Thrombocyte Estimation Based on Blood Smear and Its Comparison Using Automated Haematology Analysers (original) (raw)
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Platelet Count Estimation using Unstained and Stained Peripheral Blood Smears: A Comparative Study
JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH, 2018
Introduction: The manual count of platelets using stained smears is the most commonly employed method, although this method has its own limitations. Hence, we planned to use unstained peripheral blood smear for platelet count estimation which is comparatively less time consuming to assess whether it can be used to detect the platelet count in emergency situations like dengue period when workload is more. Aim: To compare the platelet count on unstained and stained peripheral blood smear and to compare the advantages and disadvantages of these two methods.
Importance of Platelet Count in Diagnosis of Dengue Infection
Bioinfo publication, 2018
- Introduction: Dengue is an acute, potentially fatal viral infection that can culminate into dengue hemorrhagic fever and dengue shock syndrome. It has emerged as a notable public health problem in recent decades in terms of the mortality and morbidity associated with it. Specific antibody Ig M and Ig G detection along with the newer parameter NS1 antigen detection has been the main stay of diagnosis. Apart from these parameters, thrombocytopenia is a constant finding and therefore platelet count can be used as an accessory test for diagnosis of dengue infection in the laboratories where resourc es are limited. Materials and Methods: A cross-sectional study was conducted using secondary data of clinically suspected dengue cases reported to the microbiology laboratory during June-August months of 2017 at CIMS teaching hospital, Chamarajanagar. Samples were tested either for NS1or IgM by ELISA depending on duration of fever and platelet counts were obtained. Statistical analysis was done using WHO Epi info software version 3.5.4. Results: Out of 1653 clinically suspected cases, 265(16.03%) were found to be positive for dengue infection either by NS1 or IgM ELISA. Among them 135 (50.95%) were females and majority 211 (79.62) were below the age of 30 years. Proportion of thrombocytopenia was significantly higher in dengue positive cases than in negatives. Conclusion: In resource limited setting thrombocytopenia can be used as predictor of dengue infection for early initiation of therapy to reduce the morbidity and mortality associated with it.
Innovative Publication, 2016
Introduction: Accurate and reproducible platelet counts is essential for patient management. There are many methods for counting platelets. Study was conducted with the objectives of estimation of platelet count indirectly from peripheral blood smear (PBS) on the basis of platelet: red blood cell (RBC) ratio and using automated RBC count; and to verify the reliability of this method in comparison to automated platelet counts. Materials and Methods: A prospective study was conducted in a tertiary care hospital on 200 Ethylene Diamine Tetra Acetic acid (EDTA) anticoagulated blood samples. Samples were evaluated by automated hematology analyser using impedance counting method and by examination of PBS. Number of platelets/1000 RBC in PBS was multiplied by automated RBC count in 10 6 /uL to get an estimate of platelet count in 10 3 /uL. Results: Two sample T test showed no significant difference between the two methods. Pearson correlation of the two methods showed high correlation. Conclusion: Estimation of platelet count on the basis of platelet: RBC ratio is a reliable technique and can be used to validate automated platelet counts.
Evaluation of platelet indices in dengue fever in a tertiary care hospital in Bangladesh
International Journal of Advances in Medicine
Background: Dengue fever is the most rapidly spreading mosquito-borne viral diseases in the world. Some evidence suggests that platelet indices may have diagnostic and prognostic value in Dengue fever. The aim of the study is to evaluate platelet indices and to find their correlation between platelet count in Dengue fever. Methods: This retrospective cross-sectional study was conducted through the use of stored data in Bangabandhu Sheikh Mujib Medical University (BSMMU) dengue cell from July 2019 to September 2019 at department of laboratory medicine and department of internal medicine, BSMMU, Dhaka. Among them, clinical records of 260 cases are preserved in a structured clinical record form (CRF). After ethical clearance from institutional review board (IRB), form clinical records of 260 cases which were preserved in a structured CRF patients who fulfilled the inclusion and exclusion criteria was selected as study population. Results: Platelet indices (MPV and PDW) were statistical...
Platelet Count & Haematocrit as Early Indicators in Acute Dengue Illness
2013
BACKGROUND: The clinical diagnosis of dengue fever (DF), especially in the early phase of illness, is not easy. Laboratory findings such as thrombocytopenia and a rising hematocrit in DF cases are usually observed by day 3 or 4 of the illness. Several studies have revealed a variable prevalence of thrombocytopenia and rising in hematocrit. MATERIALS & METHODS: Blood samples of hospitalised all suspected dengue infected patients in different wards of P.D.U. HOSPITAL, RAJKOT was collected in EDTA anticoagulated bulb were analysed in Central Clinical Laboratory on automated Cell Counter for platelet count and hematocrit (HCT). Simultaneously, Patient’s demographic profile & clinical data & socioeconomic status were recorded. RESULTS: From all of the above data collected & studied, we found that, there is increase in number of cases of DENGUE infection during monsoon season. The disease has higher prevalence in rural area & with low socioeconomic class.The decrease in platelet count & r...
Analysis of Platelet Indices and its correlation with platelet count In Dengue
International Journal of Research in Pharmaceutical Sciences, 2020
Dengue is a global arboviral disease of growing public health concern. Several parameters have been used to detect the infection and severity of dengue. Recent evidence suggests that platelet indices can be used to assess the severity and prognosis of dengue infection. This retrospective study aims at assessing the role of the platelet indices – Mean Platelet Volume (MPV), Platelet Distribution Width, (PDW), P-LCR (Platelet Large Cell Ratio) and Plateletcrit (PCT) and its correlation with the platelet count in 50 Dengue NS1 antigen positive cases and to find the sex ratio and the age-wise distribution of the dengue cases. The indices were obtained using an Automated Hematology analyzer and were compared with the platelet count, considering p-value <0.05 as statistically significant. Thrombocytopenia was found in a majority of the cases and PDW and PLCR were found to be inversely proportional to the platelet count. MPV was found to have no correlation with the platelet count. Henc...
