Comparative study of antidiabetic activity among three medicinal plants against streptozotocin induced diabetes rat model (original) (raw)
Related papers
2013
The leaves of Anchomanes difformis are used in traditional medicine to promote health in the ageing. This study investigated the antidiabetic activity of the ethanol extract of the leaves of Anchomanes difformis in albino rats. Diabetes was induced by intra-peritoneal injection of 160 mg/kg of alloxan monohydrate. Plant extract (250 mg/kg and 500 mg/kg) was administered orally and blood glucose levels were monitored at 2 hourly intervals and daily for 14 days. The LD50 was found to be greater than 5000 mg/kg. Phytochemical screening showed the presence of tannins, phlobatannins and alkaloids, flavonoids, reducing sugars, saponins and cardiac glycosides. The two doses of the extract produced a statistically significant reduction of fasting blood glucose levels during the acute study and prolonged treatment for 14 days when compared to glibenclamide and the negative controls. The plant extract seems to possess a dosedependent anti diabetic effect. Further studies will be done to evaluate the toxicological effects of this plant extract.
Antidiabetic Activity of Rotula aquatica Lour Roots in Streptozotocin Induced Diabetic Rats
2013
The study was designed to evaluate antidiabetic activity on methanolic extracts of Rotula aquatica Lour roots in Streptozotocin induced diabetic rats. The roots of Rotula aquatica Lour were extracted with methanol and evaluated for their antidiabetic activity in Streptozotocin induced diabetic rats at doses levels of 100, 200 and 400 mg/kg body weight. The lowering of blood sugar levels as well as percent blood glucose reduction were calculated and compared with the standard glibenclamide (0.45 mg/kg b.w.). Preliminary phytochemical screening was also conducted for occurrence of compounds. The oral administration of 400 mg/kg b.w. of methanolic extract of roots of Rotula aquatica showed more significant (P<0.01) decrease in blood glucose levels at 4 and 8 th hrs and highly significant (P<0.001) decrease in blood glucose levels at 8 th hr. The methanolic extracts of Rotula aquatica roots showed good activity at a dose of 400 mg/kg body weight .Phytochemical screening revealed p...
Many medicinal trees and plant extracts of bark, leaf and seeds are in use as antibacterial, hepatoprotective, haemostatic, astringent, anti-diarroheal and anti-inflammatory. Thespesia Populnea is known as Indian tulip tree and belongs to Malvaceae family of plant kingdom. It is distributed in coastal forest and tropical regions in India. On the other hand Helianthus annuus L., commonly known as sunflower, seeds are utilized for antidiabetic effect in Indian system of medicine. In the present review paper, both the plant extracts are compared and their uses were summarized. The ethanolic extract of the plant bark, leaf and ethanolic extract of sunflower seeds evaluation studies for their effect against the streptozotocin (STZ)–induced diabetic rats are discussed.
The study was designed to evaluate antidiabetic activity on methanolic extracts of Rotula aquatica Lour roots in Streptozotocin induced diabetic rats. The roots of Rotula aquatica Lour were extracted with methanol and evaluated for their antidiabetic activity in Streptozotocin induced diabetic rats at doses levels of 100, 200 and 400 mg/kg body weight. The lowering of blood sugar levels as well as percent blood glucose reduction were calculated and compared with the standard glibenclamide (0.45 mg/kg b.w.). Preliminary phytochemical screening was also conducted for occurrence of compounds. The oral administration of 400 mg/kg b.w. of methanolic extract of roots of Rotula aquatica showed more significant (P<0.01) decrease in blood glucose levels at 4 and 8 th hrs and highly significant (P<0.001) decrease in blood glucose levels at 8 th hr. The methanolic extracts of Rotula aquatica roots showed good activity at a dose of 400 mg/kg body weight .Phytochemical screening revealed presence of triterpenes and tannins in plant extracts.
