The Effect of Oral Administration of Hypericum Perforatum on Serum Glucose and Lipids, Hepatic Enzymes and Lipid Peroxidation in Streptozotocin-Induced Diabetic Rats (original) (raw)
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Anti-diabetic activity of Indian Hypericum perforatum L. on alloxan-induced diabetic rats
2008
Anti-diabetic activity of the standardised extract of Indian Hypericum perforatum L. was investigated on alloxan induced diabetic rats. Indian Hypericum perforatum (IHp) extract was orally administered (100 and 200 mg/kg) as suspension in 0.3 % carboxy methyl cellulose for 14 days. Glibenclamide (10 mg/kg/day, p.o.) was used as standard. Blood samples were collected at day 0 and 14 th day (1 hour after last dose) from orbital sinus and blood glucose was estimated by commercially available kit. IHp treatment led to significant fall (p<0.01) in elevated blood glucose level. Moreover, IHp treatment also reverses the weight loss associated with alloxan treatment. The overall results indicate that IHp possess significant antidiabetic activity.
Despite orthodox modalities, there is still a high demand for herbal preparations with anti-diabetic properties all over the world. The effects of many plants on glucose metabolism and hypoglycemia properties have been investigated. Aim: The histomorphological, hypoglycaemic and hepatoprotective effects of Persea Americana Seed Methanolic Extract in Alloxan-induced diabetic Wistar rats were investigated in this study. Methodology: Persea Americana seeds were collected, washed, air-dried, and ground into a fine powder and cold macerated in 0.5 L of water containing 80 percent v/v methanol for 72 hours at room temperature (26-28 0 C) and filtered with Whatman filter paper No.1. The filtrate was condensed to dryness in vapour at 40 0 C using water bath, yielding 9g (18% w/w) of a dark green semi-solid extract and stored in the refrigerator at 4 0 C until use. Thirty adult male Wistar rats, weighing 150-200g, were divided into six (6) groups, each with five rats (n=5). Diabetes was induced in rats using a single intraperitoneal injection of alloxan (150 mg/kg). Group 1 and 2 are the normal and diabetic control groups, respectively. In the diabetic group, groups 3, 4, and 5 were given 120, 240, and 480 mg/kg/day of Persea Americana Seeds extract, respectively, while Group 6 received 100 mg/kg/day of metformin for 14 days. The animals analysis. The liver tissues were also harvested for histomorphorlogical and biochemical evaluations. The SPSS package, version 23 was used to analyze data obtained and the results were expressed as Mean ± SEM. The results showed that in groups receiving metformin and seed extracts, elevated blood glucose levels were significantly lower than in control groups (P<0.05), with the 240 mg/kg dose having the most hypoglycaemic potential. Persea Americana seed extract inhibited alphaamylase and alpha-glucosidase enzymes and high antioxidant potential. The liver tissues of infected animals were also found to be recovering from the toxic effects of alloxan induction. The hypoglycaemic function of Persea Americana methanolic extract is mediated by enzyme inhibition and major antioxidant potentials, according to the report, which could enhance its anti-diabetic properties.
The insulin stimulative and antioxidative effect of Persea americana fruits extract were evaluated in streptozotocin (STZ). The ethanolic extract of P.americana at a concentration of 300mg/kg body weight/rat/day was orally administered to STZ-induced hyperglycemic rats for a period of 30 days. The elevated levels of blood glucose, glycosylated hemoglobin, blood urea, and serum creatinine in the hyperglycemic rats reverted back to near normal after treatment with the avocado fruit extract. Similarly significant decreases in the levels of plasma insulin and the hemoglobin were elevated to near normal after treatment with fruit extract, suggesting the insulin stimulative effect of P.americana fruit. Determination of thiobarbituric acid reactive substance (TBARS), hydroperoxides, and both enzymatic and nonenzymatic antioxidant evidenced the antioxidative potential of the extract of avocado fruit, which in turn may be responsible for its hypoglycemic potential. Alterations observed in the activities of pathophysiological enzymes such as serum aspartate transaminase (AST), serum alanine transaminase (ALT), and serum alkaline phosphatase (ALP) in the serum of control and experimental groups of rats revealed the tissue protective nature of Persea americana fruits in comparison with glyclazide, the standard reference drug.
Iranian Journal of Medical Sciences, 2020
Background: Heracleum persicum (H. persicum) is a medicinal herb used in Iranian traditional medicine for its anti-toxic property. It is commonly consumed in the form of food additives and as a medicinal herbal tonic to treat liver and kidney diseases. The present study aimed to investigate the anti-oxidant, anti-diabetic, and anti-hyperlipidemic effects of H. persicum hydroalcoholic extract in alloxan-induced diabetic rats. Methods: Adult male Wistar rats (n=30) were assigned to five groups: a normal group, a diabetic control group, and three diabetic groups treated orally with 200 and 400 mg/kg of the extract and 5 mg/kg of glibenclamide, respectively, for two weeks. Blood glucose and bodyweight were measured at the end of each week. On day 15, blood samples were collected to measure the levels of insulin, insulin growth factor-I (IGF-I), antioxidant markers for malondialdehyde (MDA), glutathione peroxidase (GPx), superoxide dismutase (SOD), total antioxidant activity (TAS), total...