Diagnostic Significance of Platelet Indices in Dengue Fever in Endemic Area
https://www.ijrrjournal.com/IJRR\_Vol.7\_Issue.2\_Feb2020/Abstract\_IJRR0050.html, 2020
Aim and Objective: Dengue fever is one of the most common arboviral mediated outbreaks reported with increased prevalence year after year with considerable morbidity and mortality. The aim to study role of various platelet indices (MPV, PDW, PCT) in early diagnosis of Dengue fever in patient belong from endemic area, patients admitted in MBS Hospital KOTA, a tertiary care hospital in Rajasthan with a diagnosis of Dengue fever according to WHO protocol from December 2018 to January 2020 Methods: Prospective observational study was undertaken among adult patients in a MBS hospital Kota. 132 patients were studied and analysed. These 132 patients were grouped into three according to the platelet count (<20000,20000- 100000, >100000). The patients admitted in our hospital with features suggestive of Dengue fever were taken for the study and diagnosis was confirmed with Dengue IgM ELISA test. CBC were determined using automated counter, five-part haematology analyser in central laboratory of MBS hospital Kota. Result; among 132 patients 22Patients had platelet counts below 20,000 the mean PDW was 17.29fl, 80 cases with platelet counts between 20,000 to 100000, the mean PDW was 16.5fl and 30 cases with platelet counts above 100000 the mean PDW was 13.8fl which showed statistically significant difference between the groups (p value 0.001). The mean MPV values were 9.2fl in 22 patients with platelet counts below 20,000 and 12fl in 80 cases with platelet counts 20,000 to 100000 and 13.7fL in 30 patients with platelet count above 100000. There was statistically significant difference between the groups (p value 0.001). The mean PCT value were 0.03% in 22 patients with platelet counts below 20,000 and 0.1% in 80 cases with platelet counts 20,000 to 100000 and 0.1% in 30 patients with platelet count above 100000. There was statistically significant difference between the groups (p value 0.001). Conclusions: Platelet indices are useful parameters in dengue infection. Other than platelet count, PDW, MPV, plateletcrit are useful to monitor dengue fever. Decrease MPV,PCT and increase PDW is significant in platelet count <100000/mm3 and Platelet indices plays significant role in early predictive diagnosis and severity of dengue in endemic area.
Saudi Journal of Medicine
Dengue fever is public health concerns in Bangladesh, where it occurs in epidemics and has a high mortality in the advanced stages. Clinical features are nonspecific and diagnosis is supported by lab features. Common lab tests include Platelet count, leucocyte count and platelet indices are useful in small rural set ups for early diagnosis and prognosis of dengue. The aim of this study is to evaluate relationship between platelet count, leukocytes and platelet indices. This retrospective cross-sectional study was conducted at Department of Laboratory Medicine in collaboration with Department of Internal Medicine, Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka, Bangladesh from July 2019 to September 2019. Clinical records of 260 cases were preserved in a structured Clinical Record Form (CRF). Only those, with antigen/antibody supported cases was enrolled in the study. Mean neutrophil (%), mean lymphocyte (%) and mean eosinophil (%) count were statistically significant dif...
Evaluation of Platelet Indices in Patients With Dengue Infections
International journal of scientific research, 2016
Recently, novel platelet indices such as MPV, PDW, and P-LCR have been investigated as prospective platelet activation markers. The aim of our study was to investigate the platelet indices in dengue patients and to assess the role of it in the severity dengue infection. The platelet parameters of 200 patients were compared with 200 apparent healthy normal individuals. In our study, there was a significantly lower Platelet count and PCT in DHF group as compared to DF group (P<0.0016 and <0.0166 respectively) and significantly higher PDW in DHF group as compared DF group (P<0.0008). High P-LCR, High PDW and low PCT were more sensitive for DHF. In conclusion, low platelet count, low PCT and high PDW may be used as predictor of severity of Dengue infection. Low MPV, low PCT, high P-LCR and high PDW showing considerable sensitivity and specificity for dengue fever.
innovative publication, 2017
Background: Dengue hemorrhagic fever (DHF) and Dengue Shock Syndrome (DSS) are the two most severe complications of DF and are associated with severe thrombocytopenia and increased vascular permeability. Objective: To determine the relationship between the platelet count and the severity of the dengue fever among paediatric age group. Materials and Methods: This prospective study was conducted for 1year among all Serological confirmed dengue patients admitted at a tertiary care center in Davangere. Platelet count was repeated regularly during the hospital stay and also at the time of discharge. Data was entered in excel sheet and results were analysed using epi-data software. Results: Total 336 were confirmed cases of Dengue. Of these seropositive cases 78.6% had thrombocytopenia (<1L), 32% of the cases were noted in the age group of 6-10years, 50% had platelet counts between 51,000 and 1 lakh. Majority (55.1%) of the cases with severe thrombocytopenia presented with DHF/DSS, the difference of which was found to be statistically significant. Conclusion: Majority of patients with thrombocytopenia were 6-10 years of age. The occurrence of DHF/DSS increased with the severity of thrombocytopenia.