ANTIDIABETIC POTENTIAL OF HERBAL EXTRACTS STUDIED IN (STZ) STREPTOZOTOCIN INDUCED DIABETIC RAT
Synthetic drugs are commonly used in the present day with the advancement in chemical technology awareness about its side effects is slowly triggering the use of non-synthetic curatives. The curative properties of these drugs are due to the complex chemical substances present as secondary plant metabolites in one or more parts of these plants. Diabetes Mellitus is a clinical condition characterized by increased blood glucose level due to the insufficient or inefficient insulin. Streptozotocin is to induce hyperglycemic condition. Gymnema sylvestre, Eugenia jambolana, Tulsi (Ocimum sanctum) and Bittergourd (Momordica charantia) are a popular remedy for the treatment of diabetes mellitus. In this study, oral administration of Gymnema sylvestre, Eugenia jambolana, Ocimum sanctum and Momordica charantia showed the hypoglycemic activity and it could exert a beneficial action against biochemical and histopathological alterations caused by streptozotocin. In the present study the antioxidative potential of Gymnema sylvestre, Eugenia jambolana, Ocimum sanctum and Momordica charantia was assessed in streptozotocin induced diabetic rats. Lipid peroxide levels were also measured in normal, diabetic and treated animals. Malondialdehyde (MDA) levels were significantly higher and antioxidant activity was found low in diabetic groups as compared to the control groups, and significant alteration in both the MDA levels and antioxidant activity was also observed when the above herbal hypoglycemic agents were given to diabetic rats. On the basis of our results we conclude that Gymnema sylvestre, Eugenia jambolana, Ocimum sanctum and Momordica charantia are not only useful in controlling the lipid peroxide levels but are also helpful in further strengthening the antioxidant potential.The probable mechanism by which Gymnema sylvestre, Eugenia jambolana, Ocimum sanctum and Momordica charantia exerts its protective action against streptozotocininduced pancreatic metabolic alterations could be by the stimulation of pancreatic regeneration through an improved synthesis of protein or accelerated detoxification and exertion. Furthermore, comprehensive chemical and pharmacological research is required to reveal the mechanism of the anti-diabetic potential of Gymnema sylvestre, Eugenia jambolana, Ocimum sanctum and Momordica charantia.
Ymer, 2022
Traditionally, Barleria buxifolia Linn is utilized for antidiabetic action with absence of logical investigation. Thus, the current examination was attempted to explore for its antidiabetic and antihyperlipidemic movement in streptozotocin instigated diabetic animal models. Blood glucose levels were estimated in normoglycemic rats at initial, 60 th and 120 th minutes intervals and in glucose feed hyperglycemic rats at initial, 30, 60, 90 and 120 minutes after solitary portion of streptozotocin at 55 mg/kg body weight intraperitoneal were made diabetic in albino rats. Blood glucose levels were estimated at week by week spans after every day administration of chloroform and methanol extracts of Barleria buxifolia at dosages of 250 and 500 mg/kg body weight. Other biochemical boundaries of serum triglycerides, cholesterol, HDL-cholesterol, LDL-cholesterol, VLDL-cholesterol, total protein, albumin, globulin, uric acid, creatinine, urea, transaminases, alkaline phosphatase, alanine aminotransaminase, insulin and glycosylated hemoglobin were likewise estimated toward the finish of the investigation. Chloroform and methanol extracts of Barleria buxifolia by oral organization for 21 days altogether (P<0.001) decreases the elevated blood glucose levels in diabetic rats whereas in normoglycemic rats it doesn't alter the blood glucose levels significantly and in glucose feed hyperglycemic rats significantly decreases the raised blood glucose levels. Likewise, the chloroform and methanol extracts of Barleria buxifolia improved other biochemical boundaries related with diabetes. Moreover, the extracts of Barleria buxifolia favorable affect the histopathological changes of pancreas in streptozotocin initiated diabetic rats. Delayed consequences legitimize the traditional utilization of Barleria buxifolia for its antidiabetic action.
Medicinal Plants: A Methodology for Studying their Anti-Diabetic Activity
TJPRC, 2013
This work is a review on the study methods of the antidiabetic activity of medicinal plants. Indeed, this discipline is not standardized and techniques used to assess this activity are very varied. The choices of the plant and the extract to be tested are major parameters before going to the model of diabetes. The latter is induced into animals by various techniques including the injection of chemicals such as streptozotocin which damages the pancreatic β cells. Hence, diabetes develops spontaneously in certain animal species. Works realized on animals (preclinical) would permit to research on human being (clinical).
