Identification and Broad Dissemination of the CTX-M-14 Lactamase in Different Escherichia coli Strains in the Northwest Area of Spain (original) (raw)
Related papers
Journal of Clinical Microbiology, 2002
During the course of a molecular epidemiology study of mechanisms of antibiotic resistance in the area served by our hospital (516,000 inhabitants), we isolated the gene encoding CTX-M-14 β-lactamase. Thirty clinical strains (27 Escherichia coli and 3 Klebsiella pneumoniae isolates) with a phenotype of extended-spectrum β-lactamase were collected from January to October 2001 and studied for the presence of the CTX-M-14 β- lactamase gene. By isoelectric point determination, PCR, and nucleotide sequencing, we detected the presence of this gene in 17 E. coli strains belonging to 15 different genotypes (REP-PCR) causing infections in 17 different patients. Epidemiological studies based on medical records did not suggest any relationship between the patients infected with these E. coli strains and, interestingly, 7 of 30 patients harboring strains with extended-spectrum β-lactamases never had contact with the hospital environment before the clinical E. coli isolation. Conjugation experim...
Emerging Infectious …, 2008
We analyzed 43 CTX-M-15–producing Escherichia coli isolates and 6 plasmids encoding the blaCTX-M-15 gene from Canada, India, Kuwait, France, Switzerland, Portugal, and Spain. Most isolates belonged to phylogroups B2 (50%) and D (25%). An EC-B2 strain of clonal complex sequence type (ST) 131 was detected in all countries; other B2 isolates corresponded to ST28, ST405, ST354, and ST695 from specific areas. EC-D strains were clonally unrelated but isolates from 3 countries belonged to ST405. All CTX-M-15 plasmids corresponded to IncFII group with overrepresentation of 3 HpaI-digested plasmid DNA profiles (A, B and C; 85–120kb, similarity >70%). Plasmid A was detected in EC-B2 strains (ST131, ST354, or ST405), plasmid C was detected in B2 and D strains, and plasmid B was confined to worldwide-disseminated ST131. Most plasmids contained blaOXA-1, aac(6′)-Ib-cr, and blaTEM-1. Worldwide dissemination of CTX-M-15 seems to be determined by clonal complexes ST131 and ST405 and multidrug-resistant IncFII plasmids.
Antimicrobial Agents and Chemotherapy, 2006
One hundred twenty CTX-M-15-producing Escherichia coli strains isolated in 10 different hospitals from Paris (France), in the Hospital Charles Nicolle in Tunis (Tunisia), and in the Pasteur Institute in Bangui, Central African Republic (CAR), between 2000 and 2004 were studied. Eighty isolates, recovered from the three countries, were clonally related by repetitive extragenic palindromic PCR and pulsed-field gel electrophoresis. Various resistance profiles were identified among these clonal strains. After conjugation or electroporation of plasmids from E. coli strains representative of each profile and each geographic region, we observed seven resistance profiles in the recipient strains. Incompatibility typing showed that all the plasmids transferred from the clonal strains studied, except one, belonged to the incompatibility group FII. They all shared a multidrug resistance region (MDR) resembling the MDR region located in pC15-1a, a plasmid associated with an outbreak of a CTX-M-...
Dissemination of CTX-M-Type Extended-Spectrum -Lactamase Genes to Unusual Hosts
Journal of Clinical Microbiology, 2005
A Citrobacter amalonaticus and a Morganella morganii producing the CTX-M-1 extended-spectrum -lactamase (ESBL) were isolated from an area where this enzyme is now widespread in Escherichia coli. This is the first report of CTX-M-1 in the former species. In both cases the ESBL determinant was possibly acquired by these unusual hosts in vivo, after coinfection with E. coli strains carrying conjugative plasmids encoding CTX-M-1.
Journal of global antimicrobial resistance, 2015
Escherichia coli producing extended-spectrum b-lactamase (ESBL) of the CTX-M type is among the fastest growing resistance problems both in community-and hospital-acquired infections worldwide. Zoonotic transmission of CTX-M-1 has been suggested based on the relative frequency of this CTX-M type among E. coli isolates of animal origin. A Dutch study showed some degree of genetic relatedness between ESBLproducing E. coli isolates from patients, poultry and retail chicken meat, and detected bla CTX-M-1 on the same plasmid incompatibility group (IncI1) among human and animal isolates [1]. In contrast, a similar study from the UK found no association between CTX-M-producing E. coli isolated from poultry and human patients, clearly indicating geographical variations in the epidemiology of CTX-M, even between closely located countries [2]. In Denmark, a nationwide prevalence study of ESBL-producing E. coli in urine and blood from hospitals and the community conducted in 2007 showed that CTX-M-15 was