Hypericum perforatum is known to have diverse medicinal uses for centuries. The antidepressant activity of Hypericum perforatum is widely accepted and proved in both animal and clinical studies. Present study was undertaken to investigate the effect of Hypericum perforatum in a battery of animal models for metabolic disorder. Hypericum is tested for hypolipidemic activity in normal rats, antiobesity activity in high-fat-diet induced obese rats, and fructose-fed rats. Hypericum was orally administered as suspension in 0.3% carboxymethyl cellulose at the doses of 100 and 200 mg/kg body weight for 15 consecutive days. Hypericum significantly lowered total cholesterol and low-density cholesterol in normal rats. Hypericum significantly inhibited weight gain in high-fat-fed rats. In fructose-fed rats, Hypericum normalised the dyslipidemia induced by fructose feeding and improved the insulin sensitivity. Taken together, Hypericum could be the antidepressant therapy of choice for patients suffering from comorbid diabetes and obesity.
African Journal of Traditional, Complementary and Alternative Medicines, 2011
Insulin-stimulative and anti-oxidative effects of Persea americana fruit extract were evaluated using streptozotocin (STZ). Ethanol extract of P. americana in the concentration of 300 mg/kg body weight/rat /day was orally administered to rats introduced into STZ-induced hyperglycaemic state for a period of 30 days. After the treatment with avocado fruit extract, the elevated levels of blood glucose, glycosylated haemoglobin, blood urea and serum creatinine seen in the hyperglycaemic rats, reverted back to near normal. Similarly, significantly decreased plasma insulin and haemoglobin levels went back to near normal after the treatment, suggesting the insulin-stimulative effect of P. americana fruit. Determination of thiobarbituric acid reactive substances (TBARS), hydroperoxides and both enzymatic and non-enzymatic antioxidants, confirmed the anti-oxidative potential of avocado fruit extract which, in turn, might be responsible for its hypoglycaemic potential. Changes in activities of enzymes such as serum aspartate transaminase (AST), serum alanine transaminase (ALT), and serum alkaline phosphatase (ALP) seen in the control and experimental rats, revealed the tissue-protective nature of Persea americana fruits, while all of the analysed biochemical parameters were comparable to those obtained with gliclazide as a standard reference drug.
ISRN …, 2011
Hypericum perforatum is known to have diverse medicinal uses for centuries. The antidepressant activity of Hypericum perforatum is widely accepted and proved in both animal and clinical studies. Present study was undertaken to investigate the effect of Hypericum perforatum in a battery of animal models for metabolic disorder. Hypericum is tested for hypolipidemic activity in normal rats, antiobesity activity in high-fat-diet induced obese rats, and fructose-fed rats. Hypericum was orally administered as suspension in 0.3% carboxymethyl cellulose at the doses of 100 and 200 mg/kg body weight for 15 consecutive days. Hypericum significantly lowered total cholesterol and low-density cholesterol in normal rats. Hypericum significantly inhibited weight gain in high-fat-fed rats. In fructose-fed rats, Hypericum normalised the dyslipidemia induced by fructose feeding and improved the insulin sensitivity. Taken together, Hypericum could be the antidepressant therapy of choice for patients suffering from comorbid diabetes and obesity.
Hypericum perforatum is known to have diverse medicinal uses for centuries. The antidepressant activity of Hypericum perforatum is widely accepted and proved in both animal and clinical studies. Present study was undertaken to investigate the effect of Hypericum perforatum in a battery of animal models for metabolic disorder. Hypericum is tested for hypolipidemic activity in normal rats, antiobesity activity in high-fat-diet induced obese rats, and fructose-fed rats. Hypericum was orally administered as suspension in 0.3% carboxymethyl cellulose at the doses of 100 and 200 mg/kg body weight for 15 consecutive days. Hypericum significantly lowered total cholesterol and low-density cholesterol in normal rats. Hypericum significantly inhibited weight gain in high-fat-fed rats. In fructose-fed rats, Hypericum normalised the dyslipidemia induced by fructose feeding and improved the insulin sensitivity. Taken together, Hypericum could be the antidepressant therapy of choice for patients suffering from comorbid diabetes and obesity.
African Journal of Pharmacy and Pharmacology, 2013
The leaves of Psidium guajava, Anacardium occidentale, Eucalyptus globulus and fruits of Xylopia aethiopica are used in the management of diabetes mellitus. Hence, the phytochemical constituents as well as the acute toxicity of the combined chloroform extracts (A. occidentale + E. globulus and P. guajava + X. aethiopica) and their effects (at graded doses of 100 and 250 mg/kg body weight each) on the concentration of malondialdahyde (MDA), activities of catalase (CAT), glutathione peroxidase (GPx) and superoxide dismutase (SOD) and concentration of vitamin C in diabetic and normal rats were investigated using standard methods. The phytochemical analyses of the four extracts showed the presence of terpenoids and fats and oil in all of them. Each of the combined extract was found to be non-toxic even at a dose as high as 5000 mg/kg body weight. The combined extracts at the tested doses significantly (p < 0.05) and dose-relatedly reduced the concentration of MDA, raised the activiti...