Medicinal plants and diabetes mellitus: A review
Diabetes mellitus is a metabolic disorder widespread in developing and well-developed countries. It is the sixth leading cause of death in upper middle-income countries in 2015. Many of the synthetic medicine such as sulfonylureas, biguanides, alpha-glucosidase inhibitor and thiazolidinediones are the diabetic agents currently available to control the hyperglycemic condition. Due to the limitations of the drugs, it is required to find a natural alternative for the control of Diabetes Mellitus. Safe and effective antidiabetic agents can be extracted/separated from the traditional medicinal plants. 80% of the population around the Globe depends on traditional medicine. About 350 plants were traditionally used to treat diabetes mellitus. Medicinal plants were assessed for their antidiabetic activity, but these plants were not scientifically evaluated for the presence of an antidiabetic agent. Identification of anti-hyperglycemic agent without any side effects is the need of the hour. WHO has reported tremendous increase of 433million adults with Diabetes Mellitus in 2014, the prevalence of diabetes mellitus in India increased to 7.8% in India. The review focuses on the commonly available and traditionally used medicinal plants of North TamilNadu, India and their role in the control of diabetes mellitus. Introduction Diabetes Mellitus (DM) is a metabolic disorder. It was first described clinically by the Greek physician Aretaeus Cappadocia in 1500 BC " as a condition where Flesh and Bones run together and are siphoned into the urine ". Diabetes mellitus is characterized by hyperglycemic condition along with impaired metabolic functions. Two major types of DM exist; the first type is characterized by insufficient production of insulin and in the other type the target cells do not respond to insulin. The chronic hyperglycemic condition leads to long term damage and failure of target organs. The first WHO global report on diabetes demonstrates that the number of adult living with diabetes has almost quadrupled since 1980 and to 422 million adults in 2014; type 2 diabetes and associated factors such as overweight and obesity were the reason for the dramatic increase. Prevalence of diabetes in India accounts for 7.8% of the adult population [1] Over the past, three decades, the rise in the occurrence of diabetes was dramatic and grows very rapidly in low and middle income countries. The prevalence of diabetes in Tamil Nadu accounts to 13.7% in urban and 7.8% in rural areas in the year 2011 [2]. The synthetic drugs used lead to side effects as well as persistence of hyperglycemic conditions for a longer period of time resulting in serious complications and damages to the heart, blood vessels eyes, kidney and nerves, moreover, increases the risk of heart diseases and stroke [3]. Progressive reduction in β-cell function made it difficult to maintain glycemic control among many diabetic patients [4]. Polytherapy with many hypoglycemic agents to attain better glucose control is a common practice [5]. Many plants have been found to be useful in managing diabetes mellitus. Plants are the major source of drug and are available in the market as extracts directly or indirectly from the plant sources [6]. For the purpose of preventive and curative reasons plants were used as medicine in various parts of the world. Medicinal herbs were used to treat diabetes in large proportion all over the Globe because of the easy availability and affordability [7]. Plant medicines show a potential hypoglycemic activity in diabetes-induced animals. Further, the metabolites from the plant were formulated and were administered to control diabetes mellitus. Novel compounds with antihyperglycemic potential have to be isolated from the plant sources. Studies reveal the role of crude extracts of plants with potential antidiabetic activity in alloxan and streptozotocin-induced diabetic animals. It is required to isolate, purify and characterize the specific compounds with the efficacy to control by reducing blood glucose level and by means of regenerating the damaged β cells of the pancreatic islets for the normal secretion of insulin. The objective of the review is to collect and elucidate the antidiabetic properties of the medicinal plants used by the traditional healers to manage diabetes mellitus.
EVALUATION OF HYPOGLYCEMIC POTENTIAL OF THREE ETHNO-HERBS IN STREPTOZOTOCIN – INDUCED DIABETIC RATS
Catharanthus roseus (L.) G. Don (Apocyanaceae), Momordica charantia L. (Cucurbitaceae) and Syzygium cumini (L) Skeels (Myrtaceae) has been documented as an ethno-hypoglycemic herbs from southeast Rajasthan. Therefore, to evaluate their potential an oral administration of the aqueous extract was administered for 15 days at the concentration of 500 mg /kg body weight in STZ induced hyperglycemic activity. GBC was used in another group to support the results at the concentration of 0.25 mg /kg body weight orally once a day for 15 days. Hypoglycemic activity was observed in all the treated groups revealing significant reduction in blood glucose levels. The results were also found akin to urino-analysis and the body weight tendencies. The performance of Catharanthus roseus was found better as compared to other two and is quite comparable with GBC. Results indicate presence of insulin mimicking natural products in all 3 plants which may contribute for the exploration of new safer drugs.
In-vitro anti-diabetic activity of root and aerial parts of Barleria noctiflora L.f. (Acanthaceae)
The Pharma Innovation Journal, 2018
Barleria noctiflora (Acanthaceae) is a folk medicine used to treat Diabetes. The present investigation deals with morphological and in-vitro anti diabetic study of ethanolic extracts of root and aerial parts of selected plants. The plant material was extracted using soxlet apparatus and ethanol as a solvent. In-vitro anti-diabetic activity was determined by inhibition of α-glucosidase and inhibition of α-amylase studies. The extract showed a significant level of anti-diabetic activity when compared with standards. The results of ethanolic extracts of Barleria noctiflora are in support of traditional uses of the species to reduce blood glucose levels. It is highly likely that long term treatment may achieve the desired results with diabetes mellitus patients.