Chemical composition and biological activities of a new essential oil chemotype of Tunisian Artemisia herba alba Asso (original) (raw)
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International Journal of Biological Chemistry, 2015
Extraction of essential oils from locally available plant Artemisia Herba-alba was carried out using steam-distillation method. Extracted oils were screened for their chemical composition, antibacterial and antioxidant activities in order to find new metabolite products, which are characterized by a biological activity. Thirty-three constituents, representing 97.54% of the essential oil of Artemisia herba-alba was determined by GC-MS analysis. The main compounds identified are: davanone (42.8%), camphor (15.96%) and thujone (9.63%). The antimicrobial activity of the oil was tested using the agar disc diffusion method, by determining the inhibition zone and the minimum inhibitory concentration. The results have shown a great potential of the antimicrobial activity against the tested strains with an enhanced sensitivity towards the gram-negative strains of Salmonella enteric, Klebsiella and the gram-positive strains of Listeria monocytogenes and Staphylococcus sp. in comparison with the other tested bacteria. The test's results of the essential oil's antioxidant activity, obtained with the anti-radical method 2,2-diphenyl-1-1-picrylhydrazil (DPPH) and the iron-reducing power (FRAP), were compared with those of the ascorbic acid; the usual synthetic antioxidant. The comparison have demonstrated, firstly with the DPPH method a considerable level of antioxidant activity of the essential oil (IC 50 = 17.73±0.29 µg mLG 1) but still lower than that found for the synthetic antioxidant that is the ascorbic acid while the opposite occurred with the iron reduction method, with an higher obtained value of EC 50 =12.53±0.25 µg mLG 1 , for the essential oil's antioxidant activity.
Journal of Agricultural and Food Chemistry, 2005
The compositions of essential oils isolated from the aerial parts of Artemisia absinthium, Artemisia santonicum, and Artemisia spicigera by hydrodistillation were analyzed by GC-MS, and a total of 204 components were identified. The major components of these essential oils were camphor (34.9-1.terpinen-4-ol (3.5-1.2%). The antifungal activities of these essential oils were tested against 11 plant fungi and were compared with that of a commercial antifungal reagent, benomyl. The results showed that all of the oils have potent inhibitory effects at very broad spectrum against all of the tested fungi. Pure camphor and 1,8-cineole, which are the major components of the oils, were also tested for antifungal activity against the same fungal species. Unlike essential oils, these pure compounds were able to show antifungal activity against only some of the fungal species. In addition, the antioxidant and DPPH radical scavenging activities of the essential oils, camphor, and 1,8-cineole were determined in vitro. All of the studied essential oils showed antioxidant activity, but camphor and 1,8-cineole did not.
International Journal of Biosciences | IJB |, 2022
The main objective of this study is to investigate the chemical composition, antioxidant and antimicrobial activities of the essential oil of Artemisia herba-alba Asso. of southern Algeria. Essential oil of Artemisia herbaalba Asso was extracted by hydrodistillation, and their chemical composition was identified by GC/MS Antioxidant activity of essential oil, has been done by using DPPH assay. The antimicrobial activity of essential oil was realised by the agar disc diffusion method. The essential oil extracted from the aerial parts by hydrodistillation was analysed by GC/MS. 39 constituents, representing 99.3% of the oil, were identified, of which the major ones, Thujone (12,759 %), Camphor (7,751%), Eacalyptol (4,525%), Isoborneol (1,119%). IC50 values observed for DPPH essay were 20,27 ±0,767 mg/ml. On the other hand, this oil was found effective against all tested strains; this activity ranged from 15.67±1.53 mm with Listeria innocua CIP 74915. These results provided evidence that the studied plant might indeed be a potential source of natural antioxidant and antimicrobial agents.
