coefcients of variation values were recorded for number of nodes, hundred seed weight, and plant height. High heritability estimates were obtained for seed yield and hundred seed weight at Melkassa and for seed yield at Miesso, indicating that selection could be fairly easy and improvement is possible using these traits in a common bean breeding program. High genetic advances were obtained for seed yield and pods per plant at Melkassa, while moderate genetic advances as a percent of the mean were attained for plant height, hundred seed weight, and seeds per pod. Similarly, at Miesso, high and moderate genetic advances as percent of mean values were obtained for seed yield and hundred seed weight, respectively. Seed yield showed positive and signifcant phenotypic association with days to fowering, internode length, and pods per plant at Melkassa and had positive and signifcant phenotypic association with seeds per pod and hundred seed weight at Miesso. Hence, selection of those genotypes based on the traits with high genotypic coefcient of variability, heritability, genetic advance, and positive correlation coefcient and direct efect on seed yield can be recommended for further yield improvement at the respective location and at the national level in general.
Genetics and Molecular Research, 2011
Dry beans are considered to be a crop of great socioeconomic importance, because they are an inexpensive source of nutrients and because their cultivation requires considerable manual labor. Studies of genetic diversity have been very important for genetic improvement programs, because they give parameters for the identification of genitors that can provide large heterosis effects and improved segregation in recombinants, increasing the probability of obtaining superior genotypes in the progeny. We evaluated the genetic diversity of 57 dry bean accessions, including 31 local accessions, propagated by small-scale farmers, 20 accessions supplied by the Brazilian Agricultural Research Agency, and six commercial accessions, using 16 microsatellite primers. Among these primers, 13 were found to be polymorphic, giving 29 polymorphic alleles. The largest number of alleles per locus was observed for primer BM141, which had four alleles. The polymorphic information content varied from 0.11 to 0.51, observed for loci BM212 and BM141, respectively. The lowest degree ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 10 (1): 140-149 (2011) Genetic diversity in common bean of dissimilarity (0.0) was found between the accession Iapar 81 and the accessions E03, E04, E09, and E13 and between the accession pairs E08 with E16 and Iapar 31 with E06. The highest degree of dissimilarity was found between the accessions Carioca and E22 (1.0). Grouping analysis revealed four groups, according to the place of origin. This tendency was also found in the principal coordinate analysis. The local genotypes were found to have relatively high genetic diversity, while the EMBRAPA and commercial cultivars had a relatively narrow genetic basis.