Oxidative stress biomarkers in pulmonary tuberculosis patients in Gombe, North-eastern Nigeria (original) (raw)
Related papers
Assessment of oxidative stress in serum of pulmonary tuberculosis patients
International Journal of Research in Medical Sciences, 2016
Background: Tuberculosis (TB) remains a human health issue and often deadly infectious disease in low-middle income nations. In TB, oxidative stress is a result of tissue inflammation, poor dietary intake of micronutrients due to illness, free radical burst from activated macrophages. This study was conducted prospectively to evaluate the oxidative stress in TB. Methods: The study included 30 newly diagnosed TB positive patients and 30 healthy individuals. Pro-oxidant markers like the thiobarbituric acid reactive species (TBARS) and nitric oxide were studied from serum. Antioxidant parameter like serum total-SH was also assessed. Results: Levels of pro-oxidants were significantly increased whereas antioxidant defense markers were significantly impaired in the TB group. Nitric oxide and TBARS were increased (p<0.0001) where glutathione was decreased (p<0.0001) in TB population compared to healthy controls. Conclusions: Marked oxidative stress were seen in the TB population as compared to the healthy cohort. The role of antioxidant therapy may therefore be evaluated in the management of TB.
Journal of Tuberculosis Research, 2015
Introduction: Non-enzymatic antioxidants are good scavengers of free radicals preventing their overproduction there by reducing the level of oxidative stress. This work was undertaken at Saint Peter TB specialized hospital and Tekle Haimanot health center from March 2012 to May 2013. Aim: To determine changes in Non-Enzymatic Antioxidants and level of oxidative stress of tuberculosis Patients before and after taking anti tuberculosis treatment. Materials and Methods: In this comparative cross sectional study, a total of 210 individuals including: newly diagnosed TB patients as group-I (n = 70), TB patients who completed treatment as group-II (n = 70), and healthy volunteers as group-III (n = 70) were enrolled. Different methods were used to determine the parameters; vit-C (HPLC method), lipid peroxidation (thiobarbuituric acid method), and bilirubin (Colorimetric assay). Results: Vitamin-C (Vit-C) and of group-I showed a significant reduction (p < 0.001) as compared with both group-II and group-III whereas Malondialdehyde (MDA) level was increased. However, the total and direct bilirubin was not different among the groups. In group-III, there was a positive correlation between BMI and serum Vit-C (r = −0.305, p = 0.010). Vit-C showed a negative correlation with serum MDA in all the groups with values (r = −0.265, p = 0.027), (r = −0.389, p = 0.001) and (r = −0.375, p = 0.001) for group-I, group-II and group-III respectively. In addition to this Vit-C was negatively correlated with serum UA (r = −0.285, p = 0.017) in group-I. Conclusion: The findings of the current study suggest that the amount of Vit-C in the newly diagnosed TB patients and those who finished their treatment is much lower than the healthy * Corresponding author. G. Gebretsadik et al. 64 volunteers. In contrast to this, the MDA value was significantly higher both in the newly diagnosed TB patients and TB patients who completed treatment than in healthy volunteers suggesting higher degree of oxidative stress.
Journal of Advances in Medicine and Medical Research
Severe oxidative stress has been reported in Tuberculosis (TB) patients, and this is associated with increased production of free radicals due to phagocyte respiratory burst, malnutrition, and poor immunity. This study investigated the levels of Malondialdehyde (MDA), Superoxide dismutase (SOD) and Selenium (SEL) levels in naïve Tuberculosis (TB) patients and TB patients on treatment. The study comprised of a total of 100 TB patients made up of 57 TB patients on treatment with antituberculosis therapy and 43 naïve TB patients not on treatment. MDA and SOD were analyzed using Enzyme linked Immunosorbent assay technique (ELISA). While selenium and tuberculosis were detected by atomic absorption spectrophotometer and Gene Xpert respectively. The MDA level in TB patients on treatment was significantly higher (p<0.0011) than the MDA level of naïve TB patients. Also, the SOD level of TB patients on treatment was significantly lower (p<0.0426) than the SOD level of naïve TB patients. While the levels of Selenium in TB patients on treatment was significantly higher (p< 0.0002) than the selenium level of naïve TB patients. The study showed an Original Research Article
European Journal of General Medicine
Aim: Several products of cellular (macrophage) activation (i.e. free radicals and cytokines) are potential causes of metabolic disorders in pulmonary tuberculosis (PTB) patients. There is a dearth of information on the levels of neopterin and oxidative metabolites in Nigerian PTB patients. This study therefore assessed the levels of markers of oxidative stress and neopterin in Nigerian PTB patients. Method: Thirty-eight (17 males and 21 females) newly diagnosed PTB-patients and 40 (22 males and 18 females) apparently healthy non-PTB controls volunteered to participate in this study. Diagnostic criteria included sputum acid fast bacilli, chest X-ray and Mantoux test. The levels of superoxide dismutase, plasma albumin, total antioxidant potential, nitric oxide, reduced glutathione, catalase, total plasma peroxide, oxidative stress index, malondialdehyde and neopterin (marker of cellular activation) were determined in them using spectrophotometric methods and enzyme linked immunosorbent assay (ELISA) technique respectively. Result: In PTB-patients, significantly (p<0.05) lower levels of albumin, superoxide dismutase, reduced glutathione, catalase, nitric oxide and total antioxidant potential with significantly (p<0.05) higher levels of total plasma peroxide, malondialdehyde, oxidative stress index and neopterin were observed when compared with the non-PTB controls. The result indicates significantly higher levels of cellular activation, free radical load and oxidative stress with associated antioxidants depletion in PTB patients. Conclusion: Oxidative stress and increased level of neopterin are features of pulmonary tuberculosis. Since most of the antioxidants regulating the free radical load and oxidative stress are micronutrient dependent, micronutrient supplementation may be required as adjuvant therapy in the management of PTB patients.
Oxidative Stress Markers in Tuberculosis and HIV/TB Co-Infection
Journal of Clinical and Diagnostic Research, 2017
In this prospective study all experiments were conducted on Human volunteers were approved by the Institutional Ethical Committee (IEC) of Seth GSM College and KEM Hospital vide letter no. EC/67/2011 dated 27 th June 2011. The samples were collected
THE ROLE OF OXIDATIVE STRESS IN PATIENTS WITH MULTIDRUG-RESISTANT TUBERCULOSIS
drug-resistant tuberculosis is a global challenge of our time. According to WHO, each year half a million new cases of MDR-TB. Tuberculosis drug resistance is characterized by high mortality rates, while the complexity and high cost of its treatment. The most predisposed to the stress are the respiratory system, the brain, the eye, the circulatory system and the reproductive system. Since any body systems are subject to oxidative stress, it has become interesting for us to study these changes in tuberculosis with drug resistance. This review considers the main problem of MDR-TB, тhe role of oxidative stress in patients with multidrug-resistant tuberculosis.
Status of Oxidants and Antioxidants in Pulmonary Tuberculosis with Varying Bacillary Load
Journal of Experimental Sciences, 2011
When ROS production exceeds the detoxification capacity of systemic endogenic antioxidant defense, oxidative stress occurs. Severe oxidative stress has been reported in tuberculosis patients because of malnutrition and poor immunity. However, our knowledge of the antioxidant profile and its relation to lipid peroxidation in tuberculosis is very limited. We analyzed total hundred fresh untreated pulmonary tuberculosis samples with varying bacillary load and controls for oxidative stress markers viz; Malondialdehyde (MDA), Nitric oxide (NO) and Antioxidants viz; Superoxide Dismutase (SOD), Reduced Glutathione (GSH) and Vitamin C by calorimetric methods. The MDA &NO levels were high in AFB+,higher in AFB ++ and with AFB+++ had the highest levels while SOD,GSH &VITC levels were low in AFB+,lower in AFB++ and AFB+++ had the lowest levels. Our findings provide the evidence of enhanced free radical mediated process corresponded with more advanced disease. It might play a role in the pathol...
Objectives: The aim of this study was to assess the total peroxides level and paraoxonase (PON1) activities in patients with Pulmonary tuberculosis (PTB) in Nepalese population. Material and methods: A total of 70 (40 newly diagnosed active PTB and 30 healthy control) subjects were recruited for this study. Anthropometric variables, total peroxide and PON1 activities were determined in control and PTB subjects. Serum glucose, urea, total protein, albumin, globulin and uric acid levels were also determined in the participants. Results: Significant difference in BMI, SBP and DBP was observed between PTB and control subjects (p<0.001, p<0.01 and p<0.01 respectively). Total protein, albumin, TC and HDL were significantly lower in PTB subjects (p<0.05, p <0.001, p<0.01 and p<0.01 respectively). TG and LDL levels were also decreased though not statistically significant. However, the level of uric acid and globulin were significantly increased (p<0.01, p<0.001 respectively).The levels of Total peroxides (µmol H2O2/litre) activity in patients and control were 16.17±0.29 and 12.69±0.32, respectively (p=<0.001). Similarly, the levels of PON1 activity (µmol/min/ml) in patients and control were 109.72±6.43 and 161.55±15.49, respectively (p=0.004). Conclusions: According to this study, we conclude that patients with active PTB are exposed to potent oxidative stress and they have decreased PON1 activity. These predisposal factors play a role in the pathogenesis of pulmonary tuberculosis in a group of Nepalese population which needs more clarification. Keywords: Active pulmonary tuberculosis; PON1 activity; Total oxidant status