Comptes Rendus Chimie, 2010
Eight annual species growing wild in the southern of Tunisia (Diplotaxis simplex, Chrysanthemum coronarium, Matthiola longipetela, Erodium glaucophyllum, Reseda alba, Diplotaxis harra, Senecio gallicus and Papaver rhoeas) were evaluated for their mineral contents and phytochemical screening. The mineral analysis showed that calcium and potassium were the most concentrated minerals (1.21-3.60% and 0.36-3.20% respectively) followed by sodium (0.12-1.38%), magnesium (0.16-0.41%) and phosphorus (0.05-28%). The preliminary phytochemical screening revealed the absence of anthraquinones in all studied plants whereas alkaloids were only present in Papaver rhoeas. Senecio gallicus and Chrysanthemum coronarium were the only species that contained essential oils. All species were found to contain saponins, flavonoids and tannins with the exception of Erodium glaucophyllum and Papaver rhoeas. These results indicate that some of these species may be used as fodder plants for livestock due to their high contents of minerals but the presence of some secondary metabolites may reduce their palatability. In the other hand, these secondary metabolites could be the origin of the medicinal properties of these species.
2017
The essential oils obtained by hydrodistillation from the aerial parts of two endemic species of Artemisia (Asteraceae) collected from the Center of Tunisia (Maknessi), was analysed by GC-MS. Twelve to fourteen components were identified representing more than 80% of the total oil. The essential oil composition and yield showed a variation related to the harvest period. The yield of the essential oils of A. campestris harvested on December was 0.44 % and the one of May was 0.30 %. For A. herba-alba it was 0.5 % for air dried leaves harvested on December and 0.8 % for the one of May. It is observed that the major component in the essential oils for both species is α-thujone followed by cis-sabinol and β-thujone for the extract of December. However, the major compound is β-pinene followed by p-cymen for Artemisia campestris extract of May. The major component of A. campestris α-thujone was absent in the essential oil of the seed stage. This variability could be explained by the phenological stage of the plant. The essential oils of two Artemisia species showed significant antifungal activity against a weak antimicrobial activity.
As part of the valorization of medicinal and aromatic plants in Morocco, we studied the effect of the provenance on the yield, chemical composition and antimicrobial activity of the essential oils of Artemisia herba alba. The essential oils obtained by hydrodistillation from branches were analyzed by GC and GC/MS. The most abundant compounds identified vary according to their origins: á-thujone (17,52 to 48,91%), chrysanthenone (26,64 to 48,11%), 1-4 cineole (0,32 to 9,7%), artemisia alcohol (1,83 to 8,65%), camphor (0,04 to 6,68%) and á-pinene (2,21 to 6,42%).. The influence of the provenance on the antimicrobial activity of these essential oils was also highlighted.
Separations
In Morocco, the endemic Artemisia herba-alba is well known by its traditional uses and health benefits. The search for natural, safe, and effective antibacterial and antifungal agents from plants is in high demand due to microbial and fungal resistance to conventional synthetic antibiotics and antifungal drugs. In this study, the A. herba-alba was collected from the region of Fez-Boulemane during the periods of March, June, and September. Essential oils (EOs) were extracted from the aerial part of the plant by the hydrodistillation method. The chemical constituents were determined using GC-MS as analytical tools. The antimicrobial activities of different oils were tested using the macrodilution method. The results showed the difference in the yields between the three EOs (0.49, 1.74, 1.30% (mL/100 g)), respectively, as well as in their corresponding chemical compositions. The main constituents revealed by GC-MS are higher contents of oxygenated monoterpenes (84.7, 84.4, 81%), such a...
Biodiversitas, 2022
Artemisia herba-alba Asso (Asteraceae family) is widespread in the semi-arid and arid steppes of North Africa. This plant is used for traditional treatment. The present study aims to investigate the chemical composition, antioxidant and antimicrobial activities of A. herba-alba essential oil found in southwest Algeria (Boussaâda region). Artemisia herba-alba essential oil was obtained by hydrodistillation method, and its chemical composition was identified by using GC/MS analysis. In addition, the antioxidant activity of the extracted essential oil was determined using the DPPH assay. The antimicrobial activity of the essential oil was determined by the agar disc diffusion method. The essential oil extracted from the aboveground portions by hydrodistillation was analyzed by GC/MS. 38 components were identified, making up 69.37% of the oil, the most important of which is thujone (9.875%), camphor (3.762%), cis-pmenthadien-1-of (3.572%), and isoborneol (2.334%). The observed IC50 values of the DPPH assay were 7.31 ± 0.088 mg/mL. On the other hand, this oil was active against all strains tested, this activity varied from 12.77 ± 0.510 mm on Listeria innocua CIP 74915. These results demonstrate that the plant tested could be a potential source of natural antioxidants and antimicrobial agents.
International Journal of Food Properties, 2015
Total phenols, flavonoids, flavonols, and flavanols of the methanolic extract of the aerial part of Artemisia herba-alba were determined. The extract was analyzed by liquid chromatography with photodiode array coupled with electrospray ionisation mass spectrometry and allowed to identify of 10 phenolic compounds. Apigenin-6-C-glycosyl flavonoids and caffeoylquinic acids were identified. Chlorogenic acid and 1,4 dicaffeoylquinic acid being the major constituents. The essential oil obtained by hydrodistillation was analyzed by gas chromatography-mass spectrometry. Twenty-three compounds, representing 97.8% of the total oil, were identified. The most abundant components were βthujone (41.9%), α-thujone (18.4%), and camphor (13.2%). Methanolic extract and essential oil exhibited a considerable antioxidant activity as evaluated by 2,2-diphenyl-pycrilhydrazil hydrate scavenging activity, reducing power, β-carotene bleaching test, and chelating ability. The methanolic extract was found to be more efficient, while the essential oil exhibited the highest acetylcholinesterase inhibitory activity. Analysis of the antibacterial activity showed that A. herba-alba methanolic extract and essential oil are efficient against gram positive and gram negative bacteria.
Phytochemistry, 2008
The chemical composition of essential oils isolated from aerial parts of seven wild sages from Western Canada -Artemisia absinthium L., Artemisia biennis Willd., Artemisia cana Pursh, Artemisia dracunculus L., Artemisia frigida Willd., Artemisia longifolia Nutt. and Artemisia ludoviciana Nutt., was investigated by GC-MS. A total of 110 components were identified accounting for 71.0-98.8% of the oil composition. High contents of 1,8-cineole (21.5-27.6%) and camphor (15.9-37.3%) were found in Artemisia cana, A. frigida, A. longifolia and A. ludoviciana oils. The oil of A. ludoviciana was also characterized by a high content of oxygenated sesquiterpenes with a 5-ethenyltetrahydro-5-methyl-2-furanyl moiety, of which davanone (11.5%) was the main component identified. A. absinthium oil was characterized by high amounts of myrcene (10.8%), trans-thujone (10.1%) and trans-sabinyl acetate (26.4%). A. biennis yielded an oil rich in (Z)beta-ocimene (34.7%), (E)-beta-farnesene (40.0%) and the acetylenes (11.0%) (Z)-and (E)-en-yn-dicycloethers. A. dracunculus oil contained predominantly phenylpropanoids such as methyl chavicol (16.2%) and methyl eugenol (35.8%). Artemisia oils had inhibitory effects on the growth of bacteria (Escherichia coli, Staphylococcus aureus, and Staphylococcus epidermidis), yeasts (Candida albicans, Cryptococcus neoformans), dermatophytes (Trichophyton rubrum, Microsporum canis, and Microsporum gypseum), Fonsecaea pedrosoi and Aspergillus niger. A. biennis oil was the most active against dermatophytes, Cryptococcus neoformans, Fonsecaea pedrosoi and Aspergillus niger, and A. absinthium oil the most active against Staphylococcus strains. In addition, antioxidant (beta-carotene/linoleate model) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities were determined, and weak activities were found for